Ultrastructure of the Tertiary Envelope of the Cyst of the Tadpole Shrimp Triops longicaudatus (Branchiopoda; Notostraca)

2000 ◽  
Vol 6 (S2) ◽  
pp. 872-873
Author(s):  
James R. Rosowski ◽  
Terry L. Bartels ◽  
James F. Colburn ◽  
Jannell L. Colton ◽  
Denton Belk ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fairy Shrimp ◽  
Distilled Water ◽  
Thin Sections ◽  
Rainfall Events ◽  
Transmission Electron ◽  
Tadpole Shrimp ◽  
Species Specific ◽  
Scanning Electron

Tadpole shrimp inhabit temporary freshwater pools and ponds where their occurrence is largely regulated by rainfall events and water temperature. When dry basins are flooded, cysts of Triops imbibe water and hatch to produce rapidly growing, carapaced larvae. While previous studies show anostracan (fairy shrimp) cyst-surface morphology often species specific, few studies illustrate shell ultrastructure of Triops and none has considered T. longicaudatus. Here we examine the shell of T. longicaudatus (Notostraca) and compare its fine structure to other species of Triops and to that of Artemiafranciscana(Anostraca), which we previously studied.Cysts, produced in culture from Utah broodstock, were purchased from Triops, Inc., 1924 Creighton Rd., Pensacola, FL 32504. Thin sections of cysts were prepared for transmission electron microscopy (TEM) as previously described (Fig. 1). Cysts were also examined with scanning electron microscopy (SEM), dry, whole or fractured (Figs. 2,3), or after imbibition and/or hatching in oxygen saturated, double-distilled water, at 25 ° C.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

scholarly journals Mitochondria of Human Adrenal Cortex Have Tubular Cristae with Bulbous Tips

2003 ◽  
Vol 88 (4) ◽  
pp. 1903-1906 ◽  
Author(s):  
Alessandro Riva ◽  
Felice Loffredo ◽  
Alessandro Uccheddu ◽  
Francesca Testa Riva ◽  
Bernard Tandler
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Adrenal Cortex ◽  
Thin Sections ◽  
Transmission Electron ◽  
Soluble Material ◽  
Bulbous Tip ◽  
Scanning Electron

By taking advantage of a modified osmium maceration technique, we have been able to examine by high resolution scanning electron microscopy (HRSEM) the interior of human adrenocortical mitochondria from which all soluble material has been extracted. The so-called vesicles apparent in thin sections examined by transmission electron microscopy actually are finger-like cristae as determined by HRSEM. These digitiform cristae have a segmented appearance and a bulbous tip. The segmented form of the cristae may have important metabolic implications.

Eucalypt phytoglyphs: The micronanatomical features of the epidermis in relation to taxonomy

1971 ◽  
Vol 19 (2) ◽  
pp. 173 ◽  
Author(s):  
SGM Carr ◽  
L Milkovits ◽  
DJ Carr
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Thin Sections ◽  
Hardening Process ◽  
Leaf Surfaces ◽  
The Status ◽  
Species Specific ◽  
High Degree ◽  
Scanning Electron

The eucalypt leaf contains a store of untapped information of potentially great value taxonomic and evututionary studies. Tie cuticie of certain eucalypts is shown to possess a complex and species-specific ornamentation so distinctive that its features can be regarded as diagnostic. The term "phytoglyph" is coined for the constellation of microanatomical features of the surfaces of leaves, including the microanatomy of the cuticle. Phytoglyphic analysis relates to the combination of three methods, light microscopy of stained cuticles, scanning electron microscopy of leaf surfaces, and light microscopy of thin sections of the cuticular and associated structures. Its use is illustrated by the dissection of the "form species" E. dichromophloia into a number of separate and recognizable entities, some of which were previously accorded the status of species. The plant geographical and other implications of this dissection are dealt with. In particular, E. dichromophloia F. Muell. is to be regarded as a species of very restricted distribution. The microanatomical characters of the cuticle are closely controlled products of the epidermal layers. The fact that specimens which (on other grounds) can be grouped together as a species have identical cuticular microanatomy suggests that the phytoglyph is genetically strongly determined and does not consist of inadvertent, trivial surface features with a high degree of plasticity. This in turn raises the problem of the development of the cuticular microanatomy which cannot be explained on current views of the formation of the cuticle by passive diffusion of precursor substances through the epidermal walls, followed by a hardening process.

scholarly journals Colloidal gold, a useful marker for transmission and scanning electron microscopy.

1977 ◽  
Vol 25 (4) ◽  
pp. 295-305 ◽  
Author(s):  
M Horisberger ◽  
J Rosset
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Yeast Cells ◽  
Erythrocyte Membranes ◽  
Optimum Conditions ◽  
Thin Sections ◽  
Good Spatial Resolution ◽  
Transmission Electron ◽  
Scanning Electron

Electron dense markers of a size suitable for transmission electron microscopy and scanning electron microscopy have been prepared with gold granules labeled with a monolayer of specific macromolecules. The optimum conditions for preparing the markers have been ascertained. The method is simple, rapid and seems to be general since gold granules have been labeled with polysaccharides and proteins. As homogeneous populations of gold granules having different sizes can be prepared, the method is also suitable for double marking experiments. The gold technique is illustrated by the localization of polysaccharides and glycoproteins on yeast cell walls and erythrocyte membranes by transmission electron microscopy and on yeast cells and intact erythrocytes by scanning electron microscopy. Good spatial resolution of the marker was achieved in all cases. The method is also suitable for marking thin sections. Spectrophotometric measurements were used to determine the number of gold granules adsorbed per cell.

scholarly journals Roaming of Materials Scientists in Biology: Structural Colours of Butterfly Wings

2019 ◽  
Vol 2 (2) ◽  
pp. 69-72
Author(s):  
László P. Biró ◽  
Krisztián Kertész ◽  
Gábor Piszter ◽  
Zsolt E. Horváth ◽  
Zsolt Bálint
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
General Plan ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Butterfly Wings ◽  
Species Specific ◽  
High Degree ◽  
Scanning Electron ◽  
Wing Scales

Abstract The photonic nanoarchitectures occurring in the wing scales of Lycaenid butterflies were investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and UV-VIS spectroscopy. We found that the males of all the nine investigated species possess photonic nanoarchitectures built according to the same general “plan”, but each species exhibits species-specific features which results in species-specific colours reproduced generation by generation with a high degree of accuracy.

Ultrastructure of the dormant and germinating sporangiospore of Phascolomyces articulosus (Mucorales)

1978 ◽  
Vol 56 (7) ◽  
pp. 747-753 ◽  
Author(s):  
P. Jeffries ◽  
T. W. K. Young
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Freeze Fracture ◽  
Spore Wall ◽  
Wall Layer ◽  
Thin Sections ◽  
Transmission Electron ◽  
Sporangial Wall ◽  
Scanning Electron

Using results obtained with light and scanning electron microscopy of critical-point-dried material and transmission electron microscopy of carbon replicas and freeze-fracture and ultra-thin sections, the structure and germination of the sporangiospore of Phascolomyces articulosus Boedijn is described. The sporangial wall is trilaminate and the ornamented spore wall is two layered. During germination, a new wall layer develops between the plasmalemma and the original spore wall. Sporangial structure is related to that of other members of the Thamnidiaceae and the use of germinating spores of P. articulosus for infection studies of the mycoparasite Piptocephalis unispora is indicated.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Scanning and Transmission Microscopy of Nematocyst Batteries in Epitheliomuscular Cells of Hydra

1971 ◽  
Vol 29 ◽  
pp. 410-411 ◽  
Author(s):  
Jane A. Westfall ◽  
S. Yamataka ◽  
Paul D. Enos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Osmium Tetroxide ◽  
External Surface ◽  
Three Dimensional ◽  
Surface Structures ◽  
Transmission Microscopy ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Scanning Electron

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).

Scanning Electron Microscopy-cuticular Lesions on the Roundworm Ascaris Suum

1970 ◽  
Vol 28 ◽  
pp. 114-115
Author(s):  
P. A. Madden ◽  
W. R. Anderson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Freeze Drying ◽  
Room Temperature ◽  
Secondary Electron ◽  
Distilled Water ◽  
Freeze Dried ◽  
Silver Paint ◽  
To Come ◽  
Scanning Electron

The intestinal roundworm of swine is pinkish in color and about the diameter of a lead pencil. Adult worms, taken from parasitized swine, frequently were observed with macroscopic lesions on their cuticule. Those possessing such lesions were rinsed in distilled water, and cylindrical segments of the affected areas were removed. Some of the segments were fixed in buffered formalin before freeze-drying; others were freeze-dried immediately. Initially, specimens were quenched in liquid freon followed by immersion in liquid nitrogen. They were then placed in ampuoles in a freezer at −45C and sublimated by vacuum until dry. After the specimens appeared dry, the freezer was allowed to come to room temperature slowly while the vacuum was maintained. The dried specimens were attached to metal pegs with conductive silver paint and placed in a vacuum evaporator on a rotating tilting stage. They were then coated by evaporating an alloy of 20% palladium and 80% gold to a thickness of approximately 300 A°. The specimens were examined by secondary electron emmission in a scanning electron microscope.

Copper Localization in Cirrhotic Rat Liver by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 38-39 ◽  
Author(s):  
J. C. Russ ◽  
E. McNatt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Copper Acetate ◽  
Lead Citrate ◽  
Dense Bodies ◽  
Transmission Electron ◽  
Electron Mode ◽  
Scanning Electron

In order to study the retention of copper in cirrhotic liver, rats were made cirrhotic by carbon tetrachloride inhalation twice weekly for three months and fed 0.2% copper acetate ad libidum in drinking water for one month. The liver tissue was fixed in osmium, sectioned approximately 2000 Å thick, and stained with lead citrate. The section was examined in a scanning electron microscope (JEOLCO JSM-2) in the transmission electron mode.Figure 1 shows a typical area that includes a red blood cell in a sinusoid, a disse, and a portion of the cytoplasm of a hepatocyte which contains several mitochondria, peribiliary dense bodies, glycogen granules, and endoplasmic reticulum.

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