Morphological changes in thrombocytes during blood bank storage. An ultrastructural morphometric study

1993 ◽  
Vol 175 (2) ◽  
pp. 163-170 ◽  
Author(s):  
Matthias H.F. Klinger ◽  
Harald Klüter
Keyword(s):  
Morphological Changes ◽  
Blood Bank ◽  
Morphometric Study

Morphometric study of the platform elements of Amydrotaxis praejohnsoni n. sp. (Lower Devonian, conodonts, Nevada)

1989 ◽  
Vol 63 (3) ◽  
pp. 349-355 ◽  
Author(s):  
Michael A. Murphy ◽  
Kathleen B. Springer
Keyword(s):  
Lower Devonian ◽  
Morphological Changes ◽  
Morphometric Study ◽  
Mosaic Pattern ◽  
Basal Cavity

The platform element of the conodont Amydrotaxis praejohnsoni n. sp. shows statistically significant morphological changes from the base to the top of the delta Zone (Lower Devonian). The changes occur particularly in mean denticle number, height and character of the anterior denticles, and shape of the basal cavity. The earliest forms have fewer denticles ( = 7.1), an enlarged, high anterior denticle, and a broadly flared posterior basal cavity, whereas late forms have more denticles ( = 9.4), equal-sized anterior denticles, and a narrow posterior basal cavity. There is no stasis phase in the species history, but rather a mosaic pattern in which the observed changes in the character states are seemingly independent of each other and proceed at variable rates.

Features of morphological changes in the intramural nervous system of the heart in atherosclerosis and myocardial infarction

1980 ◽  
Vol 61 (4) ◽  
pp. 26-28
Author(s):  
M. V. Uglova ◽  
V. N. Shlyapnikov ◽  
V. V. Sergeev ◽  
A. U. Zalmunin ◽  
E. A. Taikov
Keyword(s):  
Myocardial Infarction ◽  
Nervous System ◽  
Morphological Changes ◽  
Morphometric Study ◽  
Hypertrophic Changes

Morphometric study of human cardiac neurocytes in myocardial infarction and atherosclerosis made it possible to establish that myocardial infarction causes adaptive (hypertrophic) changes in the intramural nervous system of the heart, especially pronounced on the first day of the course of the infarction; with atherosclerosis, the changes are characterized by a direction towards atrophic processes.

scholarly journals To Determine the Morphological Changes in Red Blood Cells During Storage in Blood Banks

2020 ◽  
Vol 10 (2) ◽  
Author(s):  
SM Tahir ◽  
Wajid Akbar ◽  
Asadullah . ◽  
Usmanullah . ◽  
Usman Ali ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Light Microscope ◽  
Morphological Changes ◽  
Blood Bank ◽  
Blood Banks ◽  
Abnormal Cells ◽  
The Mean ◽  
Blood Bag ◽  
Stored Blood

Background: During storage of blood, the red blood cells undergo shape changes which cause fragility and endothelial interaction leading to deterioration the quality of blood in blood banks.Objectives: The aim of this study is to determine the morphological changes in red blood cells during storage in blood banks. Material and Methods: In this experimental study, a total 20 healthy volunteers between 17 to 40 years blood donors-Blood bags were taken, ten from each center i.e. MMCTH blood bank Mardan and KTH blood bank Peshawar. The specimen analysis was done at IBMS (Institute of Basic Medical Sciences) of KMU (Khyber Medical University) Peshawar. The exclusion criteria were People with anemia, hepatitis B &C, HIV and syphilis. The duration of this study was six months. The inform consent was taken from each donor. The total blood 250 ml from vein in cubital fossa from each blood donor was collected in 250ml pediatric blood bag with CPDA-1 solution. Blood bags were put up in the blood bank at +2 to +6 °C and stored till 20 days. Blood specimen of about 5cc were collected in 5cc syringe from each blood bag on 0, 5th,10th ,15th and 20th day for following parameters and thin film red blood cell was prepared for examination by light microscope. Morphological changes in RBCs examined via light microscope as well as grading the RBCs status in the peripheral blood film, the occurrence of distorted RBC simply in random fields; such as +1(scored 1 to 5 altered RBC present in each field), +2 (an average of 6 to 15 altered RBC in each field), +3(16 to 25 altered RBC in each field) and +4(more than 25 altered RBC present in each field). The multi head light microscope NIKON eclipse 50 was used for examination of peripheral blood slide and we took images of randomly selected field. The image J software was used for slide examination.Results: The morphological analysis of red blood cells, count of 200 cells in each blood slide in randomly selected fields are: On day 0 the majority of cells were normally shaped (97.95±1.297 (mean±SD).With increasing storage time, the percentage of morphologically abnormal red cells rose sharply. Mean percentage of abnormal cells on day 5, 10, 15 and 20 was 28.80±10.00, 51.73±12.47, 64.78±14.66 and 68.10±7.92 respectively. This increase in percentage of abnormally shaped cells was significant as determined by one way ANOVA (p =0.001). There was a big difference of percentage of abnormal RBCs on day 0 and in = 5 to= 10 days and in = 15 to = 20 days of blood storage. The mean values of day 0 of abnormal cells was 2.05±1.297 (Mean ± Std. Deviation), abnormal cells in= 5 to= 10 days was 40.26± 16.101 (Mean ± Std. Deviation) and on day = 15 and in = 20 day was 66.44± 11.75. The mean difference from day 0 to day 20 was 63.93±10.45 (Mean ± Std. Deviation).The one way ANOVA was significant, P= 0.001.Conclusion: This study confirms the hematological and morphological changes, when blood stored at 2 °C to 6 °C for up to 21 days. The significant morphological changes were observed on 5th day of blood storage. These findings suggested that approximately a week old stored blood is as good as the fresh blood; however, significant morphological and biochemical changes begin to appear after the first week of storage and these changes aggravate with time. Hence in order to achieve best possible transfusion outcomes, stored blood up to one week can be utilized.

scholarly journals MORPHOLOGICAL CHANGES IN THE LIVER AT DIFFERENT INVASIVE DOSES OF OPHISTORCHIS FELINEUS (RIVOLTA, 1884) IN RODENTS (ORYCTOLAGUS CUNICULUS)

2021 ◽  
pp. 484-488
Author(s):  
Sidelnikova
Keyword(s):  
Connective Tissue ◽  
Oryctolagus Cuniculus ◽  
Morphological Changes ◽  
Intermediate Stage ◽  
Morphometric Study ◽  
Comparative Aspect ◽  
Dead Fish ◽  
Liver Changes ◽  
Leuciscus Idus ◽  
Portal Tracts

A morphometric study of liver changes at different invasive doses was conducted in rodents (Oryctolagus cuniculus). In a comparative aspect, the thickness of connective tissue formed around the portal tracts and the thickness of cellular infiltrates in the same area were studied at an invasive dose of 100, 50 and 10 metacercariae of Ophistorchis felineus (Rivolta, 1884). The experiment was conducted on sexually mature male rabbits at the age of 6 months, in each observation group of 10 individuals. Clinically healthy animals were infested with Ophistorchis felineus per os metacercariae once. The intermediate stage of the parasite was isolated from a dead fish (ide, Leuciscus idus (Linnaeus, 1758) and dace fish, Leuciscus leuciscus) obtained from the Tom River in Tomsk. The invasion that took place after 1 month was confirmed by a positive analysis of feces for parasite eggs by the Ragaser and KatoMiura methods. The animals were sacrificed after 5 weeks from the period of infestation. Histological preparations of the liver were made, stained with hematoxylin and eosin, according to Van Gieson. Histological preparations were studied by light microscopy with morphometry. The results obtained characterize a twofold change in the morphometric parameters of connective tissue thickness and cellular infiltration around the portal tracts of the liver lobes with an increase in the invasive dose.

Comparing the effects of surgical treatment and chemotherapy on the thymus structure in experimental breast cancer

2021 ◽  
pp. 37-43
Author(s):  
О.В. Казаков ◽  
А.Ф. Повещенко ◽  
А.В. Кабаков ◽  
О.В. Повещенко ◽  
Д.Н. Стрункин ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Surgical Treatment ◽  
Connective Tissue ◽  
Structural Changes ◽  
Morphological Changes ◽  
Morphometric Study ◽  
Epithelial Tumors ◽  
Thymus Involution ◽  
Cortical Substance ◽  
Number Of Cells

Цель исследования - выявление структурных преобразований тимуса самок крыс Вистар при хирургическом лечении рака молочной железы и при химиотерапии. Методика. Рак молочной железы (РМЖ) индуцировали введением N-метил-N-нитрозомочевины 5 раз с интервалом 7 сут подкожно в область 2-й молочной железы справа. Оперативное лечение РМЖ проводили через 6 мес от момента индукции РМЖ. Курс химиотерапии проводили по схеме CMF. Гистологическое исследование тимуса и опухоли молочной железы проводили по стандартной методике. Методом точечного счёта производили морфометрию срезов и подсчет клеточных элементов в опухоли и структурно-функциональных зонах тимуса. Результаты. При РМЖ без лечения выявлены структурные признаки акцидентальной инволюции тимуса. После оперативного лечения РМЖ акцидентальная инволюция тимуса менее выражена по сравнению с химиотерапией. После оперативного лечения РМЖ, как и в группе РМЖ без лечения, в тимусе сохраняется уменьшение площади коркового вещества. По сравнению с РМЖ без лечения в тимусе уменьшалась площадь железистых эпителиальных образований, увеличилась плотность расположения клеточных элементов в корковом веществе. В структурных зонах тимуса увеличивалось количество макрофагов, снижалось количество клеток с пикнотичными ядрами. После химиотерапии, по сравнению с резекцией опухоли МЖ, в тимусе выявлены структурные признаки прогрессирования процесса инволюции. В тимусе уменьшена площадь соединительно-тканных элементов, снижено количество митозов в субкапсулярной зоне коркового вещества. Во всех исследуемых зонах увеличено количество клеток с пикнотичными ядрами и уменьшено число макрофагов. Заключение. После оперативного лечения РМЖ акцидентальная инволюция тимуса проявляется в менее выраженной форме, чем в группе с РМЖ без лечения. После химиотерапии РМЖ, по сравнению с оперативным лечением РМЖ, морфологические перестройки в тимусе свидетельствуют о снижении активности лимфоидного и эпителиального компонентов и увеличении числа гибнущих клеток. Aim. To identify structural changes in the thymus of female Wistar rats during surgical treatment of breast cancer or with chemotherapy. Methods. Breast cancer (BC) was induced by subcutaneous injection of N-methyl-N-nitrosourea into the second right breast 5 times at 7 day intervals. Resection of BC was performed 6 mos after BC induction, or chemotherapy was administered according to the CMF scheme. Histological examination of the thymus and breast tumor was performed according to a standard method. Morphometric study of sections and counting cell elements in the tumor and in structural and functional areas of the thymus were performed by point counting. Results. In untreated BC, structural signs of accidental thymus involution were evident. There was an increase in the area of connective tissue elements and of glandular epithelial tumors. After surgical treatment of BC, accidental thymus involution was less pronounced than after chemotherapy. Also after surgical treatment, as in the case of untreated BC, the thymus cortical substance remained decreased. Compared with untreated BC, the area of glandular epithelial tumors in the thymus decreased, and the density of cellular elements in the cortical substance increased. In the structural zones of the thymus, the number of macrophages increased, and the number of cells with pyknotic nuclei decreased. After chemotherapy, in comparison with surgical treatment, structural signs of involution of the thymus were evident. In the thymus, the area of connective tissue elements was reduced, and the number of mitotically dividing cells in the subcapsular zone of the cortical substance was reduced. In all the zones of the thymus, the number of cells with pyknotic nuclei increased, and the number of macrophages decreased. Conclusion. After surgical treatment of BC, accidental thymus involution was less pronounced than in the untreated group. After chemotherapy, compared with surgical treatment of BC, morphological changes in the thymus may indicate a decrease in the activity of the lymphoid and epithelial components and an increase in the number of dying cells.

Use of Vitamin K Antagonists and Brain Morphological Changes in Older Adults: An Exposed/Unexposed Voxel-Based Morphometric Study

2018 ◽  
Vol 45 (1-2) ◽  
pp. 18-26 ◽  
Author(s):  
Antoine Brangier ◽  
Sébastien Celle ◽  
Frédéric Roche ◽  
Olivier Beauchet ◽  
Guylaine Ferland ◽  
...  
Keyword(s):  
Older Adults ◽  
White Matter ◽  
Vitamin K ◽  
Morphological Changes ◽  
Vitamin K Antagonists ◽  
Statistical Parametric Mapping ◽  
Morphometric Study ◽  
Intracranial Volume ◽  
Brain Physiology ◽  
Vitamin K Cycle

Background: Vitamin K antagonists (VKAs) are commonly used for their role in haemostasis by interfering with the vitamin K cycle. Since vitamin K also participates in brain physiology, this voxel-based morphometric study aimed to determine whether the duration of exposure to VKAs correlated with focal brain volume reduction in older adults. Methods: In this exposed/unexposed (1: 2) study nested within the GAIT (Gait and Alzheimer Interactions Tracking) cohort, 18 participants exposed to VKA (mean age 75 ± 5 years; 33.3% female; mean exposure 2,122 ± 1,799 days) and 36 matched participants using no VKA (mean age 75 ± 5 years; 33.3% female) underwent MRI scanning of the brain. Cortical grey and white matter volumes were automatically segmented using statistical parametric mapping. Age, gender, educational level, history of atrial fibrillation, type of MRI, and total intracranial volume were included as covariables. Results: The duration of exposure to VKA correlated inversely across the whole brain with the subvolumes of two clusters in the grey matter (right frontal inferior operculum and right precuneus) and one cluster in the white matter (left middle frontal gyrus). In contrast, the grade of white matter hyperintensities did not differ according to the use of VKA. Conclusion: We found focal atrophies in older adults exposed to VKA. These findings provide new insights elucidating the effects of VKAs on brain health and function in older adults.

scholarly journals Morphological changes of isolated rat pancreatic islets: a structural, ultrastructural and morphometric study

2006 ◽  
Vol 209 (3) ◽  
pp. 381-392 ◽  
Author(s):  
Sergio Morini ◽  
Mark Braun ◽  
Paolo Onori ◽  
Luca Cicalese ◽  
George Elias ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Morphological Changes ◽  
Morphometric Study ◽  
Rat Pancreatic Islets ◽  
Isolated Rat

scholarly journals Morphometric analysis of the masseter muscle post unilateral lower molar exodonty: experimental study

2016 ◽  
Vol 33 (02) ◽  
pp. 090-095
Author(s):  
M. Benigno ◽  
E. Amstalden ◽  
E. Liberti ◽  
N. Leal ◽  
K. Rodrigues ◽  
...  
Keyword(s):  
Morphometric Analysis ◽  
Masseter Muscle ◽  
Wistar Rats ◽  
Morphological Changes ◽  
Optical Microscope ◽  
Morphometric Study ◽  
Surgical Extraction ◽  
Molar Teeth ◽  
Masseter Muscles ◽  
Lower Molar

Abstract Introduction: To check the morphological changes of the masseter muscle after surgical extraction of inferior molar teeth. Methods: 24 Wistar rats had samples of their masseter muscles analysed in the Optical Microscope (OM). The rats were randomly divided into three groups as follows: GI-15, GII-30, GII-60 days after surgical extraction of inferior molar teeth. Each group had 5 experimental and 3 normal control rats for OM observation. The OM allowed the morphometric study of the masseter muscle. The morphometric study was based on the measurement of the fiber's area by AXION-VISION software. ANOVA test was applied for data analysis. Results: No alteration was detected in all morphometric analysis of the masseter muscle. Conclusion: The masseter muscle adapts to the occlusal modiication caused by the extraction of the molar teeth during the analysed periods.

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