The concentration of strontium and other minerals in animal feed ingredients

2013 ◽  
Vol 2 ◽  
Author(s):  
L.C. Browning ◽  
A.J. Cowieson
Keyword(s):  
Inductively Coupled Plasma ◽  
Animal Feed ◽  
Optical Emission ◽  
Grain Legume ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
High Concentration ◽  
Mineral Concentration ◽  
Feed Ingredients ◽  
Cereal Grain

SummaryVariance in macro- and micro-mineral concentration in feed ingredients for farmed livestock contributes to sub-optimal performance and may compromise health and welfare. Although routine quality assurance and quality control procedures in feed mills or integrated poultry or swine businesses may track variance in the concentration of minerals of immediate nutritional importance, such as phosphorus (P), calcium (Ca) and sodium (Na), micro-minerals such as strontium (Sr) attract less attention. In order to create a framework for further study, the mineral concentration in more than 130 animal feed ingredients commonly used in Australia were analysed by inductively coupled plasma optical emission spectroscopy (ICP-OES). Due to a dearth of information, the principal focus of the survey was Sr, but the concentration of Ca, P, magnesium (Mg), manganese (Mn), potassium (K), iron (Fe), copper (Cu), zinc (Zn), sulphur (S) and Na were analysed concurrently. Generally the minerals present at the highest concentrations in the various feed ingredients examined were Ca, P and Mg. As anticipated, the ingredients with the highest concentrations of Ca and P were inorganic phosphates, limestone and meat and bone meal. The average Ca concentration in limestone was 393 g/kg but a range of 376–415 g/kg was observed which may be nutritionally important. Furthermore, the Mg concentration in limestone ranged from 7–535 mg/kg suggesting some contamination by dolomite lime sources. A total of 24 meat and bone meal samples were included in the analysis and mean Ca and P concentrations were 109 and 54 g/kg respectively. However, the range of Ca and P in meat and bone meal was considerable with Ca concentrations from 51–148 g/kg and P concentrations from 26–66 g/kg. A total of 81 cereal, grain legume and cereal by-product samples were included as part of the survey and these vegetable feed ingredients contained relatively low concentrations of most minerals with Ca, P, Mg and K dominating. The K concentration of soybean meal was found to be around 23 g/kg and ranged from approximately 22–27 g/kg. In comparison, the Sr concentration in the feed ingredients was low relative to other minerals, with limestone having the highest level of strontium at 329 mg/kg. Overall those feed ingredients from a mineral origin had the highest level of Sr. In addition, meat and bone meal had a relatively high concentration of Sr (around 159 mg/kg).

Advances in sample digestion using microwave-ultraviolet radiations: phosphorus and sulfur determination in animal feed

2020 ◽  
Vol 16 ◽  
Author(s):  
Diogo L. R. Novo ◽  
Priscila T. Scaglioni ◽  
Rodrigo M. Pereira ◽  
Filipe S. Rondan ◽  
Gilberto S. Coelho Junior ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Animal Feed ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
Official Method ◽  
Dissolved Carbon ◽  
Inductively Coupled ◽  
Relative Standard ◽  
Wide Range ◽  
Sulfur Determination

Background: Conventional analytical methods for phosphorus and sulfur determination in several matrices present normally analytical challenges regarding inaccuracy, detectability and waste generation. Objective: The main objective is proposing a green and feasible analytical method for phosphorus and sulfur determination in animal feed. Methods: Synergic effect between microwave and ultraviolet radiations during sample preparation was evaluated for the first time for the animal feed digestion associated with further phosphorus and sulfur determination by ion chromatography with conductivity detection. Dissolved carbon and residual acidity in final digests were used for the proposed method assessment. Phosphorus and sulfur values were compared with those obtained using conventional microwave-assisted wet digestion in closed vessels associated with inductively coupled plasma optical emission spectrometry and with those obtained using Association of Official Analytical Chemists International official method. Recovery tests and certified reference material analysis were performed. Animal feeds were analyzed using the proposed method. Results: Sample masses of 500 mg were efficiently digested using only 2 mol L -1 HNO3. The results obtained by the proposed method was not differing significantly (p > 0.05) from those obtained by the conventional and official methods. Suitable recoveries (from 94 to 99%), agreement with certified values (101 and 104%) and relative standard deviations (< 8%) were achieved. Phosphorus and sulfur content in commercial products varied in a wide range (P: 5,873 to 28,387 mg kg-1 and S: 2,165 to 4,501 mg kg-1 ). Conclusion: The proposed method is a green, safe, accurate, precise and sensitive alternative for animal feed quality control.

Mycological Survey of Chicken Feed and Some Feed Ingredients in Turkey

1988 ◽  
Vol 51 (10) ◽  
pp. 807-810 ◽  
Author(s):  
DILEK HEPERKAN ◽  
ÍHSAN ALPERDEN
Keyword(s):  
Fish Meal ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Mixed Feed ◽  
Feed Ingredients ◽  
Penicillium Aurantiogriseum ◽  
Sunflower Cake ◽  
Feed Samples ◽  
Mold Count ◽  
Meat Bone Meal

Level of mold contamination and mycoflora were determined for 144 mixed feed and feed ingredients, including corn, sunflower cake, soja cake, meat and bone meal, and fish meal. Four samples were found to be free of mold. Among the feed samples examined, the mold count has been found to be low (102 to 103 colonies/g) for fish meal, high (104 to 105 colonies/g) for meat-bone meal and sunflower cake, and extremely high (more than 105 colonies/g) for soja cake, corn and mixed feed. The predominant flora in the feed samples consisted of Penicillium, Aspergillus, Fusarium, Mucor and Eurotium, respectively. The most frequently encountered species was found to be Penicillium aurantiogriseum, followed by Aspergillus flavus.

Validation of a PCR-Based Method for the Detection of Various Rendered Materials in Feedstuffs Using a Forensic DNA Extraction Kit

2006 ◽  
Vol 69 (1) ◽  
pp. 205-210 ◽  
Author(s):  
MICHAEL J. MYERS ◽  
HAILE F. YANCY ◽  
MICHAEL ARANETA ◽  
JENNIFER ARMOUR ◽  
JANICE DERR ◽  
...  
Keyword(s):  
False Positive ◽  
Animal Feed ◽  
Limit Of Detection ◽  
False Positive Rate ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Average Accuracy ◽  
Positive Rate ◽  
Feed Samples ◽  
Pcr Primer

A method trial was initiated to validate the use of a commercial DNA forensic kit to extract DNA from animal feed as part of a PCR-based method. Four different PCR primer pairs (one bovine pair, one porcine pair, one ovine primer pair, and one multispecies pair) were also evaluated. Each laboratory was required to analyze a total of 120 dairy feed samples either not fortified (control, true negative) or fortified with bovine meat and bone meal, porcine meat and bone meal (PMBM), or lamb meal. Feeds were fortified with the animal meals at a concentration of 0.1% (wt/wt). Ten laboratories participated in this trial, and each laboratory was required to evaluate two different primer pairs, i.e., each PCR primer pair was evaluated by five different laboratories. The method was considered to be validated for a given animal source when three or more laboratories achieved at least 97% accuracy (29 correct of 30 samples for 96.7% accuracy, rounded up to 97%) in detecting the fortified samples for that source. Using this criterion, the method was validated for the bovine primer because three laboratories met the criterion, with an average accuracy of 98.9%. The average false-positive rate was 3.0% in these laboratories. A fourth laboratory was 80% accurate in identifying the samples fortified with bovine meat and bone meal. A fifth laboratory was not able to consistently extract the DNA from the feed samples and did not achieve the criterion for accuracy for either the bovine or multispecies PCR primers. For the porcine primers, the method was validated, with four laboratories meeting the criterion for accuracy with an average accuracy of 99.2%. The fifth laboratory had a 93.3% accuracy outcome for the porcine primer. Collectively, these five laboratories had a 1.3% false-positive rate for the porcine primer. No laboratory was able to meet the criterion for accuracy with the ovine primers, most likely because of problems with the synthesis of the primer pair; none of the positive control DNA samples could be detected with the ovine primers. The multispecies primer pair was validated in three laboratories for use with bovine meat and bone meal and lamb meal but not with PMBM. The three laboratories had an average accuracy of 98.9% for bovine meat and bone meal, 97.8% for lamb meal, and 63.3% for PMBM. When examined on an individual laboratory basis, one of these four laboratories could not identify a single feed sample containing PMBM by using the multispecies primer, whereas the other laboratory identified only one PMBM-fortified sample, suggesting that the limit of detection for PMBM with this primer pair is around 0.1% (wt/wt). The results of this study demonstrated that the DNA forensic kit can be used to extract DNA from animal feed, which can then be used for PCR analysis to detect animal-derived protein present in the feed sample.

scholarly journals Meat and bone meal still used in animal feed

Nature ◽  
2001 ◽  
Vol 414 (6860) ◽  
pp. 147-147
Author(s):  
Stephen Rossides
Keyword(s):  
Animal Feed ◽  
Meat And Bone Meal ◽  
Bone Meal

scholarly journals Crystallization of CaCO3 in Aqueous Solutions with Extremely High Concentrations of NaCl

Crystals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 647
Author(s):  
Mengqi Qian ◽  
Yuwei Zuo ◽  
Zhihao Chen ◽  
Xiaoshuang Yin ◽  
Ying Liu ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
High Concentration ◽  
Retention Efficiency ◽  
Static Test ◽  
Inductively Coupled ◽  
High Concentrations ◽  
The One ◽  
Phosphate Inhibitors

The effect of NaCl at extremely high concentrations from 3.5 to 14 wt. % on the crystallization of CaCO3 was investigated in depth. The static test experiment verified that the Ca2+ retention efficiency (η) of NaCl on CaCO3 scale increased from 31.06% (3.5 wt. %) to 41.56% (14 wt. %). Based on the calculation of supersaturation rations, the high concentration of NaCl could reduce the activity coefficients of [Ca2+] and [CO32−], thus reducing the actual concentration of CaCO3. The CaCO3 deposition rate constants (k) showed that NaCl slowed down the rate of CaCO3 crystallization. The X–ray diffraction (XRD) testing disclosed that the growth of (1 0 4) and (1 1 0) faces from calcite was impeded, while the formation of (1 1 1) face from aragonite was induced by the increasing concentration of NaCl. The inductively coupled plasma optical emission spectrometry (ICP–OES) results indicated that Na+ could be doped into CaCO3, leading to the one–dimensional crystal growth. It was further proved that NaCl heightens the efficiency of the typical phosphate inhibitors (2–phosphonobutane–1,2,4–tricarboxylic acid (PBTCA) and 1–hydroxyethane–1,1–diphosphonic acid (HEDP)) on prohibiting the scale of CaCO3.

Detection of Rendered Meat and Bone Meals by PCR Is Dependent on Animal Species of Origin and DNA Extraction Method

2010 ◽  
Vol 73 (6) ◽  
pp. 1090-1096 ◽  
Author(s):  
MICHAEL J. MYERS ◽  
DOROTHY E. FARRELL ◽  
CHRISTINE M. DEAVER ◽  
JACQULINE MASON ◽  
HEIDI L. SWAIM ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Extraction ◽  
Real Time Pcr ◽  
Animal Species ◽  
Animal Feed ◽  
Functional Assay ◽  
Threshold Values ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Pcr Method

The capability of eight commercially available DNA extraction kits to extract bovine DNA originating in meat and bone meal from fortified feed was evaluated. Four different batches of bovine meat and bone meal (BMBM) were used for DNA extraction with the eight commercial DNA extraction kits. Within each kit, there were minimal differences in the batch-to-batch amounts of extracted DNA. There were differences between the kits in the amounts of DNA that could be extracted from the same amount of starting BMBM. These differences did not translate into differences in the amount of amplifiable DNA from BMBM-fortified dairy feed. Using a validated real-time PCR method, the kit yielding the highest amount extractable DNA was completely unable to yield a positive PCR result; one other kit was also unable to produce a positive PCR result from DNA extracted from BMBM-fortified feed. There was a complete lack of a correlation between the amount of bovine DNA isolated from BMBM by a given extraction kit compared with the relative amounts of DNA isolated from fortified animal feed as evidenced by the cycle threshold values generated using the real-time PCR method. These results demonstrate that extraction of DNA from processed animal protein is different for pure ingredients and fortified animal feeds. These results indicate that a method specifically developed using just animal-derived meat and bone meal may not yield a functional assay when used to detect animal tissues in complete animal feed.

scholarly journals In vitro surface biocompatibility of high-content silicon-substituted calcium phosphate ceramics

2013 ◽  
Vol 11 (2) ◽  
pp. 140-150 ◽  
Author(s):  
Nasser Mostafa ◽  
Abdallah Shaltout ◽  
Lachezar Radev ◽  
Hassan Hassan
Keyword(s):  
Calcium Phosphate ◽  
Inductively Coupled Plasma ◽  
Energy Dispersive Spectrometer ◽  
Optical Emission ◽  
Ionic Concentration ◽  
Apatite Layer ◽  
High Concentration ◽  
Calcium Phosphate Ceramics ◽  
Porous Microstructure

AbstractThe present work investigates surface biocompatibility of silicon-substituted calcium phosphate ceramics. Different silicon-substituted calcium phosphate ceramic bodies were prepared from co-precipitated powders by sintering at 1300°C. The in vitro bioactivity of the ceramics was assessed in simulated body fluid (SBF) at 37°C for periods up to 4 weeks. The changes in the surface morphology and composition were determined by scanning electron microscopy (SEM) coupled with electron probe microanalysis and energy dispersive spectrometer (EDX). Inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to observe the change in ionic concentration of SBF after removal of the samples. The bioactivity of the ceramics increased with an increasing silicate ion substitution in a systematic way. The surface of ceramics with 2.23% silicon substitution was partially covered with apatite layer after one week, while ceramics with 8.1% silicon substitution were completely covered with apatite in the first week. The porous microstructure of high-concentration Si-substituted ceramics helps the dissolution of surface ions and the leaching process. This allows SBF to reach supersaturation in a short time and accelerate the deposition of apatite layer.

Production of Monoclonal Antibody for the Detection of Meat and Bone Meal in Animal Feed

2004 ◽  
Vol 52 (25) ◽  
pp. 7580-7585 ◽  
Author(s):  
Shin-Hee Kim ◽  
Tung-Shi Huang ◽  
Thomas A. Seymour ◽  
Cheng-i Wei ◽  
Stephen C. Kempf ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Animal Feed ◽  
Meat And Bone Meal ◽  
Bone Meal

Development of Immunoassay for Detection of Meat and Bone Meal in Animal Feed

2005 ◽  
Vol 68 (9) ◽  
pp. 1860-1865 ◽  
Author(s):  
SHIN-HEE KIM ◽  
TUNG-SHI HUANG ◽  
THOMAS A. SEYMOUR ◽  
CHENG-I WEI ◽  
STEPHEN C. KEMPF ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Monoclonal Antibodies ◽  
Animal Feed ◽  
Superior Performance ◽  
Hybridoma Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Efficient Detection ◽  
Heat Treated

An immunoassay system was developed for efficient detection of prohibited meat and bone meal (MBM) in animal feed. Monoclonal antibodies (MAbs) were raised against bovine smooth muscle autoclaved at 130°C for 20 min. Among the 1,500 supernatants of hybridoma cells screened, MAbs 3E1, 1G3, and 3E10 were selected and characterized in this study. The first set of MAbs produced, 3E1 and 1G3, had stronger reactivity against MBM than against smooth muscle that was heat treated at 90°C for 10 min. However, reactivity gradually increased against smooth muscle that was autoclaved at 130°C for up to 1 h. The enzyme-linked immunosorbent assay for detection of MBM in animal feed was optimized with the MAb 3E10 because of its superior performance. MAb 3E10 diluted to 100-fold was used to differentiate bovine MBM from that of other species in ingredients used for commercial animal feeds and could detect down to 0.05% MBM mixed in animal feed.

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