Transcriptome Profiling, Biochemical and Physiological Analyses Provide New Insights towards Drought Tolerance in Nicotiana tabacum L.

Drought stress is one of the main factors limiting crop production, which provokes a number of changes in plants at physiological, anatomical, biochemical and molecular level. To unravel the various mechanisms underpinning tobacco (Nicotiana tabacum L.) drought stress tolerance, we conducted a comprehensive physiological, anatomical, biochemical and transcriptome analyses of three tobacco cultivars (i.e., HongHuaDaJinYuan (H), NC55 (N) and Yun Yan-100 (Y)) seedlings that had been exposed to drought stress. As a result, H maintained higher growth in term of less reduction in plant fresh weight, dry weight and chlorophyll content as compared with N and Y. Anatomical studies unveiled that drought stress had little effect on H by maintaining proper leaf anatomy while there were significant changes in the leaf anatomy of N and Y. Similarly, H among the three varieties was the least affected variety under drought stress, with more proline content accumulation and a powerful antioxidant defense system, which mitigates the negative impacts of reactive oxygen species. The transcriptomic analysis showed that the differential genes expression between HongHuaDaJinYuan, NC55 and Yun Yan-100 were enriched in the functions of plant hormone signal transduction, starch and sucrose metabolism, and arginine and proline metabolism. Compared to N and Y, the differentially expressed genes of H displayed enhanced expression in the corresponding pathways under drought stress. Together, our findings offer insights that H was more tolerant than the other two varieties, as evidenced at physiological, biochemical, anatomical and molecular level. These findings can help us to enhance our understanding of the molecular mechanisms through the networks of various metabolic pathways mediating drought stress adaptation in tobacco.

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Evolutionary Origins of Drought Tolerance in Spermatophytes

Frontiers in Plant Science ◽

10.3389/fpls.2021.655924 ◽

2021 ◽

Vol 12 ◽

Author(s):

Alexander M. C. Bowles ◽

Jordi Paps ◽

Ulrike Bechtold

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Crop Production ◽

Molecular Mechanisms ◽

Complex Trait ◽

Binary Trait ◽

Gene Gain ◽

Plant Kingdom ◽

Drought Tolerant ◽

Future Production

It is commonly known that drought stress is a major constraint limiting crop production. Drought stress and associated drought tolerance mechanisms are therefore under intense investigation with the view to future production of drought tolerant crops. With an ever-growing population and variable climate, novel approaches need to be considered to sustainably feed future generations. In this context, definitions of drought tolerance are highly variable, which poses a major challenge for the systematic assessment of this trait across the plant kingdom. Furthermore, drought tolerance is a polygenic trait and understanding the evolution of this complex trait may inform us about patterns of gene gain and loss in relation to diverse drought adaptations. We look at the transition of plants from water to land, and the role of drought tolerance in enabling this transition, before discussing the first drought tolerant plant and common drought responses amongst vascular plants. We reviewed the distribution of a combined “drought tolerance” trait in very broad terms to encompass different experimental systems and definitions used in the current literature and assigned a binary trait “tolerance vs. sensitivity” in 178 extant plant species. By simplifying drought responses of plants into this “binary” trait we were able to explore the evolution of drought tolerance across the wider plant kingdom, compared to previous studies. We show how this binary “drought tolerance/sensitivity” trait has evolved and discuss how incorporating this information into an evolutionary genomics framework could provide insights into the molecular mechanisms underlying extreme drought adaptations.

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Effects of night temperature on the growth of Nicotiana tabacum

Australian Journal of Experimental Agriculture ◽

10.1071/ea9670078 ◽

1967 ◽

Vol 7 (24) ◽

pp. 78 ◽

Cited By ~ 1

Author(s):

JM Hipkinson

Keyword(s):

Nicotiana Tabacum ◽

Leaf Growth ◽

Dry Weight ◽

Physiological Adaptation ◽

Flower Initiation ◽

Night Temperature ◽

Temperature Optimum ◽

Leaf Production ◽

Node Number ◽

Nicotiana Tabacum L

Four varieties of flue-cured tobacco, Nicotiana tabacum L., showed similar responses to night temperatures of 10�, 16�, 22�, and 28�C, with a day temperature of 27�C. Leaf growth rates and leaf production rates were approximately the same at night temperatures of 28� and 22�C, and were successively lower at 16� and 10�C. Dry weight accumulation was greatest at 22�. The node number of flower initiation increased with increasing night temperature over the whole range. There was no evidence of either a shift in the night temperature optimum or a physiological adaptation to low temperature during development.

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Comparative Analysis of Differential drought Stress-induced Suppression of and Recovery in Carbon Dioxide Fixation: Stomatal and Non-stomatal Limitation in Nicotiana tabacum L.

Journal of Plant Physiology ◽

10.1016/s0176-1617(11)80426-8 ◽

1993 ◽

Vol 142 (3) ◽

pp. 296-306 ◽

Cited By ~ 27

Author(s):

Leon van Rensburg ◽

Gert H.J. Krüger

Keyword(s):

Carbon Dioxide ◽

Comparative Analysis ◽

Drought Stress ◽

Nicotiana Tabacum ◽

Carbon Dioxide Fixation ◽

Stomatal Limitation ◽

Nicotiana Tabacum L

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Co-inoculation of rhizobacteria promotes growth, yield, and nutrient contents in soybean and improves soil enzymes and nutrients under drought conditions

Scientific Reports ◽

10.1038/s41598-021-01337-9 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Dilfuza Jabborova ◽

Annapurna Kannepalli ◽

Kakhramon Davranov ◽

Abdujalil Narimanov ◽

Yuriy Enakiev ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Plant Growth ◽

Soil Enzymes ◽

Crop Production ◽

Growth Yield ◽

Dry Weight ◽

Stress Conditions ◽

Abiotic Factor ◽

Drought Conditions

AbstractDrought stress is the major abiotic factor limiting crop production. Co-inoculating crops with nitrogen fixing bacteria and plant growth-promoting rhizobacteria (PGPR) improves plant growth and increases drought tolerance in arid or semiarid areas. Soybean is a major source of high-quality protein and oil for humans. It is susceptible to drought stress conditions. The co-inoculation of drought-stressed soybean with nodulating rhizobia and root-colonizing, PGPR improves the root and the shoot growth, formation of nodules, and nitrogen fixation capacity in soybean. The present study was aimed to observe if the co-inoculation of soybean (Glycine max L. (Merr.) nodulating with Bradyrhizobium japonicum USDA110 and PGPR Pseudomonas putida NUU8 can enhance drought tolerance, nodulation, plant growth, and nutrient uptake under drought conditions. The results of the study showed that co-inoculation with B. japonicum USDA110 and P. putida NUU8 gave more benefits in nodulation and growth of soybean compared to plants inoculated with B. japonicum USDA110 alone and uninoculated control. Under drought conditions, co-inoculation of B. japonicum USDA 110 and P. putida NUU8 significantly enhanced the root length by 56%, shoot length by 33%, root dry weight by 47%, shoot dry weight by 48%, and nodule number 17% compared to the control under drought-stressed. Co-inoculation with B. japonicum, USDA 110 and P. putida NUU8 significantly enhanced plant and soil nutrients and soil enzymes compared to control under normal and drought stress conditions. The synergistic use of B. japonicum USDA110 and P. putida NUU8 improves plant growth and nodulation of soybean under drought stress conditions. The results suggested that these strains could be used to formulate a consortium of biofertilizers for sustainable production of soybean under drought-stressed field conditions.

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Transcriptome profiling reveals the effects of drought tolerance in Giant Juncao

BMC Plant Biology ◽

10.1186/s12870-020-02785-7 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Jing Zhou ◽

Siqi Chen ◽

Wenjiao Shi ◽

Rakefet David-Schwartz ◽

Sutao Li ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Leucine Zipper ◽

Molecular Mechanisms ◽

Normal Growth ◽

Transcriptome Profiling ◽

Fatty Acyl ◽

Transferase Activity ◽

Forage Production ◽

Basic Leucine Zipper

Abstract Background Giant Juncao is often used as feed for livestock because of its huge biomass. However, drought stress reduces forage production by affecting the normal growth and development of plants. Therefore, investigating the molecular mechanisms of drought tolerance will provide important information for the improvement of drought tolerance in this grass. Results A total of 144.96 Gb of clean data was generated and assembled into 144,806 transcripts and 93,907 unigenes. After 7 and 14 days of drought stress, a total of 16,726 and 46,492 differentially expressed genes (DEGs) were observed, respectively. Compared with normal irrigation, 16,247, 23,503, and 11,598 DEGs were observed in 1, 5, and 9 days following rehydration, respectively. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed abiotic stress-responsive genes and pathways related to catalytic activity, methyltransferase activity, transferase activity, and superoxide metabolic process. We also identified transcription factors belonging to several families, including basic helix-loop-helix (bHLH), WRKY, NAM (no apical meristem), ATAF1/2 and CUC2 (cup-shaped cotyledon) (NAC), fatty acyl-CoA reductase (FAR1), B3, myeloblastosis (MYB)-related, and basic leucine zipper (bZIP) families, which are important drought-rehydration-responsive proteins. Weighted gene co-expression network analysis was also used to analyze the RNA-seq data to predict the interrelationship between genes. Twenty modules were obtained, and four of these modules may be involved in photosynthesis and plant hormone signal transduction that respond to drought and rehydration conditions. Conclusions Our research is the first to provide a more comprehensive understanding of DEGs involved in drought stress at the transcriptome level in Giant Juncao with different drought and recovery conditions. These results may reveal insights into the molecular mechanisms of drought tolerance in Giant Juncao and provide diverse genetic resources involved in drought tolerance research.

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Comparison of selenite and selenate in alleviation of drought stress in Nicotiana tabacum L.

Chemosphere ◽

10.1016/j.chemosphere.2021.132136 ◽

2022 ◽

Vol 287 ◽

pp. 132136

Author(s):

Dan Han ◽

Shuxin Tu ◽

Zhihua Dai ◽

Wuxing Huang ◽

Wei Jia ◽

...

Keyword(s):

Drought Stress ◽

Nicotiana Tabacum ◽

Nicotiana Tabacum L

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Drought Stress Study on Nicotiana tabacum L., “Baladi”, an In Vitro Experimental Model

Agriculture ◽

10.3390/agriculture11090845 ◽

2021 ◽

Vol 11 (9) ◽

pp. 845

Author(s):

Maria-Mihaela Antofie ◽

Camelia Sava Sand

Keyword(s):

Drought Stress ◽

Nicotiana Tabacum ◽

Multiplication Rate ◽

Sod Activity ◽

Nicotiana Tabacum L ◽

Stress Study ◽

Food And Feed ◽

Peg Treatment ◽

Set Up

Crops drought tolerance is a trait of outmost importance for agriculture especially today when climate change is affecting more the production for food and feed. The scope of this article is to evaluate in vitro drought stress response of Nicotiana tabacum L., “Baladi”. The experiment was set up for four successive stages starting with in vitro seedling development, hypocotyl cultivation, three generations of micropropagation, pre-acclimatization and acclimatization. The effect of abscisic acid (ABA) and/or polyethylene-glycol 6000 (PEG) on tobacco hypocotyl caulogenesis and micropropagation were investigated. Superoxide-dismutases (SODs) and peroxidases (POXs) are more active and different isoforms patterns have been identified compared to the control for cualogenesis. A decrease of internodes length and a higher shoots multiplication rate were observed. However, under PEG treatment plantlets expressed hyperhydration and ceased rooting. Pre-treatments effects study of ABA and/or PEG were finalized in acclimatization phase for 18 tobacco clones. A summary of our results revealed that ABA and/or PEG induce among others a higher oxidative stress compared to the control in the first stage that is not maintained for all clones until acclimatization. Certain clones expressed a lower SOD activity compared to the control during acclimatization but maintaining higher POX activity.

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Phosphoenolpyruvate carboxylase, NADP-malic enzyme, and pyruvate, phosphate dikinase are involved in the acclimation of Nicotiana tabacum L. to drought stress

Journal of Plant Physiology ◽

10.1016/j.jplph.2013.10.017 ◽

2014 ◽

Vol 171 (5) ◽

pp. 19-25 ◽

Cited By ~ 42

Author(s):

Veronika Doubnerová Hýsková ◽

Lucia Miedzińska ◽

Jana Dobrá ◽

Radomira Vankova ◽

Helena Ryšlavá

Keyword(s):

Drought Stress ◽

Nicotiana Tabacum ◽

Phosphoenolpyruvate Carboxylase ◽

Malic Enzyme ◽

Pyruvate Phosphate Dikinase ◽

Nicotiana Tabacum L

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The CaCIPK3 gene positively regulates drought tolerance in pepper

Horticulture Research ◽

10.1038/s41438-021-00651-7 ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Xiao Ma ◽

Yang Li ◽

Wen-Xian Gai ◽

Chuang Li ◽

Zhen-Hui Gong

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Antioxidant Defense ◽

Molecular Mechanisms ◽

Ectopic Expression ◽

Antioxidant Defense System ◽

Pcr Analysis ◽

Defense System ◽

Interacting Protein ◽

Transient Overexpression

AbstractDrought stress is a major agricultural problem restricting the growth, development, and productivity of plants. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) significantly influence the plant response to different stresses. However, the molecular mechanisms of CBL–CIPK in the drought stress response of pepper are still unknown. Here, the function of CaCIPK3 in the regulation of drought stress in pepper (Capsicum annuum L.) was explored. Transcriptomic data and quantitative real-time PCR (qRT-PCR) analysis revealed that CaCIPK3 participates in the response to multiple stresses. Knockdown of CaCIPK3 in pepper increased the sensitivity to mannitol and methyl jasmonate (MeJA). Transient overexpression of CaCIPK3 improved drought tolerance by enhancing the activities of the antioxidant system and positively regulating jasmonate (JA)-related genes. Ectopic expression of CaCIPK3 in tomato also improved drought and MeJA resistance. As the CaCIPK3-interacting partner, CaCBL2 positively influenced drought resistance. Additionally, CaWRKY1 and CaWRKY41 directly bound the CaCIPK3 promoter to influence its expression. This study shows that CaCIPK3 acts as a positive regulator in drought stress resistance via the CBL–CIPK network to regulate MeJA signaling and the antioxidant defense system.

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