Corrosion Cast Demonstration of Tongue Vasculature of Normal Wistar Rats

1998 ◽  
Vol 4 (S2) ◽  
pp. 920-921
Author(s):  
M. C. K. Makiyama ◽  
I. Watanabe ◽  
R.P. Chopard ◽  
M. M. Iyomasa ◽  
C. I. Mizusaki ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Three Dimensional ◽  
Vascular Network ◽  
Casting Method ◽  
Sem Images ◽  
Lingual Mucosa ◽  
Methacrylate Monomer ◽  
Corrosion Cast ◽  
Methyl Methacrylate Monomer ◽  
Scanning Electron

The purpose of this study has been to undertake a three-dimensional analysis of the vascular network of the lamina propria in the lingual mucosa of the rat. Using the corrosive-resin casting method, casts of the vascular network were prepared and examined by scanning electron microscopy. We used 21th day normal wistar rats. They were anesthetized and perfused with saline solution and after with mercox (25ml of resin, 5ml of methyl methacrylate monomer). Then, the tongues were resected and placed in warm water bath at 60° C for 4 h. The tongue epithelium were digested with 20% NaOH for 4 days. The vascular resin casts washed in distilled water, several times. The dried vascular casts were mounted on the stubs with conductive paste, coated with gold and examined with a JEOL JSM- 6100 scanning electron microscope. In the SEM images, the lingual mucosa revealed arteriolae which divide into smaller branches entering into connective tissue papillae.

Some Simple Quantitative Applications of Vascular Corrosion Casting

1998 ◽  
Vol 4 (S2) ◽  
pp. 914-915
Author(s):  
Dr. Fred E. Hossler
Keyword(s):  
Methyl Methacrylate ◽  
Ringer Solution ◽  
Corrosion Casting ◽  
Corrosion Casts ◽  
3 Dimensional ◽  
Quantitative Measurements ◽  
Methacrylate Monomer ◽  
Corrosion Cast ◽  
Methyl Methacrylate Monomer

The primary use of vascular corrosion casting has been and likely will continue to be for describing the 3-dimensional anatomy of the microvasculature of organs and tissues. In these studies the assumption is made that the corrosion cast faithfully replicates the anatomy, dimensions, and distribution of the vasculature of these tissues and organs. If this assumption is accurate, and it appears to be nearly so in tissues where measurements have been made, it follows that the casts should lend themselves to quantitative measurements. I summarize here some simple quantitative measurements we have made from corrosion casts. For these studies, cannulas were inserted into arteries leading to the organ of interest in anesthetized animals, and the blood was removed by flushing with Ringer solution or buffered saline, at physiological temperatures and pressures. Resin consisting of Mercox (or Mercox diluted with methyl methacrylate monomer) and catalyst was infused via the same cannula until the onset of polymerization.

Graphene-Poly(Methyl Methacrylate) Composites Prepared by Two Methods

2011 ◽  
Vol 335-336 ◽  
pp. 49-53 ◽  
Author(s):  
Lin Lei ◽  
Jian Hui Qiu ◽  
Xue Li Wu ◽  
Yang Zhao ◽  
Eiichi Sakai
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Methyl Methacrylate ◽  
Graphite Oxide ◽  
Graphene Nanosheet ◽  
Poly Methyl Methacrylate ◽  
Transmission Electron ◽  
Methacrylate Monomer ◽  
Methyl Methacrylate Monomer ◽  
Scanning Electron

Graphene-poly(methyl methacrylate) (GNS-PMMA) composites were prepared by two methods. Graphite oxide nanosheet-poly(methyl methacrylate) (GO-PMMA) composites were prepared of methyl methacrylate monomer and the presence of graphite oxide (GO). Then the GO-PMMA composites were reduced to graphene nanosheet-poly(methyl methacrylate) by using hydrazine hydrate. The obtained composites were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and Transmission electron microscopy (TEM). The composite exhibited conductivities in the 1.58×10-9-4.21 S/cm range, depending on the amounts of graphite oxide and PMMA.

Three-dimensional aspects of the structural characteristics and kidney angioarchitecture of adult and aged wistar rats: A scanning electron microscopy study

2013 ◽  
Vol 76 (5) ◽  
pp. 538-544 ◽  
Author(s):  
Cristina De Sousa Bolina ◽  
Regina De Sousa Bolina-Matos ◽  
Paulo Henrique De Matos Alves ◽  
Diego Pulzato Cury ◽  
Adriano Polican Ciena ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wistar Rats ◽  
Structural Characteristics ◽  
Three Dimensional ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Scanning Electron Microscopy Study ◽  
Scanning Electron

scholarly journals Three-Dimensional Architecture of Cerebral Microvessels with a Scanning Electron Microscope: A Cerebrovascular Casting Method for Fetal and Adult Rats

1984 ◽  
Vol 4 (2) ◽  
pp. 290-296 ◽  
Author(s):  
Yasuji Yoshida ◽  
Fusahiro Ikuta
Keyword(s):  
Three Dimensional ◽  
Brain Slices ◽  
Sodium Hypochlorite Solution ◽  
Casting Method ◽  
Electron Microscopic ◽  
Adult Rats ◽  
Cerebral Microvessels ◽  
Vascular Casts ◽  
The Brain ◽  
Scanning Electron

Vascular casting for scanning electron microscopic studies on microvascular architecture is in common use for various visceral organs in the field of anatomy. However, only a few studies have been performed on the brain using the previously reported casting method, and no detailed descriptions deal with suitable methodology for producing brain vascular casts. Our casting method, introduced here, for the CNS from the fetal to the adult stage involves the following modifications: (1) Perfusion fixation of the brain is carried out before injecting the plastic resin for casting into the cerebral blood vessels; (2) digestion of nervous tissue is accomplished with a sodium hydroxide and sodium hypochlorite solution; and (3) vascular casts are dried by a freeze-drying method, while the nondigested brain slices opposite the casts can be investigated with light microscopy and transmission electron microscopy. This modified casting method enables one to represent the microvascular system of the rat brain three-dimensionally from embryonal day 17 onward. It is hoped that this method will prove to be a useful tool in morphological vascular research on the nervous system.

scholarly journals A Concept for Three-Dimensional Particle Metrology Based on Scanning Electron Microscopy and Structure-from-Motion Photogrammetry

2020 ◽  
Vol 125 ◽  
Author(s):  
Vipin N. Tondare
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Structure From Motion ◽  
3D Model ◽  
Three Dimensional ◽  
Sem Images ◽  
Shape Information ◽  
Size And Shape ◽  
Future Work ◽  
Scanning Electron

Scanning electron microscopy (SEM) has been frequently used for size and shape measurements of particles. SEM images offer two-dimensional (2D) information about a particle’s lateral dimensions. Unfortunately, information about the particle’s three-dimensional (3D) size and shape remains unavailable. To resolve this issue, I propose a new concept in SEM: 3D particle metrology obtained by applying structure-from-motion (SfM) algorithms to multiple rotational SEM images of particles deposited onto a cylindrical substrate to generate a 3D model from which size and shape information can be extracted. Particles can have any size that is suitable for SEM imaging. SEM images of the sample can be acquired from 0° to 360° using a rotational-tip SEM substage. Here, I will discuss the concept and, for clarity, illustrate it with aquarium gravel particles that are glued onto a craft roll and imaged optically before generating the 3D model of that handmade craft. Future work will include the experimental SEM realization, as well as further development of the SfM algorithms. In my view, this proposed concept may become an integral part of SEM-based particle metrology.

scholarly journals Quantitative 3D Reconstruction from Scanning Electron Microscope Images Based on Affine Camera Models

Sensors ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 3598 ◽  
Author(s):  
Stefan Töberg ◽  
Eduard Reithmeier
Keyword(s):  
Laser Scanning ◽  
Three Dimensional ◽  
Confocal Laser ◽  
Stereo Pair ◽  
Sem Images ◽  
Self Calibration ◽  
Dense Matching ◽  
Electron Microscopes ◽  
Affine Camera ◽  
Scanning Electron

Scanning electron microscopes (SEMs) are versatile imaging devices for the micro- and nanoscale that find application in various disciplines such as the characterization of biological, mineral or mechanical specimen. Even though the specimen’s two-dimensional (2D) properties are provided by the acquired images, detailed morphological characterizations require knowledge about the three-dimensional (3D) surface structure. To overcome this limitation, a reconstruction routine is presented that allows the quantitative depth reconstruction from SEM image sequences. Based on the SEM’s imaging properties that can be well described by an affine camera, the proposed algorithms rely on the use of affine epipolar geometry, self-calibration via factorization and triangulation from dense correspondences. To yield the highest robustness and accuracy, different sub-models of the affine camera are applied to the SEM images and the obtained results are directly compared to confocal laser scanning microscope (CLSM) measurements to identify the ideal parametrization and underlying algorithms. To solve the rectification problem for stereo-pair images of an affine camera so that dense matching algorithms can be applied, existing approaches are adapted and extended to further enhance the yielded results. The evaluations of this study allow to specify the applicability of the affine camera models to SEM images and what accuracies can be expected for reconstruction routines based on self-calibration and dense matching algorithms.

Description of Ore Particles from X-Ray Microtomography (XMT) Images, Supported by Scanning Electron Microscope (SEM)-Based Image Analysis

2018 ◽  
Vol 24 (5) ◽  
pp. 461-470 ◽  
Author(s):  
Orkun Furat ◽  
Thomas Leißner ◽  
Ralf Ditscherlein ◽  
Ondřej Šedivý ◽  
Matthias Weber ◽  
...  
Keyword(s):  
Image Analysis ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Three Dimensional ◽  
Mineralogical Composition ◽  
Particle Systems ◽  
3D Image ◽  
Sem Images ◽  
X Ray ◽  
Scanning Electron

AbstractIn this paper, three-dimensional (3D) image data of ore particle systems is investigated. By combining X-ray microtomography with scanning electron microscope (SEM)-based image analysis, additional information about the mineralogical composition from certain planar sections can be gained. For the analysis of tomographic images of particle systems the extraction of single particles is essential. This is performed with a marker-based watershed algorithm and a post-processing step utilizing a neural network to reduce oversegmentation. The results are validated by comparing the 3D particle-wise segmentation empirically with 2D SEM images, which have been obtained with a different imaging process and segmentation algorithm. Finally, a stereological application is shown, in which planar SEM images are embedded into the tomographic 3D image. This allows the estimation of local X-ray attenuation coefficients, which are material-specific quantities, in the entire tomographic image.

scholarly journals Three-Dimensional (3D) Nanometrology Based on Scanning Electron Microscope (SEM) Stereophotogrammetry

2017 ◽  
Vol 23 (5) ◽  
pp. 967-977 ◽  
Author(s):  
Vipin N. Tondare ◽  
John S. Villarrubia ◽  
András E. Vladár
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Three Dimensional ◽  
Sample Surface ◽  
3D Models ◽  
Sem Images ◽  
Low Contrast ◽  
Software Packages ◽  
Virtual Samples ◽  
Scanning Electron

AbstractThree-dimensional (3D) reconstruction of a sample surface from scanning electron microscope (SEM) images taken at two perspectives has been known for decades. Nowadays, there exist several commercially available stereophotogrammetry software packages. For testing these software packages, in this study we used Monte Carlo simulated SEM images of virtual samples. A virtual sample is a model in a computer, and its true dimensions are known exactly, which is impossible for real SEM samples due to measurement uncertainty. The simulated SEM images can be used for algorithm testing, development, and validation. We tested two stereophotogrammetry software packages and compared their reconstructed 3D models with the known geometry of the virtual samples used to create the simulated SEM images. Both packages performed relatively well with simulated SEM images of a sample with a rough surface. However, in a sample containing nearly uniform and therefore low-contrast zones, the height reconstruction error was ≈46%. The present stereophotogrammetry software packages need further improvement before they can be used reliably with SEM images with uniform zones.

Imaging and Volumetric Quantitation of Vascular Corrosion Casts with Laser Scanning Confocal Microscopy.

2000 ◽  
Vol 6 (S2) ◽  
pp. 562-563 ◽  
Author(s):  
K. Czymmek ◽  
R. C. Wagner ◽  
F. E. Hossler ◽  
R. Kao
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Quantitative Information ◽  
Laser Scanning Confocal Microscopy ◽  
Hot Water ◽  
Corrosion Casts ◽  
Sem Images ◽  
Corrosion Cast ◽  
Vascular Corrosion Casts

Vascular corrosion casts provide faithful replicas of the three-dimensional anatomy of blood vessel system of organs and tissues. In addition, corrosion casts can be used to obtain quantitative information regarding vessel and tissue spaces. However, morphometric measurements of SEM images of corrosion casts is difficult due to severe specimen tilt, parallax and the inability to image 3D casts from all angles. We present evidence that confocal microscopy can be used to collect all of the 3D information of a corrosion cast in a z-series of optical slices. Surface and volumetric parameters of casted and non-casted space are readily retrievable from a stack of optical sections through a corrosion cast.Various tissues and organs were exsanguinated with heparenized saline following cannulization of regional arteries and casts were made by infusing Mercox resin or Mercox diluted with 20% methyl methacrylate monomer. Tissues were macerated with alternating rinses in 5% KOH and hot water.

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