Transcriptome Analysis and Weighted Gene Co-expression Network Reveal Multitarget-Directed Antibacterial Mechanisms of Benzyl Isothiocyanate against Staphylococcus aureus

Aim. Antibiotic abuse contributes to the emergence of methicillin-resistant Staphylococcus aureus (MRSA). It is increasingly important to screen new antimicrobial agents for the management of MRSA infections. G. chinensis, a nontoxic Chinese herbal medicine, is considered a potential antibacterial agent. The aim of this study was to investigate the bactericidal effects of the aqueous extracts of G. chinensis on MRSA. The potential mechanisms of G. chinensis aqueous extract inhibition of the pathogenicity of MRSA in vivo are also discussed. Methods. G. chinensis aqueous extract was prepared and its antimicrobial activities were examined by determining its minimum inhibitory concentration (MIC). Biofilm biomass was determined by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). RNA sequencing (RNA-seq) was used to evaluate differentially expressed functional pathways in MRSA treated with G. chinensis aqueous extract. We validated the role of G. chinensis aqueous extract in the invasive ability and pathogenicity of MRSA in vivo using a rat infectious model. Results. The results indicated that MRSA was sensitive to the G. chinensis aqueous extracts at concentration of 31.25μg/mL. G. chinensis extract led to a reduction in dextran-dependent aggregation and biofilm formation in MRSA. Based on the transcriptome analysis, G. chinensis aqueous extracts significantly downregulated the gene expression related to biofilm formation and carbohydrate metabolism. G. chinensis aqueous extract inhibited the invasive ability and the pathogenicity of MRSA in vivo. Conclusion. The antimicrobial properties of G. chinensis aqueous extract are likely related to its modulation of MRSA biofilm formation and carbohydrate metabolism. G. chinensis aqueous extract is a promising supplementary therapy to lessen or eliminate the use of antibiotics and is a potential tool for the management of MRSA infections.

Download Full-text

Response of Staphylococcus aureus to Salicylate Challenge

Journal of Bacteriology ◽

10.1128/jb.01149-06 ◽

2006 ◽

Vol 189 (1) ◽

pp. 220-227 ◽

Cited By ~ 30

Author(s):

James T. Riordan ◽

Arunachalam Muthaiyan ◽

Wayne Van Voorhies ◽

Christopher T. Price ◽

James E. Graham ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Resistance ◽

Transcriptome Analysis ◽

Target Genes ◽

Efflux Pump ◽

Multidrug Efflux ◽

Multidrug Efflux Pump ◽

Antibiotic Target ◽

Unique Mechanism ◽

Formate Metabolism

ABSTRACT Growth of Staphylococcus aureus with the nonsteroidal anti-inflammatory salicylate reduces susceptibility of the organism to multiple antimicrobials. Transcriptome analysis revealed that growth of S. aureus with salicylate leads to the induction of genes involved with gluconate and formate metabolism and represses genes required for gluconeogenesis and glycolysis. In addition, salicylate induction upregulates two antibiotic target genes and downregulates a multidrug efflux pump gene repressor (mgrA) and sarR, which represses a gene (sarA) important for intrinsic antimicrobial resistance. We hypothesize that these salicylate-induced alterations jointly represent a unique mechanism that allows S. aureus to resist antimicrobial stress and toxicity.

Download Full-text

Transcriptome analysis predicts mode of action of benzimidazole molecules against Staphylococcus aureus UAMS‐1

Drug Development Research ◽

10.1002/ddr.21523 ◽

2019 ◽

Vol 80 (4) ◽

pp. 490-503 ◽

Cited By ~ 6

Author(s):

Deepika Chauhan ◽

Santanu Hati ◽

Richa Priyadarshini ◽

Subhabrata Sen

Keyword(s):

Staphylococcus Aureus ◽

Transcriptome Analysis ◽

Mode Of Action

Download Full-text

RNA-Seq-based transcriptome analysis of methicillin-resistant Staphylococcus aureus biofilm inhibition by ursolic acid and resveratrol

Scientific Reports ◽

10.1038/srep05467 ◽

2014 ◽

Vol 4 (1) ◽

Cited By ~ 57

Author(s):

Nan Qin ◽

Xiaojuan Tan ◽

Yinming Jiao ◽

Lin Liu ◽

Wangsheng Zhao ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Ursolic Acid ◽

Transcriptome Analysis ◽

Methicillin Resistant Staphylococcus Aureus ◽

Rna Seq ◽

Biofilm Inhibition ◽

Methicillin Resistant

Download Full-text

Global Transcriptome Analysis of Staphylococcus aureus Response to Hydrogen Peroxide

Journal of Bacteriology ◽

10.1128/jb.188.4.1648-1659.2006 ◽

2006 ◽

Vol 188 (4) ◽

pp. 1648-1659 ◽

Cited By ~ 97

Author(s):

Wook Chang ◽

David A. Small ◽

Freshteh Toghrol ◽

William E. Bentley

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Staphylococcus Aureus ◽

Transcriptome Analysis ◽

Cellular Response ◽

Anaerobic Metabolism ◽

Oxidative Response ◽

Global Transcriptome ◽

Protective Strategies ◽

Reactive Oxidants

ABSTRACT Staphylococcus aureus responds with protective strategies against phagocyte-derived reactive oxidants to infect humans. Herein, we report the transcriptome analysis of the cellular response of S. aureus to hydrogen peroxide-induced oxidative stress. The data indicate that the oxidative response includes the induction of genes involved in virulence, DNA repair, and notably, anaerobic metabolism.

Download Full-text