Use of the gas production technique to investigate responses of supplementing low quality forages. 2. In vivo interactions and comparison with in vitro parameters

1998 ◽  
Vol 22 ◽  
pp. 266-268
Author(s):  
A. H. Murray ◽  
A. Moss ◽  
C. D. Wood ◽  
D. I. Givens ◽  
M. Gill
Keyword(s):  
Major Part ◽  
Developed Countries ◽  
Poor Quality ◽  
Gas Production ◽  
Short Supply ◽  
Fibre Degradation ◽  
Maintenance Requirements ◽  
Micro Organisms

Ruminants in many less developed countries may consume poor quality roughages such as straws, stovers and senescent native pasture as a major part of their diet, particularly during the dry season when high-quality forages are in short supply. The majority of these roughages are high in fibre, low in protein and the intake of digestible nutrients often is not enough to meet maintenance requirements. Intake and digestibility of poor-quality roughages may be increased by supplementation. The response to supplementation can be attributed to an increase in the supply of nitrogen and/or readily fermentable carbohydrate, resulting in an increase in rumen cellulolytic micro-organisms and therefore enhanced fibre degradation.

Use of the gas production technique to investigate responses of supplementing low quality forages. 1. In vitro interactions

1998 ◽  
Vol 22 ◽  
pp. 92-94
Author(s):  
C. D. Wood ◽  
A. H. Murray ◽  
A. R. Moss ◽  
D. I. Givens
Keyword(s):  
Crop Residue ◽  
Crop Residues ◽  
Developed Countries ◽  
Production Method ◽  
Gas Production ◽  
Short Supply ◽  
Dry Seasons ◽  
In Vitro Interactions

Nitrogen-deficient fibrous crop residues are widely used as basal diets in less developed countries, particularly in dry seasons when alternative foods are often in short supply. One approach to improving animal performance on crop residue based diets is to include a supplement of improved quality food to provide fermentable protein and energy. There are no established in vitro methods for investigating interactions between foods but the in vitro gas production method shows promise in this regard (Prasad et al., 1994). This paper describes the interactions observed in vitro; an accompanying paper (Murray et al., 1998) describes in vivo responses to supplementation and relationships between in vitro and in vivo data.

Comparison of bovine rumen liquor and faeces as sources of micro-organisms for the in vitro gas production technique assessed using twelve graminaceous forages

1998 ◽  
Vol 1998 ◽  
pp. 68-68
Author(s):  
R. Mauricio ◽  
A.L. Abdalla ◽  
F.L. Mould ◽  
U.R. Altaf ◽  
T. Smith ◽  
...  
Keyword(s):  
Pressure Transducer ◽  
Gas Production ◽  
Production Technique ◽  
Bovine Rumen ◽  
In Vitro Gas Production ◽  
Micro Organisms

The experiment was conducted using a range of forages with accurately predetermined OMD values (ADAS) to compare rumen liquor (RL) and faeces (FA) as sources of inocula in the pressure transducer technique (PTT) (Theodorou et al., 1994). Gas production results were examined in relation to OMD determined in vitro (PTT, Tilley and Terry) and in vivo.

scholarly journals Genotype and environment effects on feed grain quality

1999 ◽  
Vol 50 (5) ◽  
pp. 703 ◽  
Author(s):  
L. O'Brien
Keyword(s):  
Chemical Composition ◽  
Grain Quality ◽  
Nutritive Value ◽  
Protein Digestibility ◽  
Developed Countries ◽  
Gas Production ◽  
Environment Interaction ◽  
Environment Effects

The extent of genotype and location effects on chemical composition and nutritive value of grains fed to animals was surveyed. The review covered the winter cereals (wheat, barley, oats, and triticale), the summer cereals (sorghum and maize), and the pulses (field pea, lupins, faba beans, and chickpea) when fed to cattle, sheep, poultry, pigs, rats, and mice. The bulk of the literature does not meet the statistical criteria required to differentiate genotype and environment effects. When the criteria were satisfied, significant genotype differences were shown to exist for chemical composition in wheat, barley, triticale, and sorghum, for nutritive value as determined by methods in vitro in wheat, barley, oats, triticale, and sorghum, and in vivo for wheat, barley, triticale, sorghum, and maize. Valid comparisons across grain species are few, but in vitro gas production ranks wheat > oats > barley. Significant location, year, genotype × location, genotype × year, and genotype × location × year effects were reported for nutritive value for some grains. Wheat feeding trials with poultry indicate that environment can affect apparent metabolisable energy (AME) as much as, if not more than, genotype. A greater range in nutritive value appears to exist in barley than in wheat. The information is unclear in the case of triticale, where despite some reports claiming that grain of this species has high lysine content, the difference does not appear to translate to improved performance in animals. Insufficient studies exist for oats despite it being one of the most widely used on-farm feed grains. No examples could be found of studies with rye. The most thoroughly researched grain has been sorghum, which is principally grown in developed countries for feeding to livestock. Here, some definitive studies have been conducted to define the extent of genotype, location, and genotype × environment interaction effects. Scope exists to enhance the nutritive value of sorghum by breeding through modification of endosperm composition, tannin content, and improved protein digestibility. Variation in endosperm composition in maize due to simply inherited mutations provides the opportunity to improve its nutritive value. This review indicates that before any plant breeding is undertaken for feed grain quality, a better understanding of what determines nutritive value and the relative importance of genotype and environment in modulating these factors is required.

Comparison of bovine rumen liquor and faeces as sources of micro-organisms for the in vitro gas production technique assessed using twelve graminaceous forages

1998 ◽  
Vol 1998 ◽  
pp. 68-68 ◽  
Author(s):  
R. Mauricio ◽  
A.L. Abdalla ◽  
F.L. Mould ◽  
U.R. Altaf ◽  
T. Smith ◽  
...  
Keyword(s):  
Pressure Transducer ◽  
Gas Production ◽  
Production Technique ◽  
Bovine Rumen ◽  
In Vitro Gas Production ◽  
Micro Organisms

The experiment was conducted using a range of forages with accurately predetermined OMD values (ADAS) to compare rumen liquor (RL) and faeces (FA) as sources of inocula in the pressure transducer technique (PTT) (Theodorou et al., 1994). Gas production results were examined in relation to OMD determined in vitro (PTT, Tilley and Terry) and in vivo.

A history ofin vitrotechniques

1998 ◽  
Vol 22 ◽  
pp. 13-19
Author(s):  
D. J. Minson
Keyword(s):  
Continuous Fermentation ◽  
In Vitro Digestion ◽  
Food Samples ◽  
Gas Production ◽  
In Vitro Techniques ◽  
Dry Matter Digestibility ◽  
Calibration Methods ◽  
Micro Organisms

AbstractRegressions relating in vivo digestibility to chemical composition of the food have residual standard deviations that are unacceptably high. The development of the two-stage in vitro technique inoculated with rumen liquor (Tilley and Terry, 1963) allowed dry-matter digestibility to be predicted with greater accuracy. This success was followed by a series of developments which replaced rumen liquor with inoculum produced from fresh or preserved faeces collected from sheep or cattle. Other methods used inoculum from a continuous fermentation containing rumen micro-organisms and enzymes produced by fungi. Another modification was to use gas production as a measure of in vitro digestion. The range of nutritional problems that could be measured by in vitro techniques was extended to include the estimation of voluntary food intake and protein degradation. All these in vitro techniques require standardization using food samples that have previously been analysed in vitro or offered as the sole diet to animals. The relative merits of these two calibration methods are discussed. Special facilities are required for storing and distributing these standard foods.

scholarly journals Digestibility and rumen fermentation of a high forage diet pre-treated with a mixture of cellulase and xylanase enzymes

2021 ◽  
Vol 51 (3) ◽  
pp. 399-406
Author(s):  
K. Selzer ◽  
A. Hassen ◽  
A.M. Akanmu ◽  
A.Z.M. Salem
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Poor Quality ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Ruminal Fermentation ◽  
Forage Digestibility

Forages play an important role in ruminant animal production worldwide. Unlocking the nutritional potential of poor-quality tropical forages with fibrolytic enzymes would improve forage digestibility and utilization. Using in vitro and in vivo methods this study investigated the effect of pre-treating Smutsfinger hay for 24 hours with a mixture of fibrolytic enzyme (100% cellulase; 75% cellulase: 25% xylanase; 50% cellulase: 50% xylanase; 25% cellulase: 75% xylanase; 100% xylanase and a control with no enzyme) on ruminal fermentation and digestibility of nutrients by sheep. For in vitro fermentation, dry matter, neutral detergent fibre (NDF) degradability and volatile fatty acids (VFA) were determined with standard procedures. The same treatments were used for an in vivo digestibility trial using Merino sheep in a 6 x 6 Latin square design. Feed intake and total tract digestibility were recorded. Rumen fluid samples were collected daily, preserved, and analysed for VFA. The addition of 100% cellulase enzyme to Smutsfinger hay in vitro increased (P <0.05) NDF degradability and gas production compared with the control and inclusion of 100% xylanase enzyme. Both 100% cellulase and xylanase enzymes significantly reduced in vitro end time fermentation pH. A 50:50 mixture of cellulase and xylanase plus enzyme in vivo, increased acetate, total VFA concentration, and higher NDF and ADF digestibility of the test feed compared with the control. Inclusion of a 50-75% mixture of cellulase and 50-25% xylanase enzymes treatment led to higher gas production and butyrate concentration, decreased ruminal pH and improved nutrient digestibility.

Traditional Herbal Medicines, Newer Herbs and Other Novel Approaches Integrated in Herbal Medicine for Atopic Dermatitis-A Narrative Review

2020 ◽  
Vol 15 (3) ◽  
pp. 194-208
Author(s):  
Pravin Kumar ◽  
Dinesh Kumar Sharma ◽  
Mahendra Singh Ashawat
Keyword(s):  
Atopic Dermatitis ◽  
Herbal Medicines ◽  
Developed Countries ◽  
Skin Lesions ◽  
Scientific Data ◽  
Herbal Drugs ◽  
Biological Drugs ◽  
Alternative Treatment Option

Atopic Dermatitis (AD) is a prolonged reverting skin ailment with characteristically distributed skin lesions. In the previous decades, researchers had shown a marked interest in AD due to its increased prevalence in developed countries. Although different strategies including biological and immune modulators are available for the treatment of AD, each has certain limitations. The researchers had shown considerable interest in the management of AD with herbal medicines. The establishment of herbal drugs for AD might eliminate local as well as systemic adverse effects associated with long term use of corticosteroids and also higher cost of therapy with biological drugs. The present review discusses the traditional East Asian herbal medicines and scientific data related to newer herbal extracts or compositions for the treatment of AD. In vivo animal models and in vitro cell cultures, investigated with herbal medicines to establish a possible role in AD treatment, have also been discussed in the paper. The paper also highlights the role of certain new approaches, i.e. pharmacopuncture, a combination of allopathic and herbal medicines; and novel carriers (liposomes, cubosomes) for herbal drugs on atopic skin. In conclusion, herbal medicines can be a better and safe, complementary and alternative treatment option for AD.

Potential Antiulcer Agents From Plants: A Comprehensive Review

2019 ◽  
Vol 5 (3) ◽  
pp. 170-231
Author(s):  
Minky Mukhija ◽  
Bhuwan Chandra Joshi
Keyword(s):  
Medicinal Plants ◽  
Developed Countries ◽  
Scientific Data ◽  
Antiulcer Activity ◽  
Peptic Ulcers ◽  
Comprehensive Review ◽  
Chemical Structures ◽  
Mucosal Thickness

Background: Peptic ulcer is a deep gastrointestinal erosion disorder that involves the entire mucosal thickness and can even penetrate the muscular mucosa. Nowadays, several plants and compounds derived from it have been screened for their antiulcer activity. In the last few years, there has been an exponential growth in the field of herbal medicine. This field has gained popularity in both developing and developed countries because of their natural origin and less side effects. Objective: This review aims to provide a comprehensive summary of currently available knowledge of medicinal plants and phytoconstituents reported for their anti-ulcer properties. Methods: The worldwide accepted database like SCOPUS, PUBMED, SCIELO, NISCAIR, ScienceDirect, Springerlink, Web of Science, Wiley, SciFinder and Google Scholar were used to retrieve available published literature. Results: A comprehensive review of the present paper is an attempt to list the plants with antiulcer activity. The review narrates the dire need to explore potential chemical moieties that exert an antiulcer effect, from unexploited traditional plants. Furthermore, the present study reveals the intense requirement to exploit the exact mechanism through which either the plant extracts or their active constituents exhibit their antiulcer properties. Conclusion: This article is the compilation of the plants and its constituents reported for the treatment of peptic ulcers. The Comprehensive data will surely attract the number of investigators to initiate further research that might lead to the drugs for the treatment of ulcers. As sufficient scientific data is not available on plants, most of the herbals cannot be recommended for the treatment of diseases. This can be achieved by research on pure chemical structures derived from plants or to prepare new lead compounds with proven beneficial preclinical in vitro and in vivo effects. However, a lot remains to be done in further investigations for the better status of medicinal plants.

scholarly journals G2-S16 Polyanionic Carbosilane Dendrimer Can Reduce HIV-1 Reservoir Formation by Inhibiting Macrophage Cell to Cell Transmission

2021 ◽  
Vol 22 (16) ◽  
pp. 8366
Author(s):  
Ignacio Relaño-Rodríguez ◽  
María de la Sierra Espinar-Buitrago ◽  
Vanessa Martín-Cañadilla ◽  
Rafael Gómez-Ramírez ◽  
María Ángeles Muñoz-Fernández
Keyword(s):  
T Cells ◽  
Developed Countries ◽  
Main Role ◽  
Viral Particles ◽  
Carbosilane Dendrimer ◽  
Science Community ◽  
New Compounds ◽  
Hiv 1

Human immunodeficiency virus (HIV-1) is still a major problem, not only in developing countries but is also re-emerging in several developed countries, thus the development of new compounds able to inhibit the virus, either for prophylaxis or treatment, is still needed. Nanotechnology has provided the science community with several new tools for biomedical applications. G2-S16 is a polyanionic carbosilane dendrimer capable of inhibiting HIV-1 in vitro and in vivo by interacting directly with viral particles. One of the main barriers for HIV-1 eradication is the reservoirs created in primoinfection. These reservoirs, mainly in T cells, are untargetable by actual drugs or immune system. Thus, one approach is inhibiting HIV-1 from reaching these reservoir cells. In this context, macrophages play a main role as they can deliver viral particles to T cells establishing reservoirs. We showed that G2-S16 dendrimer is capable of inhibiting the infection from infected macrophages to healthy T CD4/CD8 lymphocytes by eliminating HIV-1 infectivity inside macrophages, so they are not able to carry infectious particles to other body locations, thus preventing the reservoirs from forming.

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