The Cell Activation Phenomena in the Cold Atmospheric Plasma Cancer Treatment

Background: Over the past five years, the cold atmospheric plasma-activated solutions (PAS) have shown their promissing application in cancer treatment. Similar as the common direct cold plasma treatment, PAS shows a selective anti-cancer capacity in vitro and in vivo. However, different from the direct cold atmospheric plasma (CAP) treatment, PAS can be stored for a long time and can be used without dependence on a CAP device. The research on PAS is gradually becoming a hot topic in plasma medicine. Objectives: In this review, we gave a concise but comprehensive summary on key topics about PAS including the development, current status, as well as the main conclusions about the anti-cancer mechanism achieved in past years. The approaches to make strong and stable PAS are also summarized.

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Micro-Sized Cold Atmospheric Plasma Source for Brain and Breast Cancer Treatment

Plasma Medicine ◽

10.1615/plasmamed.2018026588 ◽

2018 ◽

Vol 8 (2) ◽

pp. 203-215 ◽

Cited By ~ 7

Author(s):

Zhitong Chen ◽

Li Lin ◽

Qinmin Zheng ◽

Jonathan H. Sherman ◽

Jerome Canady ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Treatment ◽

Plasma Source ◽

Breast Cancer Treatment ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma

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Principles of using Cold Atmospheric Plasma Stimulated Media for Cancer Treatment

Scientific Reports ◽

10.1038/srep18339 ◽

2015 ◽

Vol 5 (1) ◽

Cited By ~ 119

Author(s):

Dayun Yan ◽

Annie Talbot ◽

Niki Nourmohammadi ◽

Xiaoqian Cheng ◽

Jerome Canady ◽

...

Keyword(s):

Cancer Treatment ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma

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Cold Atmospheric Plasma Cancer Treatment, a Critical Review

Applied Sciences ◽

10.3390/app11167757 ◽

2021 ◽

Vol 11 (16) ◽

pp. 7757

Author(s):

Dayun Yan ◽

Alisa Malyavko ◽

Qihui Wang ◽

Li Lin ◽

Jonathan H. Sherman ◽

...

Keyword(s):

Cancer Treatment ◽

Molecular Mechanisms ◽

Critical Evaluation ◽

Atmospheric Plasma ◽

Physical Factors ◽

Atmospheric Conditions ◽

Cold Atmospheric Plasma ◽

Anti Cancer ◽

Biological Impacts ◽

Indirect Treatment

Cold atmospheric plasma (CAP) is an ionized gas, the product of a non-equilibrium discharge at atmospheric conditions. Both chemical and physical factors in CAP have been demonstrated to have unique biological impacts in cancer treatment. From a chemical-based perspective, the anti-cancer efficacy is determined by the cellular sensitivity to reactive species. CAP may also be used as a powerful anti-cancer modality based on its physical factors, mainly EM emission. Here, we delve into three CAP cancer treatment approaches, chemically based direct/indirect treatment and physical-based treatment by discussing their basic principles, features, advantages, and drawbacks. This review does not focus on the molecular mechanisms, which have been widely introduced in previous reviews. Based on these approaches and novel adaptive plasma concepts, we discuss the potential clinical application of CAP cancer treatment using a critical evaluation and forward-looking perspectives.

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Portable air-fed cold atmospheric plasma device for postsurgical cancer treatment

Science Advances ◽

10.1126/sciadv.abg5686 ◽

2021 ◽

Vol 7 (36) ◽

Author(s):

Guojun Chen ◽

Zhitong Chen ◽

Zejun Wang ◽

Richard Obenchain ◽

Di Wen ◽

...

Keyword(s):

Cancer Treatment ◽

Atmospheric Plasma ◽

Cold Atmospheric Plasma ◽

Plasma Device

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Mathematical modeling and control for cancer treatment with cold atmospheric plasma jet

Journal of Physics D Applied Physics ◽

10.1088/1361-6463/ab061d ◽

2019 ◽

Vol 52 (18) ◽

pp. 185202 ◽

Cited By ~ 4

Author(s):

Yuanwei Lyu ◽

Li Lin ◽

Eda Gjika ◽

Taeyoung Lee ◽

Michael Keidar

Keyword(s):

Mathematical Modeling ◽

Cancer Treatment ◽

Plasma Jet ◽

Atmospheric Plasma ◽

Modeling And Control ◽

Cold Atmospheric Plasma ◽

And Control

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Cold Atmospheric Plasma Activated Solution: A New Approach for Cancer Treatment

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/187152061806181112124717 ◽

2018 ◽

Vol 18 (6) ◽

pp. 768-768 ◽

Cited By ~ 4

Author(s):

Pankaj Attri

Keyword(s):

Cancer Treatment ◽

Atmospheric Plasma ◽

New Approach ◽

Cold Atmospheric Plasma

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EXTH-10. THE ACTIVATION AND SENSITIZATION OF GLIOBLASTOMA CELLS VIA COLD ATMOSPHERIC PLASMA TREATMENT

Neuro-Oncology ◽

10.1093/neuonc/noz175.344 ◽

2019 ◽

Vol 21 (Supplement_6) ◽

pp. vi84-vi84

Author(s):

Jonathan Sherman ◽

Dayun Yan ◽

Eda Gjika ◽

Michael Keidar

Keyword(s):

Cell Viability ◽

Plasma Treatment ◽

Cell Activation ◽

Radiation Treatment ◽

In Vitro Study ◽

Atmospheric Plasma ◽

Discharge Process ◽

H2o2 Treatment ◽

Cold Atmospheric Plasma

Abstract BACKGROUND Treatment of glioblastoma multiforme (GBM) continues to remain a challenge using conventional treatment. Through an in vitro study we assessed the efficacy of our novel cold atmospheric plasma technology (CAP) to sensitize GBM cells to temozolomide (TMZ). METHODS The CAP jet is formed through the discharge (Pk-Pk: 5.8 kV) between a ring grounded cathode and a central anode and with He flow through a glass tube. The discharge process is driven by an AC high voltage (3.16 kV) with a frequency of 12.5 kHz. Human glioblastoma (U87MG) cells were cultured in DMEM supplemented by 1% (v/v) penicillin and streptomycin solution and 10% (v/v) FBS. CAP was delivered to U87 cells in a 96-well plate for 1 min in combination with 10 and 15 μM H2O2. The cell viability was measured by using the MTT assay. We then tested TMZ concentrations of 10 and 50 uM. Cell viability was monitored with the Cell Titer Glo 2.0. luminescent assay. All experiments were performed in triplicate and were independently repeated at least 3 times. RESULTS We identified an activation state of U87MG cells after the plasma treatment. This activation state resulted in GBM cells sensitized to reactive species identified by decreased cell viability after treatment with H2O2 as compared to the H2O2 treatment alone (p< 0.005). In addition, the plasma-activated cells were sensitized to TMZ. Cells treated with CAP in combination with TMZ displayed decreased cell viability at TMZ concentrations of (10 uM) (p< 0.05) and (50 uM) (p< 0.005) as compared to TMZ alone. CONCLUSIONS This study demonstrates the activation phenomenon on GBM cells via direct CAP treatment. Due to this activation, the GBM cells were sensitized to both H2O2 and TMZ identified via decreased cell viability. Future work looks to assess this effect of cell activation/sensitization with chemotherapy plus radiation treatment.

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