Comparative Analysis of Root Dentin Morphology and Structure of Human Versus Bovine Primary Teeth

2015 ◽  
Vol 21 (3) ◽  
pp. 689-694 ◽  
Author(s):  
Bruna M. Costa ◽  
Alexsandra S. Iwamoto ◽  
Regina M. Puppin-Rontani ◽  
Fernanda M. Pascon
Keyword(s):  
Repeated Measures ◽  
Primary Teeth ◽  
Primary Root ◽  
Repeated Measures Anova ◽  
Root Canals ◽  
Morphology And Structure ◽  
Root Dentin ◽  
Dentin Tubules ◽  
Morphological Differences ◽  
Scanning Electron

AbstractThis study evaluated the structural and morphological differences between human and bovine primary root canals. Primary human maxillary central incisors (H) (n=9) and primary bovine incisors (B) (n=9) were selected. The roots were sectioned in the vestibular-lingual direction, planed and delimited in cervical, middle, and apical thirds. Tubule density (number of tubules per mm2) and diameter were analyzed by scanning electron microscopy (1,000 and 5,000×) using Image J 1.47 software. Data were submitted to two-way repeated measures ANOVA and Tukey tests (α=0.05). The highest tubule density was observed for B (28.527±1.717 mm2) compared with H (15.931±0.170 mm2) (p<0.01). Regarding root thirds, the cervical third presented a greater tubule density (26.417±11.654 mm2) than the apical third (17.999±5.873 mm2). The diameter of the dentin tubules was not different for cervical (3.50±0.08 µm), middle (3.45±0.30 µm) and apical thirds (3.42±0.33 µm) and substrate (H—3.29±0.14 µm; B—3.63±0.06 µm). It could be concluded that: (1) the radicular dentin structure of human and bovine primary teeth and root thirds differ in terms of the tubule density; (2) the radicular dentin morphology of human and bovine primary teeth and root thirds are similar in terms of the diameter of the dentin tubules.

scholarly journals Microhardness evaluation of root dentin after using resin sealer solvents

2021 ◽  
Vol 15 (4) ◽  
pp. 256-261
Author(s):  
Gamze Nalci ◽  
Tayfun Alaçam ◽  
Bülent Altukaynak
Keyword(s):  
Repeated Measures ◽  
Saline Solution ◽  
Prolonged Exposure ◽  
Repeated Measures Anova ◽  
Ultrasonic Agitation ◽  
Gutta Percha ◽  
Before And After ◽  
Root Dentin ◽  
Substantial Decline ◽  
Hardness Values

Background. This study aimed to assess the effects of methyl ethyl ketone (MEK) and ethyl acetate (EA) on dentin microhardness, used as resin sealer solvents. Methods. Eighty halves of single-rooted teeth were randomly divided into four groups to apply MEK, EA, chloroform, or saline solution. Vickers hardness values were measured for three root levels before and after the direct application of solvents for 5 and 15 minutes or a 1-minute application with ultrasonic agitation. The results were analyzed using repeated-measures ANOVA, and adjustments were made for comparisons with Bonferroni tests. Results. The dentin microhardness decreased in all the solvent groups (P<0.05). The changes in microhardness increased with prolonged exposure times, except for the saline solution group. Chloroform exhibited the most significant decrease in value. Furthermore, ultrasonic agitation elicited a more substantial decline in values. Conclusion. MEK and EA might be preferred over chloroform as a solvent for resin sealers because they offer an attenuated decrease in dentin microhardness and do not have gutta-percha-dissolving properties.

scholarly journals Comparison of root canal length measurement methods in primary teeth

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Mine Koruyucu ◽  
Merve Bayram ◽  
Yelda Kasımoğlu ◽  
Figen Seymen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Root Canal ◽  
Primary Teeth ◽  
Conventional Radiography ◽  
Actual Length ◽  
Root Canals ◽  
Working Length ◽  
Electronic Apex Locator ◽  
Scanning Electron

Objectives: The purpose of this study was to evaluate the accuracy of conventional radiography, intraoral digital radiovisiography and electronic apex locator in determining the working length of root canals in primary teeth (in-vivo) and to compare the results with scanning electron microscopy measurements (ex-vivo). Materials and Methods: This study was conducted on 50 primary molar teeth. Standard endodontic access cavity preparations were prepared and the actual length was calculated by calibrated investigators. Working lengths were determined by using conventional radiography, RVG and apex locator in a total of 116 root canals. After the extraction of the teeth, each canal length was determined by using SEM. Data were analysed statistically by using Oneway Anova, Tukey HDS, Student t test and Bonferroni tests. Results: The mean root length measurements with conventional radiography were significantly higher than apex locator, RVG and SEM. The accuracy of apex locator and RVG were higher than conventional radiography in determining the working length in primary teeth. Conclusions: The electronic apex locators provide an acceptable level of accuracy in determining root canal length in primary teeth. Clinical Relevance: The purpose of this study was to evaluate the accuracy of conventional radiography, intraoral digital radiovisiography and electronic apex locator in determining the working length of root canals in primary teeth and,to compare the results with scanning electron microscopy measurements.

scholarly journals Comparison of Shaping Ability between Continuous Rotary and Manual Instrumentations in Pediatric Endodontics

2021 ◽  
Vol 28 (2) ◽  
Author(s):  
Burcu Güçyetmez Topal ◽  
◽  
Seda Falakaloğlu ◽  
Hatice Türkoğlu ◽  
◽  
...  
Keyword(s):  
Primary Teeth ◽  
Primary Root ◽  
In Vitro Study ◽  
Primary Molars ◽  
Preparation Time ◽  
Root Canals ◽  
Apical Transportation ◽  
Canal Transportation ◽  
Shaping Ability

Objective: The aim of this in vitro study was to evaluate the efficacy of rotary EndoArt Ni-Ti Gold Pedo Kit and K-files in shaping ability, canal transportation, centering ability and instrumentation time in primary molars. Methods: For the study total of 30 extracted primary molars root with minimum 7mm root length were selected. Shaping of root canals in primary molars were done using the two systems, and CBCT and specialized software were used for scanning and analysis of pre-operative and post-operative to evaluate the groups for their shaping properties, apical transportation and preparation time in primary root canals. Results: No differences were found in canal transportation measures and instrumentation time between the two groups (p>0.05). The EndoArt group removed more dentin compared to K-file in all sides of the root curvature. The statistical differences were significant for coronal and middle third of the root (p<0.05). Conclusions: Under the conditions of this study, rotary EndoArt Ni-Ti Gold Pedo Kit provided more conical canals than K-files in primary teeth.

COMPARISON OF MANUAL AND ROTARY INSTRUMENTATION TECHNIQUES FOR REMAINING DENTIN THICKNESS IN PRIMARY TEETH USING CBCT

2019 ◽  
Vol 1 (1) ◽  
pp. 01-06
Author(s):  
ANCHANA V ◽  
SHENVI AKSHATA AJAY ◽  
PRAHLAD GADICHERLA ◽  
DHANANJAYA G ◽  
PUSHPALATHA C
Keyword(s):  
Stainless Steel ◽  
Null Hypothesis ◽  
Primary Teeth ◽  
Primary Root ◽  
Root Canal Preparation ◽  
Cleaning Efficiency ◽  
Root Canals ◽  
Before And After ◽  
3D Software ◽  
Dentin Thickness

Aim: The purpose of this study was to compare the residual dentin thickness after manual and rotary instrumentation technique in primary root canals using CBCT. The null hypothesis was that there is no difference in the cleaning efficiency of both manual and rotary technique. Methodology: Manual filing was performed on 30 root canals using stainless steel S-files. Rotary instrumentation was carried out on a further 30 root canals, using rotary ProTaper files in a 300-rotation per minute (r.p.m.) Handpiece (NSK Endomate DT) according to the manufacturer’s instructions. The roots were aligned perpendicularly to the beam, and they were scanned before and after instrumentation by using the CBCT operating at 60 kV and 2.5mA. Pre-instrumentation and post-instrumentation measurements of canals were performed by using the Carestream 3D software. Results: When both the manual and rotary methods of canal instrumentation were compared, it was found that the reduction of dentinal thickness with respect to the manual method was more than the rotary method. Conclusions: There was more dentine thickness reduction in manual instrumentation than rotary instrumentation for root canal preparation in primary teeth.

scholarly journals Microbial Reduction by Two Chemical-Mechanical Protocols in Primary Teeth with Pulp Necrosis and Periradicular Lesion - An In Vivo Study

2014 ◽  
Vol 25 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Thaisa Cezária Triches ◽  
Luciene Cristina de Figueiredo ◽  
Magda Feres ◽  
Sérgio Fernando Torres de Freitas ◽  
Gláucia Santos Zimmermann ◽  
...  
Keyword(s):  
Primary Teeth ◽  
Primary Root ◽  
Microbial Reduction ◽  
Treatment Groups ◽  
Single Visit ◽  
Root Canals ◽  
Endodontic Infection ◽  
Multiple Visit ◽  
Before And After

The objective of this study was to determine the efficacy of chemical-mechanical procedures of two endodontic protocols for septic content reduction of root canals from primary teeth with pulp necrosis and periradicular lesion. Twenty-four primary root canals with pulp necrosis and periradicular lesion were divided into two treatment groups (n=12): multiple-visit and single-visit protocols. Samples were collected using sterile paper points before and after endodontic cleaning followed by microbiological identification through checkerboard DNA-DNA hybridization. Statistical analysis was performed using Proportion Test for score=0 comparing the findings before and after treatment for each group (Wilcoxon's test) as well as the differences in scores between protocols (Mann-Whitney's test) (p<0.05). Data were expressed as prevalence (presence or absence) and estimate of the average count (x105 cells) of each species. Differences in proportions of score=0 prior to treatment were non-significant (p=0.415), demonstrating equivalence between groups. A significant increase in score=0 was detected after treatment for both groups (p<0.0001). Single-visit protocol achieved a significantly greater reduction in mean scoring following endodontic treatment (p=0.024). Both protocols were capable of significantly reducing septic content in root canals of primary teeth with periradicular lesion. Moreover, single-visit protocol showed greater efficacy in reducing endodontic infection.

A scanning electron microscopic study of the eggs of Culicoides variipennis (Diptera: Ceratopogonidae)

1987 ◽  
Vol 45 ◽  
pp. 884-885 ◽  
Author(s):  
R. A. Nunamaker ◽  
C. E. Nunamaker ◽  
B. C. Wick
Keyword(s):  
Distinct Species ◽  
Hemorrhagic Disease ◽  
Insect Vector ◽  
Electron Microscopic ◽  
Important Species ◽  
Scanning Electron Microscopic Study ◽  
Laboratory Colony ◽  
Culicoides Variipennis ◽  
Morphological Differences ◽  
Scanning Electron

Culicoides variipennis (Coquillett) is probably the most economically important species of biting midge in the U.S. due to its involvement in the transmission of bluetongue (BT) disease of sheep, cattle and ruminant wildlife, and epizootic hemorrhagic disease (EHD) of deer. Proposals have been made to recognize the eastern and western populations of this insect vector as distinct species. Others recommend use of the term “variipennis complex” until such time that the necessary biosystematic studies have been made to determine the genetic nature and/or minute morphological differences within the population structure over the entire geographic range of the species. Increasingly, students of ootaxonomy are relying on scanning electron microscopy (SEM) to assess chorionic features. This study was undertaken to provide comparative chorionic data for the C. variipennis complex.Culicoides variipennis eggs were collected from a laboratory colony maintained in Laramie, Wyoming.

One Size Fits All?

Methodology ◽  
2012 ◽  
Vol 8 (1) ◽  
pp. 23-38 ◽  
Author(s):  
Manuel C. Voelkle ◽  
Patrick E. McKnight
Keyword(s):  
Repeated Measures ◽  
Structural Equation ◽  
Type I Error ◽  
Equation Modeling ◽  
Type I ◽  
Finite Sample ◽  
Traditional Methods ◽  
Repeated Measures Anova ◽  
Special Cases ◽  
Latent Curve

The use of latent curve models (LCMs) has increased almost exponentially during the last decade. Oftentimes, researchers regard LCM as a “new” method to analyze change with little attention paid to the fact that the technique was originally introduced as an “alternative to standard repeated measures ANOVA and first-order auto-regressive methods” (Meredith & Tisak, 1990, p. 107). In the first part of the paper, this close relationship is reviewed, and it is demonstrated how “traditional” methods, such as the repeated measures ANOVA, and MANOVA, can be formulated as LCMs. Given that latent curve modeling is essentially a large-sample technique, compared to “traditional” finite-sample approaches, the second part of the paper addresses the question to what degree the more flexible LCMs can actually replace some of the older tests by means of a Monte-Carlo simulation. In addition, a structural equation modeling alternative to Mauchly’s (1940) test of sphericity is explored. Although “traditional” methods may be expressed as special cases of more general LCMs, we found the equivalence holds only asymptotically. For practical purposes, however, no approach always outperformed the other alternatives in terms of power and type I error, so the best method to be used depends on the situation. We provide detailed recommendations of when to use which method.

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