3D HPLC-MS with Reversed-Phase Separation Functionality in All Three Dimensions for Large-Scale Bottom-Up Proteomics and Peptide Retention Data Collection

2016 ◽  
Vol 88 (5) ◽  
pp. 2847-2855 ◽  
Author(s):  
Vic Spicer ◽  
Peyman Ezzati ◽  
Haley Neustaeter ◽  
Ronald C. Beavis ◽  
John A. Wilkins ◽  
...  
2017 ◽  
Vol 5 (2) ◽  
pp. 106-116
Author(s):  
Dimas Pratidina Puri Astuti ◽  
Enike Dwi Kusumawati

ABSTRAK Penelitian ini bertujuan untuk mendiskripsikan penerapan proses managemen keterampilan pada Koperasi skala besar, skala menengah dan skala kecil dan juga untuk mengetahui penerapan proses managemen keterampilan pada Koperasi skala besar, skala menengah dan skala kecil. Penelitian dilakukan pada Koperasi Susu Pujon, KUD DAU, dan KUD Karangploso dimana Koperasi tersebut tergabung dalam anggota GKSI Jawa Timur. Metode penelitian yang digunakan adalah metode  penelitian  studi kasus diskriptif yaitu  mencoba menganalisis ‘’Managerial Skill Assessment Pada Pengelola Koperasi Susu Segar Di Jawa Timur. Metode pengumpulan data dan cara mendapatkan informasi penelitan dilakukan melalui observasi partisipasi aktif dan wawancara dengan menggunakan alat bantu kuisoner. Data pada penelitian ini adalah data kualitatif , sehingga analisis data yang dihasilkan yaitu analisis deskriptif. Analis deskriptif merupakan upaya yang dilakukan dengan jalan bekerja dengan data, mengorganisasikan data, memilah-milah menjadi satuan yang dapat dikelola, mensintesiskannya, mencari dan menemukan pola, menemukan apa yang penting atau apa yang dipelajari dan memusatkan apa yang dapat diceritakan kepada orang lain dan menggunakan balance scor maupun statistika. Metode pengumpulan data dan cara mendapatkan informasi penelitan dilakukan melalui observasi partisipasi aktif dan wawancara dengan menggunakan alat bantu kuisoner, sehingga dapat diketahui pengaruh penerapan ketrampilan tehnis, ketrampilan interpersonal, ketrampilan konseptual, dan ketrampilan politik terhadap  90 responden sebagai kinerja pengelola koperasi yang berasal dari 3 koperasi. Hasil penelitian menunjukkan bahwa pengelola Koperasi telah melaksanakan managemen yang baik melalui lima pilar yaitu pilar organisasi yang membudidayakan sistem bottom up, kepemimpinan yang partisipatif, komitmen yang berorientasi pada kebutuhan customer, terlepas dari tingkat organisasi, semua manajer harus memiliki beberapa keterampilan yang penting: keterampilan teknis, keterampilan interpersonal, keterampilan konseptual, keterampilan diagnostik, dan keterampilan politik.   Abtract This study aims to design the management process of Large Scale Cooperation, Large Scale and Small Scale and also to study management process on the scale of Large Scale Cooperation, Large Scale and Small Scale. The research was conducted on Pujon Milk Cooperative, KUD DAU, and KUD Karangploso where the cooperative was incorporated in GKSI member of East Java. The research method used is descriptive case study research method, which can be analyzed. 'Managerial Skill Assessment On The Manager of Fresh Milk Cooperatives In East Java. Methods of data collection and how to obtain information research conducted through interaction and interviews using the quisioner aids. The data in this research is qualitative data, so that the data analysis is descriptive analysis. Descriptive analysts are an effort done by working with data, organizing data, sorting into manageable units, synthesizing them, finding and finding patterns, finding what is important or what is learned and focusing what can be told to others and using balance score and statistics. Methods of data collection and how to obtain research information is done through observation of active participation and interview using quisoner aids, so that can know the influence of applying of technical skill, interpersonal skill, conceptual skill, and political skill to 90 responden as performance of cooperative manager from 3 cooperative . The results showed that the manager of the Cooperative has performed good management through the five pillars of the organization that oversees the bottom up system, participative leadership, commitment focused on customer needs, technical skills, interpersonal skills, conceptual skills, skills, and political skills.


2004 ◽  
Vol 856 ◽  
Author(s):  
Michael J.A. Hore ◽  
Mohamed Laradji

ABSTRACTUsing large scale particle dynamics simulations, we investigated the effect of nanoscale rods on the dynamics of phase separation dynamics of two-component fluids in three dimensions. We found that when the nanoparticles interact more attractively with one of the two segregating component, they lead to a reduction of the rate of domain growth, and that this decrease is intensified as the nanoparticles volume fraction is increased. Furthermore, our results show that nanorods are much more effective in slowing down the kinetics than nanosphres. The dramatic effect of nanorods on the dynamics of phase separation of multi-component fluids, as opposed to nanospheres, implies that they may be used as an efficacious emulsifying agent of multi-component polymer blends.


2018 ◽  
Author(s):  
Mike Nutt ◽  
Gregory Raschke

Library spaces that blend collaboration areas, advanced technologies, and librarian expertise are creating new modes of scholarly communication. These spaces enable scholarship created within high-definition, large-scale visual collaborative environments. This emergent model of scholarly communication can be experienced within those specific contexts or through digital surrogates on the networked Web. From experiencing in three dimensions the sermons of John Donne in 1622 to interactive media interpretations of American wars, scholars are partnering with libraries to create immersive digital scholarship. Viewing the library as a research platform for these emergent forms of digital scholarship presents several opportunities and challenges. Opportunities include re-engaging faculty in the use of library space, integrating the full life-cycle of the research enterprise, and engaging broad communities in the changing nature of digitally-driven scholarship. Issues such as identifying and filtering collaborations, strategically managing staff resources, creating surrogates of immersive digital scholarship, and preserving this content for the future present an array of challenges for libraries that require coordination across organizations. From engaging and using high-technology spaces to documenting the data and digital objects created, this developing scholarly communication medium brings to bear the multifaceted skills and organizational capabilities of libraries.


Atmosphere ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 811
Author(s):  
Yaqin Hu ◽  
Yusheng Shi

The concentration of atmospheric carbon dioxide (CO2) has increased rapidly worldwide, aggravating the global greenhouse effect, and coal-fired power plants are one of the biggest contributors of greenhouse gas emissions in China. However, efficient methods that can quantify CO2 emissions from individual coal-fired power plants with high accuracy are needed. In this study, we estimated the CO2 emissions of large-scale coal-fired power plants using Orbiting Carbon Observatory-2 (OCO-2) satellite data based on remote sensing inversions and bottom-up methods. First, we mapped the distribution of coal-fired power plants, displaying the total installed capacity, and identified two appropriate targets, the Waigaoqiao and Qinbei power plants in Shanghai and Henan, respectively. Then, an improved Gaussian plume model method was applied for CO2 emission estimations, with input parameters including the geographic coordinates of point sources, wind vectors from the atmospheric reanalysis of the global climate, and OCO-2 observations. The application of the Gaussian model was improved by using wind data with higher temporal and spatial resolutions, employing the physically based unit conversion method, and interpolating OCO-2 observations into different resolutions. Consequently, CO2 emissions were estimated to be 23.06 ± 2.82 (95% CI) Mt/yr using the Gaussian model and 16.28 Mt/yr using the bottom-up method for the Waigaoqiao Power Plant, and 14.58 ± 3.37 (95% CI) and 14.08 Mt/yr for the Qinbei Power Plant, respectively. These estimates were compared with three standard databases for validation: the Carbon Monitoring for Action database, the China coal-fired Power Plant Emissions Database, and the Carbon Brief database. The comparison found that previous emission inventories spanning different time frames might have overestimated the CO2 emissions of one of two Chinese power plants on the two days that the measurements were made. Our study contributes to quantifying CO2 emissions from point sources and helps in advancing satellite-based monitoring techniques of emission sources in the future; this helps in reducing errors due to human intervention in bottom-up statistical methods.


Soft Matter ◽  
2021 ◽  
Author(s):  
Claudio Maggi ◽  
Matteo Paoluzzi ◽  
Andrea Crisanti ◽  
Emanuela Zaccarelli ◽  
Nicoletta Gnan

We perform large-scale computer simulations of an off-lattice two-dimensional model of active particles undergoing a motility-induced phase separation (MIPS) to investigate the systems critical behaviour close to the critical point...


2016 ◽  
Vol 44 (4) ◽  
pp. 522-542 ◽  
Author(s):  
Christofer Berglund

After the Rose Revolution, President Saakashvili tried to move away from the exclusionary nationalism of the past, which had poisoned relations between Georgians and their Armenian and Azerbaijani compatriots. His government instead sought to foster an inclusionary nationalism, wherein belonging was contingent upon speaking the state language and all Georgian speakers, irrespective of origin, were to be equals. This article examines this nation-building project from a top-down and bottom-up lens. I first argue that state officials took rigorous steps to signal that Georgian-speaking minorities were part of the national fabric, but failed to abolish religious and historical barriers to their inclusion. I next utilize a large-scale, matched-guise experiment (n= 792) to explore if adolescent Georgians ostracize Georgian-speaking minorities or embrace them as their peers. I find that the upcoming generation of Georgians harbor attitudes in line with Saakashvili's language-centered nationalism, and that current Georgian nationalism therefore is more inclusionary than previous research, or Georgia's tumultuous past, would lead us to believe.


Geophysics ◽  
1989 ◽  
Vol 54 (3) ◽  
pp. 350-358 ◽  
Author(s):  
G. Nolet ◽  
R. Sleeman ◽  
V. Nijhof ◽  
B. L. N. Kennett

We present a simple algorithm for computing the acoustic response of a layered structure containing three‐dimensional (3-D) irregularities, using a locked‐mode approach and the Born approximation. The effects of anelasticity are incorporated by use of Rayleigh’s principle. The method is particularly attractive at somewhat larger offsets, but computations for near‐source offsets are stable as well, due to the introduction of anelastic damping. Calculations can be done on small minicomputers. The algorithm developed in this paper can be used to calculate the response of complicated models in three dimensions. It is more efficient than any other method whenever many sources are involved. The results are useful for modeling, as well as for generating test signals for data processing with realistic, model‐induced “noise.” Also, this approach provides an alternative to 2-D finite‐difference calculations that is efficient enough for application to large‐scale inverse problems. The method is illustrated by application to a simple 3-D structure in a layered medium.


1990 ◽  
Vol 43 (2) ◽  
pp. 301-311 ◽  
Author(s):  
WINNIE Y. YOUNG ◽  
JANIS S. HOUSTON ◽  
JAMES H. HARRIS ◽  
R. GENE HOFFMAN ◽  
LAURESS L. WISE

2006 ◽  
Vol 62 (1) ◽  
pp. 165-165
Author(s):  
M. Luz Godino Salido ◽  
Paloma Arranz Mascarós ◽  
Rafaél López Garzón ◽  
M. Dolores Gutiérrez Valero ◽  
John N. Low ◽  
...  

Some of the data collection details for compound (VIII) were incorrectly given in Table 1 of Godino Salido et al. (2004). The data for compound VIII in this paper were collected using synchrotron radiation at the Daresbury SRS station 9.8, λ = 0.6935 Å (Cernik et al., 1997; Clegg, 2000). The data were collected using a Bruker SMART 1K CCD diffractometer using ω rotation with narrow frames. The computer program used in the data collection was SMART (Bruker, 2001) and for cell refinement and data reduction SAINT (Bruker, 2001).


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