Poly(tertiary amide acrylate) Copolymers Inspired by Poly(2-oxazoline)s: Their Blood Compatibility and Hydration States

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

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Calcium phosphate phase transition in the presence of Aminosilane

Acta Crystallographica Section A Foundations and Advances ◽

10.1107/s205327331409932x ◽

2014 ◽

Vol 70 (a1) ◽

pp. C67-C67

Author(s):

Babak Mostaghaci ◽

Brigitta Loretz ◽

Robert Haberkorn ◽

Guido Kickelbick ◽

Claus-Michael Lehr

Keyword(s):

Phase Transition ◽

Calcium Phosphate ◽

Blood Compatibility ◽

Transfection Efficiency ◽

Hek293 Cells ◽

Step Method ◽

Calcium Phosphate Nanoparticles ◽

Amine Groups ◽

The Impact

Calcium phosphate has been the point of interest for in vitro gene delivery for many years because of its biocompatibility and straight forward application. However, there are some limitations regarding in vivo administration of these particles mostly because of vast agglomeration of the particles and lack of strong bond between the particles and pDNA. We introduced a simple single step method to functionalize calcium phosphate nanoparticles with Aminosilanes having a different number of amine groups. The nanoparticles were characterized chemically and structurally and their toxicity and interaction with pDNA were studied as well. Results revealed that different crystalline phase of calcium phosphate nanoparticles (Brushite and Hydroxyapatite) with a size below 150 nm were prepared, depending on conditions of synthesis and phase, each with a narrow size distribution. The aminosilane agents caused oriented nucleation and growth of crystallites and can decrease the pH for producing hydroxyapatite phase. The phenomenon could be revealed with the presence of anisotropy in the structure of synthesized hydroxyapatite. The number of amine groups in the Aminosilane agent could change the phase transition pH. Brushite particles revealed to have stronger interaction with pDNA mostly because of their higher positive surface charge. Both particles showed blood compatibility and negligible toxicity. Transfection experiment revealed the capability of both brushite and hydroxyapatite particles to transfect A549 and HEK293 cells. The new modified nanoparticles can be stored in a dried state and re-dispersed easily at the time of administration. Moreover, the transfection efficiency is higher in comparison with conventional calcium phosphate. This study showed the impact of presence and type of the modifying agent on the crystal structure and the amount of surface functionalization of nanoparticles, which in consequence influenced their interaction with cells.

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Poly(Vinylamine) Derived N-Doped C-Dots with Antimicrobial and Antibiofilm Activities

C – Journal of Carbon Research ◽

10.3390/c7020040 ◽

2021 ◽

Vol 7 (2) ◽

pp. 40

Author(s):

Semiha Duygu Sutekin ◽

Mehtap Sahiner ◽

Selin Sagbas Suner ◽

Sahin Demirci ◽

Olgun Güven ◽

...

Keyword(s):

Blood Clotting ◽

Minimum Bactericidal Concentration ◽

Blood Compatibility ◽

Weight Ratio ◽

Gram Negative Bacteria ◽

Biofilm Inhibition ◽

E Coli ◽

Gram Positive Bacteria ◽

Doped Carbon Dots ◽

Nitrogen Doped Carbon

Nitrogen-doped carbon dots (N-doped C-dots) was synthesized by using poly(vinyl amine) (PVAm) as a nitrogen source and citric acid (CA) as a carbon source via the hydrothermal method. Various weight ratios of CA and PVAm (CA:PVAm) were used to synthesize N-doped C-dots. The N-doped C-dots revealed emission at 440 nm with excitation at 360 nm and were found to increase the fluorescence intensity with an increase in the amount of PVAm. The blood compatibility studies revealed no significant hemolysis for N-doped C-dots that were prepared at different ratios of CA:PVAm for up to 500 μg/mL concentration with the hemolysis ratio of 1.96% and the minimum blood clotting index of 88.9%. N-doped C-dots were found to be more effective against Gram-positive bacteria than Gram-negative bacteria, with the highest potency on Bacillus subtilis (B. subtilis). The increase in the weight ratio of PVAm in feed during C-dots preparation from 1 to 3 leads to a decrease of the minimum bactericidal concentration (MBC) value from 6.25 to 0.75 mg/mL for B. subtilis. Antibiofilm ability of N-doped C-dots prepared by 1:3 ratio of CA:PVAm was found to reduce %biofilm inhibition and eradication- by more than half, at 0.78 mg/mL for E. coli and B. subtilis generated biofilms and almost destroyed at 25 mg/mL concentrations.

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Blood Compatibility and Protein Adsorption Characteristics of Sol-Gel Derived Titania

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.192-195.889 ◽

2000 ◽

Vol 192-195 ◽

pp. 889-892 ◽

Cited By ~ 1

Author(s):

S. Takashima ◽

S. Takemoto ◽

Kanji Tsuru ◽

Satoshi Hayakawa ◽

Akiyoshi Osaka

Keyword(s):

Protein Adsorption ◽

Blood Compatibility ◽

Sol Gel ◽

Adsorption Characteristics

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Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties

Carbohydrate Polymers ◽

10.1016/j.carbpol.2020.116802 ◽

2020 ◽

Vol 248 ◽

pp. 116802 ◽

Cited By ~ 1

Author(s):

Reza Eivazzadeh-Keihan ◽

Fateme Radinekiyan ◽

Hamid Madanchi ◽

Hooman Aghamirza Moghim Aliabadi ◽

Ali Maleki

Keyword(s):

Mechanical Properties ◽

Graphene Oxide ◽

Silk Fibroin ◽

Blood Compatibility

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Blood compatibility of two different types of membrane oxygenator during cardiopulmonary bypass in infants

The International Journal of Artificial Organs ◽

10.1177/039139889401701006 ◽

1994 ◽

Vol 17 (10) ◽

pp. 543-548 ◽

Cited By ~ 10

Author(s):

Y.J. Gu ◽

P.W. Boonstra ◽

C. Akkerman ◽

H. Mungroop ◽

I. Tigchelaar ◽

...

Keyword(s):

Cardiopulmonary Bypass ◽

Blood Compatibility ◽

Hollow Fibre ◽

Membrane Oxygenator ◽

Hollow Fibre Membrane ◽

Flat Sheet ◽

Fibre Membrane ◽

Membrane Oxygenators ◽

Flat Sheet Membrane ◽

Sheet Membrane

The contact of blood with the artificial extracorporeal circuit causes a systemic inflammatory response due to blood activation. In this study, we compared two different paediatric membrane oxygenators used for extracorporeal circulation: a hollow fibre membrane oxygenator (Dideco Masterflo D-701, n=10), and a flat sheet silicone membrane oxygenator (Avecor Kolobow 800-2A, n=10). Blood compatibility was indicated by measuring complement activation as well as leukocyte and platelet activation. In patients perfused with a flat sheet membrane oxygenator, concentrations of complement split products C3a were significantly increased 30 minutes after the start of bypass (p<0.01), whereas only a mild increase of C3a was found in patients perfused with a hollow fibre membrane oxygenator. Leukocyte and platelet counts dropped uniformly in both groups after the start of bypass mainly due to hemodilution. Activation of leukocytes and platelets identified by both plasma β-glucuronidase and β-thromboglobulin was similar in both groups. Infants perfused with a flat sheet membrane oxygenator received significantly more donor blood than those perfused with a hollow fibre oxygenator (p<0.05). These results indicate that when used during paediatric cardiopulmonary bypass, a flat sheet membrane oxygenator has a higher complement activity than a hollow fibre membrane oxygenator, which is probably due to the relatively larger blood-surface contacting area of the oxygenator.

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