Image processing for electron energy loss microanalysis
Over the years biology and materials science have profited greatly from the imaging potential of electron microscopy for structural and morphological analysis. Gross structures, grains, subgrains or subcellular organelles, mitochondria and the Golgi were fairly easy to interpret at a glance. However, as more high resolution detail was sought in the form of macromolecular structure, or dislocations or even atomic images, the wave nature, the finite number of electrons and specimen destruction from the high energy of the latter all made interpretation of the images more difficult. Image simulation, image analysis and image processing have helped greatly to extract relevant information and have assisted in our understanding not only of the structure per se but also of the interaction between the structure and the probing electron.