Domorganus macronephriticus n.g.,n.sp., a new cylindrolaimid free-living soil nematode

1946 ◽  
Vol 21 (4) ◽  
pp. 175-180 ◽  
Author(s):  
T. Goodey

The nematodes described and figured in the following pages are specimens mounted in glycerine which were obtained in 1931 (5 males and 4 females) and in 1932 (one female) from pasture soil at Winches Farm, St. Albans. Unfortunately they were not closely examined in the living condition and it is therefore impossible to give any information about their appearance and movements when alive. The nine specimens obtained at the earlier date were passed on to the writer by Dr. D. O. Morgan (who at that time was on the staff of this Institute) and had been found by him whilst examining earthworms in connection with investigations on “gapes” in chickens and young starlings upon which he was engaged at that time, i.e. March, 1931. These worms, after being killed and fixed in weak formalin and processed through dilute glycerine, were finally mounted in glycerine but no detailed study of them was made until recently when, in the course of re-arranging the slide preparations of free-living nematodes, made during the course of several years, they called for identification. The single female worm obtained in April, 1932, was found on a slide along with specimens of Anguillulina agricola, Anguillulina dubia and a female of Teratocephalus terrestris, all of which had been obtained in a Baermann funnel extraction of a piece of turf taken from one of the meadows at Winches Farm.

2003 ◽  
Vol 66 (9) ◽  
pp. 1543-1549 ◽  
Author(s):  
GARY L. ANDERSON ◽  
KRISHAUN N. CALDWELL ◽  
LARRY R. BEUCHAT ◽  
PHILLIP L. WILLIAMS

Free-living nematodes may harbor, protect, and disperse bacteria, including those ingested and passed in viable form in feces. These nematodes are potential vectors for human pathogens and may play a role in foodborne diseases associated with fruits and vegetables eaten raw. In this study, we evaluated the associations between a free-living soil nematode, Caenorhabditis elegans, and Escherichia coli, an avirulent strain of Salmonella Typhimurium, Listeria welshimeri, and Bacillus cereus. On an agar medium, young adult worms quickly moved toward colonies of all four bacteria; over 90% of 3-day-old adult worms entered colonies within 16 min after inoculation. After 48 h, worms moved in and out of colonies of L. welshimeri and B. cereus but remained associated with E. coli and Salmonella Typhimurium colonies for at least 96 h. Young adult worms fed on cells of the four bacteria suspended in K medium. Worms survived and reproduced with the use of nutrients derived from all test bacteria, as determined for eggs laid by second-generation worms after culturing for 96 h. Development was slightly slower for worms fed gram-positive bacteria than for worms fed gram-negative bacteria. Worms that fed for 24 h on bacterial lawns formed on tryptic soy agar dispersed bacteria over a 3-h period when they were transferred to a bacteria-free agar surface. The results of this study suggest that C. elegans and perhaps other free-living nematodes are potential vectors for both gram-positive and gram-negative bacteria, including foodborne pathogens in soil.


2008 ◽  
Vol 422 (1) ◽  
pp. 321-323
Author(s):  
T. B. Kalinnikova ◽  
A. Kh. Timoshenko ◽  
D. Yu. Galaktionova ◽  
T. M. Gainutdinov ◽  
M. Kh. Gainutdinov

Parasitology ◽  
2008 ◽  
Vol 136 (2) ◽  
pp. 193-201 ◽  
Author(s):  
P. NEJSUM ◽  
A. ROEPSTORFF ◽  
T. J. C. ANDERSON ◽  
C. JØRGENSEN ◽  
M. FREDHOLM ◽  
...  

SUMMARYThe genotypes of both host and parasite may influence the outcome of parasitic infections, but few attempts have been made to quantify the effect of parasite genotype on macroparasite infections of socio-economic importance. We examined variation in particular traits during the infection in pigs with the parasitic nematodeAscaris suum. We infected 26 pigs with mixtures of equal proportions of embryonated eggs from 4 single female worms each with a unique mtDNA haplotype – the eggs from each female worm were a mixture of siblings and half-siblings. Pigs were necropsied on days 14, 17 and 28 following inoculation, which corresponded to time-points before, during and after the main immune responses against the nematode. A total of approximately 11 000 worms were recovered at necropsy. The location in the small intestine was recorded for all worms and the length and mtDNA haplotype were determined for about 4200 individual worms. There were significant differences in the distribution and abundance of the 4 individual haplotypes among individual pigs demonstrating strong interactions between parasite and host. We found significant differences in the abundance and position in the small intestine as well as the size of worms among haplotypes. We conclude that both parasite and host effects as well as the interplay between them play important roles in determining the characteristics and outcome of infection.


2011 ◽  
Vol 48 (2) ◽  
pp. 124-136 ◽  
Author(s):  
V. Čermák ◽  
V. Gaar ◽  
L. Háněl ◽  
K. Široká

AbstractComposition and vertical distribution of soil nematode communities within soil profile were investigated in eight hop gardens in Czech Republic. In total, the presence of 78 nematode genera was confirmed. Genus Drilocephalobus (Coomans & Coomans, 1990) is new for fauna of the Czech Republic. The highest abundance of soil nematodes was found at a depth of 0–10 cm and declined with increasing depth of soil profile. The most dominant genus was Bitylenchus, followed by genera Acrobeloides, Ditylenchus, Chiloplacus and Cervidelus. Ten genera of plant parasitic nematodes were recorded: Bitylenchus (with prevalence of B. dubius), Helicotylenchus, Heterodera (with absolute prevalence of H. humuli), Geocenamus, Longidorella, Longidorus (only L. elongatus), Merlinius (with prevalence of M. brevidens), Paratylenchus and Pratylenchus. Low population densities of predators and omnivores, low values of the community indices (MI, ΣMI, SI, and CI), and high values of NCR, EI, and PPI/MI ratio indicated disturbed nematode communities in hop gardens and bacteria-dominated decomposition pathways in the soil food web.


Nematology ◽  
2011 ◽  
Vol 13 (5) ◽  
pp. 553-559 ◽  
Author(s):  
Robert McSorley

AbstractFree-living nematodes in soil ecosystems are vital in the decomposition of organic matter and recycling of nutrients. The effects of various types of disturbances on nematode assemblages were examined in several experiments on a single soil: a short-term detrimental disturbance from solarisation, a short-term beneficial disturbance from amendment addition, and a long-term detrimental disturbance from bare ground without plant cover. Comparison of solarised and non-solarised field plots revealed lower numbers of fungivores in solarised plots. As a result, indices involving ratios of fungivores to bacterivores and fungivores to total free-living nematodes were decreased as well. Addition of an amendment increased numbers of bacterivores. This change was also reflected in calculated indices that expressed trophic groups as percentages of the total free-living nematodes. A severely disturbed site with bare ground that was without plant cover for more than 5 years was compared with an adjacent site maintained in grass. No plant-parasitic nematodes were recovered from the bare ground site, which contained lower numbers of nematodes in all trophic groups than the grass site. However, the structure of the free-living nematode assemblages in both sites was similar, as indicated by the proportions of various trophic groups and by ratios of fungivores and bacterivores. The free-living nematode assemblage in a site with a perceived severe long-term disturbance maintained a trophic structure similar to a site with perennial plant cover.


2019 ◽  
Vol 22 (3) ◽  
pp. 337-343
Author(s):  
M. O. Pulido-Medellín ◽  
J. C. Giraldo-Forero ◽  
G. I. Chavarro-Tulcán

Giardia spp. is a zoonotic protozoan that causes acute gastroenteritis in humans and other animal species across the world. The aim of this study was to determine the prevalence of Giardia spp. in free living and owned dogs from Tunja - Colombia by Ritchie concentration method and to identify the risk factors (living condition, age, sex, stool consistency, body condition and hair condition) associated with infection. To address the objective a cross-sectional study was carried out. The study population consisted of 200 dogs. Faecal samples were collected from 100 stray dogs and 100 owned dogs using a convenient sampling method. Risk factors were analysed by logistic regression analysis. Risk was expressed as an odds ratio (OR) with 95% confidence interval. Descriptive epidemiology and risk factors were performed using EpiInfo software. A P value of <0.05 was statistically significant. The overall prevalence of Giardia spp. was 39%; cysts were detected by microscopy in 38 of 100 stray dogs and 40 of 100 owned dogs. It was concluded that the prevalence of Giardia spp. in Tunja city was high, with no distinction between stray and owned dogs. We also found that the analysed variables (sex, age, hair condition, body condition and stool consistency) did not constitute risk factors for infection with Giardia spp.


2018 ◽  
Author(s):  
Seaw Jia Liew ◽  
Alex Wilhelm Gorny ◽  
Chuen Seng Tan ◽  
Falk Muller-Riemenschneider

BACKGROUND mHealth approaches are gaining popularity to address low levels of physical activity (PA). OBJECTIVE This study aimed to: (1) develop an mHealth suite, combining PA wearables and an interactive smartphone application (App) supported by a web-based data management system, (2) determine the validity of the wearables in measuring steps per day and floor-count, and (3) assess feasibility and effects of a 6-week team challenge intervention. METHODS Staff and students from a public university were recruited between 2015 and 2016. In Phase 1, every participant was requested to wear a Fitbit tracker (Charge™ or Charge HR™) and an ActiGraph™ for 7 days to measure daily step counts under free-living condition. They were also asked to climb 4 bouts of floors in an indoor stairswell to measure floor-counts. Steps per day and floor-counts estimated by Fitbit™ were compared against ActiGraph and direct observation, respectively. In Phase 2, participants were allocated to control or intervention group and received a Fitbit tracker synced to the Fitbit App. Further, the intervention participants were randomized to 4 teams and used the developed mHealth suite. Teams competed in 6 weekly (Monday - Friday) real-time challenges. A valid day was defined as having accumulated ≥1,500 steps per day. Outcomes were: (i) adherence to wearing Fitbit (i.e. number of days in which all participants in each group was classified as valid users aggregated across entire study period), (ii) mean proportion of valid participants over the study period, and (iii) the effects of intervention on steps and floor-counts determined using multiple linear regressions models and generalized estimating equations (GEE) for longitudinal data analysis. RESULTS In Phase one, 32/40 (steps) and 40/40 (floors) participants provided valid data. The Fitbit trackers demonstrated a high to very high correlation (steps: Spearman Rho=0.89, P < .001, floors: Spearman Rho=0.98, P < .001), respectively. The trackers over-estimated step-counts in free-living condition (median absolute error: 17%) but accurately estimated floor-counts. In Phase two, 20 participants each were allocated to intervention and control. 24 completers (i.e. provided complete covariates and valid PA data) were included in the analyses. Multiple linear regressions revealed 15.9% higher average steps/day (95% CI: -8.9, 47.6, P= .21) and 39.4% higher average floors/day (95% CI: 2.4, 89.7, P= .04) in the intervention group during the final two intervention weeks. GEE results indicated no significant interaction effects between groups and intervention week for weekly step counts, whereas a significant effect (P< .001) was observed for weekly floor counts. CONCLUSIONS The consumer wearables integrated in our mHealth suite provided acceptable validity in estimating stepping and stairs climbing activities. The mHealth suite was feasible for implementing real-time team-challenge interventions. Compared to the controls, the intervention participants performed more stairs climbing which could be introduced as an additional PA promotion target in the context of mHealth strategies. Methodologically rigorous studies with larger sample-size and long-term follow-up are warranted to strengthen the evidence for the proposed mHealth strategy.


2019 ◽  
Vol 40 (1-2) ◽  
pp. 1-6
Author(s):  
Tancredo Souza ◽  
Lucas Sombra Barbosa ◽  
Edjane Oliveira de Lucena ◽  
Luan Nunes de Melo ◽  
Guilherme Silva de Podestá ◽  
...  

The effects of a non-legume cover crop on total soil nematode abundance and soil groups of nematodes were investigated in a long-term field study. We compared total abundance of soil nematode and the abundance of both free-living soil nematode and root-feeding soil nematode under three different treatments (Pennisetum glaucum (L.) R. Br., native weeds and Caatinga moist-forest enclaves treatments) in a long-term field study cultivated on a Regosol. We found the highest abundance of free-living soil nematode in the Caatinga moist-forest enclaves treatment, whereas the highest abundance of root-feeding nematode was found in the native weeds treatment. We did not find any difference among P. glaucum and native weeds treatments for total abundance of soil nematode. Our findings suggest that the introduction of P. glaucum in sandy soil of Brazilian semiarid can increase the abundance of free-living nematode, but in other hand this cover crop did not reduce the abundance of root-feeding nematode. Our results also highlight the importance of considering the identification of soil nematode groups as indicator of soil quality and impacts of soil management.


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