scholarly journals Screening of wild oil palm (Elaeis guineensis) germplasm for lipase activity

2015 ◽  
Vol 154 (7) ◽  
pp. 1241-1252 ◽  
Author(s):  
Y. T. WONG ◽  
A. KUSHAIRI ◽  
N. RAJANAIDU ◽  
M. OSMAN ◽  
R. WICKNESWARI ◽  
...  
Keyword(s):  
Sierra Leone ◽  
Lipase Activity ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Quantitative Polymerase Chain Reaction ◽  
Oil Quality ◽  
The Novel ◽  
Novel Method ◽  
Polymerase Chain ◽  
Titration Assay

SUMMARYPalm oil comprises mainly triacylglycerols (TAG) that account for about 0·95 of its composition. Lipolysis of TAG releases free fatty acids that are then oxidized, impairing oil quality. Lower endogenous lipase activity is therefore desirable for better quality oil. Identifying genotypes for this should allow production of oils with improved shelf-life. A novel non-radioactive cold-activation titration assay was used to assay for lipase activity in 49 randomly selected Elaeis guineensis palms using the novel method as well as the traditional radioactive method. The results confirmed significant correlation between the radioactive and novel non-radioactive methods. A total of 148 E. guineensis palms originating from Angola, Cameroon, Guinea Conarky, Nigeria, Senagal, Sierra Leone, Tanzania and Zaire were then screened for lipase activity using the novel non-radioactive method. The analysis revealed genotypes with significantly different lipase activities, indicating that the activity is genotype-dependent. Quantitative polymerase chain reaction indicated similarity in pattern between gene expression and lipase activity determined by the novel assay. Several low and high lipase genotypes were identified. The findings pave the way for selection and breeding for low lipase genotypes. They are also relevant to genetic marker development for oil quality. Evidence is presented for the presence of at least two different lipases that determine oil quality in the oil palm mesocarp.

Pseudospiropes elaeidis. [Distribution map].

1989 ◽  
Author(s):  
Keyword(s):  
Sierra Leone ◽  
Geographical Distribution ◽  
Oil Palm ◽  
Ivory Coast ◽  
Elaeis Guineensis ◽  
Distribution Map ◽  
Guinea Bissau ◽  
New Distribution

Abstract A new distribution map is provided for Pseudospiropes elaeidis (Steyaert) Deighton. Hosts: Oil palm (Elaeis guineensis). Information is given on the geographical distribution in Africa, Angola, Benin, Cameroon, Congo, Gabon, Ghana, Guinea, Guinea-Bissau, Ivory Coast, Nigeria, Sao Tome, Senegal, Sierra Leone, Sudan, Tanzania, Togo, Zaire.

Cercospora elaeidis. [Distribution map].

1972 ◽  
Author(s):  
Keyword(s):  
Sierra Leone ◽  
Geographical Distribution ◽  
Oil Palm ◽  
Ivory Coast ◽  
Elaeis Guineensis ◽  
Distribution Map ◽  
New Distribution

Abstract A new distribution map is provided for Cercospora elaeidis Steyaert. Hosts: Oil palm (Elaeis guineensis). Information is given on the geographical distribution in AFRICA, Angola, Cameroon, Congo Republic, Dahomey, Ghana, Guinea, Ivory Coast, Nigeria, Portuguese Guinea, Rhodesia, Sao Tomé, Senegal, Sierra Leone, Sudan, Tanzania, Zaire Republic (Congo).

Diagnosis and follow-up of Bartonella henselae infection in the spleen of an immunocompetent patient by real-time quantitative PCR

2013 ◽  
Vol 62 (7) ◽  
pp. 1081-1085 ◽  
Author(s):  
Maria Carla Liberto ◽  
Giovanni Matera ◽  
Angelo G. Lamberti ◽  
Angela Quirino ◽  
Giorgio S. Barreca ◽  
...  
Keyword(s):  
Real Time ◽  
Bartonella Henselae ◽  
Quantitative Polymerase Chain Reaction ◽  
Immunocompetent Patient ◽  
Chain Reaction ◽  
Real Time Quantitative Pcr ◽  
Novel Method ◽  
Polymerase Chain ◽  
Immunocompetent Adults

Systemic Bartonella henselae infections are unusual in immunocompetent adults. However, here we report one such case of bartonellosis in a 34-year-old patient, who presented with fever and multinodular splenomegaly. We also describe a novel method of identifying Bartonella henselae by real-time quantitative polymerase chain reaction and sequencing of amplified products. This could prevent splenic bartonellosis being mistaken for lymphoma and thereby avert unnecessary splenectomy.

scholarly journals Screening of wild oil palm (Elaeis guineensis) germplasm for lipase activity – CORRIGENDUM

2016 ◽  
Vol 154 (7) ◽  
pp. 1253-1253
Author(s):  
Y. T. WONG ◽  
A. KUSHAIRI ◽  
N. RAJANAIDU ◽  
M. OSMAN ◽  
R. WICKNESWARI ◽  
...  
Keyword(s):  
Lipase Activity ◽  
Oil Palm ◽  
Elaeis Guineensis

scholarly journals Brief Review on the Controversies around Oil Palm (Elaeis Guineensis Jacq.) Production and Palm Oil Consumption

2016 ◽  
Vol 3 (2) ◽  
pp. 60 ◽  
Author(s):  
Godswill Ntsomboh Ntsefong ◽  
Hermine Ngalle-Bille ◽  
Walter Ajambang ◽  
Benoit Constant Likeng-Li-Ngue ◽  
Tabi-Mbi Kingsley ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Palm Oil ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Saturated Fatty Acids ◽  
Oil Quality ◽  
Human Consumption ◽  
Oil Consumption ◽  
Oil Palm Industry ◽  
Environmentally Friendly Production

Palm oil from <em>Elaeis guineensis</em> Jacq. of the Arecaceae family is the main traditional cooking oil in most parts of tropical Africa. Oil palm cultivation requires large areas of land and the consumption of crude palm oil from it raises health concerns notably with regards to the incidence of cardiovascular disease and obesity. The industry has therefore been faced with two major controversies. Firstly, palm oil which contains about 50% saturated fatty acids is considered to be unhealthy for human consumption because its saturated fatty acids raise blood LDL cholesterol level and increase the risk of the above mentioned diseases. Secondly, due to its land intensive nature, the oil palm industry is linked to deforestation, climate change and socioeconomic instability. For these reasons, the oil palm industry has been at the forefront of environmental and reputational challenges. This paper briefly presents an overview of these controversies surrounding the oil palm industry, while highlighting the possible outlets for environmentally friendly production processes and improvement strategies for better oil quality.

scholarly journals High-Oleic Palm Oil (HOPO) Production from Parthenocarpic Fruits in Oil Palm Interspecific Hybrids Using Naphthalene Acetic Acid

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 290
Author(s):  
Hernán Mauricio Romero ◽  
Edison Daza ◽  
Iván Ayala-Díaz ◽  
Rodrigo Ruiz-Romero
Keyword(s):  
Acetic Acid ◽  
Palm Oil ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Fatty Acid Content ◽  
Oil Quality ◽  
Naphthalene Acetic Acid ◽  
Elaeis Oleifera ◽  
High Oleic ◽  
Seedless Fruits

Interspecific OxG hybrids of African palm Elaeis guineensis Jacq. and the American palm Elaeis oleifera Cortes produce high-oleic palm oil (HOPO) with low saturated fatty acid content. OxG hybrids are highly productive, grow slowly, and are resistant to bud rot disease. However, OxG hybrid pollen presents low viability and germinability, so assisted pollination is a must. Hybrids can produce parthenocarpic or seedless fruits, with the exogenous application of plant growth regulators. Thus, naphthalene acetic acid (NAA) effects on parthenocarpic fruits induction, bunch formation, and oil quality were evaluated. The OxG hybrid Coari x La Mé was used. NAA doses, frequency, number of applications, and the phenological stages for the treatments were defined. A total dose of 1200 mg L−1 NAA applied three or four times produced bunches with better fruit set, similar average bunch weight, and oil to dry mesocarp than those obtained with assisted pollination. At a semi-commercial scale, 1200 mg L−1 NAA induced bunches that consisted of 93% or more of seedless fruits. Bunch number (2208 ± 84 versus 1690 ± 129) and oil to bunch (32.2 ± 0.7 versus 25.3 ± 0.8) were higher in the NAA induced bunches than in the assisted pollination. However, the average bunch weight was lower (12.2 ± 0.4 versus 14.9 ± 0.6). NAA increased oil to bunch in 36% (8.7 ± 0.1 versus 6.4 ± 0.3). Thus, with this technology, it is plausible to reach more than 10 tons per hectare per year of HOPO. Potentially, without increasing the planted oil palm area, OxG hybrids and NAA applications could alone meet the world’s fats and oil demands.

scholarly journals DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Nina Unzila Angkat ◽  
LUTHFI AZIZ MAHMUD SIREGAR ◽  
Mohammad Basyuni ◽  
Dadang Afandi ◽  
INDRA SYAHPUTRA
Keyword(s):  
Genetic Diversity ◽  
Lipase Activity ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Edible Oil ◽  
Lipase Gene ◽  
Analysis Of Molecular Variance ◽  
Expected Heterozygosity ◽  
Activity Groups ◽  
Informative Content

Abstract. Angkat NU, Siregar LAM, Basyuni M, Afandi D, Syahputra I. 2021. DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line. Biodiversitas 22: 900-905. The acidification of palm oil due to lipase activity in the mesocarp is assessed under genetic control. Three molecular markers have been established to gauge the lipase gene in oil palm. Lower lipase activity is desired for good quality edible oil. This study aims to identify the genetic diversity by screening groups/families to determine an elite low lipase genotype of oil palm. Genetic diversity and population structure of 15 groups of oil palm were investigated by using three specific markers with GenAlex 6.502 software. Results show that the Polymorphic Informative Content (PIC) value of markers was around 0,985-0,993, which indicates that these markers are effective in determining the diversity of lipase activity in oil palm. Analysis of molecular variance (AMOVA) revealed that genetic diversity varies within individuals (54%), among individuals (31%), and among population (15%). The value of number of alleles (Na), number of effective alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and number of allele migration (Nm) indicate that the genetic diversity in this population is relatively low. Phylogenetic analysis identified two main groups as high lipase and low lipase activity groups based on DNA intensity.

scholarly journals Association Mapping Between Candidate Gene SNP and Production and Oil Quality Traits in Interspecific Oil Palm Hybrids

Plants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 377 ◽  
Author(s):  
Astorkia ◽  
Hernandez ◽  
Bocs ◽  
Armentia ◽  
Herran ◽  
...  
Keyword(s):  
Association Mapping ◽  
Candidate Gene ◽  
Oil Palm ◽  
Linear Models ◽  
Elaeis Guineensis ◽  
Association Studies ◽  
Oil Quality ◽  
Type I ◽  
Quality Traits ◽  
P Values

Oil palm production is gaining importance in Central and South America. However, the main species Elaeis guineensis (Eg) is suffering severely from bud rod disease, restricting the potential cultivation areas. Therefore, breeding companies have started to work with interspecific Elaeis oleifera × Eg (Eo × Eg) hybrids which are tolerant to this disease. We performed association studies between candidate gene (CG) single nucleotide polymorphisms (SNP) and six production and 19 oil quality traits in 198 accessions of interspecific oil palm hybrids from five different origins. For this purpose, barcoded amplicons of initially 167 CG were produced from each genotype and sequenced with Ion Torrent. After sequence cleaning 115 SNP remained targeting 62 CG. The influence of the origins on the different traits was analyzed and a genetic diversity study was performed. Two generalized linear models (GLM) with principle component analysis (PCA) or structure (Q) matrixes as covariates and two mixed linear models (MLM) which included in addition a Kinship (K) matrix were applied for association mapping using GAPIT. False discovery rate (FDR) multiple testing corrections were applied in order to avoid Type I errors. However, with FDR adjusted p values no significant associations between SNP and traits were detected. If using unadjusted p values below 0.05, seven of the studied CG showed potential associations with production traits, while 23 CG may influence different quality traits. Under these conditions the current approach and the detected candidate genes could be exploited for selecting genotypes with superior CG alleles in Marker Assisted Selection systems.

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