Probiotic potential ofLactobacillusspp. isolated from Brazilian regional ovine cheese

2012 ◽  
Vol 79 (1) ◽  
pp. 119-127 ◽  
Author(s):  
Stela Maris Meister Meira ◽  
Virginia Etges Helfer ◽  
Renata Voltolini Velho ◽  
Fernanda Cortez Lopes ◽  
Adriano Brandelli
Keyword(s):  
Bile Salts ◽  
Acid Tolerance ◽  
Acid Oxidation ◽  
16S Rdna Sequencing ◽  
Galactosidase Activity ◽  
Dpph Radical ◽  
Probiotic Potential ◽  
Rdna Sequencing ◽  
Relative Tolerance ◽  
Ovine Cheese

TwelveLactobacillusisolates from Brazilian starter-free ovine cheeses were evaluated for their probiotic potential. The strains were identified by 16S rDNA sequencing asLactobacillus plantarum(7),Lb. brevis(2),Lb. casei(2) andLb. parabuchneri(1). All strains showed variable resistance to gastric juices and relative tolerance to pancreatin and bile salts. Only five strains ofLb. plantarumcould not deconjugate the sodium salt of taurodeoxycholic acid. Autoaggregation ability after 24 h was above 50% and hydrophobicity was higher than 60% for most strains. All lactobacilli could inhibit linolenic acid oxidation, exceptLb. parabuchneristrain, whereas none of them could scavenge DPPH radical. β-Galactosidase activity ranged from 47·7 to 2503 Miller units. Inhibition of food pathogensListeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Escherichia coliandSalmonella typhimuriumwas demonstrated and the production of organic acids could be associated with this effect. TheLactobacillusstrains from Brazilian regional ovine cheese showed interesting functional characteristics, mainly the strainsLb. brevisSM-B andLb. plantarumSM-I. Both presented high acid tolerance. In addition,Lb. brevisSM-B also displayed remarkable antioxidant activity andLb. plantarumSM-I was the highest β-galactosidase producer, exhibited high autoaggregation and hydrophobicity properties.

Metabolite fingerprinting of novel Streptomyces UK-238 from the Himalayan forest

2020 ◽  
Vol 16 ◽  
Author(s):  
Nidhi Srivastava ◽  
Indira P. Sarethy
Keyword(s):  
Ethyl Acetate ◽  
16S Rdna ◽  
Retention Index ◽  
Ethyl Acetate Extract ◽  
Similarity Index ◽  
Antimicrobial Drug ◽  
16S Rdna Sequencing ◽  
Metabolite Fingerprinting ◽  
Streptomyces Sp ◽  
Rdna Sequencing

Aims: Characterization of antimicrobial metabolites of novel Streptomyces sp. UK-238. Background: Novel antimicrobial drug discovery is urgently needed due to emerging multi antimicrobial drug resistance among pathogens. Since many years, natural products have provided the basic skeletons for many therapeutic compounds including antibiotics. Bioprospection of un/under explored habitats and focussing on selective isolation of actinobacteria as major reservoir of bio and chemodiversity has yielded good results. Objective: The main objectives of the study were the identification of UK-238 by 16S rDNA sequencing and antimicrobial metabolite fingerprinting of culture extracts. Method: In the present study, a promising isolate, UK-238, has been screened for antimicrobial activity and metabolite fingerprinting from the Himalayan Thano Reserve forest. It was identified by 16S rDNA sequencing. Ethyl acetate extract was partially purified by column chromatography. The pooled active fractions were fingerprinted by GC-MS and compounds were tentatively identified by collated data analysis based on Similarity Index, observed Retention Index from Databases and calculated Retention Index. Results: UK-238 was identified as Streptomyces sp. with 98.4% similarity to S. niveiscabiei. It exhibited broad-spectrum antibacterial and antifungal activity. GC-MS analysis of active fractions of ethyl acetate extract showed the presence of eighteen novel antimicrobial compounds belonging to four major categories- alcohols, alkaloid, esters and peptide. Conclusion: The study confirms that bioprospection of underexplored habitats can elaborate novel bio and chemodiversity.

PSVII-9 Influence of lactose and milk oligosaccharides in whey permeate on jejunal mucosa-associated microbiota in nursery pigs during 7 to 11 kg BW

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 407-407
Author(s):  
Ki Beom Jang ◽  
Sung Woo Kim
Keyword(s):  
Alpha Diversity ◽  
Jejunal Mucosa ◽  
16S Rdna Sequencing ◽  
Sequencing Data ◽  
Milk Oligosaccharides ◽  
Whey Permeate ◽  
Nursery Pigs ◽  
Rdna Sequencing ◽  
Acid Producing Bacteria ◽  
Diversity Estimates

Abstract This study aimed to evaluate supplemental effects of milk carbohydrates in whey permeate on jejunal mucosa-associated microbiota in nursery pigs during 7 to 11 kg BW. A total of 720 pigs at 7.5 kg BW were allotted to 6 treatments (6 pens/treatment and 20 pigs/pen). Treatments were 6 levels of whey permeate supplementation (0, 3.75, 7.50, 11.25, 15.00, and 18.75%) and fed to pigs for 11 d. On d 11, 36 pigs representing median BW of each pen were euthanized to collect the jejunal mucosa to evaluate microbiota in the jejunum by 16S rDNA sequencing. Data were analyzed using contrasts in MIXED procedure of SAS. Whey permeate contained 76.3% lactose and 0.4% milk oligosaccharides. Increasing whey permeate supplementation from 0 to 18.75% did not affect the alpha-diversity estimates of microbiota. Whey permeate supplementation tended to decrease (P = 0.073, 1.59 to 1.22) Firmicutes:Bacteroidetes compared with no addition of whey permeate. Increasing whey permeate supplementation tended to linearly increase Bifidobacteriaceae (P = 0.089, 0.73 to 1.11), decrease Enterobacteriaceae (P = 0.091, 1.04 to 0.52), decrease Stretococcaceae (P = 0.094, 1.50 to 0.71), and caused quadratic changes (P < 0.05) on Lactobacillaceae (maximum: 9.14% at 12.91% whey permeate). Increasing whey permeate supplementation caused a quadratic change (P < 0.05) on Lactobacillus_Salivarius (maximum: 0.92% at 7.35% whey permeate) and tended to cause quadratic changes on Lactobacillus_Rogosae (P = 0.083; maximum: 0.53% at 8.45% whey permeate) and Lactobacillus_Mucosae (P = 0.092; maximum: 0.70% at 6.98% whey permeate). In conclusion, supplementation of whey permeate as sources of lactose and milk oligosaccharides at a range from 7 to 13% seems to be beneficial to nursery pigs by increasing the abundance of lactic acid-producing bacteria in the jejunal mucosa.

scholarly journals 2-Way k-Means as a Model for Microbiome Samples

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Weston J. Jackson ◽  
Ipsita Agarwal ◽  
Itsik Pe’er
Keyword(s):  
Unsupervised Learning ◽  
16S Rdna ◽  
Mixture Model ◽  
Vaginal Microbiome ◽  
16S Rdna Sequencing ◽  
Sequencing Data ◽  
Cluster Assignment ◽  
Rdna Sequencing

Motivation. Microbiome sequencing allows defining clusters of samples with shared composition. However, this paradigm poorly accounts for samples whose composition is a mixture of cluster-characterizing ones and which therefore lie in between them in the cluster space. This paper addresses unsupervised learning of 2-way clusters. It defines a mixture model that allows 2-way cluster assignment and describes a variant of generalized k-means for learning such a model. We demonstrate applicability to microbial 16S rDNA sequencing data from the Human Vaginal Microbiome Project.

scholarly journals Characterization and antimicrobial activity of vaginal lactobacillus isolate

2011 ◽  
Vol 63 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Gordana Zavisic ◽  
Zeljka Radulovic ◽  
Valentina Vranic ◽  
Jelena Begovic ◽  
L. Topisirovic ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Survival Rate ◽  
Bile Salts ◽  
Antimicrobial Effect ◽  
Low Ph ◽  
Probiotic Potential ◽  
Pathogenic Escherichia Coli

The aim of this study was to investigate the probiotic potential of bacteriocin-producing lactobacilli strain Lactobacillus plantarum G2 isolated from the vaginal mucus of healthy women. The antimicrobial effect of G2 was confirmed in the mixed culture with pathogenic Escherichia coli, Staphylococcus aureus, Salmonella abony and Pseudomonas aeruginosa, while bacteriocine activity was detected against S. aureus and S. abony only. The strain showed an excellent survival rate in low pH and in the presence of bile salts. The percentage of adhered cells of L. plantarum G2 to hexadecane was 63.85?2.0 indicating the intermediate hydrophobicity.

Analysis of the bacterial floral structure and diversity of Xuanwei ham by 16S rDNA sequencing

2020 ◽  
Vol 40 (4) ◽  
Author(s):  
Luying Shan ◽  
Yinjiao Li ◽  
Shi Zheng ◽  
Yuanmiao Wei ◽  
Ying Shang
Keyword(s):  
16S Rdna ◽  
16S Rdna Sequencing ◽  
Floral Structure ◽  
Rdna Sequencing

scholarly journals ISOLATION, PURIFICATION, AND CHARACTERIZATION OF LIPASE FROM BACILLUS SP. FROM KITCHEN GREASE

2018 ◽  
Vol 11 (6) ◽  
pp. 224
Author(s):  
Jaiganesh R ◽  
Jaganathan Mk
Keyword(s):  
16S Rdna ◽  
Solvent Tolerance ◽  
16S Rdna Sequencing ◽  
Bacillus Sp ◽  
Purification And Characterization ◽  
Lipase Enzyme ◽  
Sequencing Method ◽  
Rdna Sequencing ◽  
Solvent Tolerant

Objective: The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. from kitchen grease for a variety of applications including organic synthetic reactions and preparation of enantiomerically pure pharmaceuticals.Methods: Lipase producing isolates were screened from kitchen grease on a selective medium rhodamine B olive oil agar, and tributyrin agar was used to screen the lipase and esterase producing an organism, respectively. The isolate identified using 16S rDNA sequencing method and enzyme activity was quantitatively assayed. Lipase production was characterized in different conditions.Results: The isolate showed highest lipase activity was which later was identified as Bacillus sp. using 16S rDNA sequencing method. The lipase was purified using ammonium sulfate precipitation. The isolate showed excellent tolerance to methanol, ethanol, acetonitrile, and moderate tolerance to butanol. The increased biomass concentration, maximum production, and activity were achieved at 37°C in 24 h incubation, then gradual reduction in production was observed. The maximum activity of lipase enzyme was obtained at pH between 6 and 9.Conclusion: The isolate produce solvent tolerance lipase enzyme and it can be a promising candidate of solvent tolerance lipase enzyme for variety of industrial applications.

Gut microbiota community adaption during young children fecal microbiota transplantation by 16s rDNA sequencing

2016 ◽  
Vol 206 ◽  
pp. 66-72 ◽  
Author(s):  
Jian-Lei Gu ◽  
Yi-Zhong Wang ◽  
Shi-Yi Liu ◽  
Guang-Jun Yu ◽  
Ting Zhang ◽  
...  
Keyword(s):  
Young Children ◽  
Gut Microbiota ◽  
16S Rdna ◽  
Fecal Microbiota Transplantation ◽  
Fecal Microbiota ◽  
16S Rdna Sequencing ◽  
Rdna Sequencing

scholarly journals Determination of uterine bacterial community in postpartum dairy cows with metritis based on 16S rDNA sequencing

2020 ◽  
Vol 10 ◽  
pp. 100102
Author(s):  
Hao Chen ◽  
Kaiqiang Fu ◽  
Binbin Pang ◽  
Jifang Wang ◽  
Huatao Li ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Dairy Cows ◽  
16S Rdna ◽  
16S Rdna Sequencing ◽  
Rdna Sequencing

scholarly journals Detection of Arcobacter spp. in Mytilus galloprovincialis samples collected from Apulia region

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Elisabetta Bonerba ◽  
Anna Mottola ◽  
Antonio Parisi ◽  
Angela Di Pinto ◽  
Andrea Serraino ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Disease Outbreak ◽  
16S Rdna Sequencing ◽  
Emerging Pathogen ◽  
Chain Reaction ◽  
Selective Enrichment ◽  
Apulia Region ◽  
Rdna Sequencing ◽  
Arcobacter Butzleri ◽  
Polymerase Chain

The aim of the study was to evaluate the occurrence of <em>Arcobacter</em> spp. in 20 samples of <em>Mytilus galloprovincialis</em> purchased at fish markets in Apulia region. The detection of <em>Arcobacter</em> spp. was performed, after selective enrichment, on modified charcoal cefoperazone deoxycholate (mCCD) agar supplemented with Cefoperazone, Amphotericin B and Teicoplanin (CAT). In 6 out of the 20 tested samples the presence of <em>Arcobacter</em> spp. was found and confirmed by genus-based polymerase chain reaction. All the isolates were identified as belonging to the species<em> Arcobacter butzleri</em> using 16S rDNA sequencing and BLAST online. The results represent the first report in Italy of <em>A. butzleri</em> detection in marketed <em>Mytilus galloprovincialis</em>. The survey underlines the epidemiological importance of <em>A. butzleri</em> as an emerging pathogen, and highlights that mussels should be considered as a potential cause of foodborne disease outbreak.

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