Screening of wild oil palm (Elaeis guineensis) germplasm for lipase activity

Abstract. Angkat NU, Siregar LAM, Basyuni M, Afandi D, Syahputra I. 2021. DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line. Biodiversitas 22: 900-905. The acidification of palm oil due to lipase activity in the mesocarp is assessed under genetic control. Three molecular markers have been established to gauge the lipase gene in oil palm. Lower lipase activity is desired for good quality edible oil. This study aims to identify the genetic diversity by screening groups/families to determine an elite low lipase genotype of oil palm. Genetic diversity and population structure of 15 groups of oil palm were investigated by using three specific markers with GenAlex 6.502 software. Results show that the Polymorphic Informative Content (PIC) value of markers was around 0,985-0,993, which indicates that these markers are effective in determining the diversity of lipase activity in oil palm. Analysis of molecular variance (AMOVA) revealed that genetic diversity varies within individuals (54%), among individuals (31%), and among population (15%). The value of number of alleles (Na), number of effective alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and number of allele migration (Nm) indicate that the genetic diversity in this population is relatively low. Phylogenetic analysis identified two main groups as high lipase and low lipase activity groups based on DNA intensity.

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The Journal of Agricultural Science ◽

10.1017/s0021859615001112 ◽

2015 ◽

Vol 154 (7) ◽

pp. 1241-1252 ◽

Cited By ~ 5

Author(s):

Y. T. WONG ◽

A. KUSHAIRI ◽

N. RAJANAIDU ◽

M. OSMAN ◽

R. WICKNESWARI ◽

...

Keyword(s):

Sierra Leone ◽

Lipase Activity ◽

Oil Palm ◽

Elaeis Guineensis ◽

Quantitative Polymerase Chain Reaction ◽

Oil Quality ◽

The Novel ◽

Novel Method ◽

Polymerase Chain ◽

Titration Assay

SUMMARYPalm oil comprises mainly triacylglycerols (TAG) that account for about 0·95 of its composition. Lipolysis of TAG releases free fatty acids that are then oxidized, impairing oil quality. Lower endogenous lipase activity is therefore desirable for better quality oil. Identifying genotypes for this should allow production of oils with improved shelf-life. A novel non-radioactive cold-activation titration assay was used to assay for lipase activity in 49 randomly selected Elaeis guineensis palms using the novel method as well as the traditional radioactive method. The results confirmed significant correlation between the radioactive and novel non-radioactive methods. A total of 148 E. guineensis palms originating from Angola, Cameroon, Guinea Conarky, Nigeria, Senagal, Sierra Leone, Tanzania and Zaire were then screened for lipase activity using the novel non-radioactive method. The analysis revealed genotypes with significantly different lipase activities, indicating that the activity is genotype-dependent. Quantitative polymerase chain reaction indicated similarity in pattern between gene expression and lipase activity determined by the novel assay. Several low and high lipase genotypes were identified. The findings pave the way for selection and breeding for low lipase genotypes. They are also relevant to genetic marker development for oil quality. Evidence is presented for the presence of at least two different lipases that determine oil quality in the oil palm mesocarp.

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Assaying lipase activity from oil palm fruit (Elaeis guineensis Jacq.) mesocarp

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2006.09.006 ◽

2006 ◽

Vol 44 (10) ◽

pp. 611-617 ◽

Cited By ~ 39

Author(s):

G.F. Ngando Ebongue ◽

R. Dhouib ◽

F. Carrière ◽

P.-H. Amvam Zollo ◽

V. Arondel

Keyword(s):

Lipase Activity ◽

Oil Palm ◽

Elaeis Guineensis ◽

Palm Fruit ◽

Elaeis Guineensis Jacq

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Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d220237 ◽

2021 ◽

Vol 22 (2) ◽

Author(s):

TENGKU SITI HABSYAH ◽

Mohammad Basyuni ◽

LUTHFI A.M. SIREGAR ◽

DADANG AFANDI ◽

INDRA SYAHPUTRA

Keyword(s):

Total Lipid ◽

Lipase Activity ◽

Oil Palm ◽

Elaeis Guineensis ◽

Leaf Tissue ◽

Dry Weight ◽

Carbon Chain ◽

Chain Lengths ◽

North Sumatra ◽

Palm Leaf

Abstract. Habsyah TS, Basyuni M, Siregar LAM, Afandi D, Syahputra I. 2021. Distribution of polyprenol and dolichol in oil palm genotype (Elaeis guineensis) involving lipase activity. Biodiversitas 22: 830-837. The aim of this study was to determine the pattern of chemical compounds from the polyisoprenoid group in oil palm plants that were contributing to the lipase activity. The study was conducted in two locations. A sampling of leaves and fruit mesocarp from 90 progenies was conducted at PT. Socfin Indonesia Aek Loba Plantation, Asahan District, North Sumatra, Indonesia. Biochemical analysis of polyisoprenoid was carried out at the Forest Biotechnology Laboratory, Faculty of Forestry, University of Sumatera Utara, Medan. The results showed that the total lipids in leaves tissue ranged from 0.40 to 1.032 mg/g, with the lowest total lipid found in SL-4966 progeny and the highest total lipid found in SL-9737 progeny. Polyisoprenoid values ranged from 1.63 to 10.82 mg/g dry weight. The highest polyisoprenoid content was obtained in SL-10682 progeny, and the lowest was in SL-5045 progeny leaf tissue. The polyisoprenoid in fruit mesocarp tissue ranged from 1.79 to 4.14 mg/g. The lowest polyisoprenoid content (1.79 mg/g) of fruit mesocarp was found in SL-4233 progeny, while the highest (4.14 mg/g) was found in SL-5010 progeny. Dolichol and polyprenol are equally dominant in palm leaves. Carbon polyprenol chain lengths ranged from C45-C105, and dolichol ranged from C50-C110. The polyprenol carbon chain lengths in the mesocarp tissues were C65-C120 and dolichol was C65-C125. Polyisoprenoid analysis on oil palm leaf and fruit mesocarp tissue showed that lipid patterns of oil palm leaf and mesocarp were categorized as type II lipid patterns. It provides important information for selecting genotypes of low lipase activity of oil palm plants.

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Factors Affecting Lipase Activity in the Oil Palm (Elaeis Guineensis) Mesocarp

Plant Lipid Metabolism ◽

10.1007/978-94-015-8394-7_155 ◽

1995 ◽

pp. 555-557 ◽

Cited By ~ 5

Author(s):

Ravigadevi Sambanthamurthi ◽

Oo Khaik Cheang ◽

Siti Hasnah Parman

Keyword(s):

Lipase Activity ◽

Oil Palm ◽

Elaeis Guineensis ◽

Factors Affecting

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Effect of chemicals on disintegration of the operculum in oil palm (Elaeis guineensis) seeds for early germination

Seed Science and Technology ◽

10.15258/sst.2016.44.3.16 ◽

2016 ◽

Vol 44 (3) ◽

pp. 475-485

Author(s):

G. Ravichandran ◽

P. Murugesan ◽

P. Naveen Kumar ◽

R.K. Mathur ◽

D. Ramajayam

Keyword(s):

Oil Palm ◽

Elaeis Guineensis ◽

Early Germination

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The chitinase activity of oil palm (Elaeis guineensis Jacq.) roots against fungal endophytes and pathogenic Ganoderma boninense

Plant Omics ◽

10.21475/poj.10.05.17.pne853 ◽

2017 ◽

Vol 10 (05) ◽

pp. 247-251 ◽

Cited By ~ 2

Author(s):

Yurnaliza ◽

◽

Rizkita Rachmi Esyanti ◽

Agus Susanto ◽

I Nyoman Pugeg Aryantha ◽

...

Keyword(s):

Oil Palm ◽

Elaeis Guineensis ◽

Fungal Endophytes ◽

Chitinase Activity ◽

Ganoderma Boninense ◽

Elaeis Guineensis Jacq

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LEGITIMACY OF PROGENIES USING SSR MARKERS AS CONTROLLING SYSTEM AND EARLIER SELECTION IN THE OIL PALM NURSERY (Elaeis guineensis JACQ.)

Jurnal Penelitian Kelapa Sawit ◽

10.22302/10.22302/iopri.jur.jpks.v25i1.22 ◽

2018 ◽

Vol 25 (1) ◽

pp. 21-30

Author(s):

Rokhana Faizah ◽

Sri Wening ◽

Abdul Razak Purba

Keyword(s):

Dna Fingerprinting ◽

Ssr Markers ◽

Oil Palm ◽

Dna Markers ◽

Elaeis Guineensis ◽

Gene Marker ◽

Controlling System ◽

Crossing Probability ◽

Simple Sequence

Information of legitimacy of oil palm progenies is important to guaranty the quality and to control commercial seeds procedures. A true and legitimate cross will produce progeny which has a combination of their parent's allele. The information could be obtained early in the nursery stage through DNA fingerprinting analysis. Simple Sequence Repeats (SSR) is one of DNA markers used for DNA fingerprinting, since the marker system has advantages to acquire information of allele per individual in population and efficiency diverse allele of progeny and their parents. The aim of the research is to obtain legitimacy of 12 progenies analyzing in the oil palm nursery stage. Thirteen SSR markers were used to analyze 12 crossings number of oil palm. The genotypes data by alleles of SSR inferred and quantified using Gene Marker® Software version 2.4.0 Soft Genetics® LLC and analyzed based on Mendel's Law of Segregation. The result showed based on heredity pattern of progeny and their parent's allele that progenies H were indicated genetically derived from their known parents while progenies from A and G indicated as illegitimate crossing. Probability value for legitimacy of progenies of 9 other crosses has 0.031 and 0.5. Legitimacy analysis of progeny using SSR markers could be used to control the quality of crossing material and earlier selection in the oil palm nursery.

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Impact of oil palm (Elaeis guineensis Jacq.) cultivation on some chemical and microbiological properties of an ultisol

Biology and Fertility of Soils ◽

10.1007/bf00256992 ◽

1987 ◽

Vol 4 (3) ◽

Author(s):

N.O. Aisueni

Keyword(s):

Oil Palm ◽

Elaeis Guineensis ◽

Elaeis Guineensis Jacq ◽

Microbiological Properties

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EgJUB1 and EgERF113 transcription factors as potential master regulators of defense response in Elaeis guineensis against the hemibiotrophic Ganoderma boninense

BMC Plant Biology ◽

10.1186/s12870-020-02812-7 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Nurshafika Mohd Sakeh ◽

Siti Nor Akmar Abdullah ◽

Mohammad Nazri Abdul Bahari ◽

Azzreena Mohamad Azzeme ◽

Noor Azmi Shaharuddin ◽

...

Keyword(s):

Transcription Factors ◽

Oil Palm ◽

Defense Response ◽

Fungal Pathogen ◽

Defense Mechanism ◽

Elaeis Guineensis ◽

Expression Profiles ◽

Present Report ◽

Ganoderma Boninense ◽

Cell Wall Modification

Abstract Background Hemibiotrophic pathogen such as the fungal pathogen Ganoderma boninense that is destructive to oil palm, manipulates host defense mechanism by strategically switching from biotrophic to necrotrophic phase. Our previous study revealed two distinguishable expression profiles of oil palm genes that formed the basis in deducing biotrophic phase at early interaction which switched to necrotrophic phase at a later stage of infection. Results The present report is a continuing study from our previous published transcriptomic profiling of oil palm seedlings against G. boninense. We focused on identifying differentially expressed genes (DEGs) encoding transcription factors (TFs) from the same RNA-seq data; resulting in 106 upregulated and 108 downregulated TFs being identified. The DEGs are involved in four established defense-related pathways responsible for cell wall modification, reactive oxygen species (ROS)-mediated signaling, programmed cell death (PCD) and plant innate immunity. We discovered upregulation of JUNGBRUNNEN 1 (EgJUB1) during the fungal biotrophic phase while Ethylene Responsive Factor 113 (EgERF113) demonstrated prominent upregulation when the palm switches to defense against necrotrophic phase. EgJUB1 was shown to have a binding activity to a 19 bp palindromic SNBE1 element, WNNYBTNNNNNNNAMGNHW found in the promoter region of co-expressing EgHSFC-2b. Further in silico analysis of promoter regions revealed co-expression of EgJUB1 with TFs containing SNBE1 element with single nucleotide change at either the 5th or 18th position. Meanwhile, EgERF113 binds to both GCC and DRE/CRT elements promoting plasticity in upregulating the downstream defense-related genes. Both TFs were proven to be nuclear-localized based on subcellular localization experiment using onion epidermal cells. Conclusion Our findings demonstrated unprecedented transcriptional reprogramming of specific TFs potentially to enable regulation of a specific set of genes during different infection phases of this hemibiotrophic fungal pathogen. The results propose the intricacy of oil palm defense response in orchestrating EgJUB1 during biotrophic and EgERF113 during the subsequent transition to the necrotrophic phase. Binding of EgJUB1 to SNBE motif instead of NACBS while EgERF113 to GCC-box and DRE/CRT motifs is unconventional and not normally associated with pathogen infection. Identification of these phase-specific oil palm TFs is important in designing strategies to tackle or attenuate the progress of infection.

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