scholarly journals Silk Protein Solution: A Natural Example of Sticky Reptation

2020 ◽  
Vol 53 (7) ◽  
pp. 2669-2676 ◽  
Author(s):  
Charley Schaefer ◽  
Peter R. Laity ◽  
Chris Holland ◽  
Tom C. B. McLeish
Keyword(s):  
Silk Protein ◽  
Protein Solution

Extraction of Silk Protein from Middle Silk Gland of B.mori for Preparation of 3-D Scaffold

2012 ◽  
Vol 550-553 ◽  
pp. 1729-1736
Author(s):  
Ya Jun Shuai ◽  
Pan Hui ◽  
Wen He ◽  
Si Jia Min ◽  
Liang Jun Zhu ◽  
...  
Keyword(s):  
Pore Size ◽  
Water Retention ◽  
Retention Rate ◽  
Silk Gland ◽  
Silk Protein ◽  
Water Absorbency ◽  
Native Protein ◽  
Protein Solution ◽  
Low Concentration ◽  
Β Sheet

In this paper, 3-D scaffolds were prepared using native protein solution extracted from middle silk gland of B.mori silkworm (SS). The distribution of pore in 3-D scaffold was homogeneous, and the pore size decreased with increase in the concentration of SS. By changing the concentration from 1.7%, 3.5% to 7%, the porosity rate of scaffolds was 95%, 94% and 91%, respectively. The water absorbency apparently decreases and the water retention rate increases with increase in the concentration of SS. The scaffolds prepared from SS with concentration of 3.5% and 7% have higher strength, on the contrary, the scaffolds prepared from SS with low concentration 1.7% shows higher elasticity. FTIR spectra indicated that SS mainly adopted β-sheet conformation. This paper proposed one green way to extract silk protein from the silk gland of B.mori silkworm and prepare its corresponding scaffolds. It seems meaning to provide implication to develop silk-based biomaterials.

EFFICIENCY OF PRE-INCUBATIVE PROCESSING OF EGGS WITH EGG PROTEIN SOLUTION

2018 ◽  
pp. 112-124
Author(s):  
A.A. LINNIK ◽  
◽  
S.A. ALEKSEYEVА ◽  
O.Y. KUZNETSOV ◽  
◽  
...  
Keyword(s):  
Protein Solution ◽  
Egg Protein

Electrospun Mussel-derived Silk Fibers

2020 ◽  
Vol 14 (1) ◽  
pp. 14-20 ◽  
Author(s):  
Dan Tian ◽  
Dan-Ni Yu ◽  
Yi-Ming Xu ◽  
Xu-Yin Ding ◽  
Zhou-Yu Zhang ◽  
...  
Keyword(s):  
High Strength ◽  
Alcohol Solution ◽  
Protein Solution ◽  
High Mechanical Strength ◽  
Silk Fibers ◽  
Spider Dragline Silk ◽  
Blue Mussels ◽  
Wetting Properties ◽  
The World ◽  
Silkworm Silk

Background: Though there are many patents on silk, patents on sea silk are rare. Sea silk is one of the most coveted materials in the world, and the technology to make sea silk is at an extremely high risk of extinction. Unlike spider dragline silk and silkworm silk, this natural silk has been forgotten in the academic commune for millennia, though it has many fascinating properties: high strength, remarkable adhesion, extreme lightweight, and others. Method: Here we report that mussel-derived silk fibers can be fabricated by electrospinning. Instead of extracting proteins from byssus, we directly use the protein solution from alive blue mussels, which are intensely commercially used. The protein solution and the polyvinyl alcohol solution are mixed together to produce mussel-based silk fibers. Results: The mussel-based silk fibers have many special properties like high mechanical strength, remarkable super-contraction and good wetting properties. Conclusion: The electrospinning mussel-based silk fibers have the potential for use as a replacement for the rarest sea silk and as a new bio-inspired material with multi-functions.

scholarly journals Interfacial Crystallization and Supramolecular Self-Assembly of Spider Silk Inspired Protein at the Water-Air Interface

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4239
Author(s):  
Pezhman Mohammadi ◽  
Fabian Zemke ◽  
Wolfgang Wagermaier ◽  
Markus B. Linder
Keyword(s):  
Self Assembly ◽  
Spider Silk ◽  
Assembly Process ◽  
Protein Solution ◽  
Time Resolved ◽  
Macromolecular Assembly ◽  
X Ray Scattering ◽  
Air Interface ◽  
Fundamental Research ◽  
Β Sheet

Macromolecular assembly into complex morphologies and architectural shapes is an area of fundamental research and technological innovation. In this work, we investigate the self-assembly process of recombinantly produced protein inspired by spider silk (spidroin). To elucidate the first steps of the assembly process, we examined highly concentrated and viscous pendant droplets of this protein in air. We show how the protein self-assembles and crystallizes at the water–air interface into a relatively thick and highly elastic skin. Using time-resolved in situ synchrotron X-ray scattering measurements during the drying process, we showed that the skin evolved to contain a high β-sheet amount over time. We also found that β-sheet formation strongly depended on protein concentration and relative humidity. These had a strong influence not only on the amount, but also on the ordering of these structures during the β-sheet formation process. We also showed how the skin around pendant droplets can serve as a reservoir for attaining liquid–liquid phase separation and coacervation from the dilute protein solution. Essentially, this study shows a new assembly route which could be optimized for the synthesis of new materials from a dilute protein solution and determine the properties of the final products.

scholarly journals The Different Colors of mAbs in Solution

Antibodies ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 21
Author(s):  
Alexandre Ambrogelly
Keyword(s):  
Visual Inspection ◽  
Quantitative Methods ◽  
Geographical Area ◽  
Therapeutic Protein ◽  
Protein Solutions ◽  
Protein Solution ◽  
Critical Quality Attribute ◽  
Recombinant Therapeutic Protein ◽  
Protein Variants ◽  
Antibody Solution

The color of a therapeutic monoclonal antibody solution is a critical quality attribute. Consistency of color is typically assessed at time of release and during stability studies against preset criteria for late stage clinical and commercial products. A therapeutic protein solution’s color may be determined by visual inspection or by more quantitative methods as per the different geographical area compendia. The nature and intensity of the color of a therapeutic protein solution is typically determined relative to calibrated standards. This review covers the analytical methodologies used for determining the color of a protein solution and presents an overview of protein variants and impurities known to contribute to colored recombinant therapeutic protein solutions.

scholarly journals Changes in Amino Acid Profiles and Bioactive Compounds of Thai Silk Cocoons as Affected by Water Extraction

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2033
Author(s):  
Chuleeporn Bungthong ◽  
Sirithon Siriamornpun
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Silk Protein ◽  
Extraction Time ◽  
Antioxidant Power ◽  
Silk Cocoon ◽  
Amino Acid Profiles

Silk proteins have many advantageous components including proteins and pigments. The proteins—sericin and fibroin—have been widely studied for medical applications due to their good physiochemical properties and biological activities. Various strains of cocoon display different compositions such as amino-acid profiles and levels of antioxidant activity. Therefore, the objectives of this study were to find a suitable silk protein extraction method to obtain products with chemical and biological properties suitable as functional foods in two strains of Bombyx mori silk cocoon (Nangsew strains; yellow cocoon) and Samia ricini silk cocoon (Eri strains; white cocoon) extracted by water at 100 °C for 2, 4, 6 and 8 h. The results showed that Nangsew strains extracted for 6 h contained the highest amounts of protein, amino acids, total phenolics (TPC) and total flavonoids (TFC), plus DPPH radical-scavenging activity, ABTS radical scavenging capacity, and ferric reducing antioxidant power (FRAP), anti-glycation, α-amylase and α-glucosidase inhibition. The longer extraction time produced higher concentrations of amino acids, contributing to sweet and umami tastes in both silk strains. It seemed that the bitterness decreased as the extraction time increased, resulting in improvements in the sweetness and umami of silk-protein extracts.

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