ePS6.01 Targeted analysis of volatile organic compounds for detection of Pseudomonas aeruginosa in cystic fibrosis patients by exhaled breath analysis

The aim of the present study was to investigate the ability of breath analysis to distinguish lung cancer (LC) patients from patients with other respiratory diseases and healthy people. The population sample consisted of 51 patients with confirmed LC, 38 patients with pathological computed tomography (CT) findings not diagnosed with LC, and 53 healthy controls. The concentrations of 19 volatile organic compounds (VOCs) were quantified in the exhaled breath of study participants by solid phase microextraction (SPME) of the VOCs and subsequent gas chromatography-mass spectrometry (GC-MS) analysis. Kruskal–Wallis and Mann–Whitney tests were used to identify significant differences between subgroups. Machine learning methods were used to determine the discriminant power of the method. Several compounds were found to differ significantly between LC patients and healthy controls. Strong associations were identified for 2-propanol, 1-propanol, toluene, ethylbenzene, and styrene (p-values < 0.001–0.006). These associations remained significant when ambient air concentrations were subtracted from breath concentrations. VOC levels were found to be affected by ambient air concentrations and a few by smoking status. The random forest machine learning algorithm achieved a correct classification of patients of 88.5% (area under the curve—AUC 0.94). However, none of the methods used achieved adequate discrimination between LC patients and patients with abnormal computed tomography (CT) findings. Biomarker sets, consisting mainly of the exogenous monoaromatic compounds and 1- and 2- propanol, adequately discriminated LC patients from healthy controls. The breath concentrations of these compounds may reflect the alterations in patient’s physiological and biochemical status and perhaps can be used as probes for the investigation of these statuses or normalization of patient-related factors in breath analysis.

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Volatile organic compounds in the exhaled breath of young patients with cystic fibrosis

European Respiratory Journal ◽

10.1183/09031936.06.00085105 ◽

2006 ◽

Vol 27 (5) ◽

pp. 929-936 ◽

Cited By ~ 100

Author(s):

M. Barker ◽

M. Hengst ◽

J. Schmid ◽

H-J. Buers ◽

B. Mittermaier ◽

...

Keyword(s):

Cystic Fibrosis ◽

Volatile Organic Compounds ◽

Organic Compounds ◽

Young Patients ◽

Exhaled Breath ◽

Volatile Organic

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Volatile Organic Compounds in Exhaled Breath of Subjects with Cystic Fibrosis and Healthy Controls.

10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a1198 ◽

2009 ◽

Author(s):

C Robroeks ◽

J Hendriks ◽

Q Jobsis ◽

J Dallinga ◽

J Berkel ◽

...

Keyword(s):

Cystic Fibrosis ◽

Volatile Organic Compounds ◽

Organic Compounds ◽

Exhaled Breath ◽

Healthy Controls ◽

Volatile Organic

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Exhaled Breath Analysis in Tuberculosis Case Detection: The New Horizon

Nepal Journal of Epidemiology ◽

10.3126/nje.v3i2.8509 ◽

2013 ◽

Vol 3 (2) ◽

pp. 243-244

Author(s):

Ranabir Pal ◽

S Dahal ◽

A Gurung

Keyword(s):

Volatile Organic Compounds ◽

Organic Compounds ◽

Gaseous Mixture ◽

Nobel Prize Winner ◽

Exhaled Breath ◽

Tuberculosis Case ◽

Exhaled Breath Analysis ◽

Organ Systems ◽

Volatile Organic ◽

Health And Disease

Sixty years ago, Nobel Prize winner Linus Pauling proposed the concept that human breath is a complex and dynamic gaseous mixture of more than 200 different endogenous volatile organic compounds (VOCs) that are continually being released in different quantities within the internal environment during health and disease. The researchers estimate that more than a thousand chemicals produced in different organ-systems as metabolic end products come out every minute through the respiratory tract. Volatile organic compounds and other products of oxidative stress are a big chunk among these exhaled breath elements.DOI: http://doi.dx.org/10.3126/nje.v3i2.8509

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Targeted exhaled breath analysis for detection of Pseudomonas aeruginosa in cystic fibrosis patients

10.1183/13993003.congress-2020.685 ◽

2020 ◽

Author(s):

Renate Kos ◽

Paul Brinkman ◽

Anne H Neerincx ◽

Tamara Paff ◽

Marije G Gerritsen ◽

...

Keyword(s):

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

Breath Analysis ◽

Exhaled Breath ◽

Exhaled Breath Analysis

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Breath Tests in Respiratory and Critical Care Medicine: From Research to Practice in Current Perspectives

BioMed Research International ◽

10.1155/2013/702896 ◽

2013 ◽

Vol 2013 ◽

pp. 1-20 ◽

Cited By ~ 4

Author(s):

Attapon Cheepsattayakorn ◽

Ruangrong Cheepsattayakorn

Keyword(s):

Volatile Organic Compounds ◽

Organic Compounds ◽

Exhaled Breath ◽

Home Setting ◽

Exhaled Breath Analysis ◽

Low Concentrations ◽

Volatile Organic ◽

Normal Human ◽

Novel Method ◽

The Individual

Today, exhaled nitric oxide has been studied the most, and most researches have now focusd on asthma. More than a thousand different volatile organic compounds have been observed in low concentrations in normal human breath. Alkanes and methylalkanes, the majority of breath volatile organic compounds, have been increasingly used by physicians as a novel method to diagnose many diseases without discomforts of invasive procedures. None of the individual exhaled volatile organic compound alone is specific for disease. Exhaled breath analysis techniques may be available to diagnose and monitor the diseases in home setting when their sensitivity and specificity are improved in the future.

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Macromolecular hexa-asymmetric zinc(II) phthalocyanines bearing triazole-modified triphenylene core: Synthesis, spectroscopy and analysis towards volatile organic compounds on Surface Acoustic Wave devices

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424619500342 ◽

2019 ◽

Vol 23 (04n05) ◽

pp. 477-488 ◽

Cited By ~ 5

Author(s):

Funda Kus ◽

Cihat Tasaltin ◽

Mohamad Albakour ◽

Ayşe Gül Gürek ◽

İlke Gürol

Keyword(s):

Volatile Organic Compounds ◽

Acoustic Wave ◽

Surface Acoustic Wave ◽

Organic Compounds ◽

Spectroscopic Techniques ◽

Exhaled Breath ◽

H Nmr ◽

Exhaled Breath Analysis ◽

Volatile Organic ◽

Acoustic Wave Devices

The synthesis and characterization of novel asymmetric zinc(II) phthalocyanines (4–9) and their linking through peripheral and nonperipheral positions on the phthalocyanine ring via click coupling to alkyne-functionalized 2,3,6,7,10,11-hexakis(prop-2-ynyloxy)triphenylene core are described for the first time. These phthalocyanines (Pcs) (4–12) were characterized by elemental analysis and different spectroscopic techniques such as UV-vis, 1H-NMR, FT-IR and mass spectroscopy. Furthermore, the utilization of thin films of novel Pcs as a sensitive layer for detection of lung cancer from exhaled human breath at room temperature under exposure to marker volatile organic compounds (VOCs) are presented. The developed sensors were tested for acetone, ethanol, [Formula: see text]-hexane, toluene, chloroform and isoprene in a range of 300–14560 ppm. The obtained results have confirmed the possibility of utilization of Pc-based Surface Acoustic Wave (SAW) sensors for medical diagnosis based on exhaled breath analysis.

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