Variations in Surface Textures of Quartz Sand using Electron Microscopy

1970 ◽  
Vol 28 ◽  
pp. 494-495
Author(s):  
Eugene J. Amaral
Keyword(s):  
Electron Microscopy ◽  
Glass Industry ◽  
Glass Slide ◽  
Distilled Water ◽  
Early Paleozoic ◽  
Secondary Effects ◽  
Surface Textures ◽  
High Silica ◽  
Sand Deposit ◽  
The U.S

Examination of sand grain surfaces from early Paleozoic sandstones by electron microscopy reveals a variety of secondary effects caused by rock-forming processes after final deposition of the sand. Detailed studies were conducted on both coarse (≥0.71mm) and fine (=0.25mm) fractions of St. Peter Sandstone, a widespread sand deposit underlying much of the U.S. Central Interior and used in the glass industry because of its remarkably high silica purity.The very friable sandstone was disaggregated and sieved to obtain the two size fractions, and then cleaned by boiling in HCl to remove any iron impurities and rinsed in distilled water. The sand grains were then partially embedded by sprinkling them onto a glass slide coated with a thin tacky layer of latex. Direct platinum shadowed carbon replicas were made of the exposed sand grain surfaces, and were separated by dissolution of the silica in HF acid.

Scanning Electron Microscopy-cuticular Lesions on the Roundworm Ascaris Suum

1970 ◽  
Vol 28 ◽  
pp. 114-115
Author(s):  
P. A. Madden ◽  
W. R. Anderson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Freeze Drying ◽  
Room Temperature ◽  
Secondary Electron ◽  
Distilled Water ◽  
Freeze Dried ◽  
Silver Paint ◽  
To Come ◽  
Scanning Electron

The intestinal roundworm of swine is pinkish in color and about the diameter of a lead pencil. Adult worms, taken from parasitized swine, frequently were observed with macroscopic lesions on their cuticule. Those possessing such lesions were rinsed in distilled water, and cylindrical segments of the affected areas were removed. Some of the segments were fixed in buffered formalin before freeze-drying; others were freeze-dried immediately. Initially, specimens were quenched in liquid freon followed by immersion in liquid nitrogen. They were then placed in ampuoles in a freezer at −45C and sublimated by vacuum until dry. After the specimens appeared dry, the freezer was allowed to come to room temperature slowly while the vacuum was maintained. The dried specimens were attached to metal pegs with conductive silver paint and placed in a vacuum evaporator on a rotating tilting stage. They were then coated by evaporating an alloy of 20% palladium and 80% gold to a thickness of approximately 300 A°. The specimens were examined by secondary electron emmission in a scanning electron microscope.

Reconstruction of Two-Dimensional Projections of GP 32*I

1981 ◽  
Vol 39 ◽  
pp. 38-39
Author(s):  
H.A. Cohen ◽  
W. Chiu ◽  
J. Hosoda
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Uranyl Acetate ◽  
Distilled Water ◽  
Acetate Solution ◽  
Two Dimensional ◽  
Parent Molecule ◽  
T4 Bacteriophage ◽  
Electron Imaging ◽  
Better Than

GP 32 (molecular weight 35000) is a T4 bacteriophage protein that destabilizes the DNA helix. The fragment GP32*I (77% of the total weight), which destabilizes helices better than does the parent molecule, crystallizes as platelets thin enough for electron diffraction and electron imaging. In this paper we discuss the structure of this protein as revealed in images reconstructed from stained and unstained crystals.Crystals were prepared as previously described. Crystals for electron microscopy were pelleted from the buffer suspension, washed in distilled water, and resuspended in 1% glucose. Two lambda droplets were placed on grids over freshly evaporated carbon, allowed to sit for five minutes, and then were drained. Stained crystals were prepared the same way, except that prior to draining the droplet, two lambda of aqueous 1% uranyl acetate solution were applied for 20 seconds. Micrographs were produced using less than 2 e/Å2 for unstained crystals or less than 8 e/Å2 for stained crystals.

A rapid silver-impregnation technique on ultra-thin sections for Electron Microscopy

1993 ◽  
Vol 51 ◽  
pp. 242-243
Author(s):  
K. Chien ◽  
R.C. Heusser ◽  
M.L. Jones ◽  
R.L. Van de Velde
Keyword(s):  
Electron Microscopy ◽  
Silver Nitrate ◽  
Silver Impregnation ◽  
Sodium Borate ◽  
Renal Diseases ◽  
Distilled Water ◽  
Thin Sections ◽  
Impregnation Technique ◽  
Centrifuge Tube ◽  
Simplified Procedure

Silver impregnation techniques have been used for the demonstration of the complex carbohydrates in electron microscopy. However, the silver stains were believed to be technically sensitive and time consumming to perform. Currently, due to the need to more specifically evaluate immune complex for localization in certain renal diseases, a simplified procedure in conjunction with the use of the microwave has been developed and applied to renal and other biopsies. The procedure is as follows:Preparation of silver methenamine solution:1. 15ml graduated, clear polystyrene centrifuge tube (Falcon, No. 2099) was rinsed once with distilled water.2. 3% hexamethylene tetramine (methenamine) was added into the centrifuge tube to the 6ml mark.3. 3% silver nitrate was added slowly to the methenamine to the 7ml mark while agitating. (Solution will instantly turn milky in color and then clear rapidly by mixing. No precipitate should be formed).4. 2% sodium borate was added to the solution to the 8ml mark, mixed and centrifuged before use.

Life Beyond 1MeV

1980 ◽  
Vol 38 ◽  
pp. 30-33
Author(s):  
K.H. Westmacott
Keyword(s):  
Electron Microscopy ◽  
Past Experience ◽  
The Other ◽  
Good Case ◽  
Other Hand ◽  
The U.S

Life beyond 1MeV – like life after 40 – is not too different unless one takes advantage of past experience and is receptive to new opportunities. At first glance, the returns on performing electron microscopy at voltages greater than 1MeV diminish rather rapidly as the curves which describe the well-known advantages of HVEM often tend towards saturation. However, in a country with a significant HVEM capability, a good case can be made for investing in instruments with a range of maximum accelerating voltages. In this regard, the 1.5MeV KRATOS HVEM being installed in Berkeley will complement the other 650KeV, 1MeV, and 1.2MeV instruments currently operating in the U.S. One other consideration suggests that 1.5MeV is an optimum voltage machine – Its additional advantages may be purchased for not much more than a 1MeV instrument. On the other hand, the 3MeV HVEM's which seem to be operated at 2MeV maximum, are much more expensive.

scholarly journals First Report of Xylella fastidiosa on Catharanthus roseus in Brazil

Plant Disease ◽  
1998 ◽  
Vol 82 (6) ◽  
pp. 712-712 ◽  
Author(s):  
B. Ueno ◽  
C. K. Funada ◽  
M. A. Yorinori ◽  
R. P. Leite
Keyword(s):  
Catharanthus Roseus ◽  
Xylella Fastidiosa ◽  
Xylem Sap ◽  
Glass Slide ◽  
Bacterial Cells ◽  
First Report ◽  
Vein Clearing ◽  
The Media ◽  
Agar Media ◽  
The U.S

In 1998, plants of periwinkle (Catharanthus roseus L.) showing small leaves, short internodes, and dieback symptoms were observed in a garden at the Instituto Agronomico do Parana (IAPAR), Londrina, PR, Brazil. Stems of these plants were cut into short sections and the sap extracted from the tissue by squeezing with pliers. The sap was blotted onto a glass slide and examined for the presence of bacteria by light microscopy (×400). Microscopy observations revealed the presence of a large number of slender, rod-shaped bacterial cells. The bacteria present in the stems of periwinkle were isolated on buffered cysteine-yeast extract (BCYE) and periwinkle wilt (PW) agar media. Stems were disinfected in 70% alcohol and cut into short sections, and the sap extracted as described above. The sap was blotted directly onto the media and the plates were incubated at 28°C. Typical colonies of Xylella fastidiosa were observed 10 days after isolation on both media. Indirect immunofluorescence tests with antibody specific to X. fastidiosa and anti-IgG conjugated with tetrametylrhodamine isothiocyanate (TRITC) were carried out with xylem sap of periwinkle stem and the isolated bacteria. In both cases, immunofluorescence tests were positive for X. fastidiosa. These results confirm that periwinkle plants were infected with X. fastidiosa. This is the first report of the association of X. fastidiosa with periwinkle plants in Brazil. However, the symptoms observed for the X. fastidiosa-infected periwinkle plants differed from those described previously in the U.S. (1): those symptoms consisted of marginal chlorosis and occasional vein clearing of leaves and wilting of the plants. Reference: (1) R. E. McCoy et al. Plant Dis. Rep. 62:1022, 1978.

scholarly journals SEM, EDS and Electrical Capacitance Study on Electrodeposited Ni-Cu Layers

2019 ◽  
Vol 70 (9) ◽  
pp. 3210-3212
Author(s):  
Oana Claudia Ciobotea Barbu ◽  
Ioana Alina Ciobotaru ◽  
Anca Cojocaru ◽  
Florin Mihai Benga ◽  
Danut Ionel Vaireanu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Chemical Composition ◽  
Electrochemical Method ◽  
Steel Substrate ◽  
Distilled Water ◽  
Electrical Capacitance ◽  
Nickel Copper ◽  
Scanning Electron

Nickel-Copper metallic layers were deposited onto a steel substrate by using the electrochemical method. The morphology and the chemical composition of the deposited layers were studied by scanning electron microscopy. The electrical capacitance was measured on a functional supercapacitor made of two Ni-Cu deposited layers and a Nafion 117� membrane hydrated with distilled water, which served as a dielectric separator.

Ultrastructure of the Tertiary Envelope of the Cyst of the Tadpole Shrimp Triops longicaudatus (Branchiopoda; Notostraca)

2000 ◽  
Vol 6 (S2) ◽  
pp. 872-873
Author(s):  
James R. Rosowski ◽  
Terry L. Bartels ◽  
James F. Colburn ◽  
Jannell L. Colton ◽  
Denton Belk ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fairy Shrimp ◽  
Distilled Water ◽  
Thin Sections ◽  
Rainfall Events ◽  
Transmission Electron ◽  
Tadpole Shrimp ◽  
Species Specific ◽  
Scanning Electron

Tadpole shrimp inhabit temporary freshwater pools and ponds where their occurrence is largely regulated by rainfall events and water temperature. When dry basins are flooded, cysts of Triops imbibe water and hatch to produce rapidly growing, carapaced larvae. While previous studies show anostracan (fairy shrimp) cyst-surface morphology often species specific, few studies illustrate shell ultrastructure of Triops and none has considered T. longicaudatus. Here we examine the shell of T. longicaudatus (Notostraca) and compare its fine structure to other species of Triops and to that of Artemiafranciscana(Anostraca), which we previously studied.Cysts, produced in culture from Utah broodstock, were purchased from Triops, Inc., 1924 Creighton Rd., Pensacola, FL 32504. Thin sections of cysts were prepared for transmission electron microscopy (TEM) as previously described (Fig. 1). Cysts were also examined with scanning electron microscopy (SEM), dry, whole or fractured (Figs. 2,3), or after imbibition and/or hatching in oxygen saturated, double-distilled water, at 25 ° C.

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