Effect of stage of lactation, stage of pregnancy, milk yield and herd management on seasonal variation in spontaneous lipolysis in bovine milk

1986 ◽  
Vol 53 (4) ◽  
pp. 529-538 ◽  
Author(s):  
Marie-Paule Chazal ◽  
Yves Chilliard

SummaryThe free fatty acid (FFA) levels after milking (initial FFA) and after 22 h storage at 4°C (FFA-22) were determined in milk from 57 cows every 2 weeks for one year. FFA levels which were higher in summer than in winter were explained by later stages of lactation occurring in summer. Initial FFA content increased regularly during lactation. FFA-22 content increased after 12 weeks, and further after 32 weeks of lactation. The first increase was due to stage of lactation. The second increase, which appeared in late lactation, was essentially due to the stage of pregnancy combined with the effect of milk yield. FFA-22 content increased in the second part of pregnancy, chiefly after 24 weeks. A low milk yield (≼ 5 kg) further increased lipolysis, but only after 16 weeks of pregnancy. The effect of stage of pregnancy may have been enhanced by management factors such as feeding conditions. In this investigation, the seasonal FFA variation in bulk tank milk could be explained entirely by physiological factors.

2000 ◽  
Vol 25 ◽  
pp. 165-168
Author(s):  
B.A. Slaghuis ◽  
G.H. Klungel

AbstractThe freezing point of bovine milk is known to vary between narrow limits. However, some variation is possible, because of the osmotic relationship between blood and milk. The extent of variation in freezing points of cow's milk was studied. For one year, freezing points were determined in individual milk samples from a high producing herd. Differences (P<0.05) were found between evening and morning milk yield and freezing points. A ‘lactation curve’ for freezing points was fitted and showed some similarity with milk yield curves. Stage of lactation explained part of the variation of freezing points of cow's milk.


Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1590
Author(s):  
Grażyna Czyżak-Runowska ◽  
Jacek Antoni Wójtowski ◽  
Romualda Danków ◽  
Daniel Stanisławski

The objective of this study was to determine the chemical composition, fatty acid profile, and values of healthy indices of milk from a specialized farm of Polish Coldblood mares of different ages, birth orders, and lactation stages. Milk samples (n = 48) were collected for analysis in weeks 10, 15, and 25 of lactation from mares aged between five and 14 years. The study showed that the stage of lactation has a significant effect on the fatty acid (FA) profile of the milk produced on the farm. The highest concentration of monounsaturated and polyunsaturated FAs was found in milk produced from the 15th week of lactation. The milk was also characterized by low values of atherogenic and thrombogenic indices, which indicate the health benefits of milk with respect to the content of fatty acids and their potential to prevent or cause atherosclerosis and thrombosis. The study also found a significant correlation between the number of foalings (birth order), the fatty acid profile, and atherogenic index of milk produced on the farm. The findings from the study indicate that it is possible to modify the fatty acid profile of bulk tank milk through appropriate management of the age structure of the herd of mares. To confirm this dependence, the study will be continued on a larger group of mares.


1985 ◽  
Vol 52 (2) ◽  
pp. 255-266 ◽  
Author(s):  
Eric C. Needs ◽  
Malcolm Anderson ◽  
Stuart J. Payne ◽  
Elizabeth A. Ridout

SUMMARYThe effect of separating conditions on lipase activity and free fatty acid levels in preheated milk, cream and skim milk was measured on nine occasions during a 12-week period covering the seasonal change from winter feeding to summertime grazing. This change consisted of four periods each representing a different type of forage intake, namely: silage, kale, daytime grazing and 24 h grazing. Milk was separated at 30, 40, 50 and 60°C with preheating times of 10, 25 and 55 s. Results were expressed both as absolute values and in terms of changes relative to the original unheated milk. Lipase activity and free fatty acid concentration were significantly reduced as separation temperature increased but were not influenced by holding time. The loss of activity in cream was progressive so that at 60°C only 40% of the original activity remained. Up to 50°C little change occurred in preheated milk or skim milk activity, while at 60°C 83 and 76% respectively of the original activity remained. The amount of activity calculated to be associated with the fat fraction of the cream also decreased with temperature. Activity varied significantly with date; maximum values were observed during the first 3 weeks of summertime grazing. Relative activity values indicated that the susceptibility of milk lipase to heat inactivation also varied with date. Lipolysis was also significantly affected by date. Cream free fatty acid levels were lower during the period of daytime grazing and were significantly higher than those in preheated milk. The correlation between lipase activity and free fatty acid levels was generally poor, accounting for between 0 and 34% of the variance. Possible reasons for the effect of separating temperature on lipolysis in cream are discussed.


2019 ◽  
Vol 86 (2) ◽  
pp. 226-232 ◽  
Author(s):  
Lars Wiking ◽  
Martin Bjerring ◽  
Mette Marie Løkke ◽  
P. Løvendahl ◽  
T. Kristensen

AbstractFree fatty acid (FFA) concentrations can be elevated in raw milk due to improper handling and management at the dairy farm, and high concentrations of FFA can lead to off flavors in milk. This study aimed to describe how the herd production system, milking system, feeding and technological factors impact on FFA concentrations in bulk tank milk. FFA concentrations in bulk milk samples from 259 organic and 3326 conventional herds were analyzed by FT-IR during one year. The FFA content was significantly lower in bulk milk from organic than conventional herds. This was most evident during the summer half-year when the organic cows graze pasture. Bulk milk from automatic milking systems (AMS) and tie-stalls contained greater concentrations of FFA than any other milking parlor systems. In AMS, high milking frequency was found to be the most significant contributor to elevated FFA content in milk. Moreover, a strong interaction was found between milking interval and production system (organic vs. conventional). The technical factors, pre-cooling, onset time for cooling after milk inlet, contact between milk and agitation also impacted on the FFA concentration, whereas other technical factors including centrifugal pump type, length and height of pumping line and type of AMS manufacturer were found to be without significant effect on FFA. Feeding variables, based on feeding plans and evaluation, only explained a small part of the variation in bulk milk FFA. Overall, this study demonstrated that AMS compared to other milking system contributes significantly to increased FFA concentration in bulk tank milk, and within AMS high milking frequency contributes to increased FFA concentration.


2011 ◽  
Vol 41 (8) ◽  
pp. 1472-1474 ◽  
Author(s):  
Bruno Garcia Botaro ◽  
Cristina Simões Cortinhas ◽  
Lucinéia Mestieri ◽  
Paulo Fernando Machado ◽  
Marcos Veiga dos Santos

The aim of this study was to estimate the concentration of milk true protein (TP) by mid-infrared absorbance method (MIR) in samples from bulk tank of dairy herds, and to determine the correlation between the results of TP of milk determined by Kjeldahl and MIR. Forty nine dairy herds were selected (17 Holstein, 6 Jersey and 26 Girolando) for monthly collections of samples from bulk tanks during the period of one year (284 samples). Fat, lactose, crude protein and total solids were firstly determined by MIR, and then analyzed for total and true protein by Kjeldahl method. The regression equation to estimate TP contents based on MIR crude protein determination was as follows: TP=0.0021+(1.0104xCP), where: TP is the content of true protein, CP is the crude protein content determined by the MIR method, and 0.0155 is the model error term.


2019 ◽  
Vol 9 (1) ◽  
pp. 17-22
Author(s):  
Asma Bouterfa ◽  
Ahmed Bekada ◽  
Abdelkader Homrani ◽  
Abdeltif Amrane ◽  
Akram Zribi ◽  
...  

Stage of lactation (SOL) is a major factor affecting several characteristics of milk such as fatty acids content and composition, protein and main minerals content. These variations may have important quantitative and qualitative consequences on the characteristics of cheese. The aim objective of this study was to analyse the effect of lactation stage on the fat and fatty acids composition of the artisanal camembert type-cheese made from cow’s milk collected in Mostaganem region (Algeria) and provided from three stages of lactation (early, mid and late). In this study and for each stage of lactation, the fat and fatty acid composition of camembert type-cheese were analysedand evaluated. Results showed that the total lipids were related to the stageof lactation (p<0.05), ranging from 14.6% for the 3rd SOL to 23% for the 1stSOL. The fatty acids composition of Camembert-type cheese showed a highpolyunsaturated fatty acids percentage dominated by ω6 and ω3 fatty acidsrepresented by linoleic and α-linolenic acids. Indeed, they recorded maximum values of 2.53% and 0.6% respectively, for the Camembert made withthe milk of the 1st SOL (p<0.05). Concerning monounsaturated fatty acidsclass, oleic acid is found to be the most important fatty acid with a maximumpercentage (26.1%) in Camembert of the 1st SOL (p<0.05). Finally, this studyconcluded that the stage of lactation plays a determining role on the biochemical composition of the camembert type-cheese, particularly on lipidsand essential fatty acids.


1983 ◽  
Vol 50 (3) ◽  
pp. 309-319 ◽  
Author(s):  
Malcolm Anderson ◽  
Eric C. Needs

SummaryThe role of blood serum lipoproteins in stimulating milk lipolysis was investigated by adding blood serum and heparin to the milk of normal cows and cows in which elevated somatic cell counts were induced by intramammary infusion of Escherichia coli endotoxin and Staphylococcus aureus. There was considerable variation between individual milks in the extent to which lipolysis was stimulated. In some milks there was almost no response. For values obtained from 29 cows during the first 6 months of lactation a relationship was observed between free fatty acid (FFA) levels in the untreated and serum-stimulated samples (r = 0·776). FFA values were higher after 18 h at 4 °C in milks from infused quarters than in those from control quarters, but this difference was not entirely due to higher values from the infused quarters at 0 h. Both blood serum and heparin stimulated lipolysis in high cell count milks and control milks. The response to heparin was greater than that to blood serum, but the response to both was highly correlated with FFA levels in the untreated milks. There were no differences in the ability of skim-milks prepared from infused and control quarters to promote lipolysis in recombined milks containing sterilized homogenized milk fat globules as a source of substrate. Incubation of blood serum with 2 μg/ml trypsin for 1 h at 37 °C reduced the ability of the serum to promote lipolysis. The importance of lipase activation by blood serum lipoproteins in relation to milk lipolysis is discussed.


2021 ◽  
Vol 48 (5) ◽  
pp. 100-112
Author(s):  
H. A. Muhammad ◽  
Y. Garba ◽  
D. Ogah ◽  
S.K. Inusa

The aim of the study was to evaluate the effect of milking time, lactation stage and udder traits on milk yield of Red Sokoto and Boer goats at the farm of Centre for Dry-land Agriculture, Bayero University Kano, Nigeria. The first evaluation of milk yield was done at early stage of lactation starting after day seven of kidding to exclude colostrum. Daily collection of milk samples followed in the morning and evening until 10 weeks of lactation. The weeks were divided into 3 stages of lactation as early, mid and late lactation stages. Milk samples were measured using a 100 mL measuring cylinder and later converted into grammes. The external udder measurements were taken in cm after seven days post kidding and fortnightly for the period of lactation. The result obtained shows significant difference (p<0.05) in the total morning milk yield between the goats. The total morning milk yield was higher (2642.00 ± 3.18 g) in the Boer goats than in the Red Sokoto (2371.50± 3.18 g). However, the average daily milk yield, total milk yield and total evening milk yield were not significantly (p>0.05) affected by the breeds of goat. Mean milk yield was significantly (p<0.05) influenced by stage of lactation and time of milking. The milk yield was significantly higher (98.91 g) in the late lactation while the least was obtained in the mid stage of lactation (65.44 g). Moreover, the morning milk yield was significantly higher than the evening milk yield. A significant interaction effect (p<0.05) of lactation stage and milking time was similarly observed. The results indicated that breed significantly (p<0.05) influenced most of the udder traits except UC, UW and TFD. Significantly (p<0.05) higher values of UL, UW, TC, RTL, LTL, CH and TFD were recorded in the Red Sokoto. Furthermore, the Boer goat breed had significantly higher values of NT, UC and DBT.     Le but de l'étude était d'évaluer l'effet de la traite de traite, de la phase de lactation et des traits de la piste sur le rendement du lait de Red Sokoto et des chèvres de Boer à la ferme du Centre d'agriculture à sec, de l'Université Bayero Kano, du Nigéria. La première évaluation du rendement en lait a été effectuée à un stade précoce de l'allaitement à partir de sept jours de blague pour exclure le colostrum. La collecte quotidienne d'échantillons de lait a suivi le matin et le soir jusqu'à 10 semaines de lactation. Les semaines ont été divisées en 3 étapes de la lactation comme des étapes de début et de lactation tardive. Les échantillons de lait ont été mesurés à l'aide d'un cylindre de mesure de 100 ml et convertis ultérieurement en grammes. Les mensurations externes de la mamelle ont été prises en cm après sept jours après la mise bas et tous les quinze jours pendant la période de lactation. Le résultat obtenu présente une différence significative (p <0,05) dans le rendement le lait total du matin entre les chèvres. Le rendement au lait total du matin était plus élevé (2642,00 ± 3,18 g) dans les chèvres Boer que dans le Sokoto rouge (2371.50 ± 3,18 g). Cependant, le rendement quotidien moyen quotidien, le rendement total du lait et le rendement au lait total de la soirée n'étaient pas significativement (p> 0,05) affectés par les races de chèvre. Le rendement en lait moyen était significativement (p <0,05) influencé par la phase de lactation et du temps de traite. Le rendement en lait était significativement plus élevé (98,91 g) à la fin de la lactation, tandis que le moindre a été obtenu au stade moyen de la lactation (65,44 g). De plus, le rendement au lait du matin était nettement supérieur au rendement le lait du soir. Un effet d'interaction significatif (P <0,05) de stade de lactation et de délai de traite a été observé de la même manière. Les résultats ont indiqué que la race de manière significative (p <0,05) a influencé la plupart des traits de pis de la piste, à l'exception des UC, UW et TFD. De manière significative (p <0,05) des valeurs plus élevées d'UL, UW, TC, RTL, LTL, CH et TFD ont été enregistrées dans le Red Sokoto. De plus, la race de chèvre Boer avait des valeurs significativement plus élevées de NT, UC et DBT.


2016 ◽  
Vol 72 (12) ◽  
pp. 760-767
Author(s):  
Renata Klebaniuk ◽  
Grzegorz Kochman ◽  
Edyta Kowalczuk-Vasilev ◽  
Eugeniusz R. Grela ◽  
Maciej Bąkowski ◽  
...  

The aim of the study was to compare the efficiency of two energy preparations used at fixed doses, but for different lengths of time: 1) a loose mixture of propylene glycol and sodium propionate, and 2) a granular formulation of free fatty acids produced from palm oil and palm nut, non-degradable in the rumen.. A three-factorial experiment was carried out on 5 groups of Polish Black and White Holstein-Friesian cows (10 animals each) which were within the transition period. The aim of the experiment was to determine the effect of two energy preparations administered at a constant dose, but over different time periods, on the animals' performance, as well as on their milk yield and composition. The following treatment groups were formed: control group C, which received a standard diet; experimental groups G1 and G2, which received a dietary additive of a glucogenic preparation; and experimental groups F1 and F2, which received a free fatty acid preparation. The diet of the transition cows was supplemented with the full dose of the additives (500g/head/day) for 2 weeks before the expected calving and 3 or 6 weeks of lactation. The present research confirmed the benefits of dietary inclusion of energy supplements for transition cows. Supplementation with both glucogenic and free fatty acid preparations reduced the negative energy balance in the cow’s organism and improved milk production and composition. The milk yield, higher by ca. 10% for the cows fed diets with energy additives (relative to the control treatment), provides evidence of a substantial beneficial effect of supplemental dietary energy on the efficiency of cow milk production. In the present study, the best basic milk composition was obtained in the cows receiving a dietary addition of the glucogenic preparation at a dose of 500g/head/day until the end of the 6th lactation week. The animals from this group also had the highest milk yield evaluated for 305 days. In order to improve the energy balance in high-yielding transition cows, it is recommended that dairy managers apply feed energy additives based on propylene glycol and sodium propionate.


2017 ◽  
Vol 17 (3) ◽  
pp. 873-885 ◽  
Author(s):  
Agnieszka Otwinowska-Mindur ◽  
Ewa Ptak ◽  
Agnieszka Grzesiak

Abstract The objective of this study was to estimate the influence of lactation number, month of milk sampling, lactation stage and herd size on the freezing point of milk of Polish Holstein‑Friesian cows. Data comprised 4,719,787 milk samples from the first seven lactations of 752,770 Polish Holstein- Friesian cows. Milk freezing point (MFP), milk yield, and fat and protein content were analyzed. The mean MFP of milk samples (-0.5326°C) as well as more than 92% of all milk samples did not exceed the quality limit for the freezing point of cows′ raw milk, which, following Polish standards, was taken to be -0.52°C. The freezing point of milk samples, milk yield, fat and protein content were highly significantly affected by all examined factors: month of sampling, successive lactations, stage of lactation, and herd size. MFP was lowest in milk samples taken from January to March, and highest in samples from November to December. MFP increased with lactation number. Mean MFP decreased with time within lactation, except the first stage (5-35 days in milk). Generally, MFP was highest in small herds (up to 9 cows) and lowest in large herds (more than 150 cows).


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