Modified Hematoxylin and Eosin Staining Method for Epoxy-Embedded Tissue Sections

1989 ◽  
Vol 184 (6) ◽  
pp. 635-638 ◽  
Author(s):  
K.A. Pasyk ◽  
C.A. Hassett
Keyword(s):  
Staining Method ◽  
Tissue Sections ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

scholarly journals INFILTRATING DUCTAL CARCINOMA OF MAMMARY GLAND IN A GERMAN SHEPHERD DOG

2012 ◽  
Vol 3 (2) ◽  
pp. 174-176
Author(s):  
R Sharmin ◽  
MM Rahman ◽  
M Masuduzzaman
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Staining Method ◽  
Ductal Carcinoma ◽  
Histopathological Study ◽  
German Shepherd ◽  
German Shepherd Dog ◽  
Infiltrating Ductal Carcinoma ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

A German Shepherd bitch of about 2.5 years old showed swollen ulcerated L-5 mammary gland. The affected L-5 gland and adjacent lymphnode were excised and processed for histopathological study with routine Hematoxylin and Eosin staining method. The ductular epithelial cells showed polymorphism and adenoid pattern growth of cells with hyperchromatric nuclei. Metastasis was not evident in the excised lymphnode. The neoplastic epithelial cells infiltrated the surround tissue. On the basis of histopathological finding this case was diagnosed as infiltrating ductal carcinoma.

scholarly journals Cytologic Features of Ventricular Tumors of the Central Nervous System: A Review with Emphasis on Diff-Quik Stained Smears

2021 ◽  
pp. 1-12
Author(s):  
Jose Antonio Jimenez-Heffernan ◽  
Federico Alvarez ◽  
Patricia Muñoz-Hernández ◽  
Carmen Bárcena ◽  
Daniel Azorin ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Staining Method ◽  
Ventricular System ◽  
Primary Tumors ◽  
Common Location ◽  
The Central Nervous System ◽  
Ventricular Tumors ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

<b><i>Background:</i></b> Neoplasms from the ventricular system share a common location but have highly variable histogenesis. Many are slowly growing tumors that behave in a benign fashion. They can be classified as primary and secondary tumors. The most common primary tumors are ependymomas, subependymomas, subependymal giant cell astrocytomas, central neurocytomas, choroid plexus tumors, meningiomas, germinomas, pineal parenchymal tumors, papillary tumors of the pineal region, chordoid gliomas, rosette-forming glioneuronal tumors of the fourth ventricle, and craniopharyngiomas. Pilocytic astrocytomas, medulloblastomas, and atypical teratoid/rhabdoid tumors often show secondary involvement of the ventricular system. <b><i>Summary:</i></b> Advances in neurosurgery have facilitated access to the ventricular system increasing the number of cases in which such tumors can be biopsied. In this context, cytology has been proven to be an extremely useful diagnostic tool during intraoperative pathologic consultations. Many ventricular tumors are infrequent, and the cytologic information available is limited. In this review, we describe the cytologic features of the uncommon ventricular tumors and report on unusual findings of the more common ones. For the cytologic evaluation of brain tumors, many neuropathologists prefer formalin fixation and hematoxylin and eosin staining. In this review, we highlight the cytologic findings as seen with Diff-Quik, a very popular staining method among cytopathologists. In fact, when pathologists are unfamiliar with cytology, it is common to request the assistance of cytopathologists during the evaluation of intraoperative procedures. <b><i>Key Message:</i></b> Ventricular tumors of the central nervous system comprise a group of heterogeneous tumors with very different cytologic features. The cytomorphology of these tumors, including rare entities, is often very characteristic, allowing a precise recognition during intraoperative pathologic consultations. Diff-Quik is a valuable staining method that can be used alone or as a complement to hematoxylin and eosin staining. Diff-Quik allows for clear visualization of the overall architecture, cytoplasmic details, and extracellular material.

scholarly journals A comparative study to evaluate liquid dish washing soap as an alternative to xylene and alcohol in deparaffinization and hematoxylin and eosin staining

2014 ◽  
Vol 6 (02) ◽  
pp. 084-090 ◽  
Author(s):  
Pinki Pandey ◽  
Alok Dixit ◽  
Aparna Tanwar ◽  
Anuradha Sharma ◽  
Sanjeev Mittal
Keyword(s):  
Staining Method ◽  
The Other ◽  
Staining Procedure ◽  
Significant Difference ◽  
Efficient Alternative ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
The Difference ◽  
Different Tissues

ABSTRACT Introduction: Our study presents a new deparaffinizing and hematoxylin and eosin (H and E) staining method that involves the use of easily available, nontoxic and eco-friendly liquid diluted dish washing soap (DWS) by completely eliminating expensive and hazardous xylene and alcohol from deparaffinizing and rehydration prior to staining, staining and from dehydration prior to mounting. The aim was to evaluate and compare the quality of liquid DWS treated xylene and alcohol free (XAF) sections with that of the conventional H and E sections. Materials and Methods: A total of 100 paraffin embedded tissue blocks from different tissues were included. From each tissue block, one section was stained with conventional H and E (normal sections) and the other with XAF H and E (soapy sections) staining method. Slides were scored using five parameters: Nuclear, cytoplasmic, clarity, uniformity, and crispness of staining. Z-test was used for statistical analysis. Results: Soapy sections scored better for cytoplasmic (90%) and crisp staining (95%) with a statistically significant difference. Whereas for uniformity of staining, normal sections (88%) scored over soapy sections (72%) (Z = 2.82, P < 0.05). For nuclear (90%) and clarity of staining (90%) total scored favored soapy sections, but the difference was not statistically significant. About 84% normal sections stained adequately for diagnosis when compared with 86% in soapy sections (Z = 0.396, P > 0.05). Conclusion: Liquid DWS is a safe and efficient alternative to xylene and alcohol in deparaffinization and routine H and E staining procedure. We are documenting this project that can be used as a model for other histology laboratories.

Comparative Tissue Stainability of Lawsonia inermis (Henna) and Eosin as Counterstains to Hematoxylin in Brain Tissues

2015 ◽  
Vol 21 (2) ◽  
pp. 343-350 ◽  
Author(s):  
Judith N. Alawa ◽  
Gbenga O. Gideon ◽  
Bamidele Adetiba ◽  
Clement B. Alawa
Keyword(s):  
Brain Tissue ◽  
Cortical Layer ◽  
Cellular Distribution ◽  
Amino Groups ◽  
Lawsonia Inermis ◽  
Tissue Sections ◽  
Staining Techniques ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
The Brain

AbstractWe hyposthesized that henna staining could provide an alternative to eosin when used as a counterstain to hematoxylin for understanding basic neurohistological principles. Therefore, this study was aimed at investigating the suitability of henna as counterstain to hematoxylin for the demonstration of the layer stratification and cellular distribution in the brain tissue. Henna stained nervous tissue by reacting with the basic elements in proteins via its amino groups. It stained the neuropil and connective tissue membranes brown and effectively outlined the perikarya of neurons with no visible nuclei demonstrating that it is an acidic dye. Henna as a counterstain to hematoxylin demonstrated reliability as a new neurohistological stain. It facilitated identification of cortical layer stratification and cellular distribution in brain tissue sections from Wistar rats. This was comparable to standard hematoxylin and eosin staining as morphological and morphometrical analyses of stained cells did not show significant differences in size or number. This study presents a method for staining with henna and demonstrates that although henna and eosin belong to different dye groups (anthraquinone and xanthenes, respectively) based on their chromophores, they share similar staining techniques and thus could be used interchangeably in neurohistology.

Manual Hematoxylin and Eosin Staining of Mouse Tissue Sections

2014 ◽  
Vol 2014 (6) ◽  
pp. pdb.prot073411-pdb.prot073411 ◽  
Author(s):  
R. D. Cardiff ◽  
C. H. Miller ◽  
R. J. Munn
Keyword(s):  
Mouse Tissue ◽  
Tissue Sections ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

Presence and significance of NK cells in glioblastomas

1989 ◽  
Vol 70 (5) ◽  
pp. 728-731 ◽  
Author(s):  
Jesús Vaquero ◽  
Santiago Coca ◽  
Santiago Oya ◽  
Roberto Martínez ◽  
Josefa Ramiro ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Nk Cells ◽  
Survival Time ◽  
Tumor Cells ◽  
Lymphocytic Infiltration ◽  
Surface Marker ◽  
Content Type ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
Fine Print

✓ A monoclonal antibody against the surface marker IOT-10 of natural killer (NK) cells was used to investigate the presence of these cells in a series of 25 glioblastomas. In 40% of the tumors, IOT-10-positive NK cells were found in small numbers scattered among the tumor cells. The presence of IOT-10-positive NK cells was not related to the degree of lymphocytic infiltration in the tumor as demonstrated by hematoxylin and eosin staining, nor did it appear to influence the survival time of the patients studied.

scholarly journals Complement C9 expression is associated with damaged myocardial cells in pediatric sudden death cases of fulminant myocarditis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Akari Takaya Uno ◽  
Masahito Hitosugi ◽  
Mami Nakamura ◽  
Tomoyuki Nakanishi ◽  
Takahiro Mima ◽  
...  
Keyword(s):  
Sudden Death ◽  
Acute Myocarditis ◽  
Lymphocytic Infiltration ◽  
Pericardial Fluid ◽  
Sirtuin 1 ◽  
Fulminant Myocarditis ◽  
Myocardial Cells ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
Complement C9

Abstract Background Because disease progression is so fast in sudden death of acute fulminant myocarditis, damage of myocardial cells is not evident in routine hematoxylin and eosin staining. To understand damage to myocardial cells and the mechanism of sudden death, immunohistochemical staining was performed for two forensic autopsy cases. Case presentation The patients were a healthy 5-year-old girl and 8-year-old boy. They suddenly died within 2 days of appearance of flu-like symptoms. An autopsy showed accumulation of yellowish-clear pericardial fluid containing fibrin deposits, fluid blood in the heart, and congestion of visceral organs. Histologically, minor necrosis or degeneration of myocardial cells with mainly lymphocytic infiltration was observed sometimes in tissue sections. Immunohistochemically, positive complement C9 staining and negative sirtuin 1 staining were found. These findings suggested wide damage of myocardial cells, even in regions with no marked changes in myocardial cells with hematoxylin and eosin staining. These areas corresponded to those with strong accumulation of lymphocytes. Conclusions Immunohistochemistry for complement C9 and sirtuin 1 might become a new tool for evaluating damage of myocardial cells of fulminant acute myocarditis.

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