scholarly journals Development of a Novel Enzymatic Pretreatment for Improving the Digestibility of Protein in Feather Meal

2019 ◽  
Vol 1 (4) ◽  
pp. 475-484 ◽  
Author(s):  
Pfeuti ◽  
Osborne ◽  
Shoveller ◽  
Ignatz ◽  
Bureau

This study describes the process of developing an enzymatic pretreatment to improvethe nutritional value of feather meal (FeM). In a first experiment, a full factorial design was used toexamine the effects of various incubation conditions on the solubilization of nitrogen in FeM. Weincubated FeM for 3 h with various levels of a commercial alkaline serine protease (Savinase®16L), sodium sulphite (Na2SO3), and digestion buffer. A Savinase® 16L level of 3% (%FeM v/w),Na2SO3 level of 3% (%FeM w/w), and digestion buffer level of 500% (%FeM w/w) were identifiedas the optimal conditions. Under these optimal conditions, 45% of the nitrogen in FeM wassolubilized. In a second experiment, we evaluated the effect of more economically sustainableincubation conditions on the in vitro digestibility of protein (pepsin-HCl digestibility andmultistep protein evaluation) in FeM. Two FeMs were incubated with 0.5% Savinase® 16L (%FeMv/w), 2% Na2SO3 (%FeM w/w), and 200% buffer (%FeM w/w) for 24 h. The pretreatment improvedpepsin-HCl digestibility by 7%–16% and the total tract degradable protein content by 14%–50%.Accordingly, this novel pretreatment could be applied in the animal feed industry to improve thenutritional value of FeM.

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Engkus Ainul Yakin ◽  
Sri Sukaryani ◽  
Catur Suci Purwati ◽  
Desi Lestari

The purpose of this study was to determine the effect of the addition of tofu waste in concentrate feed on the nutritional value of ruminant animal feed. Concentrate feed used in this study uses concentrated feed for sheep. Tofu waste was obtained from the place of making tofu in Girimarto sub-district, Wonogiri Regency. The research method used was concentrate feed for sheep given the addition of tofu waste. The study used 3 treatments namely T0 = Concentrate without the addition of tofu waste (control), T1 = Concentrate with 10% tofu waste addition, and T2 = Concentrate with 20% tofu waste addition. The results of the study were analyzed in the laboratory to determine the nutritional value of sheep feed. The study was designed with a completely randomized design. Observation parameters of nutritional value of feed include dry matter, crude protein, crude fiber and in vitro digestibility. The results showed that dry matter content was 78.54 ± 0.23% and crude protein was 16.63 ± 0.46% with the addition of tofu waste until 20% showed a significant difference, whereas crude fiber was 20.37 ± 0.48 % showed no significant difference, while the dry matter digestibility and organic matter digestibility  showed no significant difference. The conclusion of this study was the addition of tofu waste in the concentrate had an effect on the dry matter and crude protein, but not significantly different on crude fiber. The addition of tofu waste to the concentrate did not affect the digestibility of dry matter or the digestibility of organic matter.Key words : Animal feed, concentrate,  in vitro digestibility, tofu waste


2021 ◽  
Vol 51 (4) ◽  
pp. 437-443
Author(s):  
S.M.P. Teixeira ◽  
C.S.A.M. Maduro Dias ◽  
C.F.M. Vouzela ◽  
J.S. Madruga ◽  
A.E.S. Borba

This study evaluated the effects of sodium hydroxide (NaOH) treatment on the nutritional value of Arundo donax (A. donax). Its ultimate goal was to develop an environmentally friendly animal feed alternative that could be produced sustainably while combatting the spread of A. donax. Plants were collected and dried at 60 °C in an oven with controlled air circulation to determine the dry matter (DM) content. The dry plant material was then sprinkled with an NaOH solution of 2%, 4%, 6%, or 8% and placed in leak-proof containers for four weeks. The chemical and in vitro digestibility properties of treated and untreated A. donax samples were analysed in triplicate. The treatment led to significant decreases in neutral detergent fibre (NDF) from 81.06% DM to 69.39% DM, acid detergent fibre (ADF) from 46.99% DM to 43.19% DM, and ether extract (EE) from 2.06% DM to 1.39% DM, in the untreated samples compared with those treated with 8% NaOH, respectively. In addition, DM digestibility increased from 24.61% to 33.78%, ash content from 11.75% DM to 19.92% DM, and ADL from 7.43% M to 15.38% DM. Thus, treatment of A. donax with an 8% solution of NaOH concentration improved its nutritional value.


2017 ◽  
Vol 38 (4) ◽  
pp. 2129
Author(s):  
Samantha Mariana Monteiro Sunahara ◽  
Marcela Abbado Neres ◽  
Jaqueline Rocha Wobeto Sarto ◽  
Caroline Daiane Nath ◽  
Kácia Carine Scheidt ◽  
...  

The goal of this study was to assess the dehydration curve and nutritional value of Tifton 85 bermudagrass at two cutting heights from ground level (4 and 8 cm) during 120 days of storage in a closed shed. The dehydration curve was determined using samples from the entire plant at eight different times. The experimental design consisted of randomized blocks with plots subdivided per times and five replicates. The second step consisted of assessing the nutritional value of the stored Tifton 85 bermudagrass in randomized blocks with plots subdivided per times and two treatments per plot: cutting height of four and eight centimeters from the ground, and five different times for the subplots, with five replicates. Dehydration of Tifton 85 bermudagrass at the two heights occurred in 48 hours, considered an ideal time for hay drying. The dry matter content responded quadratically to the time of storage of the two heights, only differing during baling and after 120 days of storage. Crude protein content had a quadratic behavior in the two cutting heights, with the smallest value after 30 days of storage (107.0 g kg-1) and the largest after 90 days (147.8 g kg-1) in the cutting height of eight centimeters. The ether extract exhibited a quadratic behavior in the two cutting heights, only differing after 90 days of storage. The neutral detergent fiber content had linear positive response according to the time of storage, with no difference between the cutting heights. For the neutral detergent fiber content in the two cutting heights, the quadratic regression model was the best fit to the data, differing between the heights after 30 and 60 days of storage. In vitro dry matter digestibility and in vitro cell wall digestibility values of the stored hay were lower than the values obtained at the time of cutting. Cutting performed at four centimeters from the ground was the most suitable for hay production due to higher dry matter production and nutritional value without difference between bailing treatments. Hay storage caused undesirable changes in the nutritional value, especially in fiber content and in vitro digestibility.


2005 ◽  
Vol 48 (spe) ◽  
pp. 161-168 ◽  
Author(s):  
Lúcia de Fátima Araújo ◽  
Ariosvaldo Nunes Medeiros ◽  
Américo Perazzo Neto ◽  
Líbia de Sousa Conrado Oliveira ◽  
Flávio Luiz Honorato da Silva

The microbial protein bioconversion of cactus pear by yeast in solid medium was studied. Three cultivation variables used were: inoculum's concentrations (5, 10 and 15 %), substrate layer thickness (2, 4 and 6 cm) and temperature (30, 34 and 38 ºC). The rate of dry matter production and total protein were determined. Results obtained were variance analysis, gross energy and in vitro dry matter digestibility. The maximum protein amount achieved for the conditions studied in the present work was higher than 26 %, which was compatible or greater than those of conventional concentrates of protein supplements used for animal feed. The protein concentrate of cactus pear had a higher in vitro digestibility index (95.8 %) and did not show any changes in the gross energy value when compared to that of the cactus pear in natura.


2014 ◽  
Vol 8 (2) ◽  
pp. 92-103 ◽  
Author(s):  
Zhang Yin ◽  
Wang Xinhui ◽  
Wang Wei ◽  
Zhang Jiaming

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250351
Author(s):  
Giorgia Meineri ◽  
Alessia Candellone ◽  
Sonia Tassone ◽  
Pier Giorgio Peiretti ◽  
Erica Longato ◽  
...  

Fresh mechanically deboned meat (MDM) is usually claimed as high-quality ingredient on dry pet food recipes and this aspect may positively influence consumer choice. It is important to determine the scientifically sustainability of this claim and to assess the microbiological safety of MDM inclusion in dry pet food. Objectives were: 1) to evaluate the effect of inclusion of MDM in dry dog food on fatty acid profile and in vivo and in vitro digestibility, proposing a new system (DaisyII Incubator) to measure the in vitro digestibility for dogs; 2) to compare palatability of dry dog food containing MDM with dry dog food in which meat by-products (MBP) are the only animal protein sources; 3) to determine, whether or not, the inclusion of that ingredient changes the microbiology and the storage quality. Results indicated that MDM product was characterized by significant higher nutritional value in terms of fatty acids profile, in vitro digestibility (HV-IVD method) and lower palatability than the MBP product. Microbiological risk assessment showed no microbiological hazards for either product. After 6-months storage, the total mesophilic bacterial count ranged between 1.77 and 2.09 log CFU/g feed, while polyamine values were higher in the MDM (0.37 g/kg) than in the MBP (0.27 g/kg). The DaisyII Incubator was found to be a valid instrument for studying in vitro digestibility also for dogs, providing data simply, quickly, with less variability and costs than in vivo trials. In conclusion, MDM inclusion in dry dog food is microbiologically safe and it can improve its nutritional quality, at the expense of a reduced palatability. The higher polyamine levels fount in MDM-enriched petfood after 6-months storage, however, may represent a possible hazard, and further studies are still warranted.


2017 ◽  
Vol 2 (2) ◽  
pp. 1-6
Author(s):  
H. Husnaeni ◽  
M. Arifin Amril ◽  
Sjamsuddin Rasjid

This research aims to improve the digestibility and nutritional value of rice straw as a ruminant animal feed and determine the effect of long soaking in sea water of rice straw on digestibility in vitro dry matter and organic matter. The used materials in this study are a bales machine, weighing scales, rapiah rope, poly bags, rice straw varieties Ciliwung obtained from rice fields in Bantimurung, sea water with 2.71% saline, as well as tools and chemicals for analysis of in vitro dry matter and organic matter digestibility with pepsin, calculus method. This study is done based on Randomized Complete  Design (RCD) with 5 treatments and 4 replications. Variance based on the long soaking treatment gives the nature of the response curve linear on the digestibility of dry matter and also to organic matter digestibility. The magnitude of the correlation length relationship soaking rice straw and with sea water for in vitro dry matter and organic matter digestibility that is 96.5% and 96.8% respectively. Based on the results of research and discussion can be concluded that rice straw is soaked with sea water 3-12 days producing dry matter digestibility and organic matter are higher than the rice straw that is not soaked with sea water. Soaking of the sea water for 3-12 days increase the digestibility of dry matter and organic matter.


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