166. The relative values of raw and pasteurized milk in the feeding of calves

1937 ◽  
Vol 8 (3) ◽  
pp. 311-323 ◽  
Author(s):  
J. Wilkie ◽  
S. J. Edwards ◽  
A. B. Fowler ◽  
N. C. Wright
Keyword(s):  
Guinea Pigs ◽  
Nutritive Value ◽  
Live Weight ◽  
Raw Milk ◽  
Bulk Sample ◽  
Tuberculous Infection ◽  
Post Mortem ◽  
Pasteurized Milk ◽  
Post Mortem Examination ◽  
The Difference

1. Bull calves from tuberculin-tested Ayrshire herds were fed on raw or commercially pasteurized milk up to 12 weeks of age, in amounts strictly in relation to their body weight. This diet was supplemented from the eighth week by hay at the rate of 2/3 lb. per head per day.The milk used was mixed milk from untested herds, the raw and pasteurized milk being derived from the same bulk sample. The quality of the milk was controlled by daily butterfat and phosphatase tests and by inoculation of grouped daily aliquots twice weekly into duplicate guinea-pigs.2. The total number of calves used in the experiment was ninety-two. Of these, forty-two received pasteurized milk and fifty raw milk. Nineteen calves died before completion of the experiment, five in the pasteurized milk-fed group and fourteen in the raw milk-fed group. The mean ages of these calves at death were 44 and 27 days respectively.Seventy-three calves completed the full 12 weeks of the experimental feeding. Of these, thirty-seven received pasteurized milk and thirty-six raw milk. The former group showed a mean percentage gain in live weight of 80·2 ± 3·1, the corresponding figure for the latter group being 74·5 ± 2·7. The difference, i.e. 5·7 + 4·1, was not significant. Reasons are given for the relatively low growth rate observed.3. No appreciable differences were noted in the skeletal growth of the two groups. Marks awarded by experienced stock judges showed consistent differences in favour of the pasteurized milk-fed group, although the significance of such differences cannot be assessed.4. Inoculations of grouped daily aliquots of raw milk twice weekly into duplicate guinea-pigs resulted in finding viable tubercle bacilli in 70% of the samples and Br. abortus in 38% of them. The pasteurized milk samples were uniformly negative to both tests.The differences in tuberculous infection of the two types of milk were reflected in the results of tuberculin tests and post-mortem examinations on the calves at the conclusion of the experiment. Twenty-four out of thirty-six calves fed on raw milk reacted to the test, and the presence of tuberculous lesions was confirmed in twenty-three by post-mortem examination. One calf in the pasteurized milk-fed group reacted to the test, but exhaustive post-mortem examination and inoculation of glandular material into guineapigs failed to confirm the presence of any tuberculosis.5. This work has failed to show any significant differences in the nutritive value of raw and of pasteurized milk for the rearing of young calves. The use of pasteurized milk, however, had a clear advantage in that it preserved the animals from infection through drinking milk containing living tubercle bacillli.

scholarly journals EXPERIMENTAL EPIDEMIOLOGY OF TUBERCULOSIS

1930 ◽  
Vol 51 (5) ◽  
pp. 769-776 ◽  
Author(s):  
Max B. Lurie
Keyword(s):  
Guinea Pigs ◽  
Wire Mesh ◽  
The Other ◽  
Tuberculous Infection ◽  
Source Of Infection ◽  
Natural Modes ◽  
The Difference ◽  
The One ◽  
Portal Of Entry ◽  
Human Autopsy Material

Under conditions closely simulating the natural modes of tuberculous infection in man normal guinea pigs have acquired tuberculosis by being exposed under two degrees of crowding to tuberculous cage mates in ordinary cages, where the food became soiled with excreta, bearing tubercle bacilli, and in special cages, with wire-mesh floors, where this source of infection was almost entirely eliminated. Guinea pigs were also exposed in the same room but not in the same cage with tuberculous animals. It was found that the relative tuberculous involvement of the mesenteric and tracheobronchial nodes showed a gradation of change from an almost completely alimentary infection to a completely respiratory infection. The disease involved the mesenteric nodes predominantly in the crowded ordinary cages, with much less or no affection of the tracheobronchial nodes. It was similarly, but less markedly, enteric in origin in the less crowded ordinary cages, the mesenteric nodes again being larger than the tracheobronchial nodes, but the difference in size was not so great. In the more crowded special cages the relative affection of these two groups of nodes alternated, so that in some the mesenteric, in some the tracheobronchial nodes were more extensively tuberculous. A disease characterized by less or no affection of the mesenteric nodes and by extensive lesions of the tracheobronchial nodes was seen in the less crowded special cages. Finally there was a massive tuberculosis of the tracheobronchial nodes with usually no affection of the mesenteric nodes in the frankly air-borne tuberculosis acquired by guinea pigs exposed in the same room but not to tuberculous cage mates. This gradation in the rô1e played by the enteric and respiratory routes of infection, as first the one and then the other becomes the more frequent channel of entrance for tuberculosis, would indicate that the penetration of tubercle bacilli by the one portal of entry inhibits the engrafting of tuberculosis in the tissues by way of the other portal of entry. It is apparent that in the special cages the opportunities for inhaling tubercle bacilli are at most equal to if not much less than in the ordinary cages; for in the latter dust from the bedding, laden with tubercle bacilli, is stirred up almost constantly by the animals, whereas in the special cages there is no bedding at all, and therefore, presumably, no more tubercle bacilli in the air than may occur in any part of the room. Nevertheless the route of infection was predominantly the respiratory tract in the special cages, especially in the less crowded, apparently because the enteric route had been largely eliminated. The greater predominance of the respiratory route amongst guinea pigs that acquired tuberculosis in the less crowded ordinary cages as compared to the lesser significance of this route in the more crowded ordinary cages would point in the same direction. These observations are in harmony with our knowledge that tuberculosis once implanted in an organism confers a certain degree of immunity to the disease. It is noteworthy that in a study of human autopsy material Opie (3) has found that when healed lesions are present in the mesentery focal tuberculosis in the lungs is seldom found, and that when first infection occurs by way of the lungs it tends to prevent the engrafting of the disease by way of the intestinal tract.

scholarly journals The proportional frequency of the human and bovine types of tubercle bacilli in human pulmonary tuberculosis in the middle and south of Scotland

1940 ◽  
Vol 40 (3) ◽  
pp. 365-376 ◽  
Author(s):  
A. Stanley Griffith
Keyword(s):  
Pulmonary Tuberculosis ◽  
Thoracic Spine ◽  
Guinea Pigs ◽  
Direct Contact ◽  
Spinal Tuberculosis ◽  
Raw Milk ◽  
Tubercle Bacillus ◽  
Post Mortem ◽  
Cervical Gland ◽  
Gland Enlargement

1. The types of tubercle bacilli have been determined in the sputum of 515 cases of pulmonary tuberculosis occurring in the middle and south of Scotland.2. Of the 515 cases 484 were human (476 eugonic and eight dysgonic) and thirty-one were bovine infections.3. With the exception of the strains from one case (case 28) all the bovine strains, seventy in number, were typical culturally and fully virulent for rabbits.4. The attenuated strains, two in number, from case 28 were slightly less virulent than typical bovine strains for rabbits and (one strain) for guinea-pigs.5. The percentage of bovine infections found in this series, including the Cumberland case, during the years 1931–9 was 6·0, but excluding that case it was 5·8.6. The percentage of bovine infections found by Munro during about the same period and covering the same regions was 5·0%.7. In Munro's series strains of bovine tubercle bacilli were obtained from fifty-eight out of 1165 persons (5·0%). Five of his cases yielded attenuated bovine strains and in one of these the pulmonary tuberculosis was preceded by tuberculosis of the thoracic spine.8. In my series the attenuated tubercle bacilli came from a case (case 28) of pulmonary tuberculosis which was preceded nearly 20 years previously by tuberculosis of the lower dorsal spine.Dr Munro and others have made post-mortem examinations on cases of phthisis pulmonalis due to bovine bacilli, but I wish to defer reference to these until we can review them altogether.In this series there are seven instances of cervical gland enlargement and one instance (case 28) of spinal tuberculosis occurring previous to the development of phthisis pulmonalis. These, I think, are examples of alimentary infection with the bovine tubercle bacillus. Thus, with the three autopsies previously mentioned, there are eleven cases, or about one-third, which are almost certainly alimentary in origin. As for the rest of the cases, 20 in number, no glandular enlargements in neck or abdomen were detected but the majority, if not all, were probably alimentary in origin, since all the persons drank a lot of raw milk and only five came into direct contact with cattle in their employment.

Restricted and ad lib. feeding of liquid skim-milk, with and without the addition of formalin, to fattening pigs

1960 ◽  
Vol 27 (1) ◽  
pp. 103-114 ◽  
Author(s):  
K. G. Mitchell ◽  
P. H. Sedgwick
Keyword(s):  
Nutritive Value ◽  
Skim Milk ◽  
Live Weight ◽  
Food Utilization ◽  
Eye Muscle ◽  
Warm Weather ◽  
Daily Allowance ◽  
Fat Thickness ◽  
The Difference ◽  
Fattening Pigs

SummaryResults are reported of an experiment with fattening pigs, made under commercial conditions, on the effect of adding formalin to liquid skim-milk, to delay souring, on the nutritive value of the milk given either in restricted or in unrestricted quantities.There were four treatments: (i) Meal, fed to a scale based on live weight, plus sour skim-milk restricted to a maximum of 5¼ pints per pig per day. (ii) As (i) except that milk kept sweet by the addition of 0·15% formalin shortly after separation, was fed. (iii) Daily allowance of 2 lb meal/pig fed throughout the fattening period plus an unrestricted supply of sour skim-milk. (iv) As (iii) except that milk kept sweet by the addition of 0·15% formalin shortly after separation, was fed.There were five pens of nine group-fed pigs on each treatment, involving a total of 180 pigs. The pigs were on experiment from about 9 weeks of age to bacon weight. Comprehensive carcass measurements were made on all the pigs, and bacon-tasting tests were made on samples of both green and smoked bacon from a number of the carcasses.There were no significant differences between any of the four treatments in rate of growth or overall efficiency of food utilization.Pigs given formalin-treated milk had a significantly thinner layer of back fat over the loin than those given sour milk. Although the difference was relatively small, it was apparent whether the milk was fed in restricted amounts or ad lib. and was reflected in the commercial grading results. There were no significant differences between treatments in dressing percentage, carcass length, shoulder back fat thickness, belly thickness or size of eye muscle.No taint or off-flavour was detected in samples of green or smoked bacon from pigs fed milk to which 0·15% formalin had been added.The feeding of liquid skim-milk in unrestricted quantities is discussed in relation to the comparative prices of meal and skim-milk.The importance of adding the formalin to the skim-milk as soon as possible after separation, particularly during warm weather, is stressed in order that souring might be delayed for at least 1 week.

171. The effect of commercial sterilization on the nutritive value of milk. IIa. biological value and digestibility of the proteins (nitrogen) of milk

1938 ◽  
Vol 9 (1) ◽  
pp. 6-11 ◽  
Author(s):  
K. M. Henry ◽  
S. K. Kon
Keyword(s):  
Heat Treatment ◽  
Nutritive Value ◽  
Statistical Tests ◽  
Raw Milk ◽  
Biological Value ◽  
True Digestibility ◽  
The Difference ◽  
Sterilized Milk

1. The biological values and true digestibilities of the proteins of raw and commercially sterilized milks from the same bulk have been compared on rats by the method of Mitchell.2. For raw milk a biological value of 84·3 and a true digestibility of 96·4 were obtained, while the figures for sterilized milk were 79·1 and 95·3 respectively.3. Statistical tests showed that the difference in true digestibility was not significant but that the lowering of the biological value by about 6% could be ascribed to the effect of the heat treatment, the odds against chance factors being 625: 1.

scholarly journals The nutritive value of raw and pasteurized milk for calves

1937 ◽  
Vol 37 (2) ◽  
pp. 243-253 ◽  
Author(s):  
G. S. Wilson ◽  
F. C. Minett ◽  
H. F. Carling
Keyword(s):  
Body Weight ◽  
Nutritive Value ◽  
Skeletal Development ◽  
Raw Milk ◽  
Normal Diet ◽  
The Other ◽  
Average Increase ◽  
Milk Diet ◽  
Pasteurized Milk ◽  
Bull Calves

1. An experiment on the feeding of calves is described, which lasted over 2 years, and which was carried out on a healthy Shorthorn herd free from tuberculosis and contagious abortion.2. Alternate animals as they were born were allocated, without any selection whatever, to one or other of two groups. One of these was fed on raw, the other on pasteurized milk. Every animal received its mother's colostrum for 3 days before being put on the milk diet.3. The milk used was taken from the mixed morning's milk of the whole herd. It was divided into two parts, one of which was given raw, the other of which was submitted to low-temperature pasteurization at 145° F. (628° C.) for 30 nun, and subsequently cooled. The animals were fed morning and evening on measured quantities that were in strict relationship to their body weight.4. In addition to the milk, hay was allowed ad lib. Observations showed that practically none was eaten during the first month, after which each animal consumed about 1 lb. a day, rising to 3 lb. by the eighth week.5. With the exception of two weaklings which died after 15 and 23 days respectively from causes apparently unconnected with the nature of their diet, all the animals—twenty-five in the raw and twenty-three in the pasteurized group—throve well, and showed no obvious signs of rickets or anaemia.6. The average increase in weight over the 8-week period for the animals in the raw group was 53·72 lb., and in the pasteurized group 53·86 lb., or 61·18 and 62·94 per cent respectively. This practical identity in weight increase is all the more surprising in view of the fact that the number of bull calves was very much less in the pasteurized than in the raw group.7. The highest individual gain among the bull calves—one of 80 lb.—and the highest individual gain among the heifer calves—one of 63 lb.—both occurred in animals fed on pasteurized milk.8. At no time throughout the experiment was any observer, lay or professional, able to distinguish between the two groups of animals.9. The diet given, though permitting of good skeletal development, was insufficient to fatten the animals. After they had been transferred, however, to a normal diet at the conclusion of their 8 weeks in the experiment, they soon put on weight and within 2 or 3 months were indistinguishable in size or condition from animals that had received a more generous diet from birth.10. There is nothing in these results to suggest that the nutritive value of pasteurized milk for calves is in any way inferior to that of raw milk.

scholarly journals A protective cabinet for the post-mortem examination of infected animals

1959 ◽  
Vol 57 (4) ◽  
pp. 407-409 ◽  
Author(s):  
C. W. Couling ◽  
R. J. W. Rees
Keyword(s):  
Guinea Pigs ◽  
Small Animals ◽  
Post Mortem ◽  
Surrounding Area ◽  
Post Mortem Examination ◽  
Pathogenic Organisms ◽  
Infected Material

A special cabinet was designed to protect the operator, during post-mortem examinations on animals infected with pathogenic organisms, against the risk of enhaling infected material and the surrounding area from becoming contaminated. The cabinet was suitable for small animals including mice and guinea-pigs.

189. The effect of commercial sterilization on the nutritive value of milk. VI. Comparison of the total nutritive value of raw and commercially sterilized milks

1938 ◽  
Vol 9 (2) ◽  
pp. 188-206 ◽  
Author(s):  
K. M. Henry ◽  
E. W. Ikin ◽  
S. K. Kon
Keyword(s):  
Nutritive Value ◽  
Basal Diet ◽  
Raw Milk ◽  
Cane Sugar ◽  
Male Rats ◽  
Limiting Factor ◽  
Protein Energy ◽  
Preceding Experiment ◽  
The Difference ◽  
Sterilized Milk

1. The effect of commercial sterilization on the total nutritive value of milk was studied in five separate experiments.2. In the first experiment raw and sterilized milks supplemented with minerals were given in equal amounts to pairs of litter-mate male rats. No differences were found in the growth performance of the rats, but the rats on sterilized milk consumed it more readily than their mates on raw milk. This difference was statistically significant.3. Rats getting limited but equal amounts of milk in addition to a basal diet which supplied only protein, energy and minerals grew better on raw than on sterilized milk.4. When the basal diet contained in addition 5% brewer's yeast and the milk intakes were the same as in the previous experiment no difference was found between the two groups.5. Rats receiving raw milk to which 15 g. of cane sugar was added per 100 ml. grew better than rats receiving equal quantities of similarly treated sterilized milk.6. Rats given raw milk containing 30 g. per 100 ml. of sugar grew as well as the rats on sterilized milk in the preceding experiment, but rats receiving similarly treated sterilized milk took much less milk than the raw milk rats, grew very poorly and some of them developed symptoms of beri-beri.7. The difference between raw and sterilized milk is discussed and it is concluded that in these experiments on the total nutritive value of milk vitamin B1 was the first limiting factor of sterilized milk.

158. The nutritive value of raw and pasteurized milk for mice

1937 ◽  
Vol 8 (2) ◽  
pp. 203-217 ◽  
Author(s):  
G. S. Wilson ◽  
Irene Maier
Keyword(s):  
Nutritive Value ◽  
Basal Diet ◽  
Raw Milk ◽  
Pasteurized Milk ◽  
White Flour ◽  
The Individual

1. Experiments were carried out during the years 1931–5 in which mice on a basal diet of raw milk and white-flour biscuit were compared with mice on a similar diet except that the raw milk was replaced by commercially holderpasteurized (145–150° F. for 30 min.) milk belonging to the same batch. Each group of mice comprised in aggregate about 1100 animals, which were usually 4–6 weeks old at the commencement of observation. For reasons given on p. 206 no attempt was made to standardize the volume of milk consumed by the individual mice. Except in the preliminary experiments, when the milk was given in excess, the amount supplied was regulated more or less in accordance with the requirements of the average animal.

scholarly journals STUDIES ON THE INTERACTION BETWEEN PHAGOCYTES AND TUBERCLE BACILLI

1957 ◽  
Vol 105 (3) ◽  
pp. 265-277 ◽  
Author(s):  
Hartmann Stähelin ◽  
Manfred L. Karnovsky ◽  
Ann E. Farnham ◽  
Emanuel Suter
Keyword(s):  
Oxygen Uptake ◽  
Guinea Pigs ◽  
Glucose Utilization ◽  
Mononuclear Cells ◽  
Virulent Strain ◽  
Tuberculous Infection ◽  
Polymorphonuclear Leucocytes ◽  
Kidney Slices ◽  
Liver Slices ◽  
The Difference

In continuing studies concerning the interactions between phagocytes and tubercle bacilli the effect of tuberculous infection on respiration and glucose utilization was investigated in guinea pigs. Peritoneal exudates rich in polymorphonuclear leucocytes, derived from guinea pigs infected with tubercle bacilli, had a significantly higher rate of respiration than the same cells from normal animals. The difference between cells from normal and infected animals was greater when the animals were infected with a virulent strain (Vallée) than when infected with an attenuated one (R1Rv or BCG). By the use of glucose labelled with C14 at position 1 or 6, or uniformly labelled glucose, it was established that this difference in oxygen uptake between normal and infected cells was probably not caused by a difference in the pathway of glucose utilization. Similarly, the respiration of liver and kidney slices from normal and infected guinea pigs was compared and it was found that liver slices showed differences similar to those shown by leucocytes, but that the kidney slices did not. The possibility has not been ruled out that the difference in rate of respiration of liver slices due to infection might be caused by tuberculous lesions in the livers of infected animals. The mononuclear cells which invade the liver have a higher rate of oxygen uptake than liver cells. The rate of glucose utilization and the total amount of CO2 produced was also determined in intact guinea pigs. Both functions were found not to differ significantly in normal and infected animals. The rate of production of CO2 from C1 and C6 of glucose was the same in both groups of animals. The ratio of the rate of production of C14O2 from C1 and C6 of glucose by the whole animal was found to be about 1.35. It was found to be much higher with polymorphonuclear leucocytes (C1/C6 = 8 in the absence of serum). During the process of phagocytosis this ratio increased from about 25 to about 130 (in the presence of 2 per cent serum) indicating an increase in the direct oxidative pathway of glucose utilization during stimulated cellular activity.

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