Higginsianins D and E, Cytotoxic Diterpenoids Produced by Colletotrichum higginsianum

2020 ◽  
Vol 83 (4) ◽  
pp. 1131-1138
Author(s):  
Marco Masi ◽  
Alessio Cimmino ◽  
Flora Salzano ◽  
Roberta Di Lecce ◽  
Marcin Górecki ◽  
...  
Keyword(s):  
Colletotrichum Higginsianum

scholarly journals Isolation and Characterization of Extracellular Vesicles from the Fungal Phytopathogen Colletotrichum higginsianum

2022 ◽  
Author(s):  
Brian D Rutter ◽  
Thi-Thu-Huyen Chu ◽  
Kamil K Zajt ◽  
Jean-Felix Dallery ◽  
Richard J O'Connell ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Experimental System ◽  
Protein Markers ◽  
Cell Wall Biogenesis ◽  
Colletotrichum Higginsianum ◽  
Isolation And Characterization ◽  
Large Numbers ◽  
Transgenic Lines ◽  
Fungal Phytopathogen ◽  
Fungal Phytopathogens

Fungal phytopathogens secrete extracellular vesicles (EVs) associated with enzymes and phytotoxic metabolites. While these vesicles are thought to promote infection, defining the true contents and functions of fungal EVs, as well as suitable protein markers, is an ongoing process. To expand our understanding of fungal EVs and their possible roles during infection, we purified EVs from the hemibiotrophic phytopathogen Colletotrichum higginsianum, the causative agent of anthracnose disease in multiple plant species, including Arabidopsis thaliana. EVs were purified in large numbers from the supernatant of protoplasts but not the supernatant of intact mycelial cultures. We purified two separate populations of EVs, each associated with over 700 detected proteins, including proteins involved in vesicle transport, cell wall biogenesis and the synthesis of secondary metabolites. We selected two SNARE proteins (Snc1 and Sso2) and one 14-3-3 protein (Bmh1) as potential EV markers and generated transgenic lines expressing fluorescent fusions. Each marker was confirmed to be protected inside EVs. Fluorescence microscopy was used to examine the localization of each marker during infection on Arabidopsis leaves. These findings further our understanding of EVs in fungal phytopathogens and will help build an experimental system to study EV inter-kingdom communication between plants and fungi.

iTRAQ-based quantitative proteomics reveals ChAcb1 as a novel virulence factor in Colletotrichum higginsianum

2021 ◽  
Author(s):  
Yaqin Yan ◽  
Jintian Tang ◽  
Qinfeng Yuan ◽  
Liping Liu ◽  
Hao Liu ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Proteomic Analysis ◽  
Fungal Pathogens ◽  
Pathogenic Fungus ◽  
Virulence Gene ◽  
Melanin Biosynthesis ◽  
Functional Studies ◽  
Oxidative Stresses ◽  
Colletotrichum Higginsianum ◽  
Cruciferous Plants

Colletotrichum higginsianum is an important hemibiotrophic fungal pathogen that causes anthracnose disease on various cruciferous plants. Discovery of new virulence factors could lead to strategies for effectively controlling anthracnose. Acyl-CoA binding proteins (ACBPs) are mainly involved in binding and trafficking acyl-CoA esters in eukaryotic cells. However, the functions of this important class of proteins in plant fungal pathogens remain unclear. In this study, we performed an iTRAQ-based quantitative proteomic analysis to identify differentially expressed proteins (DEPs) between a nonpathogenic mutant ΔCh-MEL1 and the wild-type. Based on iTRAQ data, DEPs in the ΔCh-MEL1 mutant were mainly associated with melanin biosynthesis, carbohydrate and energy metabolism, lipid metabolism, redox processes, and amino acid metabolism. Proteomic analysis revealed that many DEPs might be involved in growth and pathogenesis of C. higginsianum. Among them, an acyl-CoA binding protein, ChAcb1, was selected for further functional studies. Deletion of ChAcb1 caused defects in vegetative growth and conidiation. ChAcb1 is also required for response to hyperosmotic and oxidative stresses, and maintenance of cell wall integrity. Importantly, the ΔChAcb1 mutant exhibited reduced virulence, and microscopic examination revealed that it was defective in appressorial penetration and infectious growth. Furthermore, the ΔChAcb1 mutant was impaired in fatty acid and lipid metabolism. Taken together, ChAcb1 was identified as a new virulence gene in this plant pathogenic fungus.

scholarly journals Colletotrichum higginsianum as a Model for Understanding Host–Pathogen Interactions: A Review

2018 ◽  
Vol 19 (7) ◽  
pp. 2142 ◽  
Author(s):  
Yaqin Yan ◽  
Qinfeng Yuan ◽  
Jintian Tang ◽  
Junbin Huang ◽  
Tom Hsiang ◽  
...  
Keyword(s):  
Genome Structure ◽  
Resistance Mechanisms ◽  
Integrated System ◽  
Plant Interactions ◽  
Infection Structure ◽  
Colletotrichum Higginsianum ◽  
Excellent Starting Point ◽  
Starting Point ◽  
Extensive Information ◽  
Ascomycetous Fungus

Colletotrichum higginsianum is a hemibiotrophic ascomycetous fungus that causes economically important anthracnose diseases on numerous monocot and dicot crops worldwide. As a model pathosystem, the Colletotrichum–Arabidopsis interaction has the significant advantage that both organisms can be manipulated genetically. The goal of this review is to provide an overview of the system and to point out recent significant studies that update our understanding of the pathogenesis of C. higginsianum and resistance mechanisms of Arabidopsis against this hemibiotrophic fungus. The genome sequence of C. higginsianum has provided insights into how genome structure and pathogen genetic variability has been shaped by transposable elements, and allows systematic approaches to longstanding areas of investigation, including infection structure differentiation and fungal–plant interactions. The Arabidopsis-Colletotrichum pathosystem provides an integrated system, with extensive information on the host plant and availability of genomes for both partners, to illustrate many of the important concepts governing fungal–plant interactions, and to serve as an excellent starting point for broad perspectives into issues in plant pathology.

Identification of virulence genes in the crucifer anthracnose fungus Colletotrichum higginsianum by insertional mutagenesis

2013 ◽  
Vol 64 ◽  
pp. 6-17 ◽  
Author(s):  
Liping Liu ◽  
Dian Zhao ◽  
Lu Zheng ◽  
Tom Hsiang ◽  
Yangdou Wei ◽  
...  
Keyword(s):  
Insertional Mutagenesis ◽  
Virulence Genes ◽  
Colletotrichum Higginsianum

scholarly journals Colletotrichum higginsianum extracellular LysM proteins play dual roles in appressorial function and suppression of chitin‐triggered plant immunity

2016 ◽  
Vol 211 (4) ◽  
pp. 1323-1337 ◽  
Author(s):  
Hiroyuki Takahara ◽  
Stéphane Hacquard ◽  
Anja Kombrink ◽  
H. Bleddyn Hughes ◽  
Vivek Halder ◽  
...  
Keyword(s):  
Plant Immunity ◽  
Dual Roles ◽  
Colletotrichum Higginsianum

scholarly journals The phagocytosis oxidase/Bem1p (PB1) domain-containing protein PB1CP negatively regulates the NADPH oxidase RBOHD in plant immunity

2020 ◽  
Author(s):  
Yukihisa Goto ◽  
Noriko Maki ◽  
Jan Sklenar ◽  
Paul Derbyshire ◽  
Frank L.H. Menke ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Stomatal Closure ◽  
Defense Responses ◽  
Mass Spectrometry Analysis ◽  
Regulation Mechanism ◽  
Cell Periphery ◽  
List Type ◽  
Colletotrichum Higginsianum ◽  
Associated Proteins ◽  
Pb1 Domain

SummaryPerception of pathogen-associated molecular patterns (PAMPs) by surface-localized pattern-recognition receptors activates RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) through direct phosphorylation by BOTRYTIS-INDUCED KINASE 1 (BIK1) and induces the production of reactive oxygen species (ROS). ROS have direct antimicrobial properties but also serve as signaling molecules to activate additional defense responses such as stomatal closure. RBOHD activity must be tightly controlled to avoid the detrimental effects of ROS, but little is known about RBOHD downregulation.To better understand the regulation of RBOHD, we used co-immunoprecipitation of RBOHD coupled with mass spectrometry analysis to identify RBOHD-associated proteins.Among RBOHD-associated proteins, we identified PHAGOCYTOSIS OXIDASE/ BEM1P (PB1) DOMAIN-CONTAINING PROTEIN (PB1CP). We found that PB1CP negatively regulates RBOHD and the resistance against the fungal pathogen Colletotrichum higginsianum. PB1CP directly interacts with RBOHD in vitro, and PAMP treatment increases the interaction in vivo. PB1CP is localized at the cell periphery and in cytoplasm, but PAMP treatment induces PB1CP relocalization to small endomembrane compartments. PB1CP overexpression reduces plasma membrane localization of RBOHD, suggesting that PB1CP down-regulates RBOHD function by relocalizing it away from the plasma membrane.We reveal a novel negative regulation mechanism of ROS production through the relocalization of RBOHD by PB1CP.

Enrichment of Phosphatidylinositol 4,5-Bisphosphate in the Extra-Invasive Hyphal Membrane Promotes Colletotrichum Infection of Arabidopsis thaliana

2019 ◽  
Vol 60 (7) ◽  
pp. 1514-1524 ◽  
Author(s):  
Takashi L Shimada ◽  
Shigeyuki Betsuyaku ◽  
Noriko Inada ◽  
Kazuo Ebine ◽  
Masaru Fujimoto ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Crucial Role ◽  
Pathogenic Fungi ◽  
Colletotrichum Higginsianum ◽  
Promising Target ◽  
Successful Infection ◽  
Phosphatidylinositol 4 ◽  
Protein Supply ◽  
Plant Plasma Membrane

Abstract Pathogenic fungi from the genus Colletotrichum form invasive hyphae; the hyphae are surrounded by an extra-invasive hyphal membrane (EIHM), which is continuous with the plant plasma membrane. Although the EIHM plays a crucial role as the interface between plant and fungal cells, its precise function during Colletotrichum infection remains elusive. Here, we show that enrichment of phosphoinositides (PIs) has a crucial role in Colletotrichum infection. We observed the localization of PIs in Arabidopsis thaliana cells infected by A. thaliana-adapted Colletotrichum higginsianum (Ch), and found that phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] was extremely enriched in the EIHM during Ch infection. We also found that phosphatidylinositol 4-phosphate-5 kinase (PIP5K), which catalyzes production of PI(4,5)P2, also accumulated at the EIHM. The overexpression of PIP5K3 in A. thaliana increased hyphal invasion by Ch. An exocytic factor, EXO84b, was targeted to the EIHM during Ch infection, although endocytic factors such as CLATHRIN LIGHT CHAIN 2 and FLOTILLIN 1 did not. Intriguingly, the interfacial membranes between A. thaliana and powdery mildew- or downy mildew-causing pathogens did not accumulate PI(4,5)P2. These results suggest that Ch could modify the PI(4,5)P2 levels in the EIHM to increase the exocytic membrane/protein supply of the EIHM for successful infection. Our results also suggest that PI(4,5)P2 biosynthesis is a promising target for improved defense against Colletotrichum infection.

scholarly journals A Dispensable Chromosome Is Required for Virulence in the Hemibiotrophic Plant Pathogen Colletotrichum higginsianum

2018 ◽  
Vol 9 ◽  
Author(s):  
Peter-Louis Plaumann ◽  
Johannes Schmidpeter ◽  
Marlis Dahl ◽  
Leila Taher ◽  
Christian Koch
Keyword(s):  
Plant Pathogen ◽  
Colletotrichum Higginsianum ◽  
Dispensable Chromosome

scholarly journals ChSte7 Is Required for Vegetative Growth and Various Plant Infection Processes inColletotrichum higginsianum

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Qinfeng Yuan ◽  
Meijuan Chen ◽  
Yaqin Yan ◽  
Qiongnan Gu ◽  
Junbin Huang ◽  
...  
Keyword(s):  
Vegetative Growth ◽  
Pathogenic Fungi ◽  
Colletotrichum Higginsianum ◽  
Plant Infection ◽  
Acid Protein ◽  
Leaf Tissues ◽  
Infection Processes ◽  
Late Stages ◽  
Host Tissues

Colletotrichum higginsianumis an important hemibiotrophic phytopathogen that causes crucifer anthracnose in various regions of the world. In many plant-pathogenic fungi, the Ste11-Ste7-Fus3/Kss1 kinase pathway is essential to pathogenicity and various plant infection processes. To date, the role of ChSte7 inC.higginsianumencoding a MEK orthologue of Ste7 inSaccharomyces cerevisiaehas not been elucidated. In this report, we investigated the function of ChSte7 in the pathogen. The ChSte7 is predicted to encode a 522-amino-acid protein with a S_TKc conserved domain that shares 44% identity with Ste7 inS.cerevisiae. ChSte7 disruption mutants showed white colonies with irregularly shaped edges and extremely decreased growth rates and biomass productions. The ChSte7 disruption mutants did not form appressoria and showed defects in pathogenicity on leaves ofArabidopsis thaliana. When inoculated onto wounded leaf tissues, the ChSte7 disruption mutants grew only on the surface of host tissues but failed to cause lesions beyond the wound site. In contrast, both the wild-type and complementation strains showed normal morphology, produced appressoria, and caused necrosis on leaves ofArabidopsis. Analysis with qRT-PCR suggested that ChSte7 was highly expressed during the late stages of infection. Taken together, our results demonstrate that ChSte7 is involved in regulation of vegetative growth, appressorial formation ofC.higginsianum, and postinvasive growth in host tissues.

Sign in / Sign up

Export Citation Format

Share Document