The use of extracted lignin from steam-treated wheat straw to protect protein from rumen microbial degradation

2000 ◽  
Vol 2000 ◽  
pp. 49-49
Author(s):  
M. Zahedifar ◽  
F.B. Castro ◽  
E.R. Ørskov
Keyword(s):  
Molecular Weight ◽  
Phenolic Compounds ◽  
Wheat Straw ◽  
Microbial Degradation ◽  
Lower Molecular Weight ◽  
Steam Treatment ◽  
Hydroxyl Groups ◽  
Lignocellulosic Materials ◽  
Positive Effect

During steam treatment of lignocellulosic materials lignin is depolymerised (Zahedifar, 1996) to lower molecular weight phenolic compounds. Those phenolics may have protein precipitating capacity (PPC) due to bearing some hydroxyl groups on their molecule (Kawamoto et al, 1992) as do some other phenolics like tannins. Protein precipitating capacity of tannins may have a positive effect in ruminants by protecting proteins from microbial degradation in the rumen. The aim of this study was to assess the PPC of the phenolic compounds extracted from steam-treated wheat straw (STWS) and possible use of them to protect protein from rumen microbial degradation.

scholarly journals The Effect of Ball Milling Time on the Isolation of Lignin in the Cell Wall of Different Biomass

2021 ◽  
Vol 9 ◽  
Author(s):  
Guangrong Yang ◽  
Xueying An ◽  
Shilong Yang
Keyword(s):  
Molecular Weight ◽  
Cell Wall ◽  
Optimum Condition ◽  
Ball Milling ◽  
Milling Time ◽  
Lower Molecular Weight ◽  
Hydroxyl Groups ◽  
Milled Wood Lignin ◽  
Ball Milling Time ◽  
Poplar Sawdust

Ball milling technology is the classical technology to isolate representative lignin in the cell wall of biomass for further investigation. In this work, different ball milling times were carried out on hardwood (poplar sawdust), softwood (larch sawdust), and gramineous material (bamboo residues) to understand the optimum condition to isolate the representative milled wood lignin (MWL) in these different biomass species. Results showed that prolonging ball milling time from 3 to 7 h obviously increased the isolation yields of MWL in bamboo residues (from 39.2% to 53.9%) and poplar sawdust (from 15.5% to 35.6%), while only a slight increase was found for the MWL yield of larch sawdust (from 23.4% to 25.8%). Importantly, the lignin substructure of ß-O-4 in the MWL samples from different biomasses can be a little degraded with the increasing ball milling time, resulting in the prepared MWL with lower molecular weight and higher content of hydroxyl groups. Based on the isolation yield and structure features, milling time with 3 and 7 h were sufficient to isolate the representative lignin (with yield over 30%) in the cell wall of bamboo residues and poplar sawdust, respectively, while more than 7 h should be carried out to isolate the representative lignin in larch sawdust.

scholarly journals The Application of Ultrasound and Enzymes Could Be Promising Tools for Recovering Polyphenols during the Aging on Lees Process in Red Winemaking

Foods ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 19
Author(s):  
Andrea Osete-Alcaraz ◽  
Ana Belén Bautista-Ortín ◽  
Paula Pérez-Porras ◽  
Encarna Gómez-Plaza
Keyword(s):  
Molecular Weight ◽  
Phenolic Compounds ◽  
Cell Walls ◽  
Final Concentration ◽  
Low Molecular Weight ◽  
Acylated Anthocyanins ◽  
The Individual ◽  
Positive Effect ◽  
Tannin Extraction ◽  
Pectolytic Enzyme

The final concentration of phenolic compounds in wines is usually lower than what might be expected, given their concentration in grapes. This is in part due to the interactions between cell walls from grapes and yeast with phenolics during red winemaking. Most of these aggregates are insoluble and end up precipitating, forming part of the lees. The objective of this study is to determine the capacity of ultrasounds and/or enzymes treatments (β-glucanase and a pectolytic enzyme) to release the anthocyanins and tannins adsorbed in the lees. The ultrasound (US) applied for 120 min slightly favored the extraction of anthocyanins and doubled tannin extraction. Shorter sonication times did not show any positive effect. The combination of β-glucanase and pectolytic enzyme was always more effective in the liberation of anthocyanins (both no-acylated and acylated anthocyanins) and tannins than the enzymes acting separately. The combination of US (120 min), β-glucanase and pectolytic enzyme showed an additive effect, increasing the extraction of phenolic compounds with respect to the individual treatments and also releasing a large quantity of low molecular weight polysaccharides, compounds of enological importance. These results of this study could be of enological interest, facilitating and accelerating the aging on lees process, through the liberation of polysaccharides and the recovery of the phenolic compounds lost during vinification.

Soluble Fibrin Complexes and Fibrinogen Heterogeneity in Diabetes mellitus

1980 ◽  
Vol 44 (03) ◽  
pp. 130-134 ◽  
Author(s):  
E B Tsianos ◽  
N E Stathakis
Keyword(s):  
Diabetes Mellitus ◽  
Molecular Weight ◽  
Plasma Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Lower Molecular Weight ◽  
Polyacrylamide Gels ◽  
Soluble Fibrin ◽  
Α2 Macroglobulin ◽  
Patients With Diabetes

SummaryThe presence of soluble fibrin complexes (SFC) measured by gel filtration of plasma on 4% agarose columns, fibrinogen heterogeneity on 3.5% SDS-polyacrylamide gels and the concentrations of several plasma proteins were evaluated in 39 patients with diabetes mellitus (DM) and 19 matched control subjects. A small but significant increase of SFC was found in DM (p<0.01). On individual basis 51.2% of the patients had increased SFC (>M + 2 SD of the controls). Polyacrylamide gel electrophoresis of the SFC showed no evidence of cross-linking or proteolysis. Plasma clots formed in the presence of EDTA and trasylol were analysed in SDS-polyacrylamide gels in a normal and two lower molecular weight fibrin bands (band I, II, III). The percentage of band I fibrinogen was in diabetics (65.3 ± 4.7%) lower than that of the controls (71.8 ± 4.5%) (p < 0.01). Fibrinogen levels, antithrombin III, α1-antitrypsin, α2-macroglobulin and plasminogen were significantly increased in DM. We suggest that in DM there is an enhancement of intravascular fibrin formation and accelerated fibrinogen degradation to lower molecular weight forms.

Permeability Enhancing and Chemotactic Activities of Lower Molecular Weight Degradation Products of Human Fibrinogen

1981 ◽  
Vol 45 (01) ◽  
pp. 090-094 ◽  
Author(s):  
Katsuo Sueishi ◽  
Shigeru Nanno ◽  
Kenzo Tanaka
Keyword(s):  
Molecular Weight ◽  
Degradation Products ◽  
Electron Microscopic Observation ◽  
Chemotactic Activity ◽  
Lower Molecular Weight ◽  
Electron Microscopic ◽  
Human Fibrinogen ◽  
Rabbit Skin ◽  
Molecular Weights ◽  
Active Fragments

SummaryFibrinogen degradation products were investigated for leukocyte chemotactic activity and for enhancement of vascular permeability. Both activities increased progressively with plasmin digestion of fibrinogen. Active fragments were partially purified from 24 hr-plasmin digests. Molecular weights of the permeability increasing and chemotactic activity fractions were 25,000-15,000 and 25,000 respectively. Both fractions had much higher activities than the fragment X, Y, D or E. Electron microscopic observation of the small blood vessels in rabbit skin correlated increased permeability with the formation of characteristic gaps between adjoining endothelial cells and their contraction.These findings suggest that lower molecular weight degradation products of fibrinogen may be influential in contributing to granulocytic infiltration and enhanced permeability in lesions characterized by deposits of fibrin and/or fibrinogen.

Composition of low molecular weight impurities in dimethylmethylpheylsiloxane rubbers with terminal hydroxyl groups

2020 ◽  
Vol 86 (11) ◽  
pp. 20-27
Author(s):  
A. M. Filippov ◽  
N. Yu. Semenkova ◽  
S. M. Gorelov ◽  
T. I. Shulyatieva ◽  
P. A. Storozhenko
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Hydroxyl Groups

Metathesis Polymerization of Phenylacetylene by Tungsten Aryloxo Complexes

1995 ◽  
Vol 60 (3) ◽  
pp. 489-497 ◽  
Author(s):  
Hynek Balcar ◽  
Jan Sedláček ◽  
Marta Pacovská ◽  
Vratislav Blechta
Keyword(s):  
Molecular Weight ◽  
Catalytic Activity ◽  
Induction Period ◽  
Average Molecular Weight ◽  
Molar Fraction ◽  
Molar Ratio ◽  
Weight Average Molecular Weight ◽  
Polymer Yield ◽  
Positive Effect ◽  
Ligand Addition

Catalytic activity of the tungsten aryloxo complexes WCl5(OAr) and WOCl3(OAr), where Ar = 4-t-C4H9C6H4, 2,6-(t-C4H9)2C6H3, 2,6-Cl2C6H3, 2,4,6-Cl3C6H2, and 2,4,6-Br3C6H2 in polymerization of phenylacetylene (20 °C, monomer to catalyst molar ratio = 1 000) was studied. The activity of WCl5(OAr) as unicomponent catalysts increases with increasing electron withdrawing character of the -OAr ligand. Addition of two equivalents of organotin cocatalysts (Me4Sn, Bu4Sn, Ph4Sn, Bu3SnH) to WCl5(O-C6H2Cl3-2,4 ,6) has only slight positive effect (slightly higher polymer yield and/or molecular weight of poly(phenylacetylene)s was achieved). However, in the case of WOCl3(O-C6H3Cl2-2, 6) catalyst, it enhances the activity considerably by eliminating the induction period. Poly(phenylacetylene)s prepared with the catalysts studied have weight-average molecular weight ranging from 100 000 to 200 000. They are trans-prevailing and have relatively low molar fraction of monomer units comprised in cyclohexadiene sequences (about 6%).

Granulocyte-macrophage colony-stimulating factor preferentially activates the 94-kD STAT5A and an 80-kD STAT5A isoform in human peripheral blood monocytes

Blood ◽  
1996 ◽  
Vol 88 (4) ◽  
pp. 1206-1214 ◽  
Author(s):  
RL Rosen ◽  
KD Winestock ◽  
G Chen ◽  
X Liu ◽  
L Hennighausen ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Human Peripheral Blood ◽  
Janus Kinase ◽  
Lower Molecular Weight ◽  
Colony Stimulating Factor ◽  
Enhancer Element ◽  
Gm Csf ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

Granulocyte-macrophage colony-stimulating factor (GM-CSF) induces immediate effects in monocytes by activation of the Janus kinase (JAK2) and STAT transcription factor (STAT5) pathway. Recent studies have identified homologues of STAT5, STAT5A, and STAT5B, as well as lower molecular weight variants of STAT5. To define the activation of the STAT5 homologues and lower molecular weight variant in human monocytes and monocytes differentiated into macrophages by culture in macrophage- CSF (M-CSF), we measured the GM-CSF induced tyrosine phosphorylation of STAT5A, STAT5B, and any lower molecular weight STAT5 isoforms. Freshly isolated monocytes expressed 94-kD STAT5A, 92-kD STAT5B, and an 80-kD STAT5A molecule. Whereas 94-kD STAT5A was clearly tyrosine phosphorylated and bound to the enhancer element, the gamma response region (GRR), of the Fc gamma RI gene, substantially less tyrosine phosphorylated STAT5B bound to the immobilized GRR element. Macrophages lost their ability to express the 80-kD STAT5A protein, but retained their ability to activate STAT5A. STAT5A-STAT5A homodimers and STAT5A- STAT5B heterodimers formed in response to GM-CSF. Therefore, activation of STAT5A predominates compared to STAT5B when assayed by direct immunoprecipitation and by evaluation of bound STATs to immobilized GRR. Selective activation of STAT5 homologues in addition to generation of lower molecular isoforms may provide specificity and control to genes expressed in response to cytokines such as GM-CSF.

scholarly journals The nutritional use of millet grain for food and feed: a review

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Z. M. Hassan ◽  
N. A. Sebola ◽  
M. Mabelebele
Keyword(s):  
Phenolic Compounds ◽  
Human Health ◽  
Pearl Millet ◽  
Egg Production ◽  
Positive Impact ◽  
The Body ◽  
Broiler Performance ◽  
Food And Feed ◽  
High Antioxidant Activity ◽  
Positive Effect

AbstractWorldwide, millets are regarded as a significant grain, however, they are the least exploited. Millet grain is abundant in nutrients and health-beneficial phenolic compounds, making it suitable as food and feed. The diverse content of nutrients and phenolic compounds present in finger and pearl millet are good indicators that the variety of millet available is important when selecting it for use as food or feed. The phenolic properties found in millets compromise phenolic acids, flavonoids, and tannins, which are beneficial to human health. Moreover, finger millet has an exceptionally unique, more abundant, and diverse phenolic profile compared to pearl millet. Research has shown that millet phenolic properties have high antioxidant activity. The presence of phytochemicals in millet grains has positive effect on human health by lowering the cholesterol and phytates in the body. The frantic demands on maize and its uses in multiple industries have merited the search for alternative grains, to ease the pressure. Substitution of maize with pearl and finger millets in the diets of different animals resulted in positive impact on the performance. Including these grains in the diet may improve health and decrease the risks of diseases. Pearl millet of 50% or more can be used in broiler diets without adversely affecting broiler performance or egg production. Of late, millet grain has been incorporated in other foods and used to make traditional beverages. Thus, the core aim of this review is to provide insight and comprehension about the nutritional and phenolic status of millets and their impact on human and livestock.

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