Confirmation of Glyphosate-Resistant Horseweed (Conyza canadensis) in Montana Cereal Production and Response to POST Herbicides

2017 ◽  
Vol 31 (6) ◽  
pp. 799-810 ◽  
Author(s):  
Vipan Kumar ◽  
Prashant Jha ◽  
Amit J. Jhala
Keyword(s):  
Great Plains ◽  
Dose Response ◽  
Best Management Practices ◽  
Management Practices ◽  
Dry Weight ◽  
Shoot Dry Weight ◽  
Greenhouse Study ◽  
Whole Plant ◽  
Cereal Production ◽  
Sites Of Action

In recent years, horseweed has become an increasing problem in Montana. To confirm and characterize the level of glyphosate resistance, seeds were collected from putative glyphosate-resistant (GR) horseweed (GR-MT) plants in a wheat–fallow field in McCone County, MT. Known GR (GR-NE) and glyphosate-susceptible (GS-NE) horseweed accessions from Lincoln, NE, were included for comparison in dose–response and shikimate accumulation studies. Whole-plant glyphosate dose–response experiments conducted at the early- (5- to 8-cm diameter) and late- (12- to 15-cm diameter) rosette stages of horseweed indicated that GR-MT accessions had a 2.5- to 4.0-fold level of resistance to glyphosate relative to the GS-NE accession, on the basis of shoot dry weight (GR50values). The level of resistance was 3.1- to 7.9-fold on the basis of visually assessed injury estimates (I50values). At the whole-plant level, about 2.1- to 4.5-fold higher shikimate accumulation was observed in the GS-NE accession compared with the GR-MT and GR-NE accessions over a 10-d period after glyphosate was applied at 1,260 g ae ha−1. In a separate greenhouse study, all three horseweed accessions were also screened with alternate POST herbicides registered for use in wheat–fallow rotations. The majority of the tested herbicides provided ≥90% injury at the field-use rates for all three horseweed accessions 3 wk after treatment. This is the first published report on the occurrence of GR horseweed in Montana cereal production. Increased awareness and adoption of best management practices, including the use of diversified (based on multiple sites of action) herbicide programs highlighted in this study, would aid in mitigating the further spread of GR horseweed in the cereal production fields of the U.S. Great Plains.

Glyphosate-Resistant Russian-thistle (Salsola tragus) Identified in Montana and Washington

2017 ◽  
Vol 31 (2) ◽  
pp. 238-251 ◽  
Author(s):  
Vipan Kumar ◽  
John F. Spring ◽  
Prashant Jha ◽  
Drew J. Lyon ◽  
Ian C. Burke
Keyword(s):  
Best Management Practices ◽  
Management Practices ◽  
Field Experiments ◽  
Dry Weight ◽  
Effective Control ◽  
Best Management ◽  
Shoot Dry Weight ◽  
Greenhouse Study ◽  
Whole Plant ◽  
Site Field

Two putative glyphosate-resistant (GR) Russian-thistle accessions were collected from fallow fields (wheat-fallow rotation): one from Choteau County, MT (MT-R), and a second from Columbia County, WA (WA-R) in summer/fall of 2015. Greenhouse and outdoor/field whole-plant dose-response studies were conducted to confirm and characterize the levels of glyphosate resistance in these GR accessions relative to known glyphosate-susceptible accessions (MT-S and WA-S from MT and WA, respectively). Based on GR50values of the progeny plants, the MT-R accession exhibited 4.5-fold and 5.9-fold resistance to glyphosate relative to the MT-S accession under greenhouse and outdoor conditions, respectively. The WA-R accession showed 3.0- to 5.0-fold resistance relative to the WA-S accession in greenhouse experiments, and 1.9- to 7.5-fold resistance in multi-site field experiments. In a separate greenhouse study on alternative POST herbicides to control GR Russian-thistle, bicyclopyrone plus bromoxynil, bromoxynil plus fluroxypyr, bromoxynil plus pyrasulfotole, bromoxynil plus MCPA, paraquat alone, paraquat plus metribuzin, saflufenacil alone, saflufenacil plus 2,4-D, and 2,4-D plus bromoxynil plus fluroxypyr provided effective control (≥95%) and shoot dry weight reduction (up to 98%) of GR accessions. This research confirms the first global case of field-evolved GR Russian-thistle. Best management practices (BMPs); including alternative, effective herbicide programs (based on multiple mechanisms of action highlighted in this study) need immediate implementation to prevent further spread of GR or evolution of multiple HR Russian-thistle populations in this region.

scholarly journals Effect of Soil Amendments on Growth of Pentas in a Simulated Landscape

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 790B-790
Author(s):  
Sudeep Vyapari* ◽  
S.M. Scheiber ◽  
Richard C. Beeson
Keyword(s):  
Best Management Practices ◽  
Soil Type ◽  
Management Practices ◽  
Soil Amendments ◽  
Dry Weight ◽  
Initial Growth ◽  
Growth Indices ◽  
Root Dry Weight ◽  
Shoot Dry Weight ◽  
Top Soil

During Fall 2003, a study was conducted to determine the effect of soil amendments on growth and response of Pentas lanceolata `New Look Red' in the landscape. Pentas were grown in 250L drainage lysimeters in an open-sided clear polyethylene covered shelter filled with local top soil (Apopka fine sand). The treatments used were non-amended top soil (control) and soil amended with either compost (5% by volume) or clay (5% by volume) in the top 15 cm. Best Management Practices were followed. Irrigation frequency and rate were regulated using a tensiometer-controlled automatic irrigation system. When plant available water in each soil type had declined to 70% or less, the plants were irrigated back to field capacity. Data were recorded on initial and final growth indices, shoot dry weight, and root dry weight. Final growth indices between control and soil amended with compost were not different; however, growth in the clay-based soil was significantly less than the compost-based soil type. The mean shoot dry weight (77.2 g) produced from plants in compost amended soil type was significantly higher than either control (57.45 g) or clay amended (54.92 g) soil types. No significant differences were found for either initial growth indices or root dry weight among the three treatments.

scholarly journals (111) Establishment of Root-bound Plumbago auriculata Lam. in a Landscape

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1078D-1078
Author(s):  
Sudeep Vyapari ◽  
Edmund L. Thralls ◽  
Michele S. Scheiber
Keyword(s):  
Best Management Practices ◽  
Management Practices ◽  
Dry Weight ◽  
Growth Indices ◽  
Root Dry Weight ◽  
Shoot Dry Weight ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
Randomized Design ◽  
Harvest Dates

A study was conducted to evaluate establishment of root-bound vs. nonroot-bound container-grown Plumbago auriculata Lam. in a landscape. A total of 144 plants were transplanted from #1 containers in a rain-out shelter at Mid-Florida Research and Education Center, Apopka, Fla., in June 2004. The field soil type was amended with composted yard waste. The three treatment types used for the study were: 1) root-bound plants; 2) root-bound plants with a vertical slice made through the root ball at a 90° angle; and 3) nonroot-bound plants. To evaluate the effect of these three treatments during the course of establishment period, harvesting was done once every 2 weeks. Data on growth indices (height × width × width), shoot dry weight, root dry weight, and length of the longest root were recorded. The experimental design was a completely randomized design consisting of three treatments, 12 harvest dates (days after planting), and four replicates per harvest date. Plants were maintained according to the best management practices recommended by the UF/IFAS, and were irrigated once a day using microirrigation. Experimental data were analyzed for significance of correlation among variables using SAS version 9.1. Results of the correlation and regression analysis indicated that the increase in the shoot dry weights (g), root dry weights (g), growth indices (m3), and root: shoot ratio had significant relationship with the harvest dates. Correlation among harvest dates and shoot dry weight, root dry weight, or growth indices was found to be positive. However, results of the study indicated that as the number of days after planting increased, the root to shoot ratio decreased.

First Report of Kochia (Bassia scoparia) with Cross-Resistance to Dicamba and Fluroxypyr in Western Kansas

2019 ◽  
Vol 33 (2) ◽  
pp. 335-341 ◽  
Author(s):  
Vipan Kumar ◽  
Randall S. Currie ◽  
Prashant Jha ◽  
Phillip W. Stahlman
Keyword(s):  
Great Plains ◽  
Dose Response ◽  
Cover Crops ◽  
Gene Copy Number ◽  
Dry Weight ◽  
Gene Copy ◽  
Cross Resistance ◽  
Control Programs ◽  
Gene Copies ◽  
Whole Plant

AbstractEvolution and rapid spread of herbicide-resistant (HR) kochia has become a significant challenge for growers in the U.S. Great Plains. The main objectives of this research were to confirm and characterize the response of putative auxinic HR (Aux-HR) kochia accessions (designated as KS-4A, KS-4D, KS-4H, KS-10A, KS-10-G, and KS-10H) collected from two different corn fields near Garden City, KS, to dicamba and fluroxypyr and to determine the EPSPS gene copy number to detect whether those accessions were also resistant to glyphosate. Single-dose experiments indicated that putative Aux-HR kochia accessions had 78% to 100% and 85% to 100% survivors when treated with dicamba (560 g ae ha−1) and fluroxypyr (235 g ae ha−1), respectively. Whole-plant dicamba dose–response studies revealed that the selected Aux-HR accessions had 2.9- to 15.1- and 3.1- to 9.4-fold resistance to dicamba relative to two susceptible accessions (MT-SUS and KS-SUS). In a separate fluroxypyr dose–response experiment, the selected Aux-HR accessions also exhibited 3.8- to 7.3- and 3.0- to 8.6-fold resistance to fluroxypyr on the basis of shoot fresh and dry weight responses, respectively. The confirmed Aux-HR kochia accessions also had 3 to 13 EPSPS gene copies relative to MT-SUS and KS-SUS accessions (each with 1 EPSPS gene copy). These results suggest that the putative Aux-HR kochia accessions from Kansas had developed moderate to high levels of cross-resistance to dicamba and fluroxypyr and low to high levels of resistance to glyphosate. This is the first confirmation of kochia accessions with cross-resistance to dicamba and fluroxypyr in Kansas. Growers should use diverse kochia control programs, including the proper use of dicamba and fluroxypyr stewardship, use of cover crops, occasional tillage, diversified crop rotations, and alternative effective herbicides to prevent further evolution and spread of Aux-HR kochia on their fields.

scholarly journals Whole-Plant and Seed Bioassays for Resistance Confirmation

Weed Science ◽  
2015 ◽  
Vol 63 (SP1) ◽  
pp. 152-165 ◽  
Author(s):  
Nilda R. Burgos
Keyword(s):  
Best Management Practices ◽  
Crop Production ◽  
Management Practices ◽  
List Type ◽  
Plant Parts ◽  
Resistance Evolution ◽  
Whole Plant ◽  
Weedy Species ◽  
Whole Plants ◽  
Sites Of Action

Much of agriculture-related research today involves weed resistance to herbicides. Resistance evolution is perhaps the strongest driver for the quest for new herbicide targets, novel weed intervention technologies, and the promotion of best management practices for sustainable crop production (Burgos et al., 2006; Norsworthy et al. 2012; Vencill et al. 2012). To date, 222 weedy species collectively have evolved resistance to 150 herbicides representing 21 sites of action (Heap 2014). For decades, scientists have developed numerous protocols for resistance confirmation using seeds, different plant parts, or whole plants. These have been reviewed by Beckie et al. (2000) and Burgos et al. (2013). We draw from these and other sources to present general guidelines for resistance confirmation that students and new researchers can use in planning their experiments. The most immediate questions that stakeholders seek to answer with resistance bioassays include:1.Is the population resistant?2.What is the level of resistance?3.What alternative herbicides can be used?

scholarly journals Variable response of kochia [Kochia scoparia (L.) Schrad.] to auxinic herbicides dicamba and fluroxypyr in Montana

2015 ◽  
Vol 95 (5) ◽  
pp. 965-972 ◽  
Author(s):  
Prashant Jha ◽  
Vipan Kumar ◽  
Charlemagne A. Lim
Keyword(s):  
Great Plains ◽  
Dose Response ◽  
Weed Management ◽  
Dry Weight ◽  
Northern Great Plains ◽  
Variable Response ◽  
Kochia Scoparia ◽  
Management Tools ◽  
Herbicide Resistant ◽  
Shoot Dry Weight

Jha, P., Kumar, V. and Lim, C. A. 2015. Variable response of kochia [Kochia scoparia (L.) Schrad.] to auxinic herbicides dicamba and fluroxypyr in Montana. Can. J. Plant Sci. 95: 965–972. Herbicide-resistant kochia is an increasing concern for growers in the Northern Great Plains of United States and Canada. The objective of the research was to characterize the response of the three putative auxinic herbicide-resistant kochia inbreds (derived from accessions collected from wheat/chemical fallow fields in northern Montana) to dicamba and fluroxypyr relative to a susceptible (SUS) inbred. A dicamba dose-response study indicated that the three putative resistant inbreds (Chot-01, Chot-02, and Chot-03) had R/S ratios of 1.3 to 6.1 based on the visible control response (I50 values), and R/S ratios of 1.5 to 6.8 based on the shoot dry weight response (GR50 values). Dose-response experiments with fluroxypyr determined I50 R/S ratios of 1.4 to 5.7 and GR50 R/S ratios of 1.6 to 4.0 for the three putative resistant inbreds. The selected inbreds showed variable symptomology (phenotype) in response to dicamba and fluroxypyr. Among the three inbreds, Chot-01 exhibited the least epinasty, stem curling/swelling, and chlorosis/necrosis symptoms, and was resistant to dicamba and fluroxypyr. Growers should diversify their weed management tools to manage further spread of auxinic or multiple herbicide-resistant kochia in the region.

scholarly journals Whole-plant Response of Six Poinsettia Cultivars to Three Fertilizer and Two Irrigation Regimes

1996 ◽  
Vol 121 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Ursula Schuch ◽  
Richard A. Redak ◽  
James Bethke
Keyword(s):  
Leaf Area ◽  
Chlorophyll Content ◽  
Dry Weight ◽  
Shoot Biomass ◽  
Vegetative Development ◽  
Chlorophyll Contents ◽  
Shoot Dry Weight ◽  
Irrigation Regimes ◽  
Whole Plant ◽  
Leaf N

Six cultivars of poinsettia (Euphorbia pulcherrima Wind.), `Angelika White', `Celebrate 2', `Freedom Red', `Lilo Red', `Red Sails', and `Supjibi Red' were grown for 9 weeks during vegetative development under three constant-feed fertilizer treatments, 80,160, or 240 mg N/liter and two irrigation regimes, well-watered (high irrigation) or water deficient (low irrigation). Plants fertilized with 80 or 240 mg N/liter were 10% to 18% shorter, while those fertilized with 160 mg N/liter were 25 % shorter with low versus high irrigation. Leaf area and leaf dry weight increased linearly in response to increasing fertilizer concentrations. Low irrigation reduced leaf area, leaf, stem, and shoot dry weight 3670 to 41%. Cultivars responded similarly to irrigation and fertilizer treatments in all components of shoot biomass production and no interactions between the main effects and cultivars occurred. Stomatal conductance and transpiration decreased with increasing fertilizer rates or sometimes with low irrigation. Highest chlorophyll contents occurred in leaves of `Lilo Red' and `Freedom Red'. Leaves of plants fertilized with 80 mg N/liter were deficient in leaf N and had 40 % to 49 % lower leaf chlorophyll content compared to plants fertilized with 160 or 240 mg N/liter. Irrigation had no effect on leaf N or chlorophyll content. At the end of the experiment leaves of `Supjibi Red' and `Angelika White' contained higher concentrations of soluble proteins than the other four cultivars.

scholarly journals Rewarding Best Pest Management Practices via Reduced Crop Insurance Premiums

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Hugh J. Beckie ◽  
Stuart J. Smyth ◽  
Micheal D. K. Owen ◽  
Savannah Gleim
Keyword(s):  
Pest Management ◽  
Best Management Practices ◽  
Financial Incentives ◽  
Management Practices ◽  
Crop Insurance ◽  
The United States ◽  
Best Management ◽  
Policy Objectives ◽  
Sites Of Action ◽  
Industry Sectors

Despite decades of research, development, and extension on the mitigation and management of pesticide resistance, the global agricultural situation is becoming increasingly dire. Pest populations with evolved resistance to multiple pesticide sites of action are becoming the norm, with fewer remaining effective xenobiotics for control. We argue that financial incentives and not regulations are needed to encourage farmers or land managers to use best management practices recommended by academia. Although some incentives are offered by pesticide manufacturers or distributors, there is a paucity of incentives by other industry sectors and all levels of government (federal or state/provincial). Crop insurance can be important to facilitate and reward best pest management practices and address other important agricultural policy objectives. Herein, we describe possible changes to crop insurance programs in the United States and Canada through premium rate changes to incentivise clients to adopt best management practices.

Glyphosate- and Acetolactate Synthase Inhibitor–Resistant Kochia (Kochia scoparia) in Western Canada

Weed Science ◽  
2013 ◽  
Vol 61 (2) ◽  
pp. 310-318 ◽  
Author(s):  
Hugh J. Beckie ◽  
Robert E. Blackshaw ◽  
Ryan Low ◽  
Linda M. Hall ◽  
Connie A. Sauder ◽  
...  
Keyword(s):  
Dose Response ◽  
Best Management Practices ◽  
Management Practices ◽  
Acetolactate Synthase ◽  
Glyphosate Resistance ◽  
Western Canada ◽  
Wild Oat ◽  
Green Foxtail ◽  
Inhibitor Resistance ◽  
Als Inhibitor

In summer, 2011, we investigated suspected glyphosate-resistant (GR) kochia in three chem-fallow fields (designated F1, F2, F3, each farmed by a different grower) in southern Alberta. This study characterizes glyphosate resistance in those populations, based on data from dose–response experiments. In a greenhouse experiment, the three populations exhibited a resistance factor ranging from 4 to 6 based on shoot biomass response (GR50ratios), or 5 to 7 based on survival response (LD50ratios). Similar results were found in a field dose–response experiment at Lethbridge, AB, in spring 2012 using the F2 kochia population. In fall 2011, we surveyed 46 fields within a 20-km radius of the three chem-fallow fields for GR kochia. In the greenhouse, populations were screened with glyphosate at 900 g ae ha−1. Seven populations were confirmed as GR, the farthest site located about 13 km from the three originally confirmed populations. An additional GR population more than 100 km away was later confirmed. Populations were screened for acetolactate synthase (ALS)–inhibitor (thifensulfuron : tribenuron) and dicamba resistance in the greenhouse, with molecular characterization of ALS-inhibitor resistance in the F1, F2, and F3 populations. All GR populations were resistant to the ALS-inhibiting herbicide, but susceptible to dicamba. ALS-inhibitor resistance in kochia was conferred by Pro197, Asp376, or Trp574amino acid substitutions. Based upon a simple empirical model with a parameter for selection pressure, calculated from weed relative abundance and glyphosate efficacy, and a parameter for seedbank longevity, kochia, wild oat, and green foxtail were the top three weeds, respectively, predicted at risk of selection for glyphosate resistance in the semiarid Grassland region of the Canadian prairies; wild oat, green foxtail, and cleavers species were predicted at greatest risk in the subhumid Parkland region. This study confirms the first occurrence of a GR weed in western Canada. Future research on GR kochia will include monitoring, biology and ecology, fitness, mechanism of resistance, and best management practices.

scholarly journals ASSESSING THE NITROGEN FIXATION ABILITIES OF NEWLY DEVELOPED PHASEOLUS VULGARIS LINES

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 662e-662
Author(s):  
Dyremple B. Marsh ◽  
Wayne McLaughlin ◽  
James S. Beaver
Keyword(s):  
Phaseolus Vulgaris ◽  
Dry Weight ◽  
Tropical Regions ◽  
Breeding Lines ◽  
Shoot Dry Weight ◽  
Greenhouse Study ◽  
Kidney Beans ◽  
Randomized Design ◽  
Red Kidney Bean ◽  
Completely Randomized Design

Methods to improve the grain yield of red kidney bean without the addition of commercially fixed nitrogen will have significant benefits to farmers in Jamaica and other tropical regions. Red kidney beans provide a major portion of the dietary protein for most families in these regions. Our experimental objective was to evaluate the nitrogen fixing capabilities of several breeding lines of Phaseolus vulgaris when inoculated with Rhizobium strains isolated from Jamaican soils. Surface sterilized seeds of 11 Phaseolus lines were inoculated with inoculum prepared from 5 day old Rhizobium YEM mixture. Rhizobium used were T2 and B17 from Jamaica and UMR 1889. The greenhouse study was arranged as a completely randomized design. Bean lines 9056-101, 9056-98B, 8954-5 and 8954-4 showed improved nodulation and N2 fixation when inoculated with UMR 1899. The combination of breeding line 8954-5 and Rhizobium strain B17 produced the highest nodule number and shoot dry weight of 193 and 0.72 g, respectively. The Rhizobium strain B17showed some ability to compete successfully for nodule sites against known effective strains.

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