Application of methyl jasmonate postharvest maintains the quality of Nanguo pears by regulating mitochondrial energy metabolism

Background: Ureaplasma urealyticum (UU) infection is the most common cause of male infertility. Zhibai Dihuang Decoction (ZBDHD) can improve the rate of forwarding motility sperm, sperm deformity rate, seminal plasma zinc and refined berry sugar levels.Methods: The potential targets of ZBDHD are obtained from The Encyclopedia of Traditional Chinese Medicine (ETCM). Orchitis-related targets were collected from the Genecards and OMIM databases. The Cytoscape and the Database for Annotation, Visualization and Integrated Discovery (DAVID) were utilized to construct and analyzed the networks. Finally, a rat model of orchitis caused by UU infection was used to detect related indicators of mitochondrial energy metabolism using TUNEL apoptosis detection technology, loss cytometry, Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) and Western Blot.Results: A total of 795 ZBDHD targets and 242 orchitis-related targets were obtained. The “ZBDHD- orchitis PPI network” was constructed and analyzed. ZBDHD can regulate signaling pathways and biological processes related to mitochondrial energy metabolism. The results of experimental studies have shown that ZBDHD maintains the integrity of sperm mitochondrial respiratory chain function by enhancing mitochondrial Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities, promotes the synthesis of mitochondrial ATP, and improves sperm energy supply, thereby improving the motility, vitality and survival rate of sperm, and effectively improving the quality of semen in UU-infected rats (p < 0.05).Conclusion:This study discovered the multi-pathway mechanism of ZBDHD intervention in UU-induced orchitis through integrated pharmacological strategies, which provides a reference for further research on the mechanism of ZBDHD intervention in orchitis in the direction of mitochondrial energy metabolism.

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The Role of Mitochondrial Energy Metabolism in Shaping the Quality of Highbush Blueberry Fruit During Storage in Ozone-Enriched Atmosphere

Food and Bioprocess Technology ◽

10.1007/s11947-021-02696-x ◽

2021 ◽

Author(s):

Tomasz Piechowiak ◽

Patrycja Sowa ◽

Maria Tarapatskyy ◽

Maciej Balawejder

Keyword(s):

Energy Metabolism ◽

Defense Mechanisms ◽

Energy Charge ◽

Ozone Treatment ◽

Mitochondrial Energy Metabolism ◽

Antioxidative Properties ◽

Mitochondrial Ros ◽

Ros Accumulation ◽

Activity Of Enzymes

AbstractThe aim of this study was to evaluate the effect of ozone treatment on the mitochondrial energy metabolism in blueberry fruit during storage as well as to determine the relationship between the activity of mitochondria and the antioxidative properties of ozonated fruit. Blueberry fruit was stored for 28 days at 4 °C and ozonated daily with gaseous ozone at the concentration of 15 mg L−1 for 30 min, every 12 h of storage. Research showed that ozonated fruit was characterized by higher activity of enzymes involved in oxidative phosphorylation (by 58.7% for SDH, 118.2% for CCO, and 78.16% for H+-ATPase after 7 days, respectively) than non-ozonated sample, which contributed to reduction of the loss of energy charge and ATP in the fruit during storage. Moreover, the increased activity of mitochondria led to the growth of mitochondrial ROS accumulation which, in turn, activated defense mechanisms against oxidative stress in the fruit. These metabolic responses might collectively contribute to increase the antioxidative properties of ozonated fruit and consequently to maintain a good quality of the fruit over a long period of storage.

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The role of Ndufb6 subunit of the electron transport system complex I in the regulation of mitochondrial energy metabolism and insulin sensitivity in C2C12 myotubes

Diabetologie und Stoffwechsel ◽

10.1055/s-0037-1601643 ◽

2017 ◽

Vol 12 (S 01) ◽

pp. S1-S84

Author(s):

T Jelenik ◽

SW Görgens ◽

N Krako Jakovljevic ◽

I Rokitta ◽

NM Lalic ◽

...

Keyword(s):

Insulin Sensitivity ◽

Energy Metabolism ◽

Electron Transport ◽

Transport System ◽

Complex I ◽

Electron Transport System ◽

C2c12 Myotubes ◽

Mitochondrial Energy Metabolism

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Therapeutic Approaches Using Riboflavin in Mitochondrial Energy Metabolism Disorders

Current Drug Targets ◽

10.2174/1389450117666160813180812 ◽

2016 ◽

Vol 17 (13) ◽

pp. 1527-1534 ◽

Cited By ~ 18

Author(s):

Bárbara J. Henriques ◽

Tânia G. Lucas ◽

Cláudio M. Gomes

Keyword(s):

Energy Metabolism ◽

Therapeutic Approaches ◽

Mitochondrial Energy Metabolism

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Mitochondrial energy metabolism is negatively regulated by cannabinoid receptor 1 in intact human epidermis

Experimental Dermatology ◽

10.1111/exd.14110 ◽

2020 ◽

Vol 29 (7) ◽

pp. 616-622 ◽

Cited By ~ 1

Author(s):

Attila Oláh ◽

Majid Alam ◽

Jérémy Chéret ◽

Nikolett Gréta Kis ◽

Zoltán Hegyi ◽

...

Keyword(s):

Energy Metabolism ◽

Cannabinoid Receptor ◽

Human Epidermis ◽

Cannabinoid Receptor 1 ◽

Mitochondrial Energy Metabolism

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Effect of Methyl Jasmonate, Cytokinin, and Lavender Oil on Antioxidant Enzyme System of Apricot Fruit (Prunus armeniaca L.)

Sustainability ◽

10.3390/su13158565 ◽

2021 ◽

Vol 13 (15) ◽

pp. 8565

Author(s):

Seyda Cavusoglu ◽

Nurettin Yilmaz ◽

Firat Islek ◽

Onur Tekin ◽

Halil Ibrahim Sagbas ◽

...

Keyword(s):

Enzyme Activity ◽

Methyl Jasmonate ◽

Prunus Armeniaca ◽

Lavender Oil ◽

Defense Systems ◽

Antioxidant Enzyme System ◽

Prunus Armeniaca L ◽

Antioxidant Defense Systems ◽

And Storage

Various treatments are carried out in order to extend both the shelf life and storage life of fresh fruit and vegetables after harvest and among them non-toxic for humans, environmentally and economically friendly alternative treatments are gained more importance. In the current study, methyl jasmonate (MeJA), cytokinin, and lavender oil which are eco-friendly and safe for human health were applied on apricot fruit. The treated fruit were stored at 0 °C and 90–95% relative humidity for 25 days and catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX) enzyme activities and lipid peroxidation of apricots after treatments were studied. According to the findings obtained from the study, it was observed that 5 ppm cytokinin and 1000 ppm lavender oil treatments of apricot fruit gave better APX and CAT enzyme activity, respectively. In addition, better SOD enzyme activity in fruit was obtained with MeJA+lavender oil treatments. As a result, it can be emphasized that the product quality of apricot fruit is preserved as both the eco-friendly application of MeJA, cytokinin, and lavender oil separately from each other and the treatment of combinations between these compounds activate the enzymatic antioxidant defense systems of apricot fruit after harvest.

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Mitochondrial P2X7 receptor localization modulates energy metabolism enhancing physical performance

Function ◽

10.1093/function/zqab005 ◽

2021 ◽

Author(s):

Alba Clara Sarti ◽

Valentina Vultaggio-Poma ◽

Simonetta Falzoni ◽

Sonia Missiroli ◽

Anna Lisa Giuliani ◽

...

Keyword(s):

Energy Metabolism ◽

Physical Fitness ◽

P2x7 Receptor ◽

Human Fibroblasts ◽

Atp Synthesis ◽

Heart Tissue ◽

Mitochondrial Potential ◽

Mitochondrial Energy Metabolism ◽

Microglia Cell ◽

Basal Expression

Abstract Basal expression of the P2X7 receptor (P2X7R) improves mitochondrial metabolism, ATP synthesis and overall fitness of immune and non-immune cells. We investigated P2X7R contribution to energy metabolism and subcellular localization in fibroblasts (mouse embryo fibroblasts and HEK293 human fibroblasts), mouse microglia (primary brain microglia and the N13 microglia cell line), and heart tissue. The P2X7R localizes to mitochondria, and its lack a) decreases basal respiratory rate, ATP-coupled respiration, maximal uncoupled respiration, resting mitochondrial potential, mitochondrial matrix Ca2+ level, b) modifies expression pattern of oxidative phosphorylation (OxPhos) enzymes, and c) severely affects cardiac performance. Hearts from P2rx7-deleted versus WT mice are larger, heart mitochondria smaller, and stroke volume (SV), ejection fraction (EF), fractional shortening (FS) and cardiac output (CO), are significantly decreased. Accordingly, physical fitness of P2X7R-null mice is severely reduced. Thus, the P2X7R is a key modulator of mitochondrial energy metabolism and a determinant of physical fitness.

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Protective effects of lipoic acid against acrylamide-induced neurotoxicity: involvement of mitochondrial energy metabolism and autophagy

Food & Function ◽

10.1039/c7fo01429e ◽

2017 ◽

Vol 8 (12) ◽

pp. 4657-4667 ◽

Cited By ~ 11

Author(s):

Ge Song ◽

Zhigang Liu ◽

Luanfeng Wang ◽

Renjie Shi ◽

Chuanqi Chu ◽

...

Keyword(s):

Energy Metabolism ◽

Lipoic Acid ◽

Redox Status ◽

Protective Effects ◽

Mitochondrial Energy Metabolism

Lipoic acid (LA) suppressed acrylamide (ACR)-induced inflammation, redox status disturbance, autophagy, and apoptosis mediated by mitochondria in the SH-SY5Y cells.

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