Morphometric analysis of the masseter muscle post unilateral lower molar exodonty: experimental study

Abstract Introduction: To check the morphological changes of the masseter muscle after surgical extraction of inferior molar teeth. Methods: 24 Wistar rats had samples of their masseter muscles analysed in the Optical Microscope (OM). The rats were randomly divided into three groups as follows: GI-15, GII-30, GII-60 days after surgical extraction of inferior molar teeth. Each group had 5 experimental and 3 normal control rats for OM observation. The OM allowed the morphometric study of the masseter muscle. The morphometric study was based on the measurement of the fiber's area by AXION-VISION software. ANOVA test was applied for data analysis. Results: No alteration was detected in all morphometric analysis of the masseter muscle. Conclusion: The masseter muscle adapts to the occlusal modiication caused by the extraction of the molar teeth during the analysed periods.

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Histological, Histochemical, and Protein Changes after Induced Malocclusion by Occlusion Alteration of Wistar Rats

BioMed Research International ◽

10.1155/2014/563463 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Carolina de Souza Guerra ◽

Yamba Carla Lara Pereira ◽

João Paulo Mardegan Issa ◽

Kelly Galisteu Luiz ◽

Elaine A. Del Bel Guimarães ◽

...

Keyword(s):

Masseter Muscle ◽

Wistar Rats ◽

Morphological Analysis ◽

Contralateral Side ◽

Environmental Interventions ◽

Stomatognathic System ◽

Masseter Muscles ◽

Experimental Group ◽

Muscle Remodeling

Although disorders of the stomatognathic system are common, the mechanisms involved are unknown. Our objective was to study the changes in the masseter muscles after unilateral exodontia. Molar extraction was performed on Wistar rats (left side), and the animals were sacrificed after either 14 or 26 days. The masseter muscle was processed for histological analysis, conventional andin situzymography, and immunohistochemistry. The morphological analysis showed unique and specific characteristics for the experimental group. By conventional zymography no significant values of 72 kDa MMP-2 (P< 0.05) were found in both of the sides of masseter muscle after 14 and 26 days of unilateral extraction. Thein situzymography showed gelatinolytic activity on all deep masseter muscles, with significant increase on the contralateral side after 14 and 26 days (P< 0.05). The immunohistochemistry demonstrated greater expression of MMP-2 than MMP-9 and MMP-14 in all masseter muscles and there were few differences in the staining of 4 TIMPs. This knowledge about morphology and molecular masticatory muscle remodeling following environmental interventions can be used to develop clinically successful treatments.

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INFLUENCE OF INDUSTRIAL VIBRATION ON MINERAL METABOLISM, ORGANS OF THE ORAL CAVITY AND FETAL MYOCARDIUM

Sibirskij medicinskij vestnik ◽

10.31549/2541-8289-2021-1-22-28 ◽

2021 ◽

pp. 22-28

Author(s):

S.V. Zalavina ◽

◽

Е.Yu. Apraksina ◽

Keyword(s):

Masseter Muscle ◽

Wistar Rats ◽

Mineral Metabolism ◽

Morphometric Study ◽

Interstitial Edema ◽

Cardiovascular Pathology ◽

Pregnant Females ◽

Bone Trabeculae ◽

Tooth Buds ◽

Prenatal Ontogenesis

In the study, the morphometric analysis of bone trabeculae of the developing jaws, tooth buds, tongue muscles, masseter muscle, and myocardium of 20-day fetuses of Wistar rats, exposed to vibration from the 9th to the 18th day of prenatal ontogenesis, was performed. The concentration of Ca, Cd, Cu, Fe, Mg, P, Pb, Se, Zn was determined in the liver of pregnant females exposed to vibration. The morphometric study revealed an acceleration of the osteogenesis of jaws and dentinogenesis of tooth buds. In the myocardium and masseter muscle signs of interstitial edema and a decrease in the area of the vessels of the microvasculature are determined. In the liver, there is a decrease in the amount of Ca, Mg and Fe, which is accompanied by an increase in the content of Cd and Pb. The revealed shifts in mineral metabolism indicate gross impairment of chemicals' homeostasis in the mother-fetus system, which underlies the imperfect morphogenesis of the fetal dentition and will be the basis for the formation of pathology of orofacial organs and cardiovascular pathology in offspring.

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Masseter muscle surface electromyography in college students with a high degree of anxiety and temporomandibular disorder

Revista CEFAC ◽

10.1590/1982-021620182017617 ◽

2018 ◽

Vol 20 (1) ◽

pp. 44-52 ◽

Cited By ~ 1

Author(s):

Eduarda de Lima Amarante ◽

Jully Anne Soares de Lima ◽

Rafael Nóbrega Bandeira ◽

Ana Paula Arruda de Moura ◽

Luciane Spinelli de Figueiredo Pessoa ◽

...

Keyword(s):

College Students ◽

Electrical Activity ◽

Masseter Muscle ◽

Speech Therapy ◽

Temporomandibular Disorder ◽

P Value ◽

Voluntary Activity ◽

Group 2 ◽

High Degree ◽

Masseter Muscles

ABSTRACT Objective: to compare the electrical activity of masseter muscles, bilaterally, according to the presence or absence of Temporomandibular Disorder (TMD) in college students with a high degree of anxiety. Methods: the study was conducted with a randomized sample of 31 Speech Therapy students aged between 17 and 32 years; 61.3% (n = 19) were females and 38.7% (n = 12) were males. They were divided into two groups, Group 1 (G1), comprising 11 students with TMD, and Group 2 (G2), composed of 20 students without TMD. The college students answered the State-Trait Anxiety Inventory (STAI) for anxiety investigation, and were evaluated by the protocol Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) for TMD diagnosis. The evaluation of muscular electrical activity took into account the records in the conditions of rest, Sustained Maximum Voluntary Activity (SMVA) and habitual chewing (HC). The data were analyzed using the version 22 IBM Statistical Package for Social Sciences (SPSS) software. The statistical analysis was performed using Student t test to compare means between groups, considering < 0,05 as the significant p-value. Results: college students, of both groups, presented high levels of anxiety traits. Significant statistical differences were observed on the percentage of electrical activity of right masseter muscle in chewing function, as well as muscle fibers recruitment during chewing, which were higher on the group without TMD. Conclusion: college students with TMD and a high degree of anxiety presented lower means of masseter muscle electromyografic activity during chewing, in most conditions assessed, as compared to volunteers without TMD, except for the left masseter muscle in rest and chewing.

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Comparison of histometric and morphometric analyses of bone height in ligature-induced periodontitis in rats

Brazilian Oral Research ◽

10.1590/s1806-83242007000300005 ◽

2007 ◽

Vol 21 (3) ◽

pp. 216-221 ◽

Cited By ~ 31

Author(s):

Marilene Issa Fernandes ◽

Eduardo José Gaio ◽

Rui Vicente Oppermann ◽

Pantelis Varvaki Rados ◽

Cassiano Kuchenbecker Rosing

Keyword(s):

Periodontal Disease ◽

Morphometric Analysis ◽

Wistar Rats ◽

T Test ◽

The Other ◽

Limited Capacity ◽

Height Measurement ◽

Morphometric Analyses ◽

Left And Right ◽

Bone Height

The purpose of this study was to compare histologic and morphometric procedures of bone height measurement. Microscopic measurements are the most frequent methods in periodontal studies with animals, but have limited capacity to identify bone levels associated with both healthy tissues and periodontal disease. Ligatures were placed in the maxillary left second molars of 10 male 60-day-old Wistar rats for 30 days. Left and right maxillary sides of 5 rats were processed for histologic analysis (H), sectioned buccolingually, and stained with HE. The maxillae of the other 5 rats were defleshed and used for morphometric analysis (M). Histometric measurements from the cementoenamel junction to the bone crest were performed. Standardized photographs were used for morphometric analysis. The t test was used for dependent or independent samples (alpha = 0.05%). Distances from cementoenamel junction to bone crest were 0.95 ± 0.25 and 1.07 ± 0.30 mm for H and M, respectively. Buccal measurements were 0.92 ± 0.16 and 1.08 ± 0.35 mm for H and M. The values obtained using H and M for areas without ligatures were 0.44 ± 0.15 and 0.47 ± 0.11 mm for lingual measurements and 0.23 ± 0.08 and 0.41 ± 0.10 mm for buccal measurements. No significant differences were found between the two methods in the detection of bone height associated with the placement of ligatures in rats.

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Localization of DNA in the fibrillar components of the nucleolus: a cytochemical and morphometric study.

Journal of Histochemistry & Cytochemistry ◽

10.1177/41.6.8315275 ◽

1993 ◽

Vol 41 (6) ◽

pp. 829-836 ◽

Cited By ~ 17

Author(s):

M Derenzini ◽

F Farabegoli ◽

D Trerè

Keyword(s):

Morphometric Analysis ◽

Mean Value ◽

Morphometric Study ◽

Dense Fibrillar Component ◽

Thin Sections ◽

Fibrillar Center ◽

The Mean ◽

Fibrillar Component ◽

Resting Lymphocytes ◽

The Relationship

We studied the distribution of DNA in human circulating lymphocyte nucleoli using three different cytochemical methods for selective visualization of DNA in thin sections: the Feulgen-like osmium-ammine reaction, the NAMA-Ur procedure, and the osmium-ammine staining in glycine buffer, pH 1.5. All three methods indicated the presence of uniformly distributed, highly decondensed DNA filaments forming a large solitary agglomerate in the central part of the nucleolar area, corresponding to the solitary large fibrillar center (FC) as revealed by uranium and lead staining. We also studied the relationship between DNA agglomerates and nucleolar fibrillar components in resting and phytohemagglutinin (PHA)-stimulated lymphocytes by morphometric analysis of the areas occupied by these structures. In resting lymphocytes the mean area of the DNA agglomerates was 0.479 micron 2 +/- 0.161 SD, whereas that of FCs was 0.380 micron 2 +/- 0.149 SD, with a ratio of 1.26. In PHA-stimulated lymphocytes the mean area of the DNA agglomerates was 0.116 micron 2 +/- 0.056 SD, whereas that of the FCs was 0.075 micron 2 +/- 0.032 SD, with a ratio of 1.55. In PHA-stimulated lymphocytes we also measured the area occupied by the FCs plus the closely associated dense fibrillar component (DFC). The mean value of these two fibrillar components was 0.206 micron 2 +/- 0.081 SD. These data demonstrate that decondensed DNA filaments are uniformly distributed in the FCs and that in transcriptionally active nucleoli they are also present in the proximal portion of the DFC surrounding the FCs.

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The Influence of Salt Solution on Morphological Changes in a Geosynthetic Clay Liner

Advances in Materials Science and Engineering ◽

10.1155/2016/6349407 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

Mochamad Arief Budihardjo

Keyword(s):

Salt Solution ◽

Morphological Changes ◽

Microscopic Analysis ◽

Optical Microscope ◽

Distilled Water ◽

Nacl Solution ◽

Geosynthetic Clay Liner ◽

Clay Liner ◽

Morphological Variations ◽

Crystal Deposit

Morphological variations of geosynthetic clay liner (GCL) samples, hydrated with two different permeates, distilled water and NaCl solution (100 mM concentration), were observed in detail using microscopic analysis. After the GCL samples were hydrated with the NaCl solution, they were observed with an optical microscope. While the surface of the treated GCL samples was similar to the surface of the untreated GCL, a crystal deposit was found on the surface of the treated samples. Using a scanning electron microscope (SEM), a more solid appearance was observed for the bentonite particles contained in the GCL after the sample was hydrated with distilled water in comparison to the GCL sample that was hydrated with the NaCl solution. It appears that salt solution hydration results in less swelling of the bentonite particles. Furthermore, the energy-dispersive X-ray spectrometer (EDS) results showed that distilled water hydration had no effect on the distribution of the elements contained in the GCL samples. However, bound chlorine was observed, which demonstrated that the bentonite particles had absorbed the NaCl solution. In addition, changes in the hydraulic conductivity of the hydrated GCL samples were also observed.

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Stiffness of the Masseter Muscle in Children—Establishing the Reference Values in the Pediatric Population Using Shear-Wave Elastography

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18189619 ◽

2021 ◽

Vol 18 (18) ◽

pp. 9619

Author(s):

Cyprian Olchowy ◽

Anna Olchowy ◽

Aleksander Pawluś ◽

Mieszko Więckiewicz ◽

Luca Maria Sconfienza

Keyword(s):

Shear Wave ◽

Masseter Muscle ◽

Pediatric Population ◽

Shear Wave Elastography ◽

Total Sample ◽

Healthy Children ◽

Significant Difference ◽

The Right ◽

The Impact ◽

Masseter Muscles

In children, the quality and muscle function are altered in many pathologic conditions, including temporomandibular disorders. Although several methods have been used to evaluate muscle tonus, none became a golden standard. Moreover, the masseter muscle characteristics in children have not been investigated to date. This study aimed to measure the stiffness of the masseter muscle using shear-wave elastography in healthy children. We enrolled 30 healthy children (mean age 10.87 ± 3.38 years). The stiffness of masseter muscles was measured with shear wave elastography. Stiffness for the total sample was 6.37 ± 0.77 kPa. A comparison of the measurements did not show significant differences between the right and the left masseter muscles (left—6.47 ± 0.78 kPa; right—6.24 ± 0.76 kPa; p = 0.3546). A significant difference was seen between boys and girls (boys—5.94 ± 0.50 kPa; girls—6.63 ± 0.80; p = 0.0006). Shear-wave elastography is a promising diagnostic tool. It may help to detect changes in the stiffness of the masseter muscle and draw attention to pathological processes within the jaw muscles. Directions for further research shall include determining stiffness values in pathological conditions and the impact of biological and functional factors on the stiffness of the masseter muscle.

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Hormones of thyroid gland in sera of rats treated with different dose of concentrated potassium iodine solutions

Srpski arhiv za celokupno lekarstvo ◽

10.2298/sarh1006323m ◽

2010 ◽

Vol 138 (5-6) ◽

pp. 323-327 ◽

Cited By ~ 1

Author(s):

Ljiljana Markovic ◽

Violeta Mihailovic-Vucinic ◽

Jelena Aritonovic

Keyword(s):

Thyroid Gland ◽

Genetic Susceptibility ◽

Experimental Model ◽

Wistar Rats ◽

Morphological Changes ◽

Cell Necrosis ◽

High Iodine ◽

Hormone Status ◽

Potassium Iodine ◽

Iodine Dose

Introduction Potassium iodine (KI) is used as a drug therapy for treating numerous diseases such as small-vessel vasculitis, erythema nodosum, vasculitis nodularis, Sweet's syndrome, tuberculosis and granulomatosis, and for iodized salt. At the same time, KI can be harmful. Iodine intake may increase the frequency of thyroiditis in humans, and may induce the occurrence of experimental thyroiditis (ET) in animals. Investigations on an experimental model for the examination of thyroiditis in Wistar rats have clearly showed morphological changes in the rat thyroid evoked by KI administration. Objective The purpose of this study was to compare the effects of low and high doses of KI on the thyroid gland of Wistar rats and determine the effect on hormone status (T4, T3 and TSH) in this rat strain. Methods Two groups of rats from the Wistar strain were treated with a low iodine dose (225 ?g/g BW) and with a high iodine dose (675 ?g/g BW) of KI solutions. Untreated nonimmunized animals served as controls. The solution was administrated daily intraperitoneally during the period of 26 consecutive days. Results Monitoring hormone status (TSH, T3 and T4) and morphological changes it was found that therapeutic doses of KI applied in treatment induced the occurrence of experimental thyroiditis (chronic destructive Hashimoto's thyroiditis in humans) and cell necrosis in animals not carrying a genetic susceptibility. Significant inflammatory changes were observed in rats treated with a high iodine dose. Conclusion The early iodine induced cell necrosis and inflammation in the nonimmunized animals without genetic susceptibility is a new experimental model of thyroiditis. .

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Morphological alterations in the heart and aorta of rats treated with glucocorticoids

Journal of Morphological Sciences ◽

10.4322/jms.065814 ◽

2015 ◽

Vol 32 (04) ◽

pp. 231-235

Author(s):

R. Dantas ◽

K. Souza ◽

D. Santos ◽

V. Feitosa ◽

E. Fioretto ◽

...

Keyword(s):

Wistar Rats ◽

Histological Analysis ◽

Saline Solution ◽

Morphological Changes ◽

Morphological Structure ◽

Control Group ◽

Morphological Alterations ◽

Heart Chamber ◽

Male Wistar Rats ◽

Synthetic Glucocorticoid

Abstract Introduction: The objective of this study was to analyze the morphological structure of the heart and aorta of rats treated with the synthetic glucocorticoid dexamethasone. Material and Methods: Male Wistar rats were divided into two groups: 08 control rats undergoing treatment with a 0.9% saline solution for 10 days and 08 rats treated for 10 days with dexamethasone (2mg/kg animal weight). Results: Histological analysis detected a mild cardiac hypertrophy and 15% reduction of collagen located in the aorta of animals treated with glucocorticoid when compared to the control group. Conclusion: We conclude that treatment with dexamethasone for a period of 10 consecutive days is able to promote morphological changes in the structure of the heart chamber and, impair morphological structure of aorta.

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