Application of conventional UV, photodiode array (PDA) and fluorescence (FL) detection to analysis of phenolic acids in plant material and pharmaceutical preparations

2001 ◽  
Vol 24 (5-6) ◽  
pp. 1065-1072 ◽  
Author(s):  
Grażyna Zgórka ◽  
Sławomir Kawka
Keyword(s):  
Phenolic Acids ◽  
Plant Material ◽  
Pharmaceutical Preparations ◽  
Photodiode Array

scholarly journals Phytochemical Screening, Phenolic Compounds and Antioxidant Activity of Biomass from Lychnis flos-cuculi L. In Vitro Cultures and Intact Plants

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 206 ◽  
Author(s):  
Michał P. Maliński ◽  
Małgorzata Anna Kikowska ◽  
Agata Soluch ◽  
Mariusz Kowalczyk ◽  
Anna Stochmal ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Plant Material ◽  
Total Phenolics ◽  
In Vitro Cultures ◽  
Antioxidant Potential ◽  
Phytochemical Screening ◽  
Triterpenoid Saponins ◽  
Intact Plants

Lychnis flos-cuculi L., a species with potential medicinal value, contains flavonoids, phenolic acids, triterpenoid saponins and ecdysteroids. In this study, the antioxidant activity of plant material of L. flos-cuculi obtained from in vitro cultures compared to that of intact plants from the natural site has been evaluated for the first time. Phytochemical screening of the in-vitro-derived material by ultra-high-performance liquid chromatography mass spectrometry (UHPLC-MS) confirmed the presence of the aforementioned metabolite classes. The aqueous methanolic extracts from in-vitro-derived plant material and the organs of intact plants were analyzed using spectrophotometric methods to quantify total phenolics, phenolic acids and flavonoids, and determine the preliminary antioxidant activity by ferric reducing antioxidant potential (FRAP) and DPPH radical scavenging activity assays. The results showed that the inflorescence (Ns-F), and flowering herb of both plants gathered from natural habitat (Ns-H) and in-vitro-derived plants from the experimental plot (ExV-H) are the materials richest in polyphenols (195.4, 113.47, 112.1 mg GAE g−1 d.w., respectively), and demonstrate the highest antioxidant activity (20.14, 11.24, and 11.46 mg AAE g−1 d.w.). The extract from callus exhibited the lowest polyphenol content and antioxidant potential. The contents of total phenolics, flavonoids and phenolic acids correlate with the results of the antioxidant capacity of L. flos-cuculi extracts.

Validated Stability-Indicating Methods for the Simultaneous Determination of Amiloride Hydrochloride, Atenolol, and Chlorthalidone Using HPTLC and HPLC with Photodiode Array Detector

2013 ◽  
Vol 96 (2) ◽  
pp. 313-323 ◽  
Author(s):  
Rasha M Youssef ◽  
Hadir M Maher ◽  
Eman I El-Kimary ◽  
Ekram M Hassan ◽  
Magda H Barary
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Pharmaceutical Preparations ◽  
Array Detector ◽  
Photodiode Array Detection ◽  
Stability Indicating ◽  
Chromatographic Methods ◽  
Photodiode Array ◽  
Amiloride Hydrochloride

Abstract Two stability-indicating chromatographic methods are described for simultaneous determination of amiloride hydrochloride (AMI), atenolol (ATE), and chlorthalidone (CHL) in combined dosage forms. The first method was based on HPTLC separation of the three drugs followed by densitometric measurements of their bands at 274 nm. The separation was carried out on Merck HPTLC silica gel 60F254 aluminum sheets using chloroform–methanol–ammonia 27%, w/w (9 + 2 + 0.3, v/v/v) mobile phase. Analysis data was used for the linear regression graph in the range of 0.1–0.5, 0.8–5.0, and 0.3–1.5 μg/band for AMI, ATE, and CHL, respectively. The second method was based on an RP-HPLC separation of the cited drugs performed on an RP stainless steel C18 analytical column (250 × 4.6 mm id) with a gradient elution system of methanol and 0.05 M aqueous phosphate buffer adjusted to pH 4 as the mobile phase, at the flow rate of 1.0 mL/min. Quantitation was achieved with photodiode array detection at 275 nm for AMI and 225 nm for ATE and CHL. The calibration graphs for each drug were rectilinear in the range of 2–50, 25–150, and 2–100 μg/mL for AMI, ATE, and CHL, respectively. The proposed chromatographic methods were successfully applied for determination of the investigated drugs in pharmaceutical preparations. Both methods were validated in compliance with International Conference on Harmonization guidelines in terms of linearity, accuracy, precision, robustness, LOD, and LOQ.

A New Application of PARAFAC Model to UPLC Dataset for the Quantitative Resolution of a Tri-Component Drug Mixture

2021 ◽  
Author(s):  
Remziye Güzel ◽  
Zehra Ceren Ertekin ◽  
Erdal Dinç
Keyword(s):  
Quantitative Estimation ◽  
Pharmaceutical Preparation ◽  
Internal Standard ◽  
Pharmaceutical Preparations ◽  
Ultra Performance Liquid Chromatography ◽  
Parafac Model ◽  
Independent Test ◽  
Photodiode Array ◽  
Parallel Factor ◽  
Parafac Decomposition

Abstract In the presented work, a three-way analysis of ultra-performance liquid chromatography-photodiode array (UPLC-PDA) dataset was performed by parallel factor analysis (PARAFAC) for quantitatively resolving a ternary mixture containing paracetamol and methocarbamol with indapamide selected as an internal standard in their co-eluted chromatographic conditions. Paracetamol and methocarbamol were quantified in the working range between 3–24 and 5–50 μg/mL by applying PARAFAC decomposition to UPLC-PDA data array obtained under unresolved chromatographic peak conditions. To compare the experimental results provided by co-eluted UPLC-PARAFAC method, an ordinary UPLC method was developed ensuring proper separation of the peaks. The performance of both PARAFAC and ordinary UPLC methods were assessed by quantifying independent test samples, intra- and inter-day samples and spiked samples of pharmaceutical preparations. Then, both methods were applied for quantitative estimation of the related drugs in a commercial pharmaceutical preparation. In this study, PARAFAC method was proved to be a very powerful alternative for the quality control of pharmaceutical preparations containing paracetamol and methocarbamol even in their co-eluted chromatograms with high precision and accuracy in a short chromatographic runtime of 1.2 min.

scholarly journals HPLC Characterization and Assessment of Antioxidant Status of Vetiveria Zizanioides Roots

2019 ◽  
Vol 8 (2S10) ◽  
pp. 482-485
Keyword(s):  
Phenolic Acids ◽  
Cell Damage ◽  
Gradient Elution ◽  
Array Detector ◽  
Root Extract ◽  
Enzymatic Antioxidants ◽  
Vetiveria Zizanioides ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Liquid Chromatographic

Vetiveria zizanioides has been assigned for the extraction of phenolic acids and flavonoids for soluble, glycoside and wall-bound fractions. There was the largest number of phenolic acids and flavonoids in the methanolic extract that constitutes the cell wall-bound portion. Free radicals can induce biomolecules to oxidize, resulting in cell damage and countless illnesses. The present study investigates the role of enzymatic antioxidants, i.e. catalase, superoxide dismutase, glutathione peroxidase,glutathione reductase.Vitamin Eand C enzyme activity was nonenzymaticantioxidant action by spectrophotometric method. The enzymatic glutothi-one peroxidase antioxidant was found to be exampling than the rest while Vitamin E, notified found to be best activity ratherthan Vitamin C.The reversed highperformance liquid chromatographic techniquewas created and validated for the concurrent identification of free phenolic acids and flavonoids using a photodiode array detector with gradient elution.(Caffeicacid, Hydroxy benzoic acid, Rutin, Quercetin, Para- cumaric acid and Kaempferol) in the methanolic root extract of Vetiveria zizanioides.

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