scholarly journals Barcoded Microchips for Biomolecular Assays

2014 ◽  
Vol 87 (2) ◽  
pp. 900-906 ◽  
Author(s):  
Yi Zhang ◽  
Jiashu Sun ◽  
Yu Zou ◽  
Wenwen Chen ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Biomolecular Assays

A digital microfluidic platform for the automation of quantitative biomolecular assays

Lab on a Chip ◽  
2010 ◽  
Vol 10 (6) ◽  
pp. 685-691 ◽  
Author(s):  
Erik C. Jensen ◽  
Bharath P. Bhat ◽  
Richard A. Mathies
Keyword(s):  
Microfluidic Platform ◽  
Biomolecular Assays ◽  
Digital Microfluidic

Promoter engineering improves transcription efficiency in biomolecular assays

2021 ◽  
Vol 57 (13) ◽  
pp. 1619-1622
Author(s):  
Jisu Woo ◽  
Jung Ho Kim ◽  
Seokjoon Kim ◽  
Ki Soo Park
Keyword(s):  
High Sensitivity ◽  
Rnase H ◽  
Activity Assay ◽  
T7 Promoter ◽  
Promoter Engineering ◽  
Transcription Efficiency ◽  
Sensitivity And Selectivity ◽  
Biomolecular Assays ◽  
Diagnostic Applications

A novel T7 promoter with improved transcription efficiency has been developed. It is more suitable for diagnostic applications due to its small size and is successfully used for an RNase H activity assay with high sensitivity and selectivity.

scholarly journals Resistance Patterns, mcr-4 and OXA-48 Genes, and Virulence Factors of Escherichia coli from Apennine Chamois Living in Sympatry with Domestic Species, Italy

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 129
Author(s):  
Camilla Smoglica ◽  
Alberto Vergara ◽  
Simone Angelucci ◽  
Anna Rita Festino ◽  
Antonio Antonucci ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Red Deer ◽  
Virulence Genes ◽  
Central Italy ◽  
Resistance Mechanisms ◽  
Phenotypic Resistance ◽  
Domestic Species ◽  
Resistance Patterns ◽  
Biomolecular Assays ◽  
Potential Pathogenicity

The aim of this study was to determine and characterize potential resistance mechanisms against selected Critically Important Antibiotics in Escherichia coli isolates collected from wild and domestic ruminants living in the Maiella National Park, in Central Italy. A total of 38 isolates were obtained from red deer, Apennine chamois, cattle, sheep, and goats grazing in lands with different levels of anthropic pressure. Antimicrobial susceptibility was determined by Minimal Inhibitory Concentration testing, showing phenotypic resistance to colistin, meropenem, or ceftazidime in 9 isolates along with one bacterial strain being resistant to three of the tested antibiotics. In addition, the biomolecular assays allowed the amplification of the genes conferring the colistin (mcr-4), the carbapenems (OXA-48), penicillins and cephalosporins (TEM, SHV, CMY-1, CMY-2) resistance. In order to describe the potential pathogenicity of isolates under study, virulence genes related to Shiga toxin-producing (STEC) and enteropathogenic (EPEC) pathovars were identified. This study is the first report of mcr-4 and OXA-48 genes in resistant E. coli harboring virulence genes in Italian wildlife, with special regard to Apennine chamois and red deer species. The multidisciplinary approach used in this study can improve the early detection of emerging antibiotic resistance determinants in human-animal-environment interfaces by means of wildlife monitoring.

Applications of laser-polarized 129xe to biomolecular assays

2003 ◽  
Vol 21 (10) ◽  
pp. 1235-1239 ◽  
Author(s):  
Thomas J. Lowery ◽  
Seth M. Rubin ◽  
E.Janette Ruiz ◽  
Megan M. Spence ◽  
Nicolas Winssinger ◽  
...  
Keyword(s):  
Biomolecular Assays

scholarly journals Machine-Perception Nanosensor Platform to Detect Cancer Biomarkers

2021 ◽  
Author(s):  
Zvi Yaari ◽  
Yoona Yang ◽  
Elana Apfelbaum ◽  
Alex Settle ◽  
Quinlan Cullen ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Point Of Care ◽  
Survival Rates ◽  
Simultaneous Detection ◽  
Cancer Biomarkers ◽  
Machine Learning Algorithms ◽  
Protein Biomarkers ◽  
Recognition Elements ◽  
Biomolecular Assays ◽  
Uterine Lavage

AbstractConventional molecular recognition elements, such as antibodies, present issues for the development of biomolecular assays for use in point-of-care devices, implantable/wearables, and under-resourced settings. Additionally, antibody development and use, especially for highly multiplexed applications, can be slow and costly. We developed a perception-based platform based on an optical nanosensor array that leverages machine learning algorithms to detect multiple protein biomarkers in biofluids. We demonstrated this platform in gynecologic cancers, which are often diagnosed at advanced stages, leading to low survival rates. We investigated the platform for detection in uterine lavage samples, which are enriched with cancer biomarkers compared to blood. We found that the method enables the simultaneous detection of multiple biomarkers in patient samples, with F1-scores of ~0.95 in uterine lavage samples from cancer patients. This work demonstrates the potential of perception-based systems for the development of multiplexed sensors of disease biomarkers without the need for specific molecular recognition elements.

scholarly journals Bead injection for biomolecular assays: Affinity chromatography enhanced by bead injection spectroscopy

The Analyst ◽  
2006 ◽  
Vol 131 (7) ◽  
pp. 809 ◽  
Author(s):  
Yan Gutzman ◽  
Andrea D. Carroll ◽  
Jaromir Ruzicka
Keyword(s):  
Affinity Chromatography ◽  
Biomolecular Assays ◽  
Bead Injection

scholarly journals Selective targeting of mutually exclusive DNA G-quadruplexes: HIV-1 LTR as paradigmatic model

2020 ◽  
Vol 48 (9) ◽  
pp. 4627-4642 ◽  
Author(s):  
Martina Tassinari ◽  
Michela Zuffo ◽  
Matteo Nadai ◽  
Valentina Pirota ◽  
Adriana Carolina Sevilla Montalvo ◽  
...  
Keyword(s):  
Cellular Localization ◽  
Cooperative Interaction ◽  
Design And Synthesis ◽  
Naphthalene Diimide ◽  
Novel Approach ◽  
Wide Range ◽  
Selective Targeting ◽  
G Quadruplex ◽  
Biomolecular Assays ◽  
Hiv 1

Abstract Targeting of G-quadruplexes, non-canonical conformations that form in G-rich regions of nucleic acids, has been proposed as a novel therapeutic strategy toward several diseases, including cancer and infections. The unavailability of highly selective molecules targeting a G-quadruplex of choice has hampered relevant applications. Herein, we describe a novel approach, based on naphthalene diimide (NDI)-peptide nucleic acid (PNA) conjugates, taking advantage of the cooperative interaction of the NDI with the G-quadruplex structure and hybridization of the PNA with the flanking region upstream or downstream the targeted G-quadruplex. By biophysical and biomolecular assays, we show that the NDI-PNA conjugates are able to specifically recognize the G-quadruplex of choice within the HIV-1 LTR region, consisting of overlapping and therefore mutually exclusive G-quadruplexes. Additionally, the conjugates can induce and stabilize the least populated G-quadruplex at the expenses of the more stable ones. The general and straightforward design and synthesis, which readily apply to any G4 target of choice, together with both the red-fluorescent emission and the possibility to introduce cellular localization signals, make the novel conjugates available to selectively control G-quadruplex folding over a wide range of applications.

scholarly journals A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine,Angelica sinensis, Using a Proteomic Approach

2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Chia-Yen Hsiao ◽  
Ching-Yi Hung ◽  
Tung-Hu Tsai ◽  
Kin-Fu Chak
Keyword(s):  
Wound Healing ◽  
Herbal Medicine ◽  
Angelica Sinensis ◽  
Pharmacological Effects ◽  
Traditional Chinese Herbal Medicine ◽  
Cell Extracts ◽  
Proteomic Approach ◽  
Collagen Secretion ◽  
Biomolecular Assays ◽  
Ethanol Extracts

Angelica sinensis(AS) is a traditional Chinese herbal medicine that has been formulated clinically to treat various form of skin trauma and to help wound healing. However, the mechanism by which it works remains a mystery. In this study we have established a new platform to evaluate the pharmacological effects of total AS herbal extracts as well as its major active component, ferulic acid (FA), using proteomic and biochemical analysis. Cytotoxic and proliferation-promoting concentrations of AS ethanol extracts (AS extract) and FA were tested, and then the cell extracts were subject to 2D PAGE analysis. We found 51 differentially expressed protein spots, and these were identified by mass spectrometry. Furthermore, biomolecular assays, involving collagen secretion, migration, and ROS measurements, gave results that are consistent with the proteomic analysis. In this work, we have demonstrated a whole range of pharmacological effects associated withAngelica sinensisthat might be beneficial when developing a wound healing pharmaceutical formulation for the herbal medicine.

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