Respiration and carbohydrate energy metabolism of the lung-dwelling parasite Rhabdias bufonis (Nematoda: Rhabdiasoidea)

Parasitology ◽  
1978 ◽  
Vol 76 (1) ◽  
pp. 21-27 ◽  
Author(s):  
A. O. Anya ◽  
G. M. Umezurike
Keyword(s):  
Lactate Dehydrogenase ◽  
Energy Metabolism ◽  
Malate Dehydrogenase ◽  
Malic Enzyme ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Weight Basis ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Alternative Pathways

SummaryAn investigation of the carbohydrate energy metabolism of Rhabdias bufonis, the lung-dwelling nematode parasite of the African toad, Bufo regularis, indicates that the nematode stores very little glycogen (0·137 ± 0·003% on a fresh weight basis) but does utilize oxygen in vitro. The intracellular distribution and high levels of activity observed for the enzymes phosphoenolpyruvate carboxykinase, pyruvate kinase, lactate dehydrogenase, malate dehydrogenase, malic enzyme and fumarate reductase suggest two alternative pathways of carbohydrate energy metabolism.

In vitro variation of glycogen content in three sheep nematodes

Parasitology ◽  
1979 ◽  
Vol 78 (3) ◽  
pp. 355-359 ◽  
Author(s):  
G. Premvati ◽  
Ashok K. Chopra
Keyword(s):  
Haemonchus Contortus ◽  
Glycogen Content ◽  
Physiological Saline ◽  
Weight Basis ◽  
Rate Of Change ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Aerobic Conditions ◽  
Oesophagostomum Columbianum

SUMMARYIn vitro variation of glycogen content under aerobic conditions was measured on fresh weight basis in 3 sheep nematodes inhabiting different niches; Haemonchus contortus, Oesophagostomum columbianum and Trichuris ovis. The parasites were separated into species and then sexes and starved for varying periods of time up to 24 h in glucose-free physiological saline. The differences between females and males and among the species with respect to glycogen content and its rate of change with time are discussed.

Renal enzymes during experimental diabetes mellitus in the rat. Role of insulin, carbohydrate metabolism, and ketoacidosis

1984 ◽  
Vol 62 (1) ◽  
pp. 70-75 ◽  
Author(s):  
Guy Lemieux ◽  
Manuel Rengel Aranda ◽  
Pierrette Fournel ◽  
Christiane Lemieux
Keyword(s):  
Lactate Dehydrogenase ◽  
Glutamine Synthetase ◽  
Glutamate Dehydrogenase ◽  
Malate Dehydrogenase ◽  
Ammonium Chloride ◽  
Malic Enzyme ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Diabetic Rats ◽  
Additional Role

The activities of various ammoniagcnic, gluconeogenic, and glycolytic enzymes were measured in the renal cortex and also in the liver of rats made diabetic with streptozotocin. Five groups of animals were studied: normal, normoglycemic diabetic (insulin therapy), hyperglycemic, ketoacidotic, and ammonium chloride treated rats. Glutaminase I, glutamate dehydrogenase, glutamine synthetase, phosphoenolpyruvate carboxykinase (PEPCK), hexokinase, phosphofructokinase, fructose-1,6-diphos-phatase, malate dehydrogenase, malic enzyme, and lactate dehydrogenase were measured. Renal glutaminase I activity rose during ketoacidosis and ammonium chloride acidosis. Glutamate dehydrogenase in the kidney rose only in ammonium chloride treated animals. Glutamine synthetase showed no particular variation. PEPCK rose in diabetic hyperglycemic animals and more so during ketoacidosis and ammonium chloride acidosis. It also rose in the liver of the diabetic animals. Hexokinase activity in the kidney rose in diabetic insulin-treated normoglycemic rats and also during ketoacidosis. The same pattern was observed in the liver of these diabetic rats. Renal and hepatic phosphofructokinase activities were elevated in all groups of experimental animals. Fructose-1,6-diphosphatase and malate dehydrogenase did not vary significantly in the kidney and the liver. Malic enzyme was lower in the kidney and liver of the hyperglycemic diabetic animals and also in the liver of the ketoacidotic rats. Lactate dehydrogenase fell slightly in the liver of diabetic hyperglycemic and NH4Cl acidotic animals. The present study indicates that glutaminase I is associated with the first step of increased renal ammoniagenesis during ketoacidosis. PEPCK activity is influenced both by hyperglycemia and ketoacidosis, acidosis playing an additional role. Insulin appears to prevent renal gluconeogenesis and to favour glycolysis. The latter would seem to remain operative in hyperglycemic and ketoacidotic diabetic animals.

scholarly journals Hormonal Effects on the Biosynthesis of Tobacco RuBPCase in vitro

1981 ◽  
Vol 11 (1) ◽  
Author(s):  
S.D. Kung ◽  
P.R. Rhodes ◽  
G.W. Schaeffer ◽  
T.C. Tso
Keyword(s):  
Weight Basis ◽  
Wild Type ◽  
Fresh Weight ◽  
Drastic Reduction ◽  
Fresh Weight Basis ◽  
Albino Plants ◽  
Murashige Skoog ◽  
John Williams ◽  
Indole 3 Acetic Acid

Abstract1. The three genotypes, Su/Su, Su/su, su/su, of John Williams Broadleaf tobacco (N. tabacum) grew equally well on Murashige-Skoog medium. On a fresh weight basis, the heterozygous (Su/su) yellow mutant and the homozygous (su/su) wild type plants contain similar amounts of RuBPCase and Fraction II protein in roughly equal proportions, but with a somewhat reduced amount of 70S and 80S free ribosomes. The homozygous albino (Su/Su) plants contain appreciable amounts of RuBPCase and free 70S ribosomes, although the chlorophyll levels are extremely low. In the case of the yellow and albino forms, there appears to be no correlation between RuBPCase and chlorophyll content.`2. When equal concentrations (1 mg/l) of kinetin and indole-3-acetic acid (IAA), or kinetin alone, were added to the medium, a drastic reduction of RuBPCase and disappearance of free 70S ribosomes were observed in the yellow mutant and wild type plants, whereas the effect on the albino plants was not significant.

Yield Performance of Calocybe indica on Different Agricultural Subatrate

2016 ◽  
Vol 2 (3) ◽  
pp. 105
Author(s):  
Pinkal Patel ◽  
Ratna Trivedi
Keyword(s):  
Wheat Straw ◽  
Maximum Yield ◽  
Weight Basis ◽  
Biological Efficiency ◽  
Fresh Weight ◽  
Paddy Straw ◽  
Yield Performance ◽  
Fresh Weight Basis ◽  
Temperature And Humidity ◽  
Calocybe Indica

The Milky mushroom, Calocybe Indica was cultivated on different agricultural substrate, paddy straw, wheat straw, sugarcane trace and mango dry leaves. The spawning was done by sterilization of all the four substrate. The bags were kept in mushroom growing room with the maintenance of temperature and humidity 30̊ c-35̊ c and 70-80 % respectively. The minimum days requires for completion of spawn run (18.4 days), primordial formation (25.2 days) and days for first harvest (32.4 days) was first observed on cultivation with Paddy straw.  The maximum yield on fresh weight basis and biological efficiency (134.86 %) was also found to be as the same treatment with the Paddy straw as a substrate. The biological efficiency of wheat straw was at par with Sugarcane trace as substrate which was 85.07 % and 85.02 % respectively.

Nitrogen metabolism of Picea glauca. II. Diurnal changes of free amino acids, amides, and guanidino compounds in roots, buds, and leaves during the onset of dormancy of white spruce saplings

1968 ◽  
Vol 46 (7) ◽  
pp. 921-928 ◽  
Author(s):  
D. J. Durzan
Keyword(s):  
Amino Acids ◽  
Picea Glauca ◽  
Weight Basis ◽  
Aminobutyric Acid ◽  
Soluble Nitrogen ◽  
Diurnal Changes ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Plant Parts ◽  
Guanidino Compounds

In late August during the onset of dormancy in spruce, seasonal levels of soluble nitrogen, rich in arginine, were high. On a fresh weight basis, diurnal levels of total soluble nitrogen and most component amino acids in roots, buds, and leaves showed maxima, one at sunrise and another in the afternoon or near sunset.Arginine and glutamine in the different plant parts contributed 44 to 83% to the alcohol-soluble nitrogen. In buds and leaves, percentage of arginine remained high and decreased slightly at midday, whereas in roots a continual drop occurred. In all organs examined, changes in glutamine reflected the double maxima of total soluble nitrogen and were greatest in roots.On a fresh weight basis, most amino acids accumulated at sunrise and near sunset; however a few especially in leaves, increased at midday, e.g. glutamic and aspartic acid, lysine, γ-aminobutyric acid, and serine.Comparison of levels of free guanidino compounds in different organs showed remarkable out-of-phase patterns. Levels of these compounds are known from 14C-arginine studies to be closely related to the metabolism of arginine.

A comparison of preharvest and postharvest ethylene production and respiration rates of saskatoon (Amelanchier alnifolia Nutt.) fruit during development

1999 ◽  
Vol 77 (3) ◽  
pp. 323-332
Author(s):  
Suzy Y Rogiers ◽  
N Richard Knowles
Keyword(s):  
Ethylene Production ◽  
Color Change ◽  
Wound Response ◽  
Weight Basis ◽  
Continuous Treatment ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Amelanchier Alnifolia ◽  
Respiration Rates ◽  
Maturity Class

Changes in respiration and ethylene production rates of nine maturity classes of saskatoon (Amelanchier alnifolia, Nutt.) fruit after harvest were compared with those of fruit maturing and ripening on the plant. During ripening on the plant, ethylene production increased on a whole-fruit and fresh-weight basis, while respiration increased substantially on a whole-fruit basis but remained constant on a fresh-weight basis. Fruit harvested at the greenish yellow to pink stages (maturity classes four and five, respectively) increased their ethylene production until 15-20 h after harvest, and this was coincident with ripening to maturity class seven and thus a color change to red. Ethylene production then declined over the next 15 h. Respiration rates of harvested immature, mature, and ripe fruits declined over 5 days, except for a relatively brief 8-12% rise starting 15-20 h after harvest. This brief increase in respiration was characteristic of fruit of all maturity classes, likely reflecting a wound response associated with harvest rather than a ripening-induced change in metabolism. Continuous treatment of attached or detached fruit with propylene or ethylene in an open airflow system did not induce an increase in respiration earlier, relative to control fruit. The respiratory response of saskatoon fruit during postharvest ripening was thus very different from that of fruit that ripened on the plant. Thus, the increase in respiration associated with ripening could only be demonstrated on a whole-fruit basis if fruit remained on the plant. Preharvest and postharvest changes in ethylene production during ripening also differed but were both consistent with climacteric ripening.Key words: Amelanchier alnifolia, ethylene, ripening, saskatoon fruit.

scholarly journals Macro and Micro Elemental Analysis of Anethum Sowa L. (Dill) Stem By X-Ray Fluorescence Spectrometry

1970 ◽  
Vol 43 (4) ◽  
pp. 483-494 ◽  
Author(s):  
M Moshfekus Saleh-e-in ◽  
Abida Sultana ◽  
M Abul Hossain ◽  
Mainul Ahsan ◽  
Sudhangshu Kumar Roy
Keyword(s):  
Elemental Concentration ◽  
Pharmaceutical Preparation ◽  
Weight Basis ◽  
Fresh Weight ◽  
Mineral Contents ◽  
Fresh Weight Basis ◽  
Livestock Feed ◽  
Micro Elements ◽  
Anethum Sowa ◽  
Proximate Analyses

Anethum sowa L. (Dill) stem portions were analyzed for its macro and micro elemental concentration by XRF spectrometry collected from different places of Bangladesh. The plants were collected when those were 35 cm in height i.e. within 40-45 days of sowing. Results of proximate analyses showed that the stem parts contained highest level of moisture (93.67), ash (1.62) and dry matter (30.77%) on fresh weight basis (g/100g). The highest level of mineral contents were Ca(478.50±6.22), K(336.65±3.26), Cl(153.92±3.26), Na(114.86±1.38), S(96.65±1.76), Mg(63.96±1.15), P(49.41±0.74), Al(41.75±0.71), Fe(34.57±1.33), Si(31.03±0.18), Ni (11.98±0.22) and Ti (3.75±0.28) in mg/100g on fresh weight basis. Cu, Sr, Rb, Zn, Ba and Zr were present in small amount and Y, Cu and Mn were present in trace amount. However, the toxic elements like Co, Pb, As and Hg were not found in the present study. Presence of higher amounts of mineral contents in the stem parts could potentially be consumed as supplement as human diets or livestock feed as well as pharmaceutical preparation. Key words: Anethum sowa L., XRF spectrometry, Dill, Condiment, Macro and micro elements, Elemental composition.     doi: 10.3329/bjsir.v43i4.2238   Bangladesh J. Sci. Ind. Res. 43(4), 483-494, 2008

Distribution of amylase and chymotrypsin in four regions of the rat pancreas

1981 ◽  
Vol 59 (8) ◽  
pp. 884-886 ◽  
Author(s):  
Adrien R. Beaudoin ◽  
Pierre St-Jean ◽  
Ginette Girard
Keyword(s):  
Protein Content ◽  
Acinar Cell ◽  
Dna Content ◽  
Pancreatic Tissue ◽  
Weight Basis ◽  
Secretory Proteins ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Rat Pancreas

Recent observations have confirmed the existence of more than one pool of secretory proteins in the rat pancreatic tissue. To determine if these different pools could be located in the different regions of the rat pancreas, the amylase and chymotrypsin contents have been measured in the biliary, duodenal, gastric, and splenic regions. On a tissue DNA content, protein content, or a fresh weight basis, the proportions of these two enzymes are comparable in the four regions. It is therefore postulated that heterogeneity of enzyme composition exists either within these regions or within the acinar cell itself.

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