Is cold atmospheric plasma an option for bladder cancer treatment? In vitro studies

Background: Over the past five years, the cold atmospheric plasma-activated solutions (PAS) have shown their promissing application in cancer treatment. Similar as the common direct cold plasma treatment, PAS shows a selective anti-cancer capacity in vitro and in vivo. However, different from the direct cold atmospheric plasma (CAP) treatment, PAS can be stored for a long time and can be used without dependence on a CAP device. The research on PAS is gradually becoming a hot topic in plasma medicine. Objectives: In this review, we gave a concise but comprehensive summary on key topics about PAS including the development, current status, as well as the main conclusions about the anti-cancer mechanism achieved in past years. The approaches to make strong and stable PAS are also summarized.

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INNV-08. COMPARATIVE STUDY OF THE CANCER TREATMENT POTENTIAL EFFECT OF TUMOR-TREATING FIELDS AND COLD ATMOSPHERIC PLASMA

Neuro-Oncology ◽

10.1093/neuonc/noaa215.492 ◽

2020 ◽

Vol 22 (Supplement_2) ◽

pp. ii118-ii118

Author(s):

Xiaoliang Yao ◽

Isaac Goldstein ◽

Li Lin ◽

Jonathan Sherman ◽

Michael Keidar

Keyword(s):

Cell Viability ◽

Cancer Treatment ◽

Brain Cancer ◽

Room Temperature ◽

Treatment Time ◽

In Vitro Study ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma ◽

Tumor Treating Fields

Abstract INTRODUCTION Cold Atmospheric Plasma (CAP) is ionized gas close to room temperature that generates reactive oxygen and nitrogen species, an electromagnetic field (EMF) and UV, which are selectively cytotoxic to cancer cells. Tumor Treating Fields (TTFields) is an FDA approved cancer treatment that utilizes “low intensity” and “intermediate frequency” alternating electric fields to produce an inhibitory effect on cancerous cells. OBJECTIVES In this study, we directly compared CAP and TTFields treatments using U87 glioblastoma tumor cells. We aimed to assess the relative merits of both technologies as potential approaches for brain cancer treatment. METHODS In this in vitro study, the cells were treated with either CAP or TTFields at room temperature. In both cases in order the optimize the treatment parameters, the energy we applied was varied for both methods by changing the power and time of the treatment. The resulting cell viability changes were measured 72 hours after treatment. RESULTS The results revealed that increased energy resulted in decreased cell viability of U87 glioblastoma tumor cells with both CAP and TTFields treatment. The rate of U87 glioblastoma tumor cell viability under CAP treatment was 80% of the original viability after using 4.8J of energy, compared with 15,752J of energy with TTFields treatment. Additionally, the CAP displayed significantly greater time-efficiency as compared to TTFields. For instance, the most effective TTFields treatment took 4 hours, while the CAP treatment displayed a comparable effect on tumor cell viability with a treatment time of 30 seconds. CONCLUSIONS This preliminary in vitro study indicates that CAP treatment is potentially a more favorable effective method of brain cancer therapy. This data indicates that CAP is significantly more energy-efficient and requires significantly less treatment time than TTFields. Future research looks to compare the 2 treatments in an intracranial mouse xenograft model.

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The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens—A Systematic Review of In-Vitro Studies

Antibiotics ◽

10.3390/antibiotics10020211 ◽

2021 ◽

Vol 10 (2) ◽

pp. 211

Author(s):

Gert Jungbauer ◽

Dominick Moser ◽

Steffen Müller ◽

Wolfgang Pfister ◽

Anton Sculean ◽

...

Keyword(s):

Randomized Clinical Trials ◽

Assessment Tool ◽

Treatment Time ◽

Antimicrobial Effect ◽

In Vitro Studies ◽

Atmospheric Plasma ◽

Promising Alternative ◽

Cold Atmospheric Plasma

Interest in the application of cold atmospheric plasma (CAP) in the medical field has been increasing. Indications in dentistry are surface modifications and antimicrobial interventions. The antimicrobial effect of CAP is mainly attributed to the generation of reactive oxygen and reactive nitrogen species. The aim of this article is to systematically review the available evidence from in-vitro studies on the antimicrobial effect of CAP on dental pathogens. A database search was performed (PubMed, Embase, Scopus). Data concerning the device parameters, experimental set-ups and microbial cultivation were extracted. The quality of the studies was evaluated using a newly designed assessment tool. 55 studies were included (quality score 31–92%). The reduction factors varied strongly among the publications although clusters could be identified between groups of set pathogen, working gases, and treatment time intervals. A time-dependent increase of the antimicrobial effect was observed throughout the studies. CAP may be a promising alternative for antimicrobial treatment in a clinically feasible application time. The introduced standardized protocol is able to compare the outcome and quality of in-vitro studies. Further studies, including multi-species biofilm models, are needed to specify the application parameters of CAP before CAP should be tested in randomized clinical trials.

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Network pharmacology-based analysis for unraveling potential cancer-related molecular targets of Egyptian propolis phytoconstituents accompanied with molecular docking and in vitro studies

RSC Advances ◽

10.1039/d1ra01390d ◽

2021 ◽

Vol 11 (19) ◽

pp. 11610-11626

Author(s):

Reham S. Ibrahim ◽

Alaa A. El-Banna

Keyword(s):

Molecular Docking ◽

Cancer Treatment ◽

Mechanism Of Action ◽

Molecular Targets ◽

Integrated Approach ◽

In Vitro Studies ◽

Network Pharmacology ◽

Multi Level ◽

Potential Cancer

Multi-level mechanism of action of propolis constituents in cancer treatment using an integrated approach of network pharmacology-based analysis, molecular docking and in vitro cytotoxicity testing.

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The synergistic effect of Canady Helios cold atmospheric plasma and a FOLFIRINOX regimen for the treatment of cholangiocarcinoma in vitro

Scientific Reports ◽

10.1038/s41598-021-88451-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Olivia Jones ◽

Xiaoqian Cheng ◽

Saravana R. K. Murthy ◽

Lawan Ly ◽

Taisen Zhuang ◽

...

Keyword(s):

Cell Viability ◽

Chemotherapy Regimen ◽

Synergetic Effect ◽

Atmospheric Plasma ◽

High Recurrence Rate ◽

Cold Atmospheric Plasma ◽

Tract Cancer ◽

Anticancer Properties ◽

Potential Interactions

AbstractCholangiocarcinoma (CCA) is a rare biliary tract cancer with a low five-year survival rate and high recurrence rate after surgical resection. Currently treatment approaches include systemic chemotherapeutics such as FOLFIRINOX, a chemotherapy regimen is a possible treatment for severe CCA cases. A limitation of this chemotherapy regimen is its toxicity to patients and adverse events. There exists a need for therapies to alleviate the toxicity of a FOLFIRINOX regimen while enhancing or not altering its anticancer properties. Cold atmospheric plasma (CAP) is a technology with a promising future as a selective cancer treatment. It is critical to know the potential interactions between CAP and adjuvant chemotherapeutics. In this study the aim is to characterize the efficacy of FOLFIRINOX and CAP in combination to understand potential synergetic effect on CCA cells. FOLFIRINOX treatment alone at the highest dose tested (53.8 µM fluorouracil, 13.7 µM Leucovorin, 5.1 µM Irinotecan, and 3.7 µM Oxaliplatin) reduced CCA cell viability to below 20% while CAP treatment alone for 7 min reduced viability to 3% (p < 0.05). An analysis of cell viability, proliferation, and cell cycle demonstrated that CAP in combination with FOLFIRINOX is more effective than either treatment alone at a lower FOLFIRINOX dose of 6.7 µM fluorouracil, 1.7 µM leucovorin, 0.6 µM irinotecan, and 0.5 µM oxaliplatin and a shorter CAP treatment of 1, 3, or 5 min. In conclusion, CAP has the potential to reduce the toxicity burden of FOLFIRINOX and warrants further investigation as an adjuvant therapy.

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Intravesical therapy with paclitaxel-loaded lipid nanoparticle on experimental bladder cancer: In vivo and in vitro studies

European Urology Supplements ◽

10.1016/s1569-9056(03)80259-2 ◽

2003 ◽

Vol 2 (1) ◽

pp. 66

Author(s):

S. Kim ◽

S. Lee ◽

Y. Cho ◽

M. Yoon

Keyword(s):

Bladder Cancer ◽

In Vitro Studies ◽

Intravesical Therapy ◽

Lipid Nanoparticle

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Cold atmospheric plasma coupled with air abrasion in liquid medium for the treatment of peri-implantitis model grown with a complex human biofilm: an in vitro study

Clinical Oral Investigations ◽

10.1007/s00784-021-03949-x ◽

2021 ◽

Author(s):

Wang Lai Hui ◽

Vittoria Perrotti ◽

Adriano Piattelli ◽

Kostya (Ken) Ostrikov ◽

Zhi Fang ◽

...

Keyword(s):

Liquid Medium ◽

Statistical Significance ◽

Atmospheric Plasma ◽

Air Abrasion ◽

Implant Surface ◽

Cold Atmospheric Plasma ◽

Surface Features ◽

Treatment Groups ◽

Surface Decontamination

Abstract Objective Treatment of implants with peri-implantitis is often unsuccessful due to residual microbial biofilm hindering re-osseointegration. The aim of this study was to treat biofilm-grown titanium (Ti) implants with different modalities involving air abrasion (AA) and cold atmospheric plasma (CAP) to compare the effectiveness in surface decontamination and the alteration/preservation of surface topography. Materials and methods Saliva collected from a peri-implantitis patient was used to in vitro develop human biofilm over 35 implants with moderately rough surface. The implants were then mounted onto standardized acrylic blocks simulating peri-implantitis defects and treated with AA (erythritol powder), CAP in a liquid medium, or a combination (COM) of both modalities. The remaining biofilm was measured by crystal violet (CV). Surface features and roughness before and after treatment were assessed by scanning electron microscope (SEM). The data were statistically analyzed using Kruskal-Wallis followed by Tukey’s multiple comparison test. Results In the present peri-implantitis model, the human complex biofilm growth was successful as indicated by the statistical significance between the negative and positive controls. All the treatment groups resulted in a remarkable implant surface decontamination, with values very close to the negative control for AA and COM. Indeed, statistically significant differences in the comparison between the positive control vs. all the treatment groups were found. SEM analysis showed no post-treatment alterations on the implant surface in all the groups. Conclusions Decontamination with AA delivering erythritol with or without CAP in liquid medium demonstrated compelling efficacy in the removal of biofilm from implants. All the tested treatments did not cause qualitative alterations to the Ti surface features. No specific effects of the CAP were observed, although further studies are necessary to assess its potential as monotherapy with different settings or in combination with other decontamination procedures. Clinical relevance CAP is a promising option in the treatment of peri-implantitis because it has potential to improve the elimination of bacterial plaque from implant surfaces, in inaccessible pockets or during open-flap debridement, and should stimulate the process of the re-osseointegration of affected dental implants by not altering surface features and roughness.

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