247 The medicinal and antitumour mushroom, agaricus blazei murill, activates NF-KB via TLR2 in monocytic cells and induces expression of cell surface markers and production of cytokines in human monocyte-derived dendritic cells (MDDC) in vitro

ABSTRACT The gut microbiota may be important in the postnatal development of the immune system and hence may influence the prevalence of atopic diseases. Bifidobacteria are the most numerous bacteria in the guts of infants, and the presence or absence of certain species could be important in determining the geographic incidence of atopic diseases. We compared the fecal populations of bifidobacteria from children aged 25 to 35 days in Ghana (which has a low prevalence of atopy), New Zealand, and the United Kingdom (high-prevalence countries). Natal origin influenced the detection of bifidobacterial species in that fecal samples from Ghana almost all contained Bifidobacterium infantis whereas those of the other children did not. Choosing species on the basis of our bacteriological results, we tested bifidobacterial preparations for their effects on cell surface markers and cytokine production by dendritic cells harvested from cord blood. Species-specific effects on the expression of the dendritic-cell activation marker CD83 and the production of interleukin-10 (IL-10) were observed. Whereas CD83 expression was increased and IL-10 production was induced by Bifidobacterium bifidum, Bifidobacterium longum, and Bifidobacterium pseudocatenulatum, B. infantis failed to produce these effects. We concluded that B. infantis does not trigger the activation of dendritic cells to the degree necessary to initiate an immune response but that B. bifidum, B. longum, and B. pseudocatenulatum induce a Th2-driven immune response. A hypothesis is presented to link our observations to the prevalence of atopic diseases in different countries.

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Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells

Nature Communications ◽

10.1038/s41467-020-15326-5 ◽

2020 ◽

Vol 11 (1) ◽

Cited By ~ 2

Author(s):

Alvaro Plaza Reyes ◽

Sandra Petrus-Reurer ◽

Sara Padrell Sánchez ◽

Pankaj Kumar ◽

Iyadh Douagi ◽

...

Keyword(s):

Cell Surface ◽

Retinal Pigment Epithelial ◽

Retinal Pigment ◽

Surface Markers ◽

Cell Surface Markers ◽

In Vitro Differentiation ◽

Rpe Cells ◽

Pigment Epithelial ◽

Differentiation Efficiency

AbstractIn vitro differentiation of human pluripotent stem cells into functional retinal pigment epithelial (RPE) cells provides a potentially unlimited source for cell based reparative therapy of age-related macular degeneration. Although the inherent pigmentation of the RPE cells have been useful to grossly evaluate differentiation efficiency and allowed manual isolation of pigmented structures, accurate quantification and automated isolation has been challenging. To address this issue, here we perform a comprehensive antibody screening and identify cell surface markers for RPE cells. We show that these markers can be used to isolate RPE cells during in vitro differentiation and to track, quantify and improve differentiation efficiency. Finally, these surface markers aided to develop a robust, direct and scalable monolayer differentiation protocol on human recombinant laminin-111 and −521 without the need for manual isolation.

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In vitro cell surface markers are insufficient to identify in vivo/in situ multipotent synovial mesenchymal stem cells isolated from normal or osteoarthritic knees

Osteoarthritis and Cartilage ◽

10.1016/j.joca.2017.02.057 ◽

2017 ◽

Vol 25 ◽

pp. S27-S28

Author(s):

N. Aljezani ◽

A. Affan ◽

P. Railton ◽

J. Powell ◽

R. Krawetz

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Cell Surface ◽

Surface Markers ◽

Cell Surface Markers

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MODULATION OF CELL SURFACE MARKERS ON NK-LIKE T LYMPHOCYTES BY USING IL-2, IL-7 OR IL-12 IN VITRO STIMULATION

Cytokine ◽

10.1006/cyto.2000.0733 ◽

2000 ◽

Vol 12 (9) ◽

pp. 1385-1390 ◽

Cited By ~ 19

Author(s):

B Zoll ◽

P Lefterova ◽

O Ebert ◽

D Huhn ◽

A von Ruecker ◽

...

Keyword(s):

T Lymphocytes ◽

Cell Surface ◽

Surface Markers ◽

Cell Surface Markers

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The role of cell surface markers and enamel matrix derivatives on human periodontal ligament mesenchymal progenitor responses in vitro

Biomaterials ◽

10.1016/j.biomaterials.2011.06.043 ◽

2011 ◽

Vol 32 (30) ◽

pp. 7375-7388 ◽

Cited By ~ 21

Author(s):

Philippe Kémoun ◽

Stan Gronthos ◽

Malcolm L. Snead ◽

Jacqueline Rue ◽

Bruno Courtois ◽

...

Keyword(s):

Cell Surface ◽

Periodontal Ligament ◽

Surface Markers ◽

Cell Surface Markers ◽

Enamel Matrix ◽

Enamel Matrix Derivatives ◽

Matrix Derivatives

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Phenotype Characterization of Human Adipose-Derived Stem Cell by Flow Cytometry

10.20944/preprints202109.0139.v1 ◽

2021 ◽

Author(s):

Celeste Limoli ◽

Paolo Giuseppe Limoli ◽

Marcella Nebbioso

Keyword(s):

Adipose Tissue ◽

Cell Surface ◽

Stromal Vascular Fraction ◽

Surface Markers ◽

Adipose Derived Stem Cells ◽

Cell Surface Markers ◽

Cell Surface Antigens ◽

Adipose Derived Stem Cell

Background: Developing an efficient and standardized method to isolate and characterize adipose-derived stem cells (ASCs) from the stromal vascular fraction (SVF) of the adipose tissue for clinical application represents one of the major challenges in cell therapy and tissue engineering. Methods: In this study, we proposed an innovative, non-enzymatic protocol to collect clinically useful ASCs within freshly isolated SVF from adipose tissue by centrifugation of the infranatant portion of lipoaspirate and to determine the characteristic cytofluorimetric pattern, prior to in vitro culture. Results: The SVF yielded a mean of 73,32 \pm\ 10,89% cell viability evaluated with CALCEINA-FITC, i.e. cell-permeant dye. The ASCs were positive for PC7-labeled mAb anti-CD34 and negative for both PE-labeled mAb anti-CD31 and APC-labeled mAb anti-CD45. The frequency of ASCs estimated according to the panel of cell surface markers used was 51,06%\ \pm 5,26% versus the unstained ASCs subpopulation that was 0,74%\pm0,84% (P<0.0001). The ASCs events/\muL were 1602,13/\muL \pm 731,87/\muL. Conclusion: Our findings suggested that ASCs found in freshly isolated adipose SVF obtained by centrifugation of lipoaspirate can be immunophenotypically identified with a basic panel of cell surface markers. These findings aimed to provide standardization and contribute to reducing the inconsistency on reported cell surface antigens of ASC derived from the existing literature.

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Expression of v-rel induces mature B-cell lines that reflect the diversity of avian immunoglobulin heavy- and light-chain rearrangements

Molecular and Cellular Biology ◽

10.1128/mcb.8.12.5358-5368.1988 ◽

1988 ◽

Vol 8 (12) ◽

pp. 5358-5368

Author(s):

C F Barth ◽

E H Humphries

Keyword(s):

Cell Surface ◽

B Cell ◽

Cell Lines ◽

Light Chain ◽

Lymphoid Cells ◽

Surface Markers ◽

Cell Surface Markers ◽

Chain Locus ◽

Light Chain Locus

The infection of newly hatched chickens with reticuloendotheliosis virus strain T (REV-T) and a nonimmunosuppressive helper virus, chicken syncytial virus, induces rapidly metastatic B-cell lymphomas. In vivo analysis of these tumors with monoclonal antibodies detected the expression of the B-cell surface markers immunoglobulin M (IgM), CIa, Bu2, and CLA-1, but not IgG, Bu1, or a T-cell surface marker, CT-1. Cell lines derived from tumors exhibited the same pattern of staining, suggesting that expression of cell surface markers does not change during in vitro cell line development. All cell lines examined synthesized IgM in varying amounts. Northern (RNA blot) analysis confirmed abundant expression of v-rel mRNA, and Southern analysis revealed rearrangement of both heavy- and light-chain immunoglobulin loci. Analysis of the light-chain locus demonstrated that 20 of 22 lines contained a single rearranged allele. With respect to specific restriction enzyme sites within the V lambda 1 gene, the active allele in any given clone was either diversified or nondiversified. In contrast, examination of the heavy-chain loci within these lines demonstrated that 16 of the 22 had both alleles rearranged. Further diversification of the V lambda 1 locus did not occur after prolonged in vitro passage of the cell lines. We propose that v-rel expression arrests diversification of the light-chain locus in these lymphoid cells, allowing the production of stable, clonal B-cell populations. The development of these and similar cell lines will make it possible to identify specific stages of avian lymphoid ontogeny and to study the mechanism of rearrangement and diversification in the avian B lymphocyte.

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Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

European Journal of Dentistry ◽

10.1055/s-0040-1719213 ◽

2020 ◽

Author(s):

Alexander Patera Nugraha ◽

Fedik Abdul Rantam ◽

Ida Bagus Narmada ◽

Diah Savitri Ernawati ◽

Igo Syaiful Ihsan

Keyword(s):

Flow Cytometry ◽

Stem Cell ◽

Cell Surface ◽

Osteogenic Differentiation ◽

Oryctolagus Cuniculus ◽

Surface Markers ◽

Cell Surface Markers ◽

Alizarin Red ◽

Regenerative Dentistry

Abstract Objective This study aims to confirm whether the GDMSCs isolated from rabbit’s (Oryctolagus cuniculus) gingiva are mesenchymal stem cells (MSCs). Materials and Methods This study design was partly quasi-experimental with an observational design. GDMSCs were isolated from the gingiva of healthy male rabbits (O. cuniculus) (n = 2), 6 months old, and 3 to 5 kg of body weight. The specific cell surface markers of MSCs; clusters of differentiation (CD), namely, CD44, CD73, CD90, CD105, and CD200 expressions; and hematopoietic stem cell surface markers CD34 and CD45 were examined using flow cytometry and immunohistochemistry with immunofluorescence. The osteogenic differentiation of isolated GDMSCs was examined using alizarin red staining. Results GDMSCs in the fourth passage showed a spindle-like formation and fibroblast-like cells that attached to the base of the culture plate. GDMSCs were MSCs that positively expressed CD44, CD73, CD90, CD105, and CD200 but did not express CD34 and CD45 when examined using flow cytometry and immunohistochemical analysis. GDMSCs had osteogenic differentiation confirmed by calcified deposits in vitro with a red–violet and brownish color after alizarin red staining. Conclusion GDMSCs isolated from the rabbits (O. cuniculus) were confirmed as MSCs in vitro documented using immunohistochemistry and flow cytometry. GDMSCs can differentiate into osteogenic lineage in vitro that may be suitable for regenerative dentistry.

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