Variegate porphyria induces plasma and neutrophil oxidative stress: effects of dietary supplementation with vitamins E and C

Our aim was to analyse the influence of variegate porphyria (VP) on the antioxidant defenses and markers of oxidative damage and inflammation in plasma and neutrophils and the effects of dietary supplementation with vitamins E and C on these parameters in plasma, neutrophils and erythrocytes. Twelve women affected by VP and twelve pair-matched healthy control women participated in a double-blind crossover study. Each participant took 50 mg/d of vitamin E and 150 mg/d of vitamin C, or a placebo, for 6 months, by consuming an almond-based beverage as the vehicle. Women affected by VP presented higher C-reactive protein and malondialdehyde (MDA) circulating levels. Plasma antioxidant defenses were not different between porphyric and control women. Neutrophils from VP women presented decreased catalase (CAT) and glutathione reductase (GR) activities together with increased protein carbonyl levels. Reactive oxygen species (ROS) production from stimulated neutrophils was also higher in porphyric women than their controls. Dietary supplementation was effective in increasing α-tocopherol levels in neutrophils and in reducing MDA levels in plasma. Erythrocyte CAT and GR activities were enhanced by the enriched beverage only in the control subjects. In conclusion, women affected by VP present a situation of inflammation, plasma oxidative damage and neutrophils more primed to the oxidative burst, with decreased antioxidant activities and increased ROS production capabilities and protein oxidative damage. Dietary supplementation with vitamin E (50 mg/d) and vitamin C (150 mg/d) for 6 months decreased plasma oxidative damage and enhanced the erythrocyte activities of CAT and GR.

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Investigating the effects of vitamins E and C on oxidative stress and hematological parameters among power plant workers: A double-blind randomized controlled clinical trial

Toxicology and Industrial Health ◽

10.1177/0748233720908993 ◽

2020 ◽

Vol 36 (2) ◽

pp. 99-109

Author(s):

Majid Bagheri Hosseinabadi ◽

Narges Khanjani ◽

Pirasteh Norouzi ◽

Mehdi Mirzaii ◽

Jamal Biganeh ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Power Plant ◽

Vitamin C ◽

Hematological Parameters ◽

Control Group ◽

Double Blind ◽

Randomized Controlled ◽

Vitamins E And C ◽

The Mean

The present study aimed to determine the effect of taking antioxidant vitamins including vitamins E and C in reducing oxidative stress levels and improving blood parameters. This double-blind randomized controlled trial study was conducted among the employees working in different parts of a power plant in Semnan, Iran, in 2017. A total of 91 employees were randomly allocated to four groups including vitamin E (400 units per day), vitamin C (1000 mg per day), vitamin E + C for 90 days, and control. The levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (Cat), and total antioxidant capacity (TAC) in plasma, and hematological parameters were measured in the participants before and after the intervention. A significant increase was seen in the mean level of SOD, Cat, and TAC in the vitamin-treated groups as well as a significant decrease in mean MOD in vitamin C and vitamin E groups after the intervention. In the intervention groups, the number of red blood cells, hematocrit, and the level of mean corpuscular hemoglobin (MCH) and MCH concentration significantly increased. After the intervention, the mean levels of MDA, SOD, and Cat in vitamin E group were significantly lower than the control group. The mean level of TAC decreased only in the vitamin C group compared to the control group. Taking vitamins E and C as nonenzymatic scavengers of free radicals appears to decrease lipid peroxidation and increase the level of antioxidant enzymes, which can be imbalanced by exposure to extremely low-frequency electromagnetic fields in power plant employees. Furthermore, some hematological parameters can be improved by consuming these vitamins.

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Feeding antioxidant vitamin and vegetable oils to broilers: vitamin E reduced negative effect of soybean oil on immune response and meat lipid oxidation

Animal Production Science ◽

10.1071/an16677 ◽

2018 ◽

Vol 58 (10) ◽

pp. 1829

Author(s):

M. Mohiti-Asli ◽

M. Ghanaatparast-Rashti

Keyword(s):

Immune Response ◽

Vitamin E ◽

Vitamin C ◽

Soybean Oil ◽

Lipid Oxidation ◽

Vegetable Oil ◽

Canola Oil ◽

Cellular Response ◽

Dietary Supplementation ◽

Antioxidant Vitamin

This study investigated the effect of feeding vitamin E, vitamin C, and two sources of vegetable oil on immune response and meat quality of broilers. A total of 320 one-day-old chicks were used in a completely randomised design with eight treatments arranged as a 2 × 2 × 2 factorial with two levels of vitamin E (0 and 200 mg/kg), two levels of vitamin C (0 and 1000 mg/kg), and two sources of vegetable oil (soybean and canola). Dietary supplementation of either vitamin E or C increased (P < 0.05) secondary humoral response, whereas oil sources had no significant effect. Broilers fed soybean oil had lower cellular response to the phytohemagglutinin skin test than those fed canola oil in diet, and supplementation of vitamin E increased cellular immune response. However, fat, cholesterol and pH of meat were not affected by source of oil or antioxidants, lipid oxidation was higher (P < 0.05) in thigh and breast meat of broilers fed soybean oil than canola oil. Dietary supplementation of vitamin E decreased (P < 0.05) lipid oxidation in thigh and breast of broilers fed diet containing soybean oil, without any effect on meat oxidation of those fed canola oil. Dietary supplementation of vitamin C increased lipid oxidation in thigh meat of broilers (P < 0.05). It can be concluded that inclusion of soybean oil to the diet, compared with canola oil, increased need for antioxidant. Vitamin E had beneficial effects on immune response and reduced meat lipid oxidation; nonetheless future studies should explore the antioxidant effect of vitamin C in stored meat.

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In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats

Archives of Toxicology ◽

10.1007/s002040100219 ◽

2001 ◽

Vol 75 (2) ◽

pp. 88-96 ◽

Cited By ~ 183

Author(s):

Fatih Gultekin ◽

Namik Delibas ◽

Sulhattin Yasar ◽

Ibrahim Kilinc

Keyword(s):

Vitamin E ◽

Vitamin C ◽

Oxidative Damage ◽

Protective Role ◽

Antioxidant Systems ◽

Chlorpyrifos Ethyl

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Impact of Vitamins C and E Dietary Supplementation on Leukocyte Profile of Layer Hens Exposed to High Ambient Temperature and Humidity

Acta Veterinaria Brno ◽

10.2754/avb201079030377 ◽

2010 ◽

Vol 79 (3) ◽

pp. 377-383 ◽

Cited By ~ 5

Author(s):

Joachim Joseph Ajakaiye ◽

Alcides Perez-Bello ◽

Angel Mollineda-Trujillo

Keyword(s):

Vitamin E ◽

Vitamin C ◽

Ambient Temperature ◽

Harmful Effect ◽

Dietary Supplementation ◽

High Ambient Temperature ◽

Control Group ◽

Physiological Status ◽

Temperature And Humidity ◽

Layer Hens

A 30-day experiment involving 720 White Leghorn layer hens was carried out to evaluate the effects of vitamins C and E on leukocyte and heterophil/lymphocytes ratios of the layer hens during the natural summer conditions. The birds were divided into 4 groups, one control with basal diet and three experimental with diet supplemented with vitamin C, vitamin E, or combination of both vitamins. Each group was divided into 4 subgroups. Exposure of layer hens to 31 ± 3 °C and 33 ± 0 °C ambient temperature and 84.6% and 81.5% relative humidity during the experiment caused an increase in the value of temperature humidity index of 15.5 above the threshold value of 70 established for this species. Total white blood cells, lymphocytes, and eosinophil levels were significantly (p < 0.001) higher in all treated groups compared to control. Similarly, heterophil/lymphocyte ratios of 0.51, 0.52, and 0.50 for groups with diet supplemented with vitamin C, vitamin E and vitamins C + E, respectively, were significantly (p < 0.001) lower compared to 0.59 recorded in the control group. However, heterophil, basophil and monocyte values were significantly (p < 0.001) higher in the control group compared to all treated groups. The results of the present study showed that dietary supplementation of vitamins C and E, or their combination, reduces the harmful effect of high ambient temperature and humidity on the leukocyte indicators of exposed layer hens, thereby alleviating the stress imposed on their thermoregulatory mechanism and physiological status.

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Effects of dietary supplementation with vitamin C or vitamin E on cardiac lipid peroxidation and growth performance in broilers at risk of developing ascites syndrome

American Journal of Veterinary Research ◽

10.2460/ajvr.2002.63.673 ◽

2002 ◽

Vol 63 (5) ◽

pp. 673-676 ◽

Cited By ~ 19

Author(s):

Gonzalo Villar-Patino ◽

Antonio Diaz-Cruz ◽

Ernesto Avila-Gonzalez ◽

Raquel Guinzberg ◽

Jose L. Pablos ◽

...

Keyword(s):

At Risk ◽

Lipid Peroxidation ◽

Vitamin E ◽

Vitamin C ◽

Growth Performance ◽

Dietary Supplementation ◽

Cardiac Lipid ◽

Ascites Syndrome

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Vitamin E, Vitamin C, or Losartan Is Not Nephroprotectant against Cisplatin-Induced Nephrotoxicity in Presence of Estrogen in Ovariectomized Rat Model

International Journal of Nephrology ◽

10.1155/2012/284896 ◽

2012 ◽

Vol 2012 ◽

pp. 1-10 ◽

Cited By ~ 19

Author(s):

Mehdi Nematbakhsh ◽

Zahra Pezeshki ◽

Fatemeh Eshraghi-Jazi ◽

Farzaneh Ashrafi ◽

Hamid Nasri ◽

...

Keyword(s):

Vitamin E ◽

Vitamin C ◽

Kidney Tissue ◽

Serum Levels ◽

Ovariectomized Rats ◽

Estradiol Valerate ◽

Group 4 ◽

Vitamins E And C ◽

Losartan Group ◽

Group 2

Background. The nephroprotective effect of vitamins E and C or losartan against cisplatin (CP)- induced nephrotoxicity when they are accompanied by estrogen was investigated.Methods. The ovariectomized rats received estradiol valerate for two weeks. At the end of the first week, a single dose of CP (7 mg/kg, IP) was also administered, and they received placebo (group 1), vitamin E (group 2), vitamin C (group 3), or losartan (group 4) every day during the second week, and they were compared with another three control groups.Results. CP alone increased the serum levels of blood urea nitrogen (BUN), creatinine (Cr), and kidney tissue damage score (KTDS), significantly (P<0.05), however at the presence of estradiol and CP, vitamin C, vitamin E, or losartan not only did not decrease these parameters, but also increased them significantly (P<0.05). The serum level of superoxidase dismutase (SOD) was reduced by CP (P<0.05), but it was increased when estradiol or estradiol plus vitamin C or losartan were added (P<0.05).Conclusion. The particular pharmacological dose of estrogen used in this study abolish the nephroprotective effects vitamins C and E or losartan against CP-induced nephrotoxicity.

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Cardiotoxicity in rats induced by methidathion and ameliorating effect of vitamins E and C

Human & Experimental Toxicology ◽

10.1191/0960327104ht456oa ◽

2004 ◽

Vol 23 (7) ◽

pp. 323-329 ◽

Cited By ~ 26

Author(s):

Turhan Yavuz ◽

Irfan Altuntas ◽

Namik Delibas ◽

Bekir Yildirim ◽

Ozden Candir ◽

...

Keyword(s):

Vitamin E ◽

Vitamin C ◽

Troponin I ◽

Corn Oil ◽

Histopathological Examination ◽

Heart Tissue ◽

Control Group ◽

Heart Damage ◽

Vitamins E And C ◽

Diffuse Loss

We have examined the effect of subchronic methidathion (MD) administration on heart damage, and have evaluated possible ameliorating effects of a combination of vitamins E and C against MD toxicity. The experimental groups were: control group, rats treated with 5 mg/kg MD and rats treated with 5 mg/kg body weight MD plus vitamin E and vitamin C (MD+Vit). The groups were given MD by gavage 5 days a week for four weeks at a dose level of 5 mg/kg/day (MD and MD+Vit) by using corn oil as the vehicle. Vitamin E and vitamin C were injected at doses of 50 mg/kg i.m. and 20 mg/kg i.p., respectively, after the treatment with MD in the MD+Vit group. The levels of malondialdehyde (MDA) were determined in the heart tissue, and the levels of cardiac troponin I (TnI) in serum. An autoanalyser was used to determine the serum activities of cholinesterase (ChE). Histopathological examination was carried out in the heart tissue. MDA significantly increased in the MD group as compared to controls (P <0.01). When MD was given concurrently with vitamins E and C, the increase in MDA was significantly less (P <0.01). ChE activity significantly decreased in the MD group as compared to controls (P <-0.01). When MD was given concurrently with vitamins E and C, the decrease in ChE activity was significantly higher (P <-0.05). The serum TnI levels significantly increased in the MD group as compared to controls (P <-0.01). When MD was given concurrently with vitamins E and C, the increase in the serum TnI was significantly less (P <-0.01). MD caused the diffuse loss of striation and myocytolysis of the cardiomyocytes, whereas the combination of vitamins E and C caused a significant decrease in these effects of MD. In conclusion, subchronic MD administration caused heart damage and, in addition, treatment with a combination of vitamins E and C after the administration of MD reduced heart damage caused by MD.

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