Ultrastructural Changes of Gerbil Epididymis after Vasectomy

1977 ◽  
Vol 35 ◽  
pp. 672-673
Author(s):  
Shyamal K. Majumdar ◽  
Marion Shapiro ◽  
J. Gary Caputi
Keyword(s):  
Ventral Side ◽  
Uranyl Acetate ◽  
Meriones Unguiculatus ◽  
Ultrastructural Changes ◽  
Sham Operation ◽  
Mongolian Gerbils ◽  
Additional Information ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
One Year

Ultrastructural studies of gerbil testes after vasectomy have been described (2S3). The present study was undertaken to obtain additional information on the effects of vasectomy in the fine structures of the epididymis of the Mongolian gerbils (Meriones unguiculatus). The animals were subjected to vasectomy or sham operation at intervals up to one year. An incision was made on the ventral side of the animal just above the scrotum, and about one centimeter of each vas deferens was removed and the ends were firmly ligated. The cauda epididymis of vasectomized and sham operated animals was fixed for two hours in Karnovsky's fixative. Following a rinse in buffer, the tissue was post fixed for one hour in osmium tetroxide, dehydrated, and embedded in Epon 812. The sections were stained with uranyl acetate followed by Reynolds' lead citrate, and examined with a Philips 201 transmission electron microscope operating at 60 kV.

Electron Microscopic Studies of Gerbil Testis After Vasectomy

1976 ◽  
Vol 34 ◽  
pp. 154-155
Author(s):  
S. K. MAJUMDAR ◽  
FRED KALENSCHER
Keyword(s):  
Electron Microscope ◽  
Normal Structure ◽  
Uranyl Acetate ◽  
Meriones Unguiculatus ◽  
Mongolian Gerbils ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Acetate Lead ◽  
Microscopic Studies ◽  
Ultrathin Sections

Ultrathin sections made from bilaterally vasectomized as well as bilaterally sham-operated Mongolian gerbils (Meriones unguiculatus) were examined and compared under an electron microscope in order to determine whether vasectomy has any effect upon the fine structure of the testis. The whole testes were removed and placed in Karnovsky's fixative for one hour. After this period the testes were diced into small pieces and fixed for an additional hour in the same fixative. After rinsing in distilled water and postfixed for one hour in OsO4, the tissues were embedded in Epon 812. The sections were stained with uranyl acetate-lead citrate and examined on a Philips Model 201 transmission electron microscope. Shamoperated testis exhibited normal structure of germ cells.

Ultrastructural studies of cartilage in genetic disorders of skeletal growth

1978 ◽  
Vol 36 (2) ◽  
pp. 668-669
Author(s):  
D. O. Sillence ◽  
D. L. Rimoin ◽  
Ruth Silberberg
Keyword(s):  
Genetic Disorders ◽  
Electron Microscopic Examination ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Electron Microscopic ◽  
Skeletal Dysplasias ◽  
Ultrastructural Studies ◽  
Low Viscosity ◽  
Transmission Electron ◽  
Cacodylate Buffer

The human skeletal dysplasias are an heterogeneous group of heritable connective tissue disorders associated with abnormalities in the size and shape of the limbs, trunk and/or skull which frequently result in disproportionate short stature. In recent years it has become apparent that these comprise over 50 distinct conditions with a variety of subtypes distinguished on clinical and radiological grounds. We have investigated the pathogenesis of these conditions in over 100 patients by direct transmission electron microscopic examination of chondro-osseous tissue. Some of the ultrastructural studies have been previously reported.Small biopsies of chondro-osseous junction were collected for electron microscopy from the rib or iliac crest of patients with skeletal dysplasias or from normal controls at the time of surgery. These were cut into small blocks and fixed for one hour in either 5% glutaraldehyde in white's buffer or directly in 1% osmic acid in White's buffer or a modified Karnovsky's fixative, (2. 5% paraformaldehyde, 2. 5% glutaraldehyde, 2. 5mM calcium in cacodylate buffer). Subsequent processing included osmium fixation, block staining with uranyl acetate and embedding in Araldite or Spurr's low viscosity resin (firm composition). Sections were cut with glass knives or diamond knives. The latter produced sections which were much more even in thickness, permitting more consistent appraisal of matrix features.

Use of thin sections and freeze-etch replicas to differentiate the macrogametocyte from the microgametocyte in the malarial parasite Plasmodium falciparum

1994 ◽  
Vol 52 ◽  
pp. 280-281
Author(s):  
Charles A. M. Meszoely ◽  
Devi Venugopal ◽  
Eric F. Erbe ◽  
William P. Wergin
Keyword(s):  
Plasmodium Falciparum ◽  
Uranyl Acetate ◽  
Malarial Parasite ◽  
Thin Sections ◽  
Ultrastructural Studies ◽  
Cytoplasmic Organelles ◽  
Transmission Electron ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum ◽  
Freeze Etching

Gametocytes of Plasmodium falciparum grown in culture were chemically fixed in 2% glutaraldehyde. For observation of thin sections, specimens were postfixed in 1 % osmium tetroxide, dehydrated in ethanol, and embedded in resin. Thin sections were stained with uranyl acetate and lead citrate and examined with a Zeiss EM-10 transmission electron microscope (TEM). For observations of freeze-etched replicas, specimens were cryoprotected in 30% glycerol, fractured, etched, shadowed and coated in a modified Denton DFE-2 module. The replicas were examined with a JEOL JEM 100-B TEM.Previous ultrastructural studies on the gametocytes of many species of Plasmodium compared and contrasted the pellicular complex and cytoplasmic organelles, but differentiation of the macrogametocyte and the microgametocyte in freeze-etched preparations has not been reported. The intraerythrocytic macro- and microgametocytes can be easily differentiated by their staining properties in Giemsa stained preparations examined by light microscopy. The present study, using thin sectioning and freeze-etching techniques, differentiates the macrogametocyte from the microgametocyte of Plasmodium (Laverania) falciparum (Welch, 1897) by characterizing their cytoplasmic organelles.

Ultrastructural observations of the ciliated cyst in the human uterine tube epithelium

1990 ◽  
Vol 48 (3) ◽  
pp. 104-105
Author(s):  
Haruo Hagiwara ◽  
Susumu Shibasaki ◽  
Nobuo Ohwada
Keyword(s):  
Phosphate Buffer Solution ◽  
Uranyl Acetate ◽  
Buffer Solution ◽  
Solution Ph ◽  
Uterine Tube ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Gynecological Diseases ◽  
Microscopic Studies ◽  
Ultrathin Sections

The ciliated cysts have been identified as a common manner of development of ciliated cells through light microscopic studies, however recent ultrastructural studies have Reported that they should be regarded as an expression of atypical ciliogenesis. In the present study we attempt to elucidate the detailed structure of the ciliated cysts and their significance in ciliogenesis in the human uterine tube epithelium.Human uterine tubes were obtained from 20 women who underwent total hysterectomies due to gynecological diseases. The ampullar segments were cut into small pieces, and fixed in 2.5% glutaraldehyde-2% paraformaldehyde (1/2 Kamovsky) in a 0.1M phosphate buffer solution (pH 7.4). These specimens were postfixed in a 1% Osmium tetroxide solution (Millonig), dehydrated with a graded series of ethanol and embedded in Epon 812. Ultrathin sections were stained with uranyl acetate and lead citrate, and examined in a transmission electron microscope.The ciliated cysts were detected as a round or oval intracytoplasmic cavity provided with many ciliary apparatuses and microvilli (Fig. 1).

Changes in the plastid ultrastructure during greening in cultured soybean cells

1989 ◽  
Vol 47 ◽  
pp. 1010-1011
Author(s):  
M.A. Gillott ◽  
G. Erdös ◽  
D. E. Buetow
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Gene Regulation ◽  
Uranyl Acetate ◽  
Total Darkness ◽  
Ultrastructural Changes ◽  
Ethanol Dehydration ◽  
Mesophyll Cells ◽  
Plastid Ultrastructure ◽  
Transmission Electron

Mesophyll cells isolated from soybeans (Glycine max, L. Merr. var. Corsoy) can be grown photoautotrophically in suspension culture. The SB-P cell line can be bleached by maintaining them in total darkness in sucrose supplemented media for several weeks, and will regain photosynthetic competency when returned to the light. This system is ideally suited for the study of gene regulation and the biochemical and ultrastructural changes which occur during the greening process.Cells were fixed for electron microscopy after 8 weeks of growth in total darkness and at intervals of 1 h to 12 d after transfer to the light. Chlorophyll measurements were determined for each sample. For transmission electron microscopy, the cells were fixed for 1-2 h in 4% glutaraldehyde in 0.1M Pipes buffer, pH 7.4, washed in the same buffer, then postfixed for 1 h in 1% OsO4 in Pipes, pH 6.8. Following a graded ethanol dehydration series the cells were transferred into propylene oxide and embedded in Epon. Sections were stained with uranyl acetate and observed on a JEOL 100C TEM operated at 80 kV.

Effects of Soil Removal and Flooding on Leaf Cell Structure of Quercus Falcata Var. Pagodaefolia and Qurecus Nigra

1997 ◽  
Vol 3 (S2) ◽  
pp. 99-100
Author(s):  
Zhu Hua Ning ◽  
Kamran K. Abdollahi
Keyword(s):  
Soil Removal ◽  
Cell Structure ◽  
Leaf Tissue ◽  
Uranyl Acetate ◽  
Urban Trees ◽  
Thin Sections ◽  
Transmission Electron ◽  
Leaf Tissues ◽  
One Year ◽  
Water Oak

Soil removal and flooding are serious problems for many urban trees. The main purpose of this paper is to outline some changes which occur in leaves of cherrybark oak (Quercus falcata var. pagodaefolia) and water oak (Quercus nigra) during soil removal and flooding treatments. There has not been any prior documentation of leaf tissue of trees under these conditions. Two-year-old saplings of cherrybark oaks and water oaks were randomly planted in twenty-four experimental plots. Trees were randomly subjected to a combination of soil removal and flooding treatments for one year. Leaf tissues were collected from trees in controlled and treated plots. Tissues were fixed overnight at 4° C in 4% glutaraldehyde buffered with 0.07M cacodylate at pH of 7.2 and post-fixed for one hour in 1% osmium tetroxide in the same buffer. After washing thoroughly in the buffer, tissues were dehydrated in a graded ethanol series and embedded in Eponate 12 resin. Thin sections of 75nm thick were cut with a MT-6000 ultratome and placed on 300 mesh copper grids. Sections were then positive stained with uranyl acetate followed by lead citrate. All examinations of the cell structure were made using a Zeiss EM 10C transmission electron microscope.

Effects of gamma radiation on muscle ultrastructure and proteinase activity of grass shrimp (Penaeus monodon F.)

1990 ◽  
Vol 48 (3) ◽  
pp. 474-475
Author(s):  
Fang-Shiow Perng ◽  
Jui-Sen Yang
Keyword(s):  
Body Fluid ◽  
Penaeus Monodon ◽  
Grass Shrimp ◽  
Total Radioactivity ◽  
Uranyl Acetate ◽  
The Body ◽  
Proteinase Activity ◽  
Ultrastructural Changes ◽  
Transmission Electron ◽  
Irradiation Dose Rate

Effects of irradiation on ultrastructural changes and proteinase activity of grass shrimp during storage was investigated. Shrimps were irradiated by a 60Co radiation source (total radioactivity 143 KCi and irradiation dose rate 3.0 KGy/hr) at a dose of 40 KGy at frozen temperature. Tissue pieces from the first abdominal segment of the shrimps were primarily fixed in 4 % glutaraldehyde (in 0.1 M K-phosphate buffer containing 3.04 % NaCl and 0.02 % CaCl2, pH 7.2), postfixed in 1 % OsO4, and dehydrated in acetone series and embedded in Spurr’s resin. Sections were stained with uranyl acetate and lead citrate and examined in a Hitachi H-600 transmission electron microscope operated at 75 KV.Small pieces of muscle were dissected from living grass shrimps and immediately incubated with 5 ml of shrimp body fluid for 30 minutes. The body fluid was released from irradiated whole shrimps after storage at ambient temperature for three weeks. The muscle pieces were also incubated with crude proteinase extracted from irradiated hepatopancreas dissected from shrimps or proteinase (EC 3.4.4.16) from Bacillus licheniformis. After incubation the ultrastrucutre of the muscles was prepared for examination by electron microscopy.

scholarly journals MeHg Causes Ultrastructural Changes in Mitochondria and Autophagy in the Spinal Cord Cells of Chicken Embryo

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Fabiana F. Ferreira ◽  
Evelise M. Nazari ◽  
Yara M. R. Müller
Keyword(s):  
Spinal Cord ◽  
Mitochondrial Dynamics ◽  
Ultrastructural Changes ◽  
In Ovo ◽  
Cytoplasmic Inclusions ◽  
Autophagic Vacuoles ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Mantle Layer ◽  
The Central Nervous System

Methylmercury (MeHg) is a known neurodevelopmental toxicant, which causes changes in various structures of the central nervous system (CNS). However, ultrastructural studies of its effects on the developing CNS are still scarce. Here, we investigated the effect of MeHg on the ultrastructure of the cells in spinal cord layers. Chicken embryos at E3 were treated in ovo with 0.1 μg MeHg/50 μL saline solution and analyzed at E10. Then, we used transmission electron microscopy (TEM) to identify possible damage caused by MeHg to the structures and organelles of the spinal cord cells. After MeHg treatment, we observed, in the spinal cord mantle layer, a significant number of altered mitochondria with external membrane disruptions, crest disorganization, swelling in the mitochondrial matrix, and vacuole formation between the internal and external mitochondrial membranes. We also observed dilations in the Golgi complex and endoplasmic reticulum cisterns and the appearance of myelin-like cytoplasmic inclusions. We observed no difference in the total mitochondria number between the control and MeHg-treated groups. However, the MeHg-treated embryos showed an increased number of altered mitochondria and a decreased number of mitochondrial fusion profiles. Additionally, unusual mitochondrial shapes were found in MeHg-treated embryos as well as autophagic vacuoles similar to mitophagic profiles. In addition, we observed autophagic vacuoles with amorphous, homogeneous, and electron-dense contents, similar to the autophagy. Our results showed, for the first time, the neurotoxic effect of MeHg on the ultrastructure of the developing spinal cord. Using TEM we demonstrate that changes in the endomembrane system, mitochondrial damage, disturbance in mitochondrial dynamics, and increase in mitophagy were caused by MeHg exposure.

Scanning and Transmission Electron Microscopy of Endomentrium in Women Wearing TCu-380-A IUD 's

1977 ◽  
Vol 35 ◽  
pp. 666-667
Author(s):  
A. Gonzalez Angulo ◽  
R. Berlioz ◽  
R. Aznar
Keyword(s):  
Copper Wire ◽  
Uterine Cavity ◽  
Morphological Evidence ◽  
Secretory Phase ◽  
Ultrastructural Studies ◽  
Proliferative Phase ◽  
Contraceptive Devices ◽  
Transmission Electron ◽  
Intrauterine Contraceptive ◽  
Myelin Figure

Recent ultrastructural studies on endometrial tissues from women wearing copper, wire intrauterine devices have disclosed morphological evidence of impaired glycogen degradation and secretion resulting in interference with the viability of blastocysts. Reduced microapocrine secretion observed with the scanning electron microscope supports this (1). In addition, organelle modifications have been observed in the epithelial cells of these women. The changes are seen in biopsies taken in the proliferative phase of the cycle and consist of mitochondrial vacuolation and myelin figure formation. These modifications disappear in the secretory phase and therefore have been regarded as reversible (2).The aim of the present studies was to investigate surface epithelial changes as well as organelle modifications in relation to the site of contact with an IUD that releases greater amounts of copper. Endometrial tissue was obtained from the uterine cavity of four young women wearing TCu-380-A intrauterine contraceptive devices for 4-6 weeks.

Ultrastructural changes in murine lymphoma cells exposed to ultraviolet-A radiation

1991 ◽  
Vol 49 ◽  
pp. 104-105
Author(s):  
Delma P. Thomas ◽  
Dianne E. Godar
Keyword(s):  
Medical Devices ◽  
Blood Cells ◽  
White Blood Cells ◽  
Consumer Products ◽  
Ultrastructural Changes ◽  
Ultraviolet A ◽  
Uv Spectrum ◽  
Lymphoma Cells ◽  
Murine Lymphoma ◽  
Transmission Electron

Ultraviolet radiation (UVR) from all three waveband regions of the UV spectrum, UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm), can be emitted by some medical devices and consumer products. Sunlamps can expose the blood to a considerable amount of UVR, particularly UVA and/or UVB. The percent transmission of each waveband through the epidermis to the dermis, which contains blood, increases in the order of increasing wavelength: UVC (10%) < UVB (20%) < UVA (30%). To investigate the effects of UVR on white blood cells, we chose transmission electron microscopy to examine the ultrastructure changes in L5178Y-R murine lymphoma cells.

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