scholarly journals Molecular evidence for blocking erucic acid synthesis in rapeseed (Brassica napus L.) by a two-base-pair deletion in FAE1 (fatty acid elongase 1)

2015 ◽  
Vol 14 (7) ◽  
pp. 1251-1260 ◽  
Author(s):  
Lei WU ◽  
Yan-li JIA ◽  
Gang WU ◽  
Chang-ming LU
Keyword(s):  
Fatty Acid ◽  
Brassica Napus ◽  
Erucic Acid ◽  
Base Pair ◽  
Acid Synthesis ◽  
Molecular Evidence ◽  
Brassica Napus L ◽  
Fatty Acid Elongase ◽  
Base Pair Deletion

A STUDY OF ERUCIC ACID ALLELES IN DIGENOMIC RAPESEED (Brassica napus L.)

1981 ◽  
Vol 61 (2) ◽  
pp. 198-202 ◽  
Author(s):  
I. J. ANAND ◽  
R. K. DOWNEY
Keyword(s):  
Fatty Acid ◽  
Brassica Napus ◽  
Erucic Acid ◽  
Direct Evidence ◽  
Brassica Campestris ◽  
Fatty Acid Analysis ◽  
Interspecific Crosses ◽  
Seed Oils ◽  
Brassica Napus L ◽  
Brassica Crops

Five genes have been identified in Brassica crops which control the level of synthesis of the fatty acid, erucic, in their seed oils. These genes, designated e, Ea, Eb, Ec, and Ed, act in an additive manner and result in erucic acid levels of < 1, 10, 15, 30 and 3.5, respectively. No direct evidence has yet been obtained to show that these genes are true alleles. Selected plants of the amphidiploid species Brassica napus L. with erucic acid contents of 7–8% and a genotype of EdEdee were reciprocally crossed with selected plants with erucic acid levels of [Formula: see text] and a genotype of Eaeee. Fatty acid analysis of F1 and backcross seed demonstrated that the genes Ed and Ea in the parents used were in the same genome and were truly allelic. Interspecific crosses were made between these B. napus parents and selected zero erucic acid plants of Brassica campestris L. (genotype "ee") to determine whether the genes Ed and Ea resided in the oleracea or the campestris genome of B. napus parents. Fatty acid analysis of F1 and backcross seed from these interspecific crosses suggest that the alleles of Ed and Ea are located on chromosomes of the oleracea genome.

The role of plastidial transporters in developing embryos of oilseed rape (Brassica napus L.) for fatty acid synthesis

2000 ◽  
Vol 28 (6) ◽  
pp. 665-666 ◽  
Author(s):  
S. E. Kubis ◽  
S. Rawsthorne
Keyword(s):  
Fatty Acid ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Fatty Acid Synthesis ◽  
Developmental Stages ◽  
Acid Synthesis ◽  
Brassica Napus L

The phosphoenolpyruvate transporter (PPT) is one of several important transporters for channelling carbon intermediates utilized for fatty acid synthesis and other plastidial pathways from the cytosol into the plastid. In this paper we show results on how the activity of the PPT changes between two important, physiologically different developmental stages of oilseed rape embryos.

scholarly journals Inhibition of the glucose-6-phosphate transporter in oilseed rape (Brassica napus L.) plastids by acyl-CoA thioesters reduces fatty acid synthesis

2000 ◽  
Vol 352 (2) ◽  
pp. 525-532 ◽  
Author(s):  
Simon R. FOX ◽  
Lionel M. HILL ◽  
Stephen RAWSTHORNE ◽  
Matthew J. HILLS
Keyword(s):  
Fatty Acid ◽  
Brassica Napus ◽  
Oilseed Rape ◽  
Acid Synthesis ◽  
Brassica Napus L ◽  
Coa Ligase ◽  
Chain Acyl ◽  
Coa Thioesters ◽  
Long Chain

Addition of oleoyl-CoA (1µM), or other acyl-CoA thioesters with a chain length of C16 or greater, to oilseed rape plastids (Brassica napus L.) inhibited the rate of D-glucose 6-phosphate (Glc6P) uptake by 70% after 2min. The IC50 value for oleoyl-CoA inhibition of the transporter was approx. 0.2–0.3µM. Inhibition was alleviated by the addition of acyl-CoA binding protein (ACBP) or BSA at slightly higher concentrations. Oleic acid (5–25µM), Tween 40 (10µM), Triton-X 100 (10µM) and palmitoylcarnitine (5µM) had no effect on Glc6P uptake. The uptake of [1-14C]Glc6P occurred in exchange for Pi, 3-phosphoglycerate or Glc6P at a typical rate of 30nmol Glc6P/min per unit of glyceraldehyde-3-phosphate dehydrogenase (NADP+). The Km(app) of the Glc6P transporter for Glc6P was 100µM. Neither CoA (0.3mM) nor ATP (3mM) inhibited Glc6P uptake, but the transporter was inhibited by 72% when ATP and CoA were added together. This inhibition was attributable to the synthesis of acyl-CoA thioesters, predominantly oleoyl-CoA and palmitoyl-CoA, by long-chain fatty acid-CoA ligase (EC 6.2.1.3) from endogenous fatty acids in the plastid preparations. Acyl-CoA thioesters did not inhibit the uptake of [2-14C]pyruvate or D-[1-14C]glucose into plastids. In vivo quantities of oleoyl-CoA and other long-chain acyl-CoA thioesters were lower than those for ACBP in early cotyledonary embryos, 0.7±0.2pmol/embryo and 2.2±0.2pmol/embryo respectively, but in late cotyledonary embryos quantities of long-chain acyl-CoA thioesters were greater than ACBP, 3±0.4pmol/embryo and 1.9±0.2pmol/embryo respectively.

INHERITANCE OF FATTY ACID COMPOSITION IN WINTER FORMS OF RAPESEED, BRASSICA NAPUS

1969 ◽  
Vol 49 (3) ◽  
pp. 313-319 ◽  
Author(s):  
J. Krzymanski ◽  
R. K. Downey
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Erucic Acid ◽  
Correlation Coefficients ◽  
Eicosenoic Acid ◽  
Fatty Acid Analysis ◽  
Long Chain Fatty Acids ◽  
Brassica Napus L ◽  
Winter Rapeseed

The transfer of the zero erucic acid characteristic from spring to winter rapeseed (Brassica napus L.) and the identification of a new allele determining the erucic acid level in rapeseed oil are reported. Fatty acid analysis of F2 seed from the cross zero × low (7%) erucic acid winter rapeseed parents supported the hypothesis that, in these strains, one gene pair governs the level of erucic acid, and that each, allele contributes approximately 3.5% erucic and 6% eicosenoic acid to the seed oil. Gene action is similar to other alleles in this series, in that the genes display no dominance and act in an additive manner. The long-chain fatty acids, erucic and eicosenoic, were each significantly negatively correlated with the 18 carbon fatty acids, oleic and linoleic. Within each of the three F2 genotypes, correlation coefficients between oleic and linoleic were also negative and significant.

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