Influence of liver fat on post-partum hormone profiles in dairy cows

1987 ◽  
Vol 45 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Elaine D. Watson ◽  
Linda A. Williams
Keyword(s):  
Dairy Cows ◽  
Jugular Vein ◽  
Peak Plasma ◽  
Post Partum ◽  
Fatty Infiltration ◽  
Plasma Concentrations ◽  
Liver Fat ◽  
Plasma Progesterone ◽  
Liver Biopsies ◽  
The Difference

AbstractCows (no. = 11) were classified from liver biopsies taken 7 to 10 days after calving as having mild (FL1, no. = 6), or moderate (FL2, no. = 5) fatty infiltration of the liver. Blood samples were collected from the jugular vein: (1) three times weekly and assayed for progesterone and oestradiol; (2) at 10-min intervals for 8-h periods on three occasions during the early post-partum period and assayed for LH; (3) every 2 h, with ovarian palpation performed every 4 h around the second or third post-partum ovulation, and assayed for LH and oestradiol.Mean weekly concentrations of oestradiol were not significantly different between FL1 and FL2 cows. FL2 cows tended to have short (< 17 days) first cycles (3/4 FL2 v. 0/5 FL1) with significantly lower concentrations of plasma progesterone (P < 0·05). Mean basal concentrations of LH in the samples collected at 10-min intervals tended to be lower in FL2 than in FL1 cows; however, the difference was significant only in the 2nd week of sampling (P = 0·05). Mean basal concentrations of LH were significantly higher in weeks 3 and 4 than in week 2 in FL1 but not in FL2 cows (P < 0·05). The frequency and amplitude of LH pulses were not affected by week of sampling or by fat content of the liver. The total area under the profile of LH during the pre-ovulatory surge was lower in FL2 than in FL1 cows (P < 0·05) but peak plasma concentrations of pre-ovulatory oestradiol and timing of ovulation after the LH surge were similar.

Relationship between plasma leptin concentration and reproductive function in dairy cows

2002 ◽  
Vol 2002 ◽  
pp. 2-2 ◽  
Author(s):  
GE Mann ◽  
D Blache
Keyword(s):  
Dairy Cows ◽  
Post Partum ◽  
Plasma Concentrations ◽  
Reproductive Function ◽  
Progesterone Concentration ◽  
Metabolic Demand ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
High Incidence ◽  
Plasma Leptin

In modern dairy cows, poor fertility is an ever-increasing problem. Milk progesterone analysis has revealed that this poor fertility is associated with a high incidence of reproductive cycle problems during the post partum period and with reduced progesterone secretion following mating. One of the likely causes of these problems is the increasing metabolic demand placed on these cows by increasing milk yield. In recent years, the search for an endocrine link between nutritional status and reproductive function has focussed on leptin. The aim of the present study was to determine whether plasma concentrations of leptin differ between cows with and without post partum reproductive problems and to determine if any relationship exists between plasma leptin and post mating plasma progesterone concentration.

scholarly journals Relationship between plasma concentrations of estradiol-17β and progesterone and the distribution of epithelial and inflammatory cells in the external opening of the river buffaloes’ uterine cervix during the gestational period

2021 ◽  
Vol 24 (3) ◽  
pp. 365-375
Author(s):  
M. Goli ◽  
E. Ayen ◽  
S. Hassanzadeh ◽  
M. H. Khadem Anssari
Keyword(s):  
Uterine Cervix ◽  
Jugular Vein ◽  
Plasma Concentrations ◽  
Inflammatory Cells ◽  
Gestation Period ◽  
Blood Levels ◽  
External Opening ◽  
Microscopic Evaluation ◽  
The Difference ◽  
Estradiol 17Β

Changes in the distribution of the epithelial and inflammatory cells in the external opening of the uterine cervix in river buffaloes at three different occasions during the gestation period were investigated by light microscopic evaluation of mucus smears prepared by wet swab sampling and Giemsa-stained. Forty five pregnant river buffaloes that were in the first (n=15), second (n=15) and third (n=15) approximately equal parts of their gestation period were sampled twice concurrently by individual wet swabs from the external opening of the uterine cervix. Five smears were prepared from each sample. Immediately after mucus sampling, blood samples were obtained from the jugular vein of each animal, centrifuged immediately and stored at -20 oC to assay the plasma levels of estradiol-17β and progesterone. The results showed that changes in the percentage of the vacuolated and non-vacuolated epithelial cells, lymphocytes, eosinophils, and basophils were not statistically significant between the groups, which suggests that as the pregnancy progressed, changes in the blood levels of estradiol-17β and progesterone had little or no effect on the distribution of these cells in the region, but changes in the percentage of neutrophils were statistically significant between the groups. It was also found that changes in the percentage of macrophages between the second and third trimesters of the gestation period were not significant, but the difference between the first and the other trimesters was statistically significant. It was concluded that the percentages of neutrophils and macrophages increase significantly as the plasma concentrations of progesterone decrease during the gestation.

scholarly journals Comparative changes in plasma concentrations of progesterone, oestradiol and LH during the ovulatory cycle in a multiple ovulating male line and a single ovulating traditional line of turkeys

Reproduction ◽  
2002 ◽  
pp. 127-133 ◽  
Author(s):  
S Buchanan ◽  
GW Robertson ◽  
PM Hocking
Keyword(s):  
Peak Plasma ◽  
Ovarian Tissue ◽  
Plasma Concentrations ◽  
High Growth ◽  
Correlated Response ◽  
Ovulatory Cycle ◽  
Plasma Progesterone ◽  
Multiple Ovulation ◽  
Male Line ◽  
The Mean

The aim of this study was to compare the profile of circulating concentrations of LH, progesterone and oestradiol in a multiple ovulating male line with that of a single ovulating line of traditional turkeys. Plasma samples from seven traditional and 12 male-line turkeys were obtained every 3 h for 36 h. Male-line and traditional turkeys had single peaks of LH and progesterone that were of similar duration in both lines. The mean height of the plasma peaks of LH and progesterone were similar in the two lines and there was no detectable peak plasma oestrogen concentration. Mean plasma concentrations of LH and oestrogen were higher in single compared with multiple ovulating turkeys, whereas there were no differences in mean plasma progesterone concentrations. The results indicate that the multiple ovulation state in genetically selected high-growth lines of turkey may be the result of a correlated response in the steroidogenic capacity of ovarian tissue associated with low plasma concentrations of oestrogen rather than of a disturbance in the hormone profile of the ovulatory cycle.

Reproductive function in average and high yielding dairy cows

2001 ◽  
Vol 26 (2) ◽  
pp. 495-498 ◽  
Author(s):  
Victoria J. Taylor ◽  
Andrew J. Hattan ◽  
Emma C.L. Bleach ◽  
David E. Beever ◽  
D. Claire Wathes
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Post Partum ◽  
Reproductive Function ◽  
Reproductive Activity ◽  
Corpora Lutea ◽  
Genetic Merit ◽  
Standard Management ◽  
Service Conception ◽  
The Difference

AbstractThe resumption of reproductive activity in the early post partum period and the subsequent fertility of dairy cows are of considerable practical and economic importance to the producer. Regular milk progesterone analysis was used in this study to monitor the re-establishment of reproductive cyclicity in animals of average (AGM, n=20) and high genetic merit (HGM, n=28) kept under standard management conditions. The profiles obtained were then characterised into normal and four different types of abnormal activity. Resumption of cycles occurred significantly later in the higher yielding cows than in those of average genetic merit (23 ±2.3 days in HGM cows and 15 ± 1.6 days in AGM cows; P=0.0089). There was a significant increase in the amount of abnormal cycles encountered in the high genetic merit animals compared with average yielding animals (61% and 30% respectively; χ2 test, P=0.045); in particular, the delayed occurrence of ovulation post partum (11% vs 0%) and an increased incidence of persistent corpora lutea (32% vs 20%). For high genetic merit animals, the incidence of abnormal cycles was associated with increased peak milkyields (abnormal cycles: peak milk yield 54.7 ± 1.11 kg/day; normal cycles: peak milk yield 46.4 ± 1.35 kg/day, P=0.002). This relationship was not apparent in average genetic merit animals. First service conception rates were 50% (9/18) for AGM cows and 38% (10/26) for HGM cows (χ2 test, n.s.). The calving to conception interval was 98 ± 9.7 days for AGM animals (n=l7) and 105 ± 9.1 days for HGMs (n=24) (t-test, n.s.). In conclusion, this study confirms that high genetic merit dairy cows have compromised reproductive function in terms of a delayed resumption of cyclicity and an increased incidence of abnormal progesterone cycles, which was associated with higher peak milk yields. There was also a reduced first service conception rate in high yielders although the difference was not significant with this group size.

Exercise-dependent growth hormone release is linked to markers of heightened central adrenergic outflow

2000 ◽  
Vol 89 (2) ◽  
pp. 629-635 ◽  
Author(s):  
Arthur Weltman ◽  
Cathy J. Pritzlaff ◽  
Laurie Wideman ◽  
Judy Y. Weltman ◽  
Jeffery L. Blumer ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Regression Analysis ◽  
Exercise Intensity ◽  
Acute Exercise ◽  
Peak Plasma ◽  
Linear Regression Analysis ◽  
Plasma Concentrations ◽  
Intensity Change ◽  
Plasma Norepinephrine ◽  
The Difference

To test the hypothesis that heightened sympathetic outflow precedes and predicts the magnitude of the growth hormone (GH) response to acute exercise (Ex), we studied 10 men [age 26.1 ± 1.7 (SE) yr] six times in randomly assigned order (control and 5 Ex intensities). During exercise, subjects exercised for 30 min (0900–0930) on each occasion at a single intensity: 25 and 75% of the difference between lactate threshold (LT) and rest (0.25LT, 0.75LT), at LT, and at 25 and 75% of the difference between LT and peak (1.25LT, 1.75LT). Mean values for peak plasma epinephrine (Epi), plasma norepinephrine (NE), and serum GH concentrations were determined [Epi: 328 ± 93 (SE), 513 ± 76, 584 ± 109, 660 ± 72, and 2,614 ± 579 pmol/l; NE: 2.3 ± 0.2, 3.9 ± 0.4, 6.9 ± 1.0, 10.7 ± 1.6, and 23.9 ± 3.9 nmol/l; GH: 3.6 ± 1.5, 6.6 ± 2.0, 7.0 ± 2.0, 10.7 ± 2.4, and 13.7 ± 2.2 μg/l for 0.25, 0.75, 1.0, 1.25, and 1.75LT, respectively]. In all instances, the time of peak plasma Epi and NE preceded peak GH release. Plasma concentrations of Epi and NE always peaked at 20 min after the onset of Ex, whereas times to peak for GH were 54 ± 6 (SE), 44 ± 5, 38 ± 4, 38 ± 4, and 37 ± 2 min after the onset of Ex for 0.25–1.75LT, respectively. ANOVA revealed that intensity of exercise did not affect the foregoing time delay between peak NE or Epi and peak GH (range 17–24 min), with the exception of 0.25LT ( P < 0.05). Within-subject linear regression analysis disclosed that, with increasing exercise intensity, change in (Δ) GH was proportionate to both ΔNE ( P = 0.002) and ΔEpi ( P = 0.014). Furthermore, within-subject multiple-regression analysis indicated that the significant GH increment associated with an antecedent rise in NE ( P = 0.02) could not be explained by changes in Epi alone ( P = 0.77). Our results suggest that exercise intensity and GH release in the human may be coupled mechanistically by central adrenergic activation.

Blood clinicopathological differences between type I and II ketosis in dairy cows

2015 ◽  
Author(s):  
XU Chuang ◽  
Shen Tai-yu ◽  
YAO Yuan ◽  
Yu Hong-jiang ◽  
XIA Cheng ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Dairy Cow ◽  
Plasma Concentrations ◽  
Elisa Test ◽  
Type I ◽  
Type Ii ◽  
Hydroxybutyric Acid ◽  
Cattle Farm ◽  
The Difference ◽  
Heilongjiang Of China

The purpose was to determine the difference of blood clinicopathological changes between type I and type II ketosis in dairy cow. Fifty-eight cows, from dairy cattle farm in Heilongjiang of China, were included. An ELISA test was used to evaluate the blood indicators. The plasma concentrations of beta-hydroxybutyric acid (BHBA) and insulin sensitivity decreased, and the plasma concentration of glucose (Glc), non-esterified fatty acid (NEFA) and bilirubin content increased in type II ketosis group compared with the type I ketosis group. These results showed that there was a difference in etiology between type II ketosis and type I ketosis. Type II ketosis was not only associated with energy metabolism and insulin resistance, but also with oxidative stress and liver function. It laid the foundation for further investigate the mechanism and prevention of type II ketosis in the future.

Studies on plasma progesterone concentrations and fertility in Friesland dairy cows during the post-partum period

1981 ◽  
Vol 97 (3) ◽  
pp. 649-654 ◽  
Author(s):  
D. H. Holness ◽  
G. W. Sprowson ◽  
Carole Sheward ◽  
Amanda Geel
Keyword(s):  
Dairy Cows ◽  
Early Pregnancy ◽  
Post Partum ◽  
Ovarian Activity ◽  
Plasma Progesterone ◽  
Low Concentrations ◽  
The Mean ◽  
Post Partum Period ◽  
Embryo Loss

SUMMARYPlasma progesterone concentrations were monitored in 69 lactating Friesland dairy cows during the post-partum period. Cyclic ovarian activity resumed in most cows within 40 days of calving. Forty-eight per cent of cows remained pregnant to their first insemination, 41% returned to oestrus before day 30 after insemination, and a further 11% returned to oestrus between days 30 and 88 after insemination.The mean of progesterone values on days 11 to 4 before insemination tended to be higher in cows that conceived than in cows that did not conceive. These differences were signficant on day –8 (P <0·05) and on days –8, –7, –6 and –5 combined (P <0·01). Cows in which the concentration of progesterone in plasma did not rise above 4·5 ng/ml during this period did not conceive. After insemination, mean progesterone concentrations between pregnant and non-pregnant cows diverged after day +8. Relatively low concentrations of plasma progesterone (< 1 ng/ml) up to day +8 after insemination did not preclude conception. There was no indication that embryo loss between days +30 and +88 after insemination was related to an insufficiency of ovarian progesterone during early pregnancy.

scholarly journals Relationship between metabolic hormones and ovulation of dominant follicle during the first follicular wave post-partum in high-producing dairy cows

Reproduction ◽  
2007 ◽  
Vol 133 (1) ◽  
pp. 155-163 ◽  
Author(s):  
Chiho Kawashima ◽  
Saori Fukihara ◽  
Mayumi Maeda ◽  
Etsushi Kaneko ◽  
Carlos Amaya Montoya ◽  
...  
Keyword(s):  
Blood Flow ◽  
Dairy Cows ◽  
Ovarian Function ◽  
Post Partum ◽  
Plasma Concentrations ◽  
Reproductive Hormones ◽  
Follicular Growth ◽  
Dominant Follicle ◽  
Follicular Wave ◽  
Igf I

Recent studies suggest that IGF-I is a crucial regulatory factor in follicular growth during earlypost-partumperiod. The aim of the present study was to determine in detail the changing profiles of metabolic and reproductive hormones in relation to ovulation of the dominant follicle (DF) of the first follicular wavepost-partumin high-producing dairy cows. Plasma concentrations of related hormones in 22 multiparous Holstein cows were measured from 4 weeks pre-partum to 3 weekspost-partum, and the development of DF was observed with colour Doppler ultrasound. Thirteen cows showed ovulation by 15.2 dayspost-partum. Anovulatory cows showed higher GH and lower IGF-I levels than those in ovulatory cows during the peri-partum period. Each DF developed similarly, and a clear blood flow in the follicle wall was observed despite ovulation or anovulation. In addition, detailed endocrine profiles were analyzed in 9 out of the 22 cows. Five cows showed an increase in plasma oestradiol-17β (E2) with follicular growth followed by E2 peak, LH surge and ovulation. In these cows, plasma IGF-I concentrations remained high until 10 dayspost-partumfollowed by a gradual decrease. Subsequently, the insulin level increased together with the E2 peak towards ovulation. These profiles were not observed in anovulatory cows. In conclusion, our data strongly support the concept that IGF-I and insulin represent ‘metabolic signals’ of the resumption of ovarian functionpost-partumin high-producing dairy cows. Moreover, we provide the first visual evidence that both ovulatory and anovulatory DFs of the first follicular wavepost-partumare similarly supplied with active blood flow.

Attenuation of preovulatory gonadotrophin surges by epostane: a new inhibitor of 3β-hydroxysteroid dehydrogenase

1988 ◽  
Vol 118 (1) ◽  
pp. 59-68 ◽  
Author(s):  
L. V. DePaolo
Keyword(s):  
Peak Plasma ◽  
Plasma Concentrations ◽  
Hydroxysteroid Dehydrogenase ◽  
Inhibitory Effects ◽  
Plasma Progesterone ◽  
Ru 486 ◽  
Progesterone Secretion ◽  
Progesterone Antagonist

ABSTRACT Epostane, an inhibitor of 3β-hydroxysteroid dehydrogenase, was administered orally to pro-oestrous rats to evaluate further a possible role for preovulatory progesterone secretion in eliciting surges of LH and FSH. Whereas a dose of 10 mg epostane/kg had essentially no effects on preovulatory gonadotrophin surges and ovulation, 200 mg epostane/kg markedly attenuated LH and FSH surges and blocked ovulation. A dose of 50 mg epostane/kg exerted effects on LH and FSH surges and ovulation intermediate between those of doses of 10 and 200 mg/kg. Plasma concentrations of progesterone were significantly lower in all anovulatory epostane-treated rats at 18.00 and 22.00 h on pro-oestrus than those measured in vehicle-treated rats. Concurrent injection of 2 mg progesterone in rats given 200 mg epostane/kg restored gonadotrophin surges to normal, but consistently failed to reverse the inhibitory effects of epostane on ovulation. Peak plasma progesterone levels produced by the progesterone injections were eight- to tenfold higher than the highest levels measured in vehicle-treated rats during the afternoon of pro-oestrus. Insertion of progesterone capsules was less effective than injections of progesterone in restoring gonadotrophin surges to normal, even though peak plasma progesterone concentrations achieved after insertion of two 20 mm long progesterone capsules were double the peak progesterone concentrations measured in control rats. Nevertheless, taken together with recent reports showing attenuation of preovulatory gonadotrophin surges by the progesterone antagonist RU 486 (17β-hydroxy-11β-[4-dimethyl-aminophenyl]-17α-[prop-1-ynl]estra-4,9-diene-3-one), the present results provide support for a role of preovulatory progesterone secretion in enhancing oestrogen-dependent LH/FSH surges on pro-oestrus. J. Endocr. (1988) 118, 59–68

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