Colocalization of human immunodeficiency virus and hepatitis C virus in peripheral blood cells by fluorescent in situ hybridization

Abstract Chromosomal abnormalities, namely deletion 11q-,13q-, 17p- and trisomy 12, have prognostic significance for patients with chronic lymphocytic leukemia (CLL). Several studies have demonstrated that the interphase fluorescence in situ hybridization technique (I-FISH) in CLL identifies such genomic aberrations in a higher frequency than classical karyotyping, including stimulated cultures using B-cell specific mitogens. However, there appears to be no information in the literature comparing I-FISH on non-cultured and cultured cells in CLL. A total of 56 samples from 49 patients with CLL were studied using commercially available probes for chromosomes 11q22.3(ATM), 13q14(13S272), 17p13(p53) and 12 centromere(D12Z3). We compared the results obtained by I-FISH-PBMC and those by interphase fluorescence in situ hybridization on TPA-stimulated peripheral blood cells (I-FISH-TPA) used for conventional cytogenetics in order to evaluate the usefulness of I-FISH-TPA. The proportion of abnormal nuclei obtained with the I-FISH-TPA was higher than that found with I-FISH-PBMC (P<0.001). Consequently, 15 cases with a negative or borderline result by I-FISH-PBMC became positive by I-FISH-TPA for deletion 11q- (n=2), 13q- (n= 9) and trisomy 12 (n=4). In all but one of these, chromosomal abnormalities were reconfirmed by either metaphase-FISH or conventional G-banding. Disease detection thus increased from 51% with I-FISH-PBMC to 78% with I-FISH-TPA. Interestingly, all 15 cases which reached the diagnostic thresholds for deletion 11q-,13q- and trisomy 12 had a slight lymphocytosis. An absolute lymphocyte count <8.7×109/l was found to be the critical threshold (P=0.037) below which I-FISH-TPA should be performed rather than I-FISH-PBMC. We have shown that I-FISH-TPA can not only detect a higher proportion of abnormal interphase nuclei but can also identify abnormal CLL cases which may be overlooked by I-FISH-PBMC, especially those with low absolute lymphocyte counts. I-FISH-TPA is thus a reliable technique for clinical diagnostics in CLL.

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Human immunodeficiency virus type 1 mRNA expression in peripheral blood cells predicts disease progression independently of the numbers of CD4+ lymphocytes.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.91.3.1104 ◽

1994 ◽

Vol 91 (3) ◽

pp. 1104-1108 ◽

Cited By ~ 68

Author(s):

K. Saksela ◽

C. Stevens ◽

P. Rubinstein ◽

D. Baltimore

Keyword(s):

Human Immunodeficiency Virus ◽

Mrna Expression ◽

Disease Progression ◽

Human Immunodeficiency Virus Type ◽

Peripheral Blood ◽

Blood Cells ◽

Peripheral Blood Cells ◽

Immunodeficiency Virus ◽

Cd4 Lymphocytes

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Fluorescence in situ hybridization analysis of peripheral blood cells in Pearson marrow-pancreas syndrome

The Journal of Pediatrics ◽

10.1067/mpd.2001.116296 ◽

2001 ◽

Vol 139 (3) ◽

pp. 452-455 ◽

Cited By ~ 7

Author(s):

Itaru Yanagihara ◽

Koji Inui ◽

Keiko Yanagihara ◽

Yong-Dong Park ◽

Junko Tanaka ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Peripheral Blood ◽

Blood Cells ◽

Hybridization Analysis ◽

Peripheral Blood Cells

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Impact of Nucleic Acid Testing for Human Immunodeficiency Virus and Hepatitis C Virus on Blood Product Availability, Outdating, and Patient Safety

Archives of Pathology & Laboratory Medicine ◽

10.5858/2002-126-1463-ionatf ◽

2002 ◽

Vol 126 (12) ◽

pp. 1463-1466

Author(s):

Laurence A. Sherman

Keyword(s):

Human Immunodeficiency Virus ◽

Patient Safety ◽

Hepatitis C Virus ◽

Hepatitis C ◽

Nucleic Acid ◽

Red Blood Cells ◽

Blood Cells ◽

Nucleic Acid Testing ◽

Immunodeficiency Virus ◽

The Impact

Abstract Context.—Limited data are available about the impact of nucleic acid testing for human immunodeficiency virus and hepatitis C virus in donated blood as part of a nationwide investigational study that affected greater than 90% of the blood supply. Objective.—To assess the impact of nucleic acid testing on supply, outdating, and patient safety. Design.—Participants in the College of American Pathologists 2001 American Association of Blood Banks/College of American Pathologists Viral Marker C survey were asked questions about supply, outdating, and implementation of a full quarantine of blood pending nucleic acid testing results. The number of respondents for each question ranged from 197 to 219 for blood centers and from 462 to 504 for hospitals. Results.—Shortages were more common for platelets (29% and 23% of blood centers and hospitals, respectively) than for red blood cells (13%, 11%). Similarly, outdating of platelets (13%, 11%) was more common than outdating of red blood cells; outdating of red blood cells was negligible for both blood centers and hospitals. Forty-two percent of blood centers did not meet the mid 2000 target date for quarantining red blood cells, and 18% were not quarantining as of September 2001. The hospital figures were 66% not quarantining in mid 2000 and 39% not quarantining as of September 2001. Higher proportions of centers and hospitals were not quarantining platelets at these 2 dates. Conclusions.—Unfavorable trends in both blood shortages and outdating were attributed to nucleic acid testing. Greater effects may have been masked by delayed implementation of full quarantine nationwide. This delay meant continued patient risk, and lack of full benefit, in a trial that was in effect a national standard. In the future, added systems will be needed for similar new endeavors to ensure uniformity of care and to avoid shortages.

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