Clonal selection in a globe artichoke landrace: characterization of superior germplasm to improve cultivation in Mediterranean environments

2013 ◽  
Vol 153 (1) ◽  
pp. 102-113 ◽  
Author(s):  
R. P. MAURO ◽  
E. PORTIS ◽  
S. LANTERI ◽  
A. LO MONACO ◽  
G. MAUROMICALE
Keyword(s):  
Selection Criteria ◽  
Fragment Length Polymorphism ◽  
Clonal Selection ◽  
Field Performance ◽  
Globe Artichoke ◽  
Weight Yield ◽  
New Varieties ◽  
Primary Selection ◽  
Highly Correlated

SUMMARYThe morphological (using International Union for the Protection of New Varieties of Plants (UPOV) descriptors) and field performance of five clones selected from the globe artichoke landrace Spinoso di Palermo were determined over two seasons, and their amplified fragment length polymorphism (AFLP) profiles detected using seven primer combinations (PCs). The number of heads produced averaged 13·8 per plant (equivalent to a fresh weight yield of 2·1 kg/plant), but two of the clones produced 15·6 heads per plant (2·4 kg/plant). Three clones produced noticeably larger second-order heads (mean of 156 g), and so were considered to be suitable for the production of desirable heads over a prolonged harvesting period. Head yield and the number of heads per plant were associated with a moderate level of broad sense heritability (0·29–0·46), implying that these traits could be viewed as primary selection criteria. From the list of 51 UPOV descriptors, 18 varied among the five clones, but variation at just six, simply scored, descriptors was sufficient to discriminate completely between the examined clones. Full discrimination was also achieved by applying only three of the seven selected AFLP PCs. According to the AFLP profiles, two of the clones were highly similar. The similarity matrices calculated from the UPOV descriptors and the AFLP profiles were highly correlated with one another. The data are optimistic and indicate that the performance of Spinoso di Palermo could be much improved via clonal selection.

scholarly journals Clonal approaches to understanding the impact of mutations on hematologic disease development

Blood ◽  
2019 ◽  
Vol 133 (13) ◽  
pp. 1436-1445 ◽  
Author(s):  
Jyoti Nangalia ◽  
Emily Mitchell ◽  
Anthony R. Green
Keyword(s):  
Somatic Mutations ◽  
Clonal Selection ◽  
Single Cells ◽  
Driver Mutations ◽  
Great Promise ◽  
Tumor Evolution ◽  
Disease Development ◽  
Hematopoietic Tissue ◽  
The Impact

Abstract Interrogation of hematopoietic tissue at the clonal level has a rich history spanning over 50 years, and has provided critical insights into both normal and malignant hematopoiesis. Characterization of chromosomes identified some of the first genetic links to cancer with the discovery of chromosomal translocations in association with many hematological neoplasms. The unique accessibility of hematopoietic tissue and the ability to clonally expand hematopoietic progenitors in vitro has provided fundamental insights into the cellular hierarchy of normal hematopoiesis, as well as the functional impact of driver mutations in disease. Transplantation assays in murine models have enabled cellular assessment of the functional consequences of somatic mutations in vivo. Most recently, next-generation sequencing–based assays have shown great promise in allowing multi-“omic” characterization of single cells. Here, we review how clonal approaches have advanced our understanding of disease development, focusing on the acquisition of somatic mutations, clonal selection, driver mutation cooperation, and tumor evolution.

scholarly journals Doppler evaluation of the fetal pulmonary artery pressure

2019 ◽  
Vol 47 (2) ◽  
pp. 218-221
Author(s):  
Alberto Sosa-Olavarria ◽  
Jesús Zurita-Peralta ◽  
Claudio V. Schenone ◽  
Mauro H. Schenone ◽  
Fernando Prieto
Keyword(s):  
Pulmonary Artery ◽  
Pulmonary Artery Pressure ◽  
Main Pulmonary Artery ◽  
P Value ◽  
Review Of The Literature ◽  
Artery Pressure ◽  
Bivariate Correlation ◽  
Highly Correlated ◽  
Doppler Evaluation

Abstract Background The Doppler effect has allowed the characterization of several vessels in maternal-fetal circulation that have been used for practical purposes. Our review of the literature showed a paucity of information about fetal pulmonary artery pressure (FMPAP) and its behavior in regard to gestational age (GA). The objectives of the study were to evaluate a formula to calculate the main FMPAP and its correlation with GA. Methods A total of 337 fetuses without obvious pathology were studied prospectively using Doppler evaluation of the FMPAP. Using the fetal main pulmonary artery Doppler acceleration time (FMPAT), we obtained the FMPAP using the following formula: FMPAP=90 – (0.62×FMPAT). Regression analyses, Pearson’s bivariate correlation and paired sample t-test were used when appropriate. Results FMPAT increases while FMPAP decreases with GA. Pearson’s correlation coefficient for FMPAP and GA was −0.544 (P-value<0.001) and for FMPAT and GA was 0.556 (P-value<0.001). FMPAP and FMPAT were highly correlated (R=−0.972; P<0.001). Conclusion Pulmonary artery pressure in the fetus decreases with GA.

scholarly journals Shear Strength Characterization of Bauxite Deposits in Kuantan, East Coast Malaysia

2019 ◽  
Vol 8 (3S3) ◽  
pp. 404-407
Keyword(s):  
Shear Strength ◽  
Moisture Content ◽  
East Coast ◽  
Field Performance ◽  
Friction Angle ◽  
Parent Rock ◽  
Index Tests ◽  
Strength Characterization ◽  
The Relationship

Engineering characterization which are useful for "temperate" zone soils usually fail to predict the field performance of bauxitic soils, because the index tests upon which the characterization are based are not always reproducible for bauxitic soils. Fifteen (15) bauxitic soil of undisturbed and disturbed samples from 3 distinct sites in Kuantan, all derived from basalt parent rock but representing various stages of weathering were subjected to engineering and mineralogic tests. Values for cohesion and friction angles are evaluated. Soils from Semambu has the highest moisture content of 33.27%, the cohesion value is however lower compared to Bukit Goh which has moisture content of 21.74%. Study are further done to discover the relationship with cohesion and friction angles. Thus, by measuring the cohesion and friction angle can evaluate the performance of bauxite shear strength.

Characterization of DR blank alleles by restriction fragment length polymorphism (RFLP)

2008 ◽  
Vol 29 (3) ◽  
pp. 154-159 ◽  
Author(s):  
E. Keller ◽  
A. Andreas-Zietz ◽  
A. McNicholas ◽  
A. Grooms ◽  
S. Scholz ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Fragment Length

Assessment of Genetic Diversity of Rheum species (Endangered Medicinal Herb of Indian Himalayan Region) using Molecular Markers

2021 ◽  
Vol 16 (11) ◽  
pp. 147-154
Author(s):  
Anjali Uniyal ◽  
Akhilesh Kumar ◽  
Sweta Upadhyay ◽  
Vijay Kumar ◽  
Sanjay Gupta
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Fragment Length Polymorphism ◽  
Dna Polymorphisms ◽  
Inter Simple Sequence Repeats ◽  
Pharmaceutical Industries ◽  
Endangered Medicinal Herb ◽  
Identification And Characterization ◽  
Simple Sequence

The Rheum species are important medicinal plants that are facing extinction due to their unplanned development and overexploitation by pharmaceutical industries. DNA polymorphisms are not prone to environmental modifications, thus they are widely used for the identification and characterization of plants. The use of different molecular markers has enabled the researchers for the valuation of genetic variability and diversity in its natural zone of distribution. The conventional approach may take several years to yield this information. For the estimation of molecular and genetic variations in geographical zone of distribution, various molecular markers technique are available like RAPD (Randomly Amplified Polymorphic DNA), RFLP (Restriction fragment length polymorphism), ISSR (Inter-Simple Sequence Repeats), SSR and AFLP. The uses of different molecular markers for the study of genetic diversity have been discussed in the review.

scholarly journals Generation of Glioblastoma Patient-Derived Intracranial Xenografts for Preclinical Studies

2020 ◽  
Vol 21 (14) ◽  
pp. 5113
Author(s):  
Amber E. Kerstetter-Fogle ◽  
Peggy L. R. Harris ◽  
Susann M. Brady-Kalnay ◽  
Andrew E. Sloan
Keyword(s):  
Cultured Cells ◽  
Clonal Selection ◽  
Previous Treatment ◽  
Growth Patterns ◽  
Phenotypic Characterization ◽  
Genomic Signatures ◽  
Culture Model ◽  
Passage Number ◽  
Human Gbm

Glioblastoma multiforme (GBM) is the most malignant primary brain cancer affecting adults. Therapeutic options for GBM have remained the same for over a decade with no significant improvement. Many therapies that are successful in culture have failed in patients, likely due to the complex microenvironment in the brain, which has yet to be reproduced in any culture model. Furthermore, the high passage number of cultured cells and clonal selection fail to recapitulate the molecular and genomic signatures of GBM. We have established orthotopic patient-derived xenografts (PDX) from 37 GBM patients with human GBM. Of the 69 patient samples analyzed, we were successful in passaging 37 lines three or more generations (53.6%). After phenotypic characterization of the xenografted tumor tissue, two different growth patterns emerged highly invasive or localized. The phenotype was dependent on malignancy and previous treatment of the patient from which the xenograft was derived. Physiologically, mice exhibited symptoms more quickly with each subsequent passage, particularly in the localized tumors. Study of these physiologically relevant human xenografts in mice will enable therapeutic screenings in a microenvironment that more closely resembles GBM and may allow development of individualized patient models which may eventually be used for simulating treatment.

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