Phosphoenolpyruvate carboxylase, NADP-malic enzyme, and pyruvate, phosphate dikinase are involved in the acclimation of Nicotiana tabacum L. to drought stress

2014 ◽  
Vol 171 (5) ◽  
pp. 19-25 ◽  
Author(s):  
Veronika Doubnerová Hýsková ◽  
Lucia Miedzińska ◽  
Jana Dobrá ◽  
Radomira Vankova ◽  
Helena Ryšlavá
Keyword(s):  
Drought Stress ◽  
Nicotiana Tabacum ◽  
Phosphoenolpyruvate Carboxylase ◽  
Malic Enzyme ◽  
Pyruvate Phosphate Dikinase ◽  
Nicotiana Tabacum L

Possible involvement of phosphoenolpyruvate carboxylase and NAD-malic enzyme in response to drought stress. A case study: a succulent nature of the C4-NAD-ME type desert plant, Salsola lanata (Chenopodiaceae)

2017 ◽  
Vol 44 (12) ◽  
pp. 1219
Author(s):  
Zhibin Wen ◽  
Mingli Zhang
Keyword(s):  
Enzyme Activity ◽  
Drought Stress ◽  
Phosphoenolpyruvate Carboxylase ◽  
Malic Enzyme ◽  
C4 Plants ◽  
Leaf Water Content ◽  
Transcriptional Level ◽  
Desert Plant ◽  
Severe Stress ◽  
Real Time Quantitative Pcr

The co-ordination between the primary carboxylating enzyme phosphoenolpyruvate carboxylase (PEPC) and the further decarboxylating enzymes is crucial to the efficiency of the CO2-concentrating mechanism in C4 plants, and investigations on more types of C4 plants are needed to fully understand their adaptation mechanisms. In this study we investigated the effect of drought on carboxylating enzyme PEPC, and the further decarboxylating NAD-malic enzyme (NAD-ME) of Salsola lanata Pall. (Chenopodiaceae) – an annual succulent C4-NAD-ME subtype desert plant. We investigated enzyme activity at the transcriptional level with real-time quantitative PCR and at the translational level by immunochemical methods, and compared S. lanata with other forms of studied C4 plants under drought stress. Results showed that only severe stress limited PEPC enzyme activity (at pH 8.0) of S. lanata significantly. Considering that PEPC enzyme activity (at pH 8.0) was not significantly affected by phosphorylation, the decrease of PEPC enzyme activity (at pH 8.0) of S. lanata under severe stress may be related with decreased PEPC mRNA. The suggestion of increased phosphorylation of the PEPC enzyme in plants under moderate stress was supported by the ratio of PEPC enzyme activity at pH 7.3/8.0, as PEPC enzyme is inhibited by L-malate and the evidence of the 50% inhibiting concentration of L-malate. NAD-ME activity decreased significantly under moderate and severe stress, and coincided with a change of leaf water content rather than the amount of α-NAD-ME mRNA and protein. Leaf dehydration may cause the decrease of NAD-ME activity under water stress. Compared with other C4 plants, the activities of PEPC and NAD-ME of S. lanata under drought stress showed distinct features.

scholarly journals Transcriptome Profiling, Biochemical and Physiological Analyses Provide New Insights towards Drought Tolerance in Nicotiana tabacum L.

Genes ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1041 ◽  
Author(s):  
Rayyan Khan ◽  
Peilu Zhou ◽  
Xinghua Ma ◽  
Lei Zhou ◽  
Yuanhua Wu ◽  
...  
Keyword(s):  
Drought Stress ◽  
Nicotiana Tabacum ◽  
Leaf Anatomy ◽  
Crop Production ◽  
Molecular Mechanisms ◽  
Molecular Level ◽  
Transcriptome Profiling ◽  
Dry Weight ◽  
Antioxidant Defense System ◽  
Nicotiana Tabacum L

Drought stress is one of the main factors limiting crop production, which provokes a number of changes in plants at physiological, anatomical, biochemical and molecular level. To unravel the various mechanisms underpinning tobacco (Nicotiana tabacum L.) drought stress tolerance, we conducted a comprehensive physiological, anatomical, biochemical and transcriptome analyses of three tobacco cultivars (i.e., HongHuaDaJinYuan (H), NC55 (N) and Yun Yan-100 (Y)) seedlings that had been exposed to drought stress. As a result, H maintained higher growth in term of less reduction in plant fresh weight, dry weight and chlorophyll content as compared with N and Y. Anatomical studies unveiled that drought stress had little effect on H by maintaining proper leaf anatomy while there were significant changes in the leaf anatomy of N and Y. Similarly, H among the three varieties was the least affected variety under drought stress, with more proline content accumulation and a powerful antioxidant defense system, which mitigates the negative impacts of reactive oxygen species. The transcriptomic analysis showed that the differential genes expression between HongHuaDaJinYuan, NC55 and Yun Yan-100 were enriched in the functions of plant hormone signal transduction, starch and sucrose metabolism, and arginine and proline metabolism. Compared to N and Y, the differentially expressed genes of H displayed enhanced expression in the corresponding pathways under drought stress. Together, our findings offer insights that H was more tolerant than the other two varieties, as evidenced at physiological, biochemical, anatomical and molecular level. These findings can help us to enhance our understanding of the molecular mechanisms through the networks of various metabolic pathways mediating drought stress adaptation in tobacco.

scholarly journals Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity

2009 ◽  
Vol 10 (8) ◽  
pp. 3583-3598 ◽  
Author(s):  
Veronika Doubnerová ◽  
Karel Müller ◽  
Noemi Čeřovská ◽  
Helena Synková ◽  
Petra Spoustová ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Potato Virus ◽  
Malic Enzyme ◽  
Potato Virus Y ◽  
Nicotiana Tabacum L

Comparison of selenite and selenate in alleviation of drought stress in Nicotiana tabacum L.

Chemosphere ◽  
2022 ◽  
Vol 287 ◽  
pp. 132136
Author(s):  
Dan Han ◽  
Shuxin Tu ◽  
Zhihua Dai ◽  
Wuxing Huang ◽  
Wei Jia ◽  
...  
Keyword(s):  
Drought Stress ◽  
Nicotiana Tabacum ◽  
Nicotiana Tabacum L

scholarly journals Drought Stress Study on Nicotiana tabacum L., “Baladi”, an In Vitro Experimental Model

Agriculture ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 845
Author(s):  
Maria-Mihaela Antofie ◽  
Camelia Sava Sand
Keyword(s):  
Drought Stress ◽  
Nicotiana Tabacum ◽  
Multiplication Rate ◽  
Sod Activity ◽  
Nicotiana Tabacum L ◽  
Stress Study ◽  
Food And Feed ◽  
Peg Treatment ◽  
Set Up

Crops drought tolerance is a trait of outmost importance for agriculture especially today when climate change is affecting more the production for food and feed. The scope of this article is to evaluate in vitro drought stress response of Nicotiana tabacum L., “Baladi”. The experiment was set up for four successive stages starting with in vitro seedling development, hypocotyl cultivation, three generations of micropropagation, pre-acclimatization and acclimatization. The effect of abscisic acid (ABA) and/or polyethylene-glycol 6000 (PEG) on tobacco hypocotyl caulogenesis and micropropagation were investigated. Superoxide-dismutases (SODs) and peroxidases (POXs) are more active and different isoforms patterns have been identified compared to the control for cualogenesis. A decrease of internodes length and a higher shoots multiplication rate were observed. However, under PEG treatment plantlets expressed hyperhydration and ceased rooting. Pre-treatments effects study of ABA and/or PEG were finalized in acclimatization phase for 18 tobacco clones. A summary of our results revealed that ABA and/or PEG induce among others a higher oxidative stress compared to the control in the first stage that is not maintained for all clones until acclimatization. Certain clones expressed a lower SOD activity compared to the control during acclimatization but maintaining higher POX activity.

Assessing the drought-resistance adaptive advantage of some anatomical and physiological features in Nicotiana tabacum

1994 ◽  
Vol 72 (10) ◽  
pp. 1445-1454 ◽  
Author(s):  
L. van Rensburg ◽  
G. H. J. Kruger ◽  
H. Krüger
Keyword(s):  
Drought Stress ◽  
Nicotiana Tabacum ◽  
Energy Dissipation ◽  
Drought Tolerance ◽  
Rate Constant ◽  
Drought Tolerant ◽  
Nicotiana Tabacum L ◽  
Carotenoid Composition ◽  
Leaf Movements ◽  
Nonradiative Energy Dissipation

As part of an extensive research programme that attempts to explain and quantify the known difference in drought tolerance of four Nicotiana tabacum L. cultivars, some aspects that may contribute towards the avoidance and tolerance of drought stress were investigated to evaluate overall resistance. The results presented emphasize the adaptive significance of effective leaf movements in determining drought tolerance, by reducing or preventing damage to the photosynthetic system caused by photoinhibition and direct heat damage. Leaf movement seemed to be achieved with greater efficiency by the drought-tolerant cultivars GS46 and Elsoma. Drought stress specifically influenced the carotenoid composition. A strong quantitative correlation existed between the formation of zeaxanthin in the xanthophyll cycle and the type of fluorescence quenching, which is indicative of nonradiative energy dissipation. The latter occurred to a lesser extent in the drought-tolerant cultivars GS46 and Elsoma. This phenomenon also indicated that the rate constant for nonradiative energy dissipation in tobacco remains relatively small in relation to the rate constant for fluorescence during drought stress. Furthermore, irrespective of their drought tolerance, it would seem that tobacco plants have a capability towards starch overproduction, though this was less pronounced in the drought-sensitive cultivars TL33 and CDL28. Owing to anatomical differences, resistance to water flow varied between the respective cultivars, as did the percentage intercellular spaces, both of which correlated positively with the slower decrease in water use efficiency and faster recovery upon rehydration of the drought-tolerant cultivars. Key words: carotenoid composition, chlorophyll a fluorescence, Nicotiana tabacum L., mesophyll surface area, leaf movements.

Sign in / Sign up

Export Citation Format

Share Document