Separation of Glyphosate and Possible Metabolites by Thin-Layer Chromatography

Weed Science ◽  
1978 ◽  
Vol 26 (6) ◽  
pp. 673-674 ◽  
Author(s):  
Paul Sprankle ◽  
C. L. Sandberg ◽  
W. F. Meggitt ◽  
Donald Penner
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Convolvulus Arvensis ◽  
Aminomethylphosphonic Acid ◽  
Field Bindweed ◽  
Layer Chromatography

Glyphosate [N-(phosphonomethyl)glycine] was separated from its potential metabolites, aminomethylphosphonic acid, glycine, and sarcosine by using 500 μm-thick cellulose plates developed with ethanol:water:15 N NH4OH:trichloroacetic acid (TCA):17 N acetic acid (55:35: 2.5:3.5 g:2, v/v/v/w/v with v in ml). This TLC system separated impurities from the14C-glyphosate standard and glyphosate from possible metabolites in treated field bindweed(Convolvulus arvensisL.).

scholarly journals A validated quantification of triclosan in toothpaste using high-performance thin-layer chromatography and a 48-bit fatbed scanner

2021 ◽  
Author(s):  
Barbara Anders ◽  
Sabrina Doll ◽  
Bernd Spangenberg
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Detection System ◽  
Flatbed Scanner ◽  
Butyl Ether ◽  
Quantification Method ◽  
Direct Measurements ◽  
Layer Chromatography

AbstractWe present a densitometric quantification method for triclosan in toothpaste, separated by high-performance thin-layer chromatography (HPTLC) and using a 48-bit flatbed scanner as the detection system. The sample was band-wise applied to HPTLC plates (10 × 20 cm), with fluorescent dye, Merck, Germany (1.05554). The plates were developed in a vertical developing chamber with 20 min of chamber saturation over 70 mm, using n-heptane–methyl tert-butyl ether–acetic acid (92:8:0.1, V/V) as solvent. The RF value of triclosan is hRF = 22.4, and quantification is based on direct measurements using an inexpensive 48-bit flatbed scanner for color measurements (in red, green, and blue) after plate staining with 2,6-dichloroquinone-4-chloroimide (Gibbs' reagent). Evaluation of the red channel makes the measurements of triclosan very specific. For linearization, an extended Kubelka–Munk expression was used for data transformation. The range of linearity covers more than two orders of magnitude and is between 91 and 1000 ng. The separation method is inexpensive, fast and reliable.

Dynamics of an Ester of 2,4-D in Organs of Three Fish Species

Weed Science ◽  
1972 ◽  
Vol 20 (1) ◽  
pp. 101-105 ◽  
Author(s):  
Charles A. Rodgers ◽  
David L. Stalling
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Fish Species ◽  
Metabolic Products ◽  
Dichlorophenoxy Acetic Acid ◽  
Layer Chromatography

The uptake of 14C-labeled butoxyethanol ester of (2,4-dichlorophenoxy)acetic acid (BEE of 2,4-D) from water by three species of fed and fasted fish was studied. Fish were exposed to either 0.3 or 1.0 mg/L of herbicide for up to 168 hr. Combined residues of the 2,4-D ester and its metabolic products were determined radiometrically in eight tissues and organs. Extracts of these organs were examined by thin layer chromatography and autoradiography. Maximum residue concentrations were observed in most organs of fed fish within 1 to 2 hr of exposure, and within 1 to 8 hr of exposure for fasted fish. After maximum residue concentrations were reached, the herbicide and/or its metabolites were eliminated rapidly.

Detection of Cashew Nut Shells in Coffee, Tea, and Chicory

1974 ◽  
Vol 57 (3) ◽  
pp. 761-762
Author(s):  
Pratima Sengupta ◽  
Asit R Sen ◽  
Nomita Ghoshdastidar ◽  
Bidhan R Roy
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Ether Extract ◽  
Cashew Nut ◽  
The Common ◽  
Cashew Nut Shell ◽  
Layer Chromatography ◽  
Ground Coffee

Abstract Thin layer chromatography has been investigated for the identification of cashew nut shell, one of the common adulterants in ground coffee, tea, and chicory. The ether extract of the sample is applied to silica gel C plates and developed with benzene-dioxane-acetic acid (90+25+4) . Cashew nut shell shows 3 distinctive spots (Rf 0.7, 0.54, and 0.34) with diazotized benzidine which are totally absent in tea, coffee, and chicory. The spots have been identified as anacardic acid, cardol, and anacardol, respectively.

3'-O-Formyl-(N-acyl)-2'-deoxyribonucleosides as building units in the synthesis of oligodeoxyribonucleotides

1982 ◽  
Vol 47 (8) ◽  
pp. 2157-2169 ◽  
Author(s):  
Jiří Smrt
Keyword(s):  
Acetic Acid ◽  
Acetic Anhydride ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Subsequent Treatment ◽  
Aqueous Acetic Acid ◽  
Formyl Group ◽  
Layer Chromatography ◽  
Methanolic Ammonia

5'-O-Dimethoxytrityl-(N-acyl)-2'-deoxyribonucleosides afford 3'-O-formyl-(N-acyl)-2'-deoxyribonucleosides Ia-Id by the action of formic acetic anhydride followed by the action of 80% aqueous acetic acid. The formyl group is removed from Ia-Id by treatment with 1 mol l-1 triethylamine 3'-O-Formyl-2'-deoxythymidine (Ia) gives 3'-O-dimethoxytrityl-2'-deoxythymidine (V) by subsequent treatment with acetic anhydride, triethylamine, dimethoxytrityl chloride and methanolic ammonia. The use of compounds I for the synthesis of d-GGAGG (XIX) and d-T16 (XXXII) is described. Systems for thin-layer chromatography of 5'-O-dimethyltrityl-oligodeoxyribonucleotides on silica gel are described.

scholarly journals Phytochemical Investigation of the Aerial Part of Iraqi Convolvulus arvensis

2020 ◽  
Vol 29 (2) ◽  
pp. 62-69
Author(s):  
Mustafa H. Alwan ◽  
Maha N. Hamad
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Mass Spectroscopy ◽  
Morning Glory ◽  
Convolvulus Arvensis ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Layer Chromatography

  Convolvulus arvensis is a species of bindweed that is rhizomatous and is in the morning glory family (Convolvulaceae) native to Europe and Asia. The plant is naturally grown in Iraq. The plant was reported to be used in traditional medicine from as early as 1730s. The Aerial parts of Convolvulus arvensis were macerated in 80% ethanol for 6 days. The concentrated extract was partitioned with n-hexane, chloroform, ethyl acetate- and n-butanol successively. The n-hexane and ethyl acetate, fractions were examined for the presence of phytochemicals by thin layer chromatography and high performance liquid chromatography and its steroid and flavonoid contents were investigated. Stigmasterol was isolated from n-hexane fraction and identified by liquid chromatography/mass spectroscopy. Rutin was isolated from the ethyl acetate fraction and identified by liquid chromatography/mass spectroscopy. The aim is to examine the phytochemical constituents of the aerial parts of Convolvulus arvensis, literature survey available so far revealed that there were no studies about the phytochemical investigation for Convolvulus arvensis in Iraq.  Different chromatographic techniques like Thin Layer Chromatography and mass spectroscopy were used and the presence of Stigmasterol and Rutin in aerial parts of Convolvulus arvensis was indicated.  

Susceptibility of Strawberries, Blackberries, and Cherries to Aspergillus Mold Growth and Aflatoxin Production

1982 ◽  
Vol 65 (3) ◽  
pp. 659-664 ◽  
Author(s):  
Gerald C Llewellyn ◽  
Thomas Eadie ◽  
William V Dashek
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mycelial Growth ◽  
Methylene Chloride ◽  
Aflatoxin Production ◽  
Solvent System ◽  
Mold Growth ◽  
Tlc Plates ◽  
Layer Chromatography

Abstract The susceptibility of blackberries, cherries, and strawberries to Aspergillus growth and aflatoxin production has been examined. Three aflatoxigenic isolates of Aspergillus, A. flavus ATCC 15548 and NRRL 3251 as well as A. parasiticus NRRL 2999, were cultured on homogenates of the fruits for 14 days at 28 ± 2°C. Percent mycelial growth and spore infestation were determined each day with a calibrated grid. At day 14 each culture was frozen at –5°C until aflatoxins were extracted with methylene chloride and water. Aflatoxins were separated by thin layer chromatography (TLC) with benzene-methanol-acetic acid (90 + 5 + 5). This extraction and solvent system provided satisfactory separations of the aflatoxins and was free of background interference on the TLC plates. Although all fruits served as substrates for both Aspergillus growth and aflatoxin production, cherries appeared to be a more favorable substrate than did blackberries, and the latter was more favorable than strawberries. Whereas A. flavus produced both B1 and G1 on all substrates, it yielded B2 and G2 only on cherries. Although A. parasiticus NRRL 2999 synthesized B1, B2, G1, and G2 on both blackberries and cherries, no aflatoxins were detected on strawberries. In contrast, A. flavus NRRL 3251 failed to produce detectable levels of aflatoxin on any substrate. All substrates supported both mycelial growth and subsequent sporulation with cherries > blackberries > strawberries.

Identifikasi Senyawa Jamu Pegal Linu yang Beredar di Kabupaten Bantul dengan Metode Kromatografi Lapis Tipis

2019 ◽  
Vol 14 (2) ◽  
pp. 80
Author(s):  
Furijika Fitriana Mosy ◽  
Kuswandani Kuswandani
Keyword(s):  
Acetic Acid ◽  
Herbal Medicine ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Random Sampling ◽  
Uv Light ◽  
Physicochemical Method ◽  
Layer Chromatography

Background: Traditional herbal is not permitted to contain chemicals drug. It is possible allowed such as paracetamol and phenylbutazone.Objective: This study is aimed to identify the compounds of paracetamol and phenylbutazone in the traditional herbal of Jamu Pegal Linu (Herbal Medicine).Methods: This type of research was descriptive and the sample was determined by random sampling. The method used Thin Layer Chromatography (TLC) which is a physicochemical method. There were eight samples were extracted by the soxhletation method until a thick extract was obtained to be spotted in the quite phase of TLC silica gel F254. The motion phase used for the analysis of paracetamol was chloroform: acetone: toluene (65:25:10) and the motion phase for phenylbutazone analysis was benzene: chloroform: 96% acetic acid (50:40:10). Spots detection was done by observing under UV light of 254 nm and the spots that appeared were calculated for Rf value and compared with the standard Rf value of paracetamol and phenylbutazone.Results: The results obtained in this study were positive E samples containing paracetamol and phenylbutazone with Rf value of sample 0.57 and a standard Rf of paracetamol 0.57 and an Rf value of sample 0.82 and a standard Rf of phenylbutazone 0.86. The positive G sample contained paracetamol with a sample Rf value of 0.61 and a standard Rf value of paracetamol 0.68.Conclusion: From the eight samples of ‘Jamu Pegal Linu’, two of them were positive containing chemical drugs paracetamol and phenylbutazone.Keywords: Jamu Pegal Linu (Herbal Medicine), Paracetamol, Phenylbutazone, TLC

European Taxa of Saxicolous Lecidella Containing Chloroxanthones:Identification of Patterns Using Thin Layer Chromatography

1992 ◽  
Vol 24 (4) ◽  
pp. 383-397 ◽  
Author(s):  
Ch. Leuckert ◽  
J.-G. Knoph
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Glacial Acetic Acid ◽  
Uv Light ◽  
Rf Values ◽  
Layer Chromatography ◽  
Spray Reagents

AbstractA method using TLC to recognize compound patterns consisting almost entirely of chloroxanthones in European saxicolous species of Lecidella is described. These are necessary for diagnosis of the taxa studied and of their chemotypes. Two solvents with complementary separatingqualities were required: C, toluene-glacial acetic acid (100:15); J, dichloromethane-acetone (4:1). Although the Rf values in solvent J strongly depend on the concentration of the substances, it is very suitable for identification because the xanthones in this system reveal distinct and characteristic fluorescence colours in UV light, simplifying interpretation enormously. No spray reagents are used. Identifications are made by co-chromatography with authentic lichencompounds

Enantioselective analysis of naproxen using chiral molecular imprinting polymers based thin-layer chromatography

e-Polymers ◽  
2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Fengyun Huangfu ◽  
Bing Wang ◽  
Juanjuan Shan ◽  
Zhiliang Zhang
Keyword(s):  
Particle Size ◽  
Acetic Acid ◽  
Particle Size Distribution ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Size Distribution ◽  
Acid Content ◽  
Rational Design ◽  
Acetic Acid Content ◽  
Layer Chromatography

Abstract This paper describes a rational design and testing of molecularly imprinted polymers (MIPs) as chiral stationary phases of thin-layer chromatography (TLC) for enantiomeric purity of naproxen. Using D-naproxen as template, MIPs with particle size between 10~90 μm were prepared by precipitation polymerization in acetonitrile/methanol mixed solvent. The interactions between functional monomers and template were verified by UV absorption spectrometry. The morphology, particle size distribution and specific surface area of MIPs were also observed by scanning electron microscopy, particle size distribution meter and liquid nitrogen adsorption instrument, respectively. Binding capacities of MIPs had been studied by equilibrium binding assay. Preparation conditions of TLC and impact of acetic acid content on the separation of enantiomers were investigated. The results indicated that when acetic acid content was 4%, the racemates of templates were completely separated, and the chiral separation factor α was 1.58.

Simplified Qualitative Analysis of Glycerides Derived from Coconut Oil Using Thin Layer Chromatography

2012 ◽  
Vol 506 ◽  
pp. 182-185 ◽  
Author(s):  
Sirikarn Pengon ◽  
Chutima Limmatvapirat ◽  
Sontaya Limmatvapirat
Keyword(s):  
Acetic Acid ◽  
Qualitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Cocos Nucifera ◽  
Coconut Oil ◽  
Solvent System ◽  
Tlc Plates ◽  
Solvent Systems ◽  
Layer Chromatography

Coconut (Cocos nucifera L.) oil is composed predominately of medium-chain triglycerides which have been reported to be beneficial to human health. It also contains free fatty acids (FFAs) which can combine with glycerol to form monoglycerides, diglycerides, and triglycerides. The analysis of FFAs and their glycerides has been proposed to assess the quality of coconut oil used as raw materials in various industrial fields. The aim of this study was to develop the qualitative method for investigation of FFA and their glycerides in coconut oil using thin layer chromatography (TLC). Coconut oil and standards of FFA and their glycerides were chromatographed separately on Silica gel 60 F254 TLC plates using hexane: ether: acetic acid (60:40:1) and hexane: ethyl acetate: acetic acid (60:40:0.5) as solvent systems A and B, respectively. The spots on developing TLC plates were detected and compared using 254-nm UV light and iodine vapor. The results showed that the resolution of solvent system A was better than that of solvent system B. However, both solvent systems were used to confirm the results. The retention factor (Rf) values of the components were in good agreement with their polarity. This method should provide a guideline for qualitative analysis of coconut oil.

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