Preparation and efficacy evaluation of Paenibacillus polymyxa KM2501-1 microbial organic fertilizer against root-knot nematodes

Abstract Background Microbial cultures are extensively used as environment friendly ways for biological control of parasitic pests, including the root-knot nematodes, alternative to the use of chemical nematicides. The present study was conducted to isolate some lytic rhizobacteria and examine their nematicidal activity against Meloidogyne incognita J2 mortality and egg hatching in in vitro test. Results Lytic Rhizobacteria were isolated from soil samples adhering to tomato plant roots from different localities at Giza governorate, Egypt. Six bacterial isolates, exhibited high efficacy against root-knot nematodes, were identified based on the analysis of the 16S rRNA gene sequence as Pseudomonas aeruginosa, Paenibacillus polymyxa, Lysinibacillus sphaericus, Bacillus cereus, Bacillus subtilis, and Achromobacter xylosoxidans. These strains showed high production of chitinase, chitosanase, and protease using colloidal chitin and soluble chitosan as carbon sources. Two strains, Paenibacillus polymyxa and Bacillus subtilis, produced the highest levels of chitinase in the media. Except for Pseudomonas aeruginosa, all the bacterial strains produced high levels of chitosanase. Conclusion The results revealed that high amounts of protease were, however, secreted by Pseudomonas aeruginosa as compared to the other strains. In in vitro tests, all the bacterial culture filtrates potentially displayed nematicidal effect in M. incognita egg hatching and an obvious increase in J2 mortality as compared to control. Paenibacillus polymyxa caused 100% juvenile mortality followed by Bacillus subtilis 97.25%, Bacillus cereus 94%, Achromobacter xylosoxidans 93%, Lysinibacillus sphaericus 92%, and Pseudomonas aeruginosa 84.29% after 48 h of exposure, as compared to control.

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Novel-integrated process for production of bio-organic fertilizer via swine manure composting

Environmental Engineering Research ◽

10.4491/eer.2019.522 ◽

2020 ◽

Vol 26 (2) ◽

pp. 190522-0

Author(s):

Yan Chen ◽

Xiaoyu Li ◽

Shuying Li ◽

Yongping Xu

Keyword(s):

Disease Incidence ◽

Organic Fertilizer ◽

Swine Manure ◽

Paenibacillus Polymyxa ◽

Single Step ◽

Organic Fertilizers ◽

Control Group ◽

Promoting Effect ◽

Significant Difference ◽

The Difference

The traditional method of producing bio-organic fertilizer contains two consecutive composting stages which, however, is time-consuming and requires high input of energy and cost. This study attempted to combine two consecutive stages into one single step. Paenibacillus polymyxa, as an antagonistic strain to control anthracnose in Chinese Pakchoi, was used as inoculum to obtain bio-organic fertilizers through two approaches: two consecutive stages (TCS) composting as control and the proposed one step through (OST) composting. The resulting two bio-organic fertilizers produced by TCS and OST were tested for the disease incidence and disease resistant index, and the fertility on growth of Chinese Pakchoi. The results of relative abundance of P. polymyxa showed no significant difference between two kinds of bio-organic fertilizers in phylum, family and genus. The disease incidence of bio-organic fertilizers produced by OST and TCS reduced by 61.40% and 68.42%, respectively, as compared to the control group, where the difference was not significant. The growth promoting effect was remarkable, although the difference in the fertility on the growth was not significant. Compared with the traditional TCS method, the novel OST method could improve efficiency of bio-organic fertilizer production by 42.86% per year according to this study.

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ESTIMATION OF CONSUMPTIVE USE OF POTATO CROP PIANTED UNDER DRIP IRRIGATION SYSTEM BY USING SWRT TECHNOLOGY

Al-Qadisiyah Journal For Agriculture Sciences (QJAS) (P-ISSN 2077-5822 E-ISSN 2617-1479) ◽

10.33794/qjas.vol10.iss1.109 ◽

2020 ◽

Vol 10 (1) ◽

pp. 271-282

Author(s):

J.N. Abedalrahman ◽

R.J. Mansor ◽

D.R. Abass

Keyword(s):

Water Consumption ◽

Drip Irrigation ◽

Soil Depth ◽

Irrigation System ◽

Potato Crop ◽

Block Design ◽

Organic Fertilizer ◽

Control Treatment ◽

Drip Irrigation System ◽

Spring Season

A field experiment was carried out in the field of the College of Agriculture / University of Wasit, located on longitude 45o 50o 33.5o East and latitude 32o 29o 49.8o North, in Spring season of the agricultural season 2019, in order to estimate the water consumption of potato crop using SWRT technology and under the drip irrigation system. The experiment was designed according to Randomized Complete Block Design (RCBD) with three replications and four treatments that include of the SWRT treatment (the use of plastic films under the plant root area in an engineering style), and the treatment of vegetal fertilizer (using Petmos), organic fertilizer (sheep manure), and the control treatment . Potato tubers (Solanum tuberosum L.) var. Burin was planted for spring season on 10/2/2019 at the soil depth of 5-10 cm. The highest reference water consumption for the potato crop during the season was calculated by Najeeb Kharufa, which was 663.03 mm. The highest actual water consumption for the potato crop during the season for the control treatment was 410.1 mm. The results showed increase in the values of the crop coefficient (Kc) in the stages of tubers formation and tubers filling stage as compared to the vegetative and ripening stages, ranged from 1.37-1.92 for the two stages of tubers formation and tubers filling. The SWRT treatment gave the highest water use efficiency during the season, was 3.46 kg m-3 .

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Validation and Efficacy Evaluation of an Electronic Weight-Estimate Device Made By Korean Children's Data Compared with Broselow Tape

PEDIATRICS ◽

10.1542/peds.137.supplement_3.200a ◽

2016 ◽

Vol 137 (Supplement 3) ◽

pp. 200A-200A

Author(s):

Joongwan Park ◽

Young Ho Kwak ◽

Do Kyun Kim ◽

Ji Hea Jung ◽

Jae Yun Jung

Keyword(s):

Efficacy Evaluation ◽

Weight Estimate

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Dairy Farmers Empowerment in Subak Anggabaya Penatih Timur Denpasar Bali Indonesia

International Journal of Contemporary Research and Review ◽

10.15520/ijcrr/2018/9/05/510 ◽

2018 ◽

Vol 9 (05) ◽

pp. 20218-20223

Author(s):

I Ketut Arnawa ◽

I Wayan Runa ◽

I Made Sukerta ◽

Putu Sri Astuti ◽

Dan Luh Kadek Budi Martini

Keyword(s):

Rice Straw ◽

Azadirachta Indica ◽

Nutritional Value ◽

Organic Fertilizer ◽

Process Technology ◽

Dairy Farmers ◽

Training Activity ◽

Good Nutrition ◽

And Training ◽

Spraying Method

The main purpose for the application of science and technology for livestock group of cow, is to form a group of skilled and professional livestock, as vaccinators, can take advantage of Azadirachta indica leaves a plant insecticide, apply a good cage sanitation, can produce organic fertilizer from feces of cow, can treat hay as feed to good nutritional value. The method used is education and training. Activity results obtained, that 10 percent of members of the group have managed to become vaccinators cadres, able to implement good sanitation stables, feces of cow (Stomoxys calsitrans) can process technology with EM4 into organic fertilizer and can process the rice straw with urea spraying method, to produce feed hay to the value of good nutrition for growing cattle.

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Exemplar Abstract for Paenibacillus polymyxa (Prażmowski 1880) Ash et al. 1994 emend. Kwak et al. 2020.

The NamesforLife Abstracts ◽

10.1601/ex.16098 ◽

2003 ◽

Author(s):

Charles Thomas Parker ◽

Dorothea Taylor ◽

George M Garrity

Keyword(s):

Paenibacillus Polymyxa

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Field, laboratory, and screenhouse efficacy evaluation of Bt corn for resistance to the Asian corn borer,Ostrinia furnacalis(Guenee), in the Philippines

10.1603/ice.2016.91754 ◽

2016 ◽

Author(s):

Merdelyn Caasi-Lit

Keyword(s):

The Philippines ◽

Bt Corn ◽

Ostrinia Furnacalis ◽

Efficacy Evaluation ◽

Corn Borer ◽

Field Laboratory ◽

Asian Corn Borer

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Efficacy evaluation of the CEUS-LI-RADS-v2017 system in differential diagnosis of liver tumors

Medical Visualization ◽

10.24835/1607-0763-2019-4-57-67 ◽

2019 ◽

pp. 57-67

Author(s):

A. N. Katrich ◽

V. A. Porkhanov ◽

N. S. Ryabin

Keyword(s):

Risk Factors ◽

Differential Diagnosis ◽

Liver Tumors ◽

Morphological Data ◽

Malignant Neoplasms ◽

Diagnostic Efficiency ◽

Efficacy Evaluation ◽

General Group ◽

Group 2 ◽

Group 1

Objective: efficacy evaluation of the CEUS LI RADS v2017® system for differential diagnosis of liver tumors in patients with and without cirrhosis.Materials and methods. Retrospective analysis of diagnostic results of the 165 patients with liver tumors (177 nodules) was done. All patients underwent CEUS with results interpretation in accordance to the CEUS LIRADSv2017 ® criteria. Patients were divided into 2 groups based on clinical and morphological data. Group 1 included 62 patients with cirrhosis and/or CVH. Group 2 included 110 patients without risk factors for HCC.Results. Diagnostic efficiency of CEUS LI RADS v2017® for HCC identification was: group 1 – Se – 100%, Sp – 88%, Ac – 95.5%; group 2 – Se – 100%, Sp – 68.8%, Ac – 72.7%; general group Se – 100%, Sp – 72.2%, Ac – 81.4%. In the 2nd group, 21 out of 22 neoplasms, confirmed morphologically as FNH, we classified as LR 4. By applying benign character and specific contrasting patterns of FNG, they were transferred from LR 4 to LR 3. This allowed to increase sensitivity and specificity of differential diagnosis in group 2 (Se – 100%, Sp – 90.6%, Ac – 91.8%) and in general group (Se – 100%, Sp – 90.1%, Ac – 93.2%). Diagnostic efficiency of the criteria for non hepatocellular malignant neoplasms (LR M) was: group 1 – Se – 77.8%, Sp – 100%, Ac – 97%; group 2 – Se – 90%, Sp – 96.7%, Ac – 93.6%; general group- Se – 88.1%, Sp – 98.3%, Ac – 94.9%.Conclusion. Our study confirmed high accuracy of the CEUS LI RADS v2017® system in the differential diagnosis of focal liver tumors. Modification of the system (in particular, transfer of typical FNG forms from the LR 4 category) will make it possible to increase the accuracy of diagnostics by 20%. It will allow to use the LI RADS v2017® system for interpretation CEUS not only among patients with liver cirrhosis, but also in a general group without risk factors of GCC.

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