scholarly journals INFILTRATING DUCTAL CARCINOMA OF MAMMARY GLAND IN A GERMAN SHEPHERD DOG

2012 ◽  
Vol 3 (2) ◽  
pp. 174-176
Author(s):  
R Sharmin ◽  
MM Rahman ◽  
M Masuduzzaman
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Staining Method ◽  
Ductal Carcinoma ◽  
Histopathological Study ◽  
German Shepherd ◽  
German Shepherd Dog ◽  
Infiltrating Ductal Carcinoma ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

A German Shepherd bitch of about 2.5 years old showed swollen ulcerated L-5 mammary gland. The affected L-5 gland and adjacent lymphnode were excised and processed for histopathological study with routine Hematoxylin and Eosin staining method. The ductular epithelial cells showed polymorphism and adenoid pattern growth of cells with hyperchromatric nuclei. Metastasis was not evident in the excised lymphnode. The neoplastic epithelial cells infiltrated the surround tissue. On the basis of histopathological finding this case was diagnosed as infiltrating ductal carcinoma.

scholarly journals STUDIES ON INFLUENZA INFECTION IN FERRETS BY MEANS OF FLUORESCEIN-LABELLED ANTIBODY

1955 ◽  
Vol 101 (6) ◽  
pp. 665-676 ◽  
Author(s):  
Ch'ien Liu
Keyword(s):  
Epithelial Cells ◽  
Influenza Infection ◽  
Bronchial Epithelium ◽  
Mediastinal Lymph Nodes ◽  
Viral Antigens ◽  
Antibody Staining ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
Ciliated Epithelial Cells ◽  
Infectivity Titer

By means of fluorescein-labelled antibody staining, specific influenza viral antigens were seen in both the cytoplasm and the nucleus of infected ciliated epithelial cells covering the nasal turbinates of infected ferrets. Initially, only a small portion of the nasal epithelium showed fluorescence, and no appreciable abnormality of the cells could be detected by hematoxylin and eosin staining. The fluorescence soon spread to involve the entire epithelium, followed by desquamation coinciding with the onset of manifest illness. Pneumonia was seen in some of the infected ferrets, and in them, viral antigens were found in the bronchial epithelium and in the mediastinal lymph nodes. A rise of viral infectivity titer paralleled the observed spread of viral antigens. Many desquamated nasal epithelial cells and macrophages containing antigen were present in nasal smears. The finding would seem to offer a method for the rapid specific diagnosis of influenza infection.

scholarly journals Cytologic Features of Ventricular Tumors of the Central Nervous System: A Review with Emphasis on Diff-Quik Stained Smears

2021 ◽  
pp. 1-12
Author(s):  
Jose Antonio Jimenez-Heffernan ◽  
Federico Alvarez ◽  
Patricia Muñoz-Hernández ◽  
Carmen Bárcena ◽  
Daniel Azorin ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Staining Method ◽  
Ventricular System ◽  
Primary Tumors ◽  
Common Location ◽  
The Central Nervous System ◽  
Ventricular Tumors ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining

<b><i>Background:</i></b> Neoplasms from the ventricular system share a common location but have highly variable histogenesis. Many are slowly growing tumors that behave in a benign fashion. They can be classified as primary and secondary tumors. The most common primary tumors are ependymomas, subependymomas, subependymal giant cell astrocytomas, central neurocytomas, choroid plexus tumors, meningiomas, germinomas, pineal parenchymal tumors, papillary tumors of the pineal region, chordoid gliomas, rosette-forming glioneuronal tumors of the fourth ventricle, and craniopharyngiomas. Pilocytic astrocytomas, medulloblastomas, and atypical teratoid/rhabdoid tumors often show secondary involvement of the ventricular system. <b><i>Summary:</i></b> Advances in neurosurgery have facilitated access to the ventricular system increasing the number of cases in which such tumors can be biopsied. In this context, cytology has been proven to be an extremely useful diagnostic tool during intraoperative pathologic consultations. Many ventricular tumors are infrequent, and the cytologic information available is limited. In this review, we describe the cytologic features of the uncommon ventricular tumors and report on unusual findings of the more common ones. For the cytologic evaluation of brain tumors, many neuropathologists prefer formalin fixation and hematoxylin and eosin staining. In this review, we highlight the cytologic findings as seen with Diff-Quik, a very popular staining method among cytopathologists. In fact, when pathologists are unfamiliar with cytology, it is common to request the assistance of cytopathologists during the evaluation of intraoperative procedures. <b><i>Key Message:</i></b> Ventricular tumors of the central nervous system comprise a group of heterogeneous tumors with very different cytologic features. The cytomorphology of these tumors, including rare entities, is often very characteristic, allowing a precise recognition during intraoperative pathologic consultations. Diff-Quik is a valuable staining method that can be used alone or as a complement to hematoxylin and eosin staining. Diff-Quik allows for clear visualization of the overall architecture, cytoplasmic details, and extracellular material.

scholarly journals A comparative study to evaluate liquid dish washing soap as an alternative to xylene and alcohol in deparaffinization and hematoxylin and eosin staining

2014 ◽  
Vol 6 (02) ◽  
pp. 084-090 ◽  
Author(s):  
Pinki Pandey ◽  
Alok Dixit ◽  
Aparna Tanwar ◽  
Anuradha Sharma ◽  
Sanjeev Mittal
Keyword(s):  
Staining Method ◽  
The Other ◽  
Staining Procedure ◽  
Significant Difference ◽  
Efficient Alternative ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
The Difference ◽  
Different Tissues

ABSTRACT Introduction: Our study presents a new deparaffinizing and hematoxylin and eosin (H and E) staining method that involves the use of easily available, nontoxic and eco-friendly liquid diluted dish washing soap (DWS) by completely eliminating expensive and hazardous xylene and alcohol from deparaffinizing and rehydration prior to staining, staining and from dehydration prior to mounting. The aim was to evaluate and compare the quality of liquid DWS treated xylene and alcohol free (XAF) sections with that of the conventional H and E sections. Materials and Methods: A total of 100 paraffin embedded tissue blocks from different tissues were included. From each tissue block, one section was stained with conventional H and E (normal sections) and the other with XAF H and E (soapy sections) staining method. Slides were scored using five parameters: Nuclear, cytoplasmic, clarity, uniformity, and crispness of staining. Z-test was used for statistical analysis. Results: Soapy sections scored better for cytoplasmic (90%) and crisp staining (95%) with a statistically significant difference. Whereas for uniformity of staining, normal sections (88%) scored over soapy sections (72%) (Z = 2.82, P < 0.05). For nuclear (90%) and clarity of staining (90%) total scored favored soapy sections, but the difference was not statistically significant. About 84% normal sections stained adequately for diagnosis when compared with 86% in soapy sections (Z = 0.396, P > 0.05). Conclusion: Liquid DWS is a safe and efficient alternative to xylene and alcohol in deparaffinization and routine H and E staining procedure. We are documenting this project that can be used as a model for other histology laboratories.

scholarly journals Immunohistopathologic Characterization of a Dermal Melanocytoma-Acanthoma in a German Shepherd Dog

2000 ◽  
Vol 37 (3) ◽  
pp. 268-271 ◽  
Author(s):  
A. Espinosa De los Monteros ◽  
J. Martín de las Mulas ◽  
A. Fernández ◽  
J. Orós ◽  
F. Rodríguez
Keyword(s):  
Epithelial Cells ◽  
Squamous Epithelium ◽  
German Shepherd ◽  
Epithelial Component ◽  
German Shepherd Dog ◽  
S 100 ◽  
Stratified Squamous Epithelium ◽  
Well Differentiated ◽  
Two Populations

A cutaneous melanocytoma-acanthoma in a 2-year-old female German Shepherd Dog was characterized by the presence of two populations of neoplastic cells: epithelial and melanocytic. The epithelial component consisted of nests of well-differentiated stratified squamous epithelium closely associated with neoplastic melanocytes. The epithelial cells immunoreacted with both monoclonal and polyclonal anti-cytokeratin antibodies, and immunoreaction to S-100 protein and vimentin was observed in the melanocytic cells. This rare pigmented skin neoplasm of the dog apparently has a benign behavior.

Presence and significance of NK cells in glioblastomas

1989 ◽  
Vol 70 (5) ◽  
pp. 728-731 ◽  
Author(s):  
Jesús Vaquero ◽  
Santiago Coca ◽  
Santiago Oya ◽  
Roberto Martínez ◽  
Josefa Ramiro ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Nk Cells ◽  
Survival Time ◽  
Tumor Cells ◽  
Lymphocytic Infiltration ◽  
Surface Marker ◽  
Content Type ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
Fine Print

✓ A monoclonal antibody against the surface marker IOT-10 of natural killer (NK) cells was used to investigate the presence of these cells in a series of 25 glioblastomas. In 40% of the tumors, IOT-10-positive NK cells were found in small numbers scattered among the tumor cells. The presence of IOT-10-positive NK cells was not related to the degree of lymphocytic infiltration in the tumor as demonstrated by hematoxylin and eosin staining, nor did it appear to influence the survival time of the patients studied.

scholarly journals Mammary gland adipocytes in lactation cycle, obesity and breast cancer

2021 ◽  
Author(s):  
Georgia Colleluori ◽  
Jessica Perugini ◽  
Giorgio Barbatelli ◽  
Saverio Cinti
Keyword(s):  
Breast Cancer ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Close Association ◽  
Critical Role ◽  
Exocrine Gland ◽  
Plastic Properties ◽  
Lactation Cycle ◽  
Gland Development

AbstractThe mammary gland (MG) is an exocrine gland present in female mammals responsible for the production and secretion of milk during the process of lactation. It is mainly composed by epithelial cells and adipocytes. Among the features that make the MG unique there are 1) its highly plastic properties displayed during pregnancy, lactation and involution (all steps belonging to the lactation cycle) and 2) its requirement to grow in close association with adipocytes which are absolutely necessary to ensure MG’s proper development at puberty and remodeling during the lactation cycle. Although MG adipocytes play such a critical role for the gland development, most of the studies have focused on its epithelial component only, leaving the role of the neighboring adipocytes largely unexplored. In this review we aim to describe evidences regarding MG’s adipocytes role and properties in physiologic conditions (gland development and lactation cycle), obesity and breast cancer, emphasizing the existing gaps in the literature which deserve further investigation.

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