Reevaluating Diet and Technology in the Archaic Great Basin Using Starch Grain Assemblages from Hogup Cave UTAH

2016 ◽  
Vol 81 (4) ◽  
pp. 664-681
Author(s):  
Nicole M. Herzog ◽  
Anne T. Lawlor
Keyword(s):  
Great Basin ◽  
Direct Evidence ◽  
Early Holocene ◽  
Timing Constraints ◽  
Starch Granules ◽  
Plant Foods ◽  
Relative Value ◽  
Dietary Transition ◽  
Dietary Shifts

Identifying the relationships between prehistoric technologies and their role in food processing is important in understanding the timing, constraints, and motivations for dietary shifts in the past. However, direct evidence linking tools to specific plant foods is often lacking. This is especially true for archaeological assemblages dating to the early Holocene in the Great Basin. During this period, the appearance of milling stones and coiled basketry has often been assumed to represent a dietary transition marked by an increased reliance on lower-return plant foods, specifically small seeds. Here we evaluate the role of milling stones and basketry in the processing of low-return seeds by analyzing starch granules recovered from artifacts dating to the early and mid-Holocene from Hogup Cave, Utah. Results suggest that basketry and milling stones were used to process different resource types. Starch granules on milling stones primarily represent geophytes, while those from baskets are limited to small-seeded plant taxa mainly within the amaranth family. Results lead us to reevaluate the hypothesis that the appearance of these tools signals dietary broadening and prompt an array of new questions about early Holocene diets, the role and costs of processing technology, and the relative value of seed resources.

scholarly journals The Role of Basketry in Early Holocene Small Seed Exploitation: Implications of a Ca. 9,000 Year-Old Basket from Cowboy Cave, Utah

2008 ◽  
Vol 73 (1) ◽  
pp. 83-102 ◽  
Author(s):  
Phil R. Geib ◽  
Edward A. Jolie
Keyword(s):  
North America ◽  
Great Basin ◽  
Processing Technique ◽  
Early Holocene ◽  
Western North America ◽  
Seed Processing ◽  
Small Seed ◽  
Food Exploitation ◽  
The Continuum

Despite ranking at the low end of the continuum in net caloric benefit relative to other foods, small seeds assumed great dietary importance in many parts of the world, including western North America. In a series of publications, Adovasio (1970a, 1974, 1980, 1986) argued that coiled basketry technology was invented in the eastern Great Basin during the early Holocene as a specialized food-processing technique. Coiled baskets are indeed useful for collecting and processing seeds, but it does not necessarily follow that they were originally designed for this purpose. A whole basket recently discovered at Cowboy Cave in southeastern Utah returned an AMS radiocarbon assay of 7960 ± 50 B.P., making it currently the earliest directly dated coiled basket from the Americas. This basket is not a parching tray and likely had nothing to do with harvesting seeds. We discuss the implications of this find with regard to tracking the temporal spread of coiled basketry technology in western North America and the role of coiled and twined forms in the initiation of small seed exploitation. Coiled and twined baskets for small seed processing may result from reconfiguration of existing technologies to create novel forms suited to a new food exploitation strategy.

scholarly journals Younger Dryas and early Holocene subsistence in the northern Great Basin: multiproxy analysis of coprolites from the Paisley Caves, Oregon, USA

2020 ◽  
Vol 12 (9) ◽  
Author(s):  
John C. Blong ◽  
Martin E. Adams ◽  
Gabriel Sanchez ◽  
Dennis L. Jenkins ◽  
Ian D. Bull ◽  
...  
Keyword(s):  
Land Use ◽  
Great Basin ◽  
Direct Evidence ◽  
Small Animal ◽  
Younger Dryas ◽  
Early Holocene ◽  
Land Use Patterns ◽  
Human Diet ◽  
Valley Bottom ◽  
Western Stemmed Tradition

Abstract Younger Dryas and early Holocene Western Stemmed Tradition occupants of the northern Great Basin appear to have practiced a broad-based subsistence strategy including the consumption of a wide variety of small animal and plant resources. However, much of our evidence for human diet and land use during this period comes from dry cave and rockshelter sites where it can be challenging to distinguish plant and small animal remains deposited as a result of human versus nonhuman activity. This study presents new direct evidence for Younger Dryas and early Holocene human diet in the northern Great Basin through multiproxy analysis of nine human coprolites from the Paisley Caves, Oregon, USA. The evidence indicates that Western Stemmed Tradition occupants consumed plants, small mammals, fish, and insects, including direct evidence for consumption of whole rodents and several types of beetle. Occupation of the caves occurred during the summer and fall by individuals foraging on wetland, sagebrush grassland, and riparian ecological landscapes suggesting geographical and seasonal variability in land-use patterns during the Younger Dryas and early Holocene periods. This research suggests that Western Stemmed Tradition settlement patterns were seasonally centered on productive valley bottom lakes and wetlands but also included forays to a variety of ecological landscapes. The results highlight the importance of plant and small animal resources in the human diet during the terminal Pleistocene settlement of North America and contribute to debates about the process of the peopling of the Americas.

Direct Evidence for the Role of Inhibition in Resolving Interference

2009 ◽  
Author(s):  
M. Karl Healey ◽  
Karen L. Campbell ◽  
Lynn Hasher ◽  
Lynn Ossher
Keyword(s):  
Direct Evidence

scholarly journals CHOP-Dependent Stress-Inducible Expression of a Novel Form of Carbonic Anhydrase VI

1999 ◽  
Vol 19 (1) ◽  
pp. 495-504 ◽  
Author(s):  
John Sok ◽  
Xiao-Zhong Wang ◽  
Nikoleta Batchvarova ◽  
Masahiko Kuroda ◽  
Heather Harding ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Target Gene ◽  
Target Genes ◽  
Direct Evidence ◽  
Nuclear Protein ◽  
Intracellular Form ◽  
Carbonic Anhydrase Vi ◽  
Responsive Element

ABSTRACT CHOP (also called GADD153) is a stress-inducible nuclear protein that dimerizes with members of the C/EBP family of transcription factors and was initially identified as an inhibitor of C/EBP binding to classic C/EBP target genes. Subsequent experiments suggested a role for CHOP-C/EBP heterodimers in positively regulating gene expression; however, direct evidence that this is the case has so far not been uncovered. Here we describe the identification of a positively regulated direct CHOP-C/EBP target gene, that encoding murine carbonic anhydrase VI (CA-VI). The stress-inducible form of the gene is expressed from an internal promoter and encodes a novel intracellular form of what is normally a secreted protein. Stress-induced expression of CA-VI is both CHOP and C/EBPβ dependent in that it does not occur in cells deficient in either gene. A CHOP-responsive element was mapped to the inducibleCA-VI promoter, and in vitro footprinting revealed binding of CHOP-C/EBP heterodimers to that site. Rescue of CA-VIexpression in c/ebpβ−/− cells by exogenous C/EBPβ and a shorter, normally inhibitory isoform of the protein known as LIP suggests that the role of the C/EBP partner is limited to targeting the CHOP-containing heterodimer to the response element and points to a preeminent role for CHOP in CA-VI induction during stress.

scholarly journals Stoichiometric Analysis of Shifting in Subcellular Compartmentalization of HSP70 within Ischemic Penumbra

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3578
Author(s):  
Federica Mastroiacovo ◽  
Francesca Biagioni ◽  
Paola Lenzi ◽  
Larisa Ryskalin ◽  
Stefano Puglisi-Allegra ◽  
...  
Keyword(s):  
Direct Evidence ◽  
Neuronal Survival ◽  
Hsp 70 ◽  
Preclinical Models ◽  
Brain Areas ◽  
Cell Compartments ◽  
Control Brain ◽  
Disease Modifier ◽  
Subcellular Compartmentalization

The heat shock protein (HSP) 70 is considered the main hallmark in preclinical studies to stain the peri-infarct region defined area penumbra in preclinical models of brain ischemia. This protein is also considered as a potential disease modifier, which may improve the outcome of ischemic damage. In fact, the molecule HSP70 acts as a chaperonine being able to impact at several level the homeostasis of neurons. Despite being used routinely to stain area penumbra in light microscopy, the subcellular placement of this protein within area penumbra neurons, to our knowledge, remains undefined. This is key mostly when considering studies aimed at deciphering the functional role of this protein as a determinant of neuronal survival. The general subcellular placement of HSP70 was grossly reported in studies using confocal microscopy, although no direct visualization of this molecule at electron microscopy was carried out. The present study aims to provide a direct evidence of HSP70 within various subcellular compartments. In detail, by using ultrastructural morphometry to quantify HSP70 stoichiometrically detected by immuno-gold within specific organelles we could compare the compartmentalization of the molecule within area penumbra compared with control brain areas. The study indicates that two cell compartments in control conditions own a high density of HSP70, cytosolic vacuoles and mitochondria. In these organelles, HSP70 is present in amount exceeding several-fold the presence in the cytosol. Remarkably, within area penumbra a loss of such a specific polarization is documented. This leads to the depletion of HSP70 from mitochondria and mostly cell vacuoles. Such an effect is expected to lead to significant variations in the ability of HSP70 to exert its physiological roles. The present findings, beyond defining the neuronal compartmentalization of HSP70 within area penumbra may lead to a better comprehension of its beneficial/detrimental role in promoting neuronal survival.

scholarly journals Expression of lysophosphatidic acid receptor 5 is necessary for the regulation of intestinal Na+/H+ exchanger 3 by lysophosphatidic acid in vivo

2018 ◽  
Vol 315 (4) ◽  
pp. G433-G442 ◽  
Author(s):  
Kayte A. Jenkin ◽  
Peijian He ◽  
C. Chris Yun
Keyword(s):  
Epithelial Cells ◽  
Lysophosphatidic Acid ◽  
Direct Evidence ◽  
Intestinal Epithelial Cells ◽  
Regulatory Role ◽  
Intestinal Epithelial ◽  
Fluid Absorption ◽  
Wild Type

Lysophosphatidic acid (LPA) is a bioactive lipid molecule, which regulates a broad range of pathophysiological processes. Recent studies have demonstrated that LPA modulates electrolyte flux in the intestine, and its potential as an antidiarrheal agent has been suggested. Of six LPA receptors, LPA5 is highly expressed in the intestine. Recent studies by our group have demonstrated activation of Na+/H+ exchanger 3 (NHE3) by LPA5. However, much of what has been elucidated was achieved using colonic cell lines that were transfected to express LPA5. In the current study, we engineered a mouse that lacks LPA5 in intestinal epithelial cells, Lpar5ΔIEC, and investigated the role of LPA5 in NHE3 regulation and fluid absorption in vivo. The intestine of Lpar5ΔIEC mice appeared morphologically normal, and the stool frequency and fecal water content were unchanged compared with wild-type mice. Basal rates of NHE3 activity and fluid absorption and total NHE3 expression were not changed in Lpar5ΔIEC mice. However, LPA did not activate NHE3 activity or fluid absorption in Lpar5ΔIEC mice, providing direct evidence for the regulatory role of LPA5. NHE3 activation involves trafficking of NHE3 from the terminal web to microvilli, and this mobilization of NHE3 by LPA was abolished in Lpar5ΔIEC mice. Dysregulation of NHE3 was specific to LPA, and insulin and cholera toxin were able to stimulate and inhibit NHE3, respectively, in both wild-type and Lpar5ΔIEC mice. The current study for the first time demonstrates the necessity of LPA5 in LPA-mediated stimulation of NHE3 in vivo. NEW & NOTEWORTHY This study is the first to assess the role of LPA5 in NHE3 regulation and fluid absorption in vivo using a mouse that lacks LPA5 in intestinal epithelial cells, Lpar5ΔIEC. Basal rates of NHE3 activity and fluid absorption, and total NHE3 expression were not changed in Lpar5ΔIEC mice. However, LPA did not activate NHE3 activity or fluid absorption in Lpar5ΔIEC mice, providing direct evidence for the regulatory role of LPA5.

scholarly journals Functional interaction between dynein light chain and intermediate chain is required for mitotic spindle positioning

2011 ◽  
Vol 22 (15) ◽  
pp. 2690-2701 ◽  
Author(s):  
Melissa D. Stuchell-Brereton ◽  
Amanda Siglin ◽  
Jun Li ◽  
Jeffrey K. Moore ◽  
Shubbir Ahmed ◽  
...  
Keyword(s):  
Light Chain ◽  
Direct Evidence ◽  
Retrograde Transport ◽  
Cytoplasmic Dynein ◽  
Dynein Light Chain ◽  
Spindle Positioning ◽  
Dynein Motor ◽  
Intermediate Chains ◽  
Activator Complex

Cytoplasmic dynein is a large multisubunit complex involved in retrograde transport and the positioning of various organelles. Dynein light chain (LC) subunits are conserved across species; however, the molecular contribution of LCs to dynein function remains controversial. One model suggests that LCs act as cargo-binding scaffolds. Alternatively, LCs are proposed to stabilize the intermediate chains (ICs) of the dynein complex. To examine the role of LCs in dynein function, we used Saccharomyces cerevisiae, in which the sole function of dynein is to position the spindle during mitosis. We report that the LC8 homologue, Dyn2, localizes with the dynein complex at microtubule ends and interacts directly with the yeast IC, Pac11. We identify two Dyn2-binding sites in Pac11 that exert differential effects on Dyn2-binding and dynein function. Mutations disrupting Dyn2 elicit a partial loss-of-dynein phenotype and impair the recruitment of the dynein activator complex, dynactin. Together these results indicate that the dynein-based function of Dyn2 is via its interaction with the dynein IC and that this interaction is important for the interaction of dynein and dynactin. In addition, these data provide the first direct evidence that LC occupancy in the dynein motor complex is important for function.

scholarly journals Direct evidence for a key role of protein kinase C in the development of vasospasm after subarachnoid hemorrhage

1992 ◽  
Vol 76 (4) ◽  
pp. 635-639 ◽  
Author(s):  
Shigeru Nishizawa ◽  
Nobukazu Nezu ◽  
Kenichi Uemura
Keyword(s):  
Signal Transduction ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Direct Evidence ◽  
Control Group ◽  
Content Type ◽  
Kinase C ◽  
Protein Kinase C Activity ◽  
Fine Print

✓ Vascular contraction is induced by the activation of intracellular contractile proteins mediated through signal transduction from the outside to the inside of cells. Protein kinase C plays a crucial role in this signal transduction. It is hypothesized that protein kinase C plays a causative part in the development of vasospasm after subarachnoid hemorrhage (SAH). To verify this directly, the authors measured protein kinase C activity in canine basilar arteries in an SAH model with (γ-32P)adenosine triphosphate and the data were compared to those in a control group. Protein kinase C is translocated to the membrane from the cytosol when it is activated, and the translocation is an index of the activation; thus, protein kinase C activity was measured both in the cytosol and in the membrane fractions. Protein kinase C activity in the membrane in the SAH model was remarkably enhanced compared to that in the control group. The percentage of membrane activity to the total was also significantly greater in the SAH vessels than in the control group, and the percentage of cytosol activity in the SAH group was decreased compared to that in the control arteries. The results indicate that protein kinase C in the vascular smooth muscle was translocated to the membrane from the cytosol and was activated when SAH occurred. It is concluded that this is direct evidence for a key role of protein kinase C in the development of vasospasm.

The Peopling of the Baraba Forest-Steppe in the Neolithic: Cranial Evidence

2021 ◽  
Vol 49 (1) ◽  
pp. 133-146
Author(s):  
T. A. Chikisheva ◽  
D. V. Pozdnyakov
Keyword(s):  
Local Population ◽  
Principal Component ◽  
Early Holocene ◽  
Russian Plain ◽  
Northern Eurasia ◽  
Forest Steppe ◽  
Early Neolithic ◽  
Population Continuity ◽  
Lake Systems

On the basis of statistical analysis of craniometric data relating to Mesolithic and Neolithic samples from northern Eurasia, we discuss the peopling of the Baraba forest-steppe in the Early Holocene. This region is represented by samples from Sopka-2/1 (early sixth millennium BC), Protoka (late fifth to early fourth millennia BC), Korchugan (early-mid sixth millennium BC), and Vengerovo-2A (late sixth millennium BC). The results of the principal component analysis are interpreted in the context of debates over the role of autochthonous traditions in the Neolithic. During the Preboreal period (10 ka BP), large parts of the Baraba forest-steppe were flooded by the transgression of lake systems during climatic warming. This may have caused depopulation, lasting for at least a millennium. The Early Holocene people of Baraba were an offshoot of Meso-Neolithic populations of the northwestern Russian Plain. On that basis, the Early Neolithic populations of Baraba were formed. Direct population continuity is traceable only through the Chalcolithic. Since the late sixth millennium BC, however, the local population had incorporated migrants from the Pit-Comb Ware area in the central Russian Plain and, indirectly (via the Neolithic Altai), from the Cis-Baikal area.

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