Abnormalities in growth, development and physiological responses to biotic and abiotic stress in potato (Solanum tuberosum) transformed with Arabidopsis ETR1

2003 ◽  
Vol 141 (3-4) ◽  
pp. 333-347 ◽  
Author(s):  
M. M. HAINES ◽  
P. J. SHIEL ◽  
J. K. FELLMAN ◽  
P. H. BERGER
Keyword(s):  
Solanum Tuberosum ◽  
Receptor Gene ◽  
Plant Morphology ◽  
Transgenic Potato ◽  
Potato Industry ◽  
Primary Concern ◽  
Biotic And Abiotic Stress ◽  
Tuber Dormancy ◽  
Ethylene Action ◽  
Pleiotrophic Effects

Sprouted potato tubers lose nutritional value, and their increased content of reducing sugar renders them unsuitable for the french fry and chipping industries. Thus, tuber sprouting in storage is a primary concern of the potato industry, and is currently controlled by application of chemical sprout suppressants. Such suppressants are subject to widespread regulation by governmental agencies. The present study was initiated to investigate the role of the plant hormone ethylene in potato (Solanum tuberosum L.) tuber dormancy release. To this end, potato cv. ‘Russet Burbank’ was transformed with ETR1, an ethylene receptor gene from Arabidopsis. The resulting clones were genetically characterized to verify integration and expression of the ETR1 mRNA and protein. Transgenic clones showed developmental and morphologic changes in plant and tuber, and differing responses to environmental stress and pathogen attack relative to untransformed controls. Lengthening of dormancy was also observed in ETR1 antisense clones stored at 4 °C. Transgenic potato clones treated with solutions of silver thiosulfate (STS), an ethylene action inhibitor, showed restoration of normal plant morphology, while controls were unaffected. These results demonstrate the pleiotrophic effects of ethylene in potato, and identify a need for additional studies on the relationship between ethylene and tuber dormancy.

Localization of Ds-transposon containing T-DNA inserts in the diploid transgenic potato: linkage to the R1 resistance gene against Phytophthora infestans (Mont.) de Bary

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 249-257 ◽  
Author(s):  
A. El-Kharbotly ◽  
J. M. E. Jacobs ◽  
B. te Lintel Hekkert ◽  
W. J. Stiekema ◽  
A. Pereira ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Phytophthora Infestans ◽  
Resistance Gene ◽  
Dna Sequences ◽  
Transgenic Potato ◽  
Potato Genome ◽  
Rflp Map ◽  
Polymerase Chain ◽  
R1 Gene ◽  
Map Location

The Dissociation transposable element (Ds) of maize containing NPTII was introduced into the diploid potato (Solanum tuberosum) clone J91-6400-A16 through Agrobacterium tumefaciens mediated transformation. Genomic DNA sequences flanking the T-DNAs from 312 transformants were obtained with inverse polymerase chain reaction or plasmid rescue techniques and used as probes for RFLP linkage analysis. The RFLP map location of 60 T-DNAs carrying Ds–NPTII was determined. The T-DNA distribution per chromosome and the relative distance between them appeared to be random. All 12 chromosomes have been covered with Ds-containing T-DNAs, potentially enabling tagging of any gene in the potato genome. The T-DNA insertions of two transformants, BET92-Ds-A16-259 and BET92-Ds-A16-416, were linked in repulsion to the position of the resistance gene R1 against Phytophthora infestans. After crossing BET92-Ds-A16-416 with a susceptible parent, 4 desired recombinants (Ds carrying T-DNA linked in coupling phase with the R1 gene) were discovered. These will be used for tagging the R1 gene. The efficiency of the pathway from the introduction to localization of T-DNAs is discussed. Key words : Solanum tuberosum, Phytophthora infestans, Ds element, transposon tagging, R genes, euchromatin.

scholarly journals Challenges of Seed Potato (Solanum tuberosum L.) Production and Supply System in Bangladesh - A Review

2016 ◽  
Vol 13 (1) ◽  
pp. 173-188
Author(s):  
Md. Rayhan Shaheb Shaheb ◽  
M Mahbuba Begum ◽  
Kabir Uddin Ahmed ◽  
Mahmudul Islam Nazrul ◽  
Siert G Wiersema
Keyword(s):  
Solanum Tuberosum ◽  
Seed Potato ◽  
Production Systems ◽  
Potato Production ◽  
Potato Industry ◽  
Vital Role ◽  
Climatic Conditions ◽  
Supply System ◽  
Policy Implications ◽  
Food And Nutritional Security

Potato (<i>Solanum tuberosum</i> L.) is an important vegetable that plays a vital role in global food and nutritional security and alleviation of poverty, especially in Bangladesh. Although, the soil and climatic conditions of  Bangladesh are favourable for potato cultivation, every year production is hampered due to use of low quality seeds. Seed potato industry is developing in Bangladesh. However, only 5-10% quality seeds are being produced and used by the potato farmers. Quality seeds alone can contribute to increased yield by 15-20%. Both public and private sectors together supply only 5% quality seed potato and the rest 95% is almost of low quality produced by the farmers themselves. In prioritizing different issues of agriculture, challenges of production and supply of quality seed to the farmers should be considered as a top priority issue. For increasing quality seeds, the whole seed system needs to be reviewed. The present work was an attempt to review the seed potato production systems, supply system  and also to identify the possible challenges and policy implications in regards of the problems on seed potato in Bangladesh.The Agriculturists 2015; 13(1) 173-188

scholarly journals Characterization of transgenic potato (Solanum tuberosum) tubers with increased ADPglucose pyrophosphorylase*

1996 ◽  
Vol 320 (2) ◽  
pp. 487-492 ◽  
Author(s):  
Lee J. SWEETLOVE ◽  
Michael M. BURRELL ◽  
Tom ap REES
Keyword(s):  
Solanum Tuberosum ◽  
Starch Synthesis ◽  
Transgenic Potato ◽  
Soluble Starch ◽  
Inorganic Pyrophosphatase ◽  
Cell Fractionation ◽  
Intracellular Location ◽  
Adpglucose Pyrophosphorylase ◽  
Patatin Promoter ◽  
Udpglucose Pyrophosphorylase

The aim of the work described in this paper was to characterize the tubers of potato (Solanum tuberosum var. Prairie) plants that had been transformed with the Escherichiacoli ADPglucose pyrophosphorylase (EC 2.7.7.27) gene, glgC-16, under the control of a patatin promoter. Over 30 lines of transformed plants with increased ADPglucose pyrophosphorylase activity were obtained. The tubers of six of these lines were compared with those of control plants expressing the gene for β-glucuronidase. The average increase in pyrophosphorylase activity was 200%, and the highest was 400%. Western immunoblotting of tuber extracts showed that the amounts of glgC-16 protein were linearly related to the extractable activity of the ADPglucose pyrophosphorylase. Cell fractionation studies showed that the increased activity of the pyrophosphorylase in the glgC-16 tubers had a similar intracellular location, the amyloplast fraction, to that found in the control tubers. No pleiotropic changes in the maximum catalytic activities of the following enzymes could be detected in the glgC-16 tubers: sucrose synthase, fructokinase, UDPglucose pyrophosphorylase, phosphofructokinase, soluble starch synthase, starch branching enzyme, phosphoglucomutase and alkaline inorganic pyrophosphatase. The glgC-16 tubers are held to be suitable for the study of the role of ADPglucose pyrophosphorylase in the control of starch synthesis.

Sign in / Sign up

Export Citation Format

Share Document