Mycotoxigenic fungi contamination of grains and peanuts from open markets in Kelantan, Malaysia

The warm weather and high relative humidity in Malaysia are ideal for the survival and proliferation of mycotoxigenic fungi leading to a high rate of stored product contamination. This study was conducted to enumerate and characterise the mycotoxigenic fungi associated with commonly consumed food grains in Kelantan, Malaysia. The fungal bioburden and fungal identification from forty-four composite food samples comprising 11 samples each of maize, wheat, rice, and peanuts from open markets in Kelantan, Malaysia, were determined using standard mycological techniques. A total of 115 mould fungal isolates belonging to 12 species were isolated, of which Aspergillus flavus (17.39%), A. versicolor (13.04%), A. felis (12.17%), Neoscytalidium dimidiatum (11.3%), Penicillium cheresanum (11.3%) and P. chrysogenum (8.7%), were predominant. Peanuts were the most contaminated (9.7×105 ± 1.5×105 CFU/g) followed by maize (7.5×105 ± 1.8×106 CFU/g), wheat (1.9×105 ± 2.6×105 CFU/g), and rice (9.9×104 ± 1.5×105 CFU/g). The levels of the mycotoxigenic fungi in peanut, maize, and wheat were above the permissible limit of 102 CFU/g set by the Malaysian Ministry of Health and 102 to 105 CFU/g set by the International Commission for Microbiological Specification for Foods, signifying that they are unsafe for use as food or feed ingredients. Hence, there is a need for more stringent control measures.

Vehicle Traffic as a Source of CO2, CO, HC and Particulate Polycyclic Aromatic Hydrocarbon in the Mingora City, District Swat, KP, Pakistan and Their Effect on Health, Temperature and Tourism

In this work we collected a large number of vehicular emission data of carbon dioxide (CO2), carbon monoxide (CO) and hydrocarbons (HC) from custom paid and non-custom paid vehicles in the Swat district, which are responsible for changing the climate and global warming. Swat valley is facing severe threats and impacts of the climate change as there is a record high increase in the temperature with flash floods and droughts becoming increasingly common. The main cause of the increasing warm weather is vehicle emissions along with the cutting of forest on a large scale in the valley. Hospital records for 2768 children aged 0 to 18 years (697 of whom had two encounters) were obtained for a main city area of two hospitals in Saidu Sharif, Swat. Residential addresses were geocoded. A line source dispersion model was used to estimate individual seasonal exposures to local traffic-generated pollutants (nitrogen oxides, carbon monoxide and hydroxide).

Assessment of Sensitivity and Profitability of an Intravaginal Sensor for Remote Calving Prediction in Dairy Cattle

One critical point of dairy farm management is calving and neonatal first care. Timely calving assistance is associated with the reduction of calf mortality and postpartum uterine disease, and with improved fertility in dairy cattle. This study aimed to evaluate the performance and profitability of an intravaginal sensor for the prediction of stage II of labor in dairy farms, thus allowing proper calving assistance. Seventy-three late-gestating Italian Holstein cows were submitted to the insertion of an intravaginal device, equipped with light and temperature sensors, connected with a Central Unit for the commutation of a radio-signal into a cell phone alert. The remote calving alarm correctly identified the beginning of the expulsive phase of labor in 86.3% of the monitored cows. The mean interval from alarm to complete expulsion of the fetus was 71.56 ± 52.98 min, with a greater range in cows with dystocia (p = 0.012). The sensor worked correctly in both cold and warm weather conditions, and during day- or night-time. The intravaginal probe was well tolerated, as any cow showed lesions to the vaginal mucosa after calving. Using sex-sorted semen in heifers and beef bull semen in cows at their last lactation, the economic estimation performed through PrecisionTree™ software led to an income improvement of 119 € and 123 €/monitored delivery in primiparous and pluriparous cows, respectively. Remote calving alarm devices are key components of “precision farming” management and proven to improve animal welfare, to reduce calf losses and to increase farm incomes.

Mango Leaf Monitoring with Inductive and Capacitive Sensors and Its Comparison with Trunk Dendrometer Measurements

Mango is one of the main fruits grown in Mexico that are exported worldwide, but the trees consume a lot of water, and irrigation scheduling should be implemented to optimize water use. Dendrometers were installed in fruit trees to optimize water usage during 2019 and 2020. A capacitor with Teflon clamps pressurized the leaf, and its dielectric changed with leaf water content. Additionally, Hall sensors were installed in leaves to study the effect of water during mango production. It was found that capacitance tend to be more sensitive than magnetic field monitoring. Higher changes were noted during midday with warm weather. Thresholds from the capacitance and Hall effect sensors can provide signals for irrigation scheduling.

How Prospective International Retired Migrants Use Tourism for Decision Making

Despite the current importance of international retirement migration for both academics and practitioners, the extant literature on the topic is still scarce and mostly focused on short-period migration flows from wealthy and northern countries to cheaper and warm-weather destinations. This article aims at shedding light on the role of tourism in prospective migrants’ decision-making process, considering the framework provided by the push-pull model, which is often used to explain both migration and tourism. A qualitative study was conducted, comprising ten in-depth interviews with 45+ year-old Brazilian citizens who intend to move to Europe after retirement. Results show that tourism is important for prospective migrants to evaluate possible migration destinations, as some of the most relevant migration pull factors (e.g., safety) are easily assessed during tourism experiences. Participants in this study also carefully plan tourism activities prior to their decision to migrate in order to get a more realistic notion of what the destination is like for residents. Overall, this study demonstrates that tourism is particularly important for several stages of migrants’ decision-making process.

Correlation of Royal Jelly composition with swarming tendency in Honey Bees (Apis mellifera): A Review

Honey bees are eusocial insects which respond to warm weather, abundant food source by increasing their population through swarming to ensure the survival of the colony. To maintain a superior colony a queen must have a nutrient-rich diet and high egg production. Royal jelly is a high-quality food which has numerous beneficial properties required for proper growth, development, survival of the queen. Factors like congestion, lack of adequate queen pheromone, abnormal queen pheromone, pathogenic infections, exposure to pesticides influence the queen quality which further promotes non-reproductive swarming behaviour. Worker bees analyse the queen condition to prepare for supersedure or emergency queen rearing. This review paper highlights the influence of royal jelly composition on the queen quality, the impact of queen quality on swarming tendency, correlation between royal jelly composition and swarming tendency.

Responses of Outdoor Housed Dairy Cows to Shade Access during the Prepartum Period under Temperate Summer Conditions

Cows are affected by environmental factors associated with warm weather conditions; however, little is known about the effect of shade access especially during the prepartum period of dairy cows in temperate regions. This study assessed the effect of shade on the behavior (lying, rumination, feeding, and drinking), body fat mobilization, and health status of outdoor-housed dairy cows during the prepartum period under temperate summer conditions. During the 3 weeks prior to calving, 24 multiparous Holstein cows were grouped (4 cows/group) and assigned to either an open corral without shade or with access to shade until calving. We daily measured shade use, lying, rumination, feeding, and drinking behavior. Weekly, prepartum non-esterified fatty acids (NEFA) and postpartum b-hydroxybutyrate (BHB) concentrations were measured. Clinical examination was periodically performed individually until 21 postpartum days. Shade use averaged 45.6, 46.0, and 19.8% during the hottest hours of the day (11–18 h) in weeks 3, 2, and 1 prior to calving, respectively. Shaded cows had higher values for rumination time and feeding time during the morning but spent less time drinking during the warmest hours than unshaded cows. NEFA and BHB concentrations and clinical diseases were similar between both treatments. These findings suggest that under temperate summer conditions the access to an artificial shade is an important resource, observing beneficial effects mainly on behavioral variables.

Significance of Urban Vegetation on Lawns Regarding the Risk of Fire

Urban green infrastructure significantly influences the functioning of a city and the comfort of its residents. Lawns are an essential element of public greenery. They represent a live component, and if they are lacking, of low quality, or neglected, this will cause major problems in the urban environment. The vegetation structure of urban grassy areas changes under the influence of different management methods used for their maintenance. The main goal of this study was to evaluate the species diversity of urban lawns and to determine the influence of this vegetation on factors based on the representation of the species found. Three sites with urban lawns were chosen in a built-up city area where different types of vegetation management were applied: Typical management, in which grassy areas are mowed twice a year; intensive management, in which lawns are mowed several times a year and the biomass is removed; and extensive management, in which lawns are mowed irregularly, once a year at most, and the biomass is left unevenly on the site. Extensive management and unkempt urban grassy areas represent a high risk of fire due to the presence of plant species that produce great amounts of biomass. Combined with dry and warm weather, the dead biomass can lead to outbreaks of fire. Extensive management of urban grassy areas brings some benefits, such as lower maintenance costs and increased biodiversity and bioretention. On the other hand, intensive management reduces the risk of fire and the biodiversity of the plant community. Attention should be paid to the composition of vegetative species and their functions that could threaten the safety of residents, with the risk of fire being one of them. However, the vegetation biomass of grassy areas affected by management practices is only a precondition for the risk of fire because weather and drought occurrence play important roles as well.

An efficient sorghum transformation system using embryogenic calli derived from mature seeds

Significant progress has been made on sorghum transformation in the last decades; however, the transformation process has been constrained by the availability of immature embryos because most of the researchers have utilized immature embryos as favorable explants. Although immature embryos have been proven to be optimal for tissue culture and transformation, isolation of immature embryos is time-consuming, labor-intensive, and limited by warm weather. In this study, we developed an efficient genetic transformation system using mature seeds as explants. The nptII and gus gene, used as the selective marker and report gene respectively, have been co-transformed by particle bombardment. After optimization of tissue culture, the G418 concentration, and transgenic, the average transformation frequency at 13.33% was achieved routinely. The transgenic events and transgene copy numbers were determined by PCR and RT-PCR, respectively. The geneticin selection and GUS staining on T1 seedlings confirmed that the transgenic plants were heritable. Our results demonstrated that the efficient sorghum transformation system has been established using mature seeds as explants. This transformation system will promote sorghum research on genetic engineering and genome editing without seasonal weather conditions restriction and explant resources restriction.