scholarly journals Exploring the Mechanism of Zhibai Dihuang Decoction in the Treatment of Ureaplasma Urealyticum-Induced Orchitis Based on Integrated Pharmacology

2021 ◽  
Vol 12 ◽  
Author(s):  
Dong-hua Bin ◽  
Shi-ying Zhang ◽  
Min Zhan ◽  
Ling Li ◽  
Ying-qiu Li ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Ureaplasma Urealyticum ◽  
Experimental Studies ◽  
Mitochondrial Respiratory Chain ◽  
Mitochondrial Energy Metabolism ◽  
Reverse Transcription Pcr ◽  
Detection Technology ◽  
Apoptosis Detection ◽  
Sugar Levels

Background: Ureaplasma urealyticum (UU) infection is the most common cause of male infertility. Zhibai Dihuang Decoction (ZBDHD) can improve the rate of forwarding motility sperm, sperm deformity rate, seminal plasma zinc and refined berry sugar levels.Methods: The potential targets of ZBDHD are obtained from The Encyclopedia of Traditional Chinese Medicine (ETCM). Orchitis-related targets were collected from the Genecards and OMIM databases. The Cytoscape and the Database for Annotation, Visualization and Integrated Discovery (DAVID) were utilized to construct and analyzed the networks. Finally, a rat model of orchitis caused by UU infection was used to detect related indicators of mitochondrial energy metabolism using TUNEL apoptosis detection technology, loss cytometry, Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) and Western Blot.Results: A total of 795 ZBDHD targets and 242 orchitis-related targets were obtained. The “ZBDHD- orchitis PPI network” was constructed and analyzed. ZBDHD can regulate signaling pathways and biological processes related to mitochondrial energy metabolism. The results of experimental studies have shown that ZBDHD maintains the integrity of sperm mitochondrial respiratory chain function by enhancing mitochondrial Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities, promotes the synthesis of mitochondrial ATP, and improves sperm energy supply, thereby improving the motility, vitality and survival rate of sperm, and effectively improving the quality of semen in UU-infected rats (p < 0.05).Conclusion:This study discovered the multi-pathway mechanism of ZBDHD intervention in UU-induced orchitis through integrated pharmacological strategies, which provides a reference for further research on the mechanism of ZBDHD intervention in orchitis in the direction of mitochondrial energy metabolism.

scholarly journals Application of methyl jasmonate postharvest maintains the quality of Nanguo pears by regulating mitochondrial energy metabolism

2021 ◽  
Vol 20 (11) ◽  
pp. 3075-3083
Author(s):  
Can-ying LI ◽  
Yuan CHENG ◽  
Jia-bao HOU ◽  
Jie ZHU ◽  
Lei SUN ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Methyl Jasmonate ◽  
Mitochondrial Energy Metabolism

scholarly journals The Role of Mitochondrial Energy Metabolism in Shaping the Quality of Highbush Blueberry Fruit During Storage in Ozone-Enriched Atmosphere

2021 ◽  
Author(s):  
Tomasz Piechowiak ◽  
Patrycja Sowa ◽  
Maria Tarapatskyy ◽  
Maciej Balawejder
Keyword(s):  
Energy Metabolism ◽  
Defense Mechanisms ◽  
Energy Charge ◽  
Ozone Treatment ◽  
Mitochondrial Energy Metabolism ◽  
Antioxidative Properties ◽  
Mitochondrial Ros ◽  
Ros Accumulation ◽  
Activity Of Enzymes

AbstractThe aim of this study was to evaluate the effect of ozone treatment on the mitochondrial energy metabolism in blueberry fruit during storage as well as to determine the relationship between the activity of mitochondria and the antioxidative properties of ozonated fruit. Blueberry fruit was stored for 28 days at 4 °C and ozonated daily with gaseous ozone at the concentration of 15 mg L−1 for 30 min, every 12 h of storage. Research showed that ozonated fruit was characterized by higher activity of enzymes involved in oxidative phosphorylation (by 58.7% for SDH, 118.2% for CCO, and 78.16% for H+-ATPase after 7 days, respectively) than non-ozonated sample, which contributed to reduction of the loss of energy charge and ATP in the fruit during storage. Moreover, the increased activity of mitochondria led to the growth of mitochondrial ROS accumulation which, in turn, activated defense mechanisms against oxidative stress in the fruit. These metabolic responses might collectively contribute to increase the antioxidative properties of ozonated fruit and consequently to maintain a good quality of the fruit over a long period of storage.

The system of indicators of the quality of the carbon-carbon composite materials and technologies of their production

2018 ◽  
pp. 127-132
Author(s):  
T. N. Antipova ◽  
D. S. Shiroyan
Keyword(s):  
Composite Material ◽  
Low Cost ◽  
Experimental Studies ◽  
Carbon Matrix ◽  
Carbon Composite ◽  
Optimal Number ◽  
Laboratory Equipment ◽  
Carbon Composite Material ◽  
Carbon Composite Materials

The system of indicators of quality of carbon-carbon composite material and technological operations of its production is proved in the work. As a result of the experimental studies, with respect to the existing laboratory equipment, the optimal number of cycles of saturation of the reinforcing frame with a carbon matrix is determined. It was found that to obtain a carbon-carbon composite material with a low cost and the required quality indicators, it is necessary to introduce additional parameters of the pitch melt at the impregnation stage.

Therapeutic Approaches Using Riboflavin in Mitochondrial Energy Metabolism Disorders

2016 ◽  
Vol 17 (13) ◽  
pp. 1527-1534 ◽  
Author(s):  
Bárbara J. Henriques ◽  
Tânia G. Lucas ◽  
Cláudio M. Gomes
Keyword(s):  
Energy Metabolism ◽  
Therapeutic Approaches ◽  
Mitochondrial Energy Metabolism

Mitochondrial energy metabolism is negatively regulated by cannabinoid receptor 1 in intact human epidermis

2020 ◽  
Vol 29 (7) ◽  
pp. 616-622 ◽  
Author(s):  
Attila Oláh ◽  
Majid Alam ◽  
Jérémy Chéret ◽  
Nikolett Gréta Kis ◽  
Zoltán Hegyi ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Cannabinoid Receptor ◽  
Human Epidermis ◽  
Cannabinoid Receptor 1 ◽  
Mitochondrial Energy Metabolism

scholarly journals Nondestructive Detection of the Gel State of Preserved Eggs Based on Dielectric Impedance

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 394
Author(s):  
Cheng-Han Li ◽  
Chun-Hung Hsieh ◽  
Cheng-Chu Hung ◽  
Ching-Wei Cheng
Keyword(s):  
Decay Rate ◽  
Mechanical Vibration ◽  
Scientific Basis ◽  
Nondestructive Method ◽  
Inspection System ◽  
Nyquist Diagram ◽  
Detection Technology ◽  
The Difference ◽  
The Individual

After completing the production of preserved eggs, traditionally, the degree of gelling is judged by allowing workers to tap the preserved eggs with their fingers and sense the resulting oscillations. The amount of oscillation is used for the quality classification. This traditional method produces varying results owing to the differences in the sensitivity of the individual workers, who are not objective. In this study, dielectric detection technology was used to classify the preserved eggs nondestructively. The impedance in the frequency range of 2–300 kHz was resolved into resistance and reactance, and was plotted on a Nyquist diagram. Next, the diagram curve was fitted in order to obtain the equivalent circuit, and the difference in the compositions of the equivalent circuits corresponding to gelled and non-gelled preserved eggs was analyzed. A preserved egg can be considered an RLC series circuit, and its decay rate is consistent with the decay rate given by mechanical vibration theory. The Nyquist diagrams for the resistance and reactance of preserved eggs clearly showed that the resistance and reactance of gelled and non-gelled eggs were quite different, and the classification of the eggs was performed using Bayesian network (BN). The results showed that a BN classifier with two variables, i.e., resistance and reactance, can be used to classify preserved eggs as gelled or non-gelled, with an accuracy of 81.0% and a kappa value of 0.62. Thus, a BN classifier based on resistance and reactance demonstrates the ability to classify the quality of preserved egg gel. This research provides a nondestructive method for the inspection of the quality of preserved egg gel, and provides a theoretical basis for the development of an automated preserved egg inspection system that can be used as the scientific basis for the determination of the quality of preserved eggs.

scholarly journals Mitochondrial P2X7 receptor localization modulates energy metabolism enhancing physical performance

Function ◽  
2021 ◽  
Author(s):  
Alba Clara Sarti ◽  
Valentina Vultaggio-Poma ◽  
Simonetta Falzoni ◽  
Sonia Missiroli ◽  
Anna Lisa Giuliani ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Physical Fitness ◽  
P2x7 Receptor ◽  
Human Fibroblasts ◽  
Atp Synthesis ◽  
Heart Tissue ◽  
Mitochondrial Potential ◽  
Mitochondrial Energy Metabolism ◽  
Microglia Cell ◽  
Basal Expression

Abstract Basal expression of the P2X7 receptor (P2X7R) improves mitochondrial metabolism, ATP synthesis and overall fitness of immune and non-immune cells. We investigated P2X7R contribution to energy metabolism and subcellular localization in fibroblasts (mouse embryo fibroblasts and HEK293 human fibroblasts), mouse microglia (primary brain microglia and the N13 microglia cell line), and heart tissue. The P2X7R localizes to mitochondria, and its lack a) decreases basal respiratory rate, ATP-coupled respiration, maximal uncoupled respiration, resting mitochondrial potential, mitochondrial matrix Ca2+ level, b) modifies expression pattern of oxidative phosphorylation (OxPhos) enzymes, and c) severely affects cardiac performance. Hearts from P2rx7-deleted versus WT mice are larger, heart mitochondria smaller, and stroke volume (SV), ejection fraction (EF), fractional shortening (FS) and cardiac output (CO), are significantly decreased. Accordingly, physical fitness of P2X7R-null mice is severely reduced. Thus, the P2X7R is a key modulator of mitochondrial energy metabolism and a determinant of physical fitness.

Protective effects of lipoic acid against acrylamide-induced neurotoxicity: involvement of mitochondrial energy metabolism and autophagy

2017 ◽  
Vol 8 (12) ◽  
pp. 4657-4667 ◽  
Author(s):  
Ge Song ◽  
Zhigang Liu ◽  
Luanfeng Wang ◽  
Renjie Shi ◽  
Chuanqi Chu ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Lipoic Acid ◽  
Redox Status ◽  
Protective Effects ◽  
Mitochondrial Energy Metabolism

Lipoic acid (LA) suppressed acrylamide (ACR)-induced inflammation, redox status disturbance, autophagy, and apoptosis mediated by mitochondria in the SH-SY5Y cells.

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