scholarly journals Simulated detection of syndromic classical swine fever on a Finnish pig-breeding farm

2006 ◽  
Vol 135 (2) ◽  
pp. 218-227 ◽  
Author(s):  
S. M. RAULO ◽  
T. LYYTIKÄINEN
Keyword(s):  
Early Detection ◽  
Infectious Diseases ◽  
Health Problem ◽  
General Health ◽  
Rapid Detection ◽  
Classical Swine Fever ◽  
Detection Time ◽  
Infected Farm ◽  
Pig Farms

Although Finland has not experienced a classical swine fever (CSF) epidemic since 1917, the concern about early detection is relevant. The time until detection of CSF on a pig-breeding farm was predicted by simulation, and earlier detection of CSF-infected farms was assessed. Eight to 12 weeks will pass before CSF is detected on a Finnish pig-breeding farm, which resembles detection of the index farm for actual CSF epidemics in Europe. Although notification of suspected CSF on the infected farm accelerates detection the most, interventions aimed at promoting investigations of the general health problem noticed on the farm, or a more comprehensive testing of samples currently arriving from pig farms to the investigating laboratory could shorten detection time by 3 weeks. Results are applicable for further simulation of an event of a CSF epidemic in Finland, and for studying contingency options to promote more rapid detection of infectious diseases of swine not found at present in the country.

scholarly journals Surveillance results of communicable swine diseases in breeding pig farms near Ulaanbaatar city

2020 ◽  
Vol 29 (1) ◽  
pp. 56-63
Author(s):  
Bayarmaa Sukhbaatar ◽  
Amartuvshin Tsolmon ◽  
Shurenchimeg Badamjav ◽  
Gantumur Tsedee ◽  
Erdenebat Bulgan ◽  
...  
Keyword(s):  
Infectious Diseases ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Elisa Test ◽  
Economic Losses ◽  
Respiratory Syndrome Virus ◽  
Serum Samples ◽  
Prophylactic Measures ◽  
Pig Farms ◽  
Swine Diseases

We have done laboratory-based analyses of highly contagious and infectious swine diseases commonly occurring in household and pig farms on 200 swine serum samples collected from Bayanzurkh, Songinokhairkhan, Nalaikh, Khan-Uul district of Ulaanbaatar city, to establish the rate of infection, to gather information on farming and bio-security, to further detect this infectious diseases, for the purpose of risk assessment and preventive research.  According to ELISA test results Classical swine fever 27 (13.5%), Parvovirus 50 (25%), Influenza 16 (8%), Circovirus 112 (56%) samples had shown positive results. Presence of classical swine fever antibody in 27/200 samples are reaction of vaccine immunization.  Also antibody presence of Swine parvovirus, Influenza, circovirus in samples indicates necessity of make it clear that whether symptomless cases around farms.  But antibody absence of porcine food and mouth disease, porcine reproductive and respiratory syndrome virus, swine mycoplasmosis transmissible gastro-enteritis, coronovirus, leptospirosis, brucellosis indicates such disease free in farms. Antibody absence of African swine fever done by surveillance this time shows no further infection however it was occurred in Mongolia early in 2019.  Fundamental surveying is a key tool for monitoring health status of pig farm and formulating eradication program for swine infectious diseases as well as help farmers prevent economic losses by implementing prophylactic measures against sudden cases of such diseases. Thus we suggest that above mentioned surveillance study need to be continued as a tool for monitoring because of it is importance of keep pig farmers sustainable, furthermore crucial for nation`s economy. Улаанбаатар хотын ойролцоо үржүүлж буй гахайд зонхилон тохиолдох халдварт өвчнүүдийг тандан судалсан дүн Улаанбаатар хотын Баянзүрх, Налайх, Сонгинохайрхан, Хан-Уул зэрэг 4 дүүргийн өрхийн болон аж ахуйн гахайд зонхилон тохиолддог гоц болон халдварт өвчнүүдийн лабораторид суурилсан шинжилгээг хийх, халдварлалтын түвшинг тогтоох, аж ахуй эрхлэлт, биоаюулгүй байдлын талаар мэдээлэл цуглуулах, цаашид халдварт өвчнийг эрт илрүүлэх, эрсдэлийг үнэлэх, урьдчилан сэргийлэх судалгааны ажлын зорилгын хүрээнд нийт 200 дээжинд эсрэгбием илрүүлэх ЭЛИЗА урвалаар шинжилгээг хийхэд гахайн сонгомол мялзан 27 (13.5%), парвовирус 50 (25%), томуу 16 (8%), цирковирус 112 (56%) дээж эерэг дүн үзүүлэв. Гахайн сонгомол мялзан өвчний эсрэгбием (27/200) илэрсэн нь урьдчилан сэргийлэх зорилгоор тарьсан вакцины урвал юм. Гахайн парвовирус, гахайн томуу, гахайн цирковирус зэрэг өвчний вирусын эсрэгбием илэрч байгаа нь ил шинж тэмдэггүй, халдварын тархалт байгаа эсэхийг баталгаажуулах шаардлагатайг харуулж байна. Харин гахайн шүлхий, микоплазм, гахайн үржил, амьсгалын замын хам шинж, гахайн ходоод гэдэсний замын халдварт үрэвсэл, коронавирус, лептоспироз, бруцеллёз зэрэг өвчний эсрэгбием илрээгүй нь халдваргүйг харуулж байна. Гахайн африкийн мялзан өвчин Монгол оронд 2019 оны эхээр гарч байсан ч энэ удаагийн тандалтаар эсрэгбием илрээгүй нь өвчлөл дахиж гараагүй болохыг харуулж байна. Тандан судалгаа нь гахайн эрүүл мэндийг хянах, эрүүлжүүлэх хөтөлбөрийг боловсруулах гахайн аж ахуйд тохиолдох эдийн засгийн болзошгүй эрсдэлээс урьдчилан хянахад чухал ач холбогдолтой.  Тиймээс энэхүү суурь судалгааг жил бүр тогтмол хийх нь гахайн аж ахуйг тогтвортой байлгах төдийгүй улс орны эдийн засагт чухал ач холбогдолтой тул гахайн эрүүл мэндийг хянах зорилгоор үргэлжлүүлэн хийж байх шаардлагатай байна. Түлхүүр үг: Гахайн халдварт өвчнүүд, ЭЛИЗА, тандан судалгаа, эсрэгбием

scholarly journals A Critical Review about Different Vaccines against Classical Swine Fever Virus and Their Repercussions in Endemic Regions

Vaccines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 154
Author(s):  
Liani Coronado ◽  
Carmen L. Perera ◽  
Liliam Rios ◽  
María T. Frías ◽  
Lester J. Pérez
Keyword(s):  
Infectious Diseases ◽  
Classical Swine Fever Virus ◽  
Critical Review ◽  
Global Economy ◽  
Scientific Community ◽  
Ethical Issues ◽  
Control Measure ◽  
Classical Swine Fever ◽  
Current Review ◽  
Available Information

Classical swine fever (CSF) is, without any doubt, one of the most devasting viral infectious diseases affecting the members of Suidae family, which causes a severe impact on the global economy. The reemergence of CSF virus (CSFV) in several countries in America, Asia, and sporadic outbreaks in Europe, sheds light about the serious concern that a potential global reemergence of this disease represents. The negative aspects related with the application of mass stamping out policies, including elevated costs and ethical issues, point out vaccination as the main control measure against future outbreaks. Hence, it is imperative for the scientific community to continue with the active investigations for more effective vaccines against CSFV. The current review pursues to gather all the available information about the vaccines in use or under developing stages against CSFV. From the perspective concerning the evolutionary viral process, this review also discusses the current problematic in CSF-endemic countries.

scholarly journals A socio-psychological investigation into limitations and incentives concerning reporting a clinically suspect situation aimed at improving early detection of classical swine fever outbreaks

2010 ◽  
Vol 142 (1-2) ◽  
pp. 108-118 ◽  
Author(s):  
A.R.W. Elbers ◽  
M.J. Gorgievski-Duijvesteijn ◽  
P.G. van der Velden ◽  
W.L.A. Loeffen ◽  
K. Zarafshani
Keyword(s):  
Early Detection ◽  
Classical Swine Fever

scholarly journals Mammalian Cell-Based Immunoassay for Detection of Viable Bacterial Pathogens

2020 ◽  
Vol 11 ◽  
Author(s):  
Luping Xu ◽  
Xingjian Bai ◽  
Shivendra Tenguria ◽  
Yi Liu ◽  
Rishi Drolia ◽  
...  
Keyword(s):  
Shelf Life ◽  
Mammalian Cell ◽  
Rapid Detection ◽  
Mammalian Cells ◽  
Host Cells ◽  
Detection Time ◽  
Primary Methods ◽  
Widespread Application ◽  
Lateral Flow Assays ◽  
Set Up

Rapid detection of live pathogens is of paramount importance to ensure food safety. At present, nucleic acid-based polymerase chain reaction and antibody-based lateral flow assays are the primary methods of choice for rapid detection, but these are prone to interference from inhibitors, and resident microbes. Moreover, the positive results may neither assure virulence potential nor viability of the analyte. In contrast, the mammalian cell-based assay detects pathogen interaction with the host cells and is responsive to only live pathogens, but the short shelf-life of the mammalian cells is the major impediment for its widespread application. An innovative approach to prolong the shelf-life of mammalian cells by using formalin was undertaken. Formalin (4% formaldehyde)-fixed human ileocecal adenocarcinoma cell line, HCT-8 on 24-well tissue culture plates was used for the capture of viable pathogens while an antibody was used for specific detection. The specificity of the Mammalian Cell-based ImmunoAssay (MaCIA) was validated with Salmonella enterica serovar Enteritidis and Typhimurium as model pathogens and further confirmed against a panel of 15 S. Enteritidis strains, 8 S. Typhimurium, 11 other Salmonella serovars, and 14 non-Salmonella spp. The total detection time (sample-to-result) of MaCIA with artificially inoculated ground chicken, eggs, milk, and cake mix at 1–10 CFU/25 g was 16–21 h using a traditional enrichment set up but the detection time was shortened to 10–12 h using direct on-cell (MaCIA) enrichment. Formalin-fixed stable cell monolayers in MaCIA provide longer shelf-life (at least 14 weeks) for possible point-of-need deployment and multi-sample testing on a single plate.

Rapid Detection of Listeria in Ice Cream in 13 Hours Using the Roka Listeria Detection Assay

2018 ◽  
Vol 101 (6) ◽  
pp. 1806-1812
Author(s):  
Elba Veronica Arias-Rios ◽  
Kristina Tenney ◽  
Tam Mai ◽  
Sam Anderson ◽  
Ruth Marie Cantera ◽  
...  
Keyword(s):  
Early Detection ◽  
New Media ◽  
Rapid Detection ◽  
Method Comparison ◽  
Reference Method ◽  
Ice Cream ◽  
Detection Methods ◽  
Media Formulation ◽  
Detection Assay ◽  
Highly Sensitive

Abstract Background: Listeria contamination is a major concern in the ice cream industry; therefore, early and accurate detection is vital. Current detection methods require about a 24 h enrichment period for detection. Objective: Enhance the early detection of Listeria in ice cream using the highly sensitive isothermal ribosomal RNA-based Roka/Atlas Listeria Detection Assay. Methods: The R2 Medium was developed for Listeria enrichment by Molecular Epidemiology, Inc. (Seattle, WA). Comparative growth curve studies were performed on the new R2 Medium for Listeria and the currently validated media for the Roka Listeria Detection Assay. Subsequently, a method comparison between the Roka Listeria Detection Assay and the U.S. Food and Drug Administration’s (FDA) Bacteriological Analytical Manual (BAM) Chapter 10 reference method on ice cream was carried out. Results: The R2 Medium supports the growth of L. monocytogenes better than Buffered Listeria Enrichment Broth, Demi-Fraser broth, and Modified University of Vermont Broth, as indicated by the faster growth rate of the organism. When used as an enrichment medium in a method comparison study of ice cream, the results showed that R2 Medium–enriched samples tested with the Roka Listeria Detection Assay gave an equivalent performance compared with the 24 h FDA-BAM reference method at 10 and 18 h post-enrichment for Listeria. Conclusions: The results from this study indicate that the new R2 Medium and the highly sensitive Roka Listeria Detection Assay allowed for the rapid detection of Listeria species in ice cream in 13 h. Highlights: The Roka Listeria Detection Assay, in conjunction with a new media formulation (R2 Medium), allowed for the early detection of Listeria in ice cream and may be applied in other food matrixes and environmental samples.

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