Simultaneous measurement of the fluorescence spectrum and lifetime of Rhodamine B in solution with a fluorometer based on streak-camera technologies

1995 ◽  
Vol 67 (3) ◽  
pp. 511-518 ◽  
Author(s):  
Mitsuru. Ishikawa ◽  
Motoyuki. Watanabe ◽  
Tsuyoshi. Hayakawa ◽  
Musubu. Koishi
2021 ◽  
Vol 13 (2) ◽  
pp. 46
Author(s):  
Jakub Włodarski ◽  
Miłosz Chychłowski

This article presents the latest results of rhodamine B and fluorescein solutions emissions at different weight concentrations. The shift of emitted central peak wavelength is observed to change in weight concentrations of the active material in samples. By using only two active materials photoluminescence at desired wavelength form range 524nm – 660nm can be achieved. All samples were excited sequentially by a laser source and two blue light-emitting diodes without significant changes in the emitted spectra. Full Text: PDF ReferencesJ. R. Lakowicz, Principles of Fluorescence Spectroscopy. Springer Science & Business Media, Pages 1-6, (2013). CrossRef R. Sjöback, J. Nygren, M. Kubista, "Absorption and fluorescence properties of fluorescein", Spectrochimica Acta Part A: Molecular Spectroscopy, Volume 51, L7-L21, (1995) CrossRef M. F. Al-Kadhemy, I. F. Alsharuee, A. A. D. Al-Zuky, "Analysis of the effect of the concentration of rhodamine B in ethanol on the fluorescence spectrum using the 'Gauss Mod'function", Journal of Physical Science, 22(2), 77-86, (2011). DirectLink F. M. Zehentbauer, et al., "Fluorescence spectroscopy of Rhodamine 6G: Concentration and solvent effects", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 121, 147-151, (2014) CrossRef M. F. H. Al-Kadhemy, A. A. D. Al-Zuky, H. F. Daear, "Theoretical Model for the Effect of Temperature on the Fluorescence Spectrum of Laser Dye (Rh6G) in Acetone", International Letters of Chemistry, Physics and Astronomy, 19, (2014). CrossRef A. H.Al-Hamdani, S. M. Jasim, E. M. Abbas, D. A. Hassan, M. M. Jaber, "Spectral Properties of Hybrid of Rhodamine (6G) Dyes Doped Epoxy Resin Dissolved in Chloroform", Baghdad Science Journal, 16(3 Supplement), 764-769, (2019). http://dx.doi.org/10.21123/bsj.2019.16.3(Suppl.).0764 CrossRef M. F. Al-Kadhemy, I. F. Alsharuee, A. A. D. Al-Zuky, "Analysis of the effect of the concentration of rhodamine B in ethanol on the fluorescence spectrum using the 'Gauss Mod'function", Journal of Physical Science, 22(2), 77-86, (2011). DirectLink C. Bojarski, G. Żurkowska, J. Tyrzyk, "The Concentrational Changes of the Fluorescence Decay Time of Rhodamine B in Ethanol", Zeitschrift für Naturforschung A, vol. 37, no. 1, 74-77, (1982) CrossRef M. F. Al-Kadhemy, A. Al-Zuky, I. F. Al-Sharuee, "Theoretical Model to Study the Effect of Concentration and Impurities on the Fluorescence Spectrum of Fluorescein Solution in Ethanol using Fourier Series 3× 2", International Journal of Materials Physics, 2, 75-86, (2011). DirectLink D. V. Vezenov, B. T. Mayers, D. B. Wolfe, G. M. Whitesides, "Integrated fluorescent light source for optofluidic applications", Applied Physics Letters, volume 86, 041104, (2005) CrossRef V. P. Vladev, T. A. Eftimov, W. J. Bock, "Broad-band fluorescent all-fiber source based on microstructured optical fibers", Phot. Lett. Pol., vol. 7, no. 2, 41-43, (2015) CrossRef J. Żmojda, P. Miluski, M. Kochanowicz, J. Dorosz, A. Baranowska, M. Leśniak, D. Dorosz, "Luminescent properties of active optical fibers", Phot. Lett. Pol., vol. 11, CrossRef


2019 ◽  
Vol 85 (1) ◽  
pp. 47-57
Author(s):  
Mariana Gumenna ◽  
Nina Klimenko ◽  
Alexandr Stryutsky ◽  
Alexandr Shevchuk ◽  
Viktor Kravchenko

A method for the synthesis of amphiphilic reactive oligomeric silsesquioxanes (OSS) with fragments of Rhodamine B fluorescent dye and hydroxyl groups in organic shell (OSS-Rh) by the reaction between carboxyl groups of the dye and epoxy groups of the mixture of oligomeric silsesquioxanes (OSS-Ep) was developed. The structure of the synthesized substance was characterized by the methods of IR and 1H NMR spectroscopy. The UV-spectrum of the OSS-Rh compound in dimethylformamide (DMF) solution was characterized by absorption bands of both the colored zwitterion (562 nm and 350 nm) and the colorless lactone (318 nm) forms of Rhodamine B. The absorption band at 562 nm in the spectrum of OSS-Rh in DMF solution was more intense than the analogous band in the spectrum of the original Rhodamine B. Therefore, the attachment of Rhodamine B to the silsesquioxane core of an oligomeric silsesquioxanes mixture does not have a significant effect on the position of the absorption maxima in UV-spectrum and prevents dye’s fragments from converting to the colorless lactone form. In the fluorescence spectra of both Rhodamine B and OSS-Rh, obtained using ethyl alcohol as a solvent, a peak is observed at λmax = 570 nm (λex = 500 nm). In the fluorescence spectrum of OSS-Rh obtained in DMF, a fluorescence peak is observed at λmax = 586 nm (λex = 520 nm). Consequently, the replacement of ethanol by DMF is accompanied by a bathochromic shift of the fluorescence peak of OSS-Rh. In the fluorescence spectrum of Rhodamine B at the same conditions, the peak of fluorescence is absent because of transition of the dye to the lactone form. The compounds obtained can be used in formation of functional Langmuir-Blodgett films as well as in obtaining polymer nanocomposites by covalent bonding.


Author(s):  
Rahmiana Zein ◽  
Mutia Khuratul Aini ◽  
Hermansyah Aziz

Biosorpsi zat warna Rhodamine B menggunakan cangkang Pensi (Corbicula moltkiana) telah dikaji. Percobaan dilakukan dengan system batch guna memperoleh kondisi optimum biosorspi zat warna. Kapasitas biosorpsi zat warna pada pH 2 adalah 0.9958 mg/g, dengan konsentrasi larutan mula-mula 150 mg/L waktu kontak 105 menit, massa biosorben 0.1 g, ukuran partikel 32 µm dan temperature pengeringan biosorben pada 75oC. Model isotherm Langmuir menunjukkan bahwa proses penyerapan berlangsung secara kimia dan biosorpsi homogeny dari adsorbat (Rhodamine B) pada permukaan biosorben membentuk lapisan tunggal dengan nilai R2 0.9966. Analisis XRF menunjukkan bahwa penurunan kadar unsur logam pada cangkang Pensi membuktikan bahwa proses biosorpsi berlangsung dengan pertukaran kation. Hasil analisis spektrum FT-IR membuktikan adanya interaksi antaramolekul Rhodamin B dengan gugus fungsi pada cangkang Pensi. Analisis dengan SEM memperlihatkan bahwa pori-pori cangkang Pensi telah terisi penuh oleh molekul Rhodamin B. Kondisi optimum biosorpsi telah diaplikasikan pada limbah kerupuk merah dengan kapasitas penyerapan sebesar 0,2835 mg/g.   The biosorption of Rhodamine B dyes by Pensi (Corbicula moltkiana) shell has been investigated. The experiment was conducted in batch sistem in order to obtain the optimum conditions of dye biosorption. Biosorption capacity of dye was 0.9958 mg/g at pH 2, initial concentration 150 mg/L, contact time 105 minutes, biosorbent mass 0.1 gram, particle size 32 µ m and biosorbent drying temperature was at 75oC. The Langmuir Isotherm model showed chemisorption and homogeneous biosorption process of adsorbates onto the biosorbent surface formed monolayer dye molecules on the biosorbent surface with R2 value was 0.9966. XRF analysis showed that reduction of metals unsure quantity of pensi shell indicated biosorption process was occupied through cationic exchange. The result of FTIR spectra analysis indicated an interaction between Rhodamin B molecules and functional group of pensi shell. SEM analysis showed that the pensi shell pores were completely filled by Rhodamine B molecules. The optimum condition of biosorption has been aplicated in red chips wastewater industry with biosorption capacity was 0.2835 mg/g.


2014 ◽  
Vol 134 (3) ◽  
pp. 41-46
Author(s):  
Hironori Kumazaki ◽  
Munehiro Hiramatsu ◽  
Hisakazu Oguri ◽  
Seiki Inaba ◽  
Kazuhiro Hane

2019 ◽  
Author(s):  
Candace E. Benjamin ◽  
Zhuo Chen ◽  
Olivia Brohlin ◽  
Hamilton Lee ◽  
Stefanie Boyd ◽  
...  

<div><div><div><p>The emergence of viral nanotechnology over the preceding two decades has created a number of intellectually captivating possible translational applications; however, the in vitro fate of the viral nanoparticles in cells remains an open question. Herein, we investigate the stability and lifetime of virus-like particle (VLP) Qβ - a representative and popular VLP for several applications - following cellular uptake. By exploiting the available functional handles on the viral surface, we have orthogonally installed the known FRET pair, FITC and Rhodamine B, to gain insight of the particle’s behavior in vitro. Based on these data, we believe VLPs undergo aggregation in addition to the anticipated proteolysis within a few hours of cellular uptake.</p></div></div></div>


2018 ◽  
Vol 7 (2) ◽  
pp. 131-136
Author(s):  
Nasir Ahmad

Background: On May 4th, 2016, at 12:30 district surveillance officer of Magelang Health Department received reports from Public Health Center of Bandongan about 21 students of SDN 1 Trasan who suffered from the same food-poisoning symptoms. Objective: Investigation was carried out to identify the source, how it spread and how to control it. Methods: This study used descriptive analytic and mapping the cases distribution location. The case was people experiencing symptoms of dizziness or abdominal pain or nausea or vomiting. Data analysis was done by using bivariate analysis. Data collection were done through interviews, observations and laboratory tests on the food samples. Results: The case was 50 students (from 1-6 grade students). The perceived symptoms were dizziness (77%), nausea (42%), abdominal pain (40%) and vomiting (8%). Attack rate found ranged from 14.3% to 60% with the highest Attack rate found on class three (60%). The incubation period of 15-240 minutes (mean 72.3 minutes). Calamari like positive Bacillus cereus and Rhodamine-B 10 mg/kg. Conclusion: The outbreak of food poisoning because calamari like contaminated Bacillus cereus. We suggested the school committee to provide the socialization of harmful food for the students. The teachers should restrict the permission for the food vendor to sell at school.   Keywords: Bacillus cereus, , Food Poisoning, Outbreak, Rhodamine B, School Food


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