Microarray Determination of the Expression of Drug Transporters in Humans and Animal Species Used for the Investigation of Nasal Absorption

This study was conducted at Dr. Taleb A. Jaayad of Molecular Genetics, Department of Animal Production, College of Agriculture, University of Basrah. Samples of fresh and canned meat of cattle, buffalo, sheep, goats, chicken and turkey were collected randomly from different areas of Basrah province, as well as blood samples of camel. The aim was to determine different animal species from their meat (except camel). DNA was extracted from meat tissue (0.2 gm) and blood by using DNA kit (Invetrogen). DNA purity was estimated by using wavelength (260-280), to be 1.8- 2.0 ng. PCR was used to amplify mtco1 gene using a general primer and gave a band of 710 bp for all species used in this study. Different species were determined by using Taq restricted enzyme. Cattle, buffalo, chicken and turkey showed one band of 637 bp. Taq enzyme has recognized sheep and goat, while sheep did not show any band to the fragment 710 bp. However, goat showed a band at 650 bp. Furthermore, camel produced two bands of 303 and 403 bp.

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Sensitivity of life-long diagnostic methods for geese nematodes

Ukrainian Journal of Veterinary and Agricultural Sciences ◽

10.32718/ujvas2-3.09 ◽

2019 ◽

Vol 2 (3) ◽

pp. 39-42

Author(s):

O. B. Prijma

Keyword(s):

Final Stage ◽

Comparative Evaluation ◽

Animal Species ◽

Prolonged Exposure ◽

Laboratory Diagnosis ◽

Diagnostic Methods ◽

Sugar Solution ◽

Main Indicator ◽

Nematode Eggs

The basis of successful struggle and specific prevention of poultry worms is timely diagnosis, the final stage of which is the detection of the worms themselves, their eggs or larvae at various stages of development. Priority is given to the methods of lifelong laboratory diagnosis of helminthiasis, which are preferably recommended for use in all animal species, including poultry. The aim of this work was to determine the sensitivity of flotation methods of coprovoscopy for geese nematodes. Experimental determination of the efficiency of the well-known methods of flotation and their comparative evaluation of coproovoscopic diagnosis of heterocosis, capillary disease and trichostrongilosis of geese. The main indicator of the diagnostic effectiveness of the methods was the intensity of the invasion and the time spent on the flotation of the samples. The most effective methods for diagnosing geese geoccosis are Kotelnikov-Hrenov (with ammonium nitrate) – at exposures of 20 min and Mallory (with saturated sugar solution) – at exposures of 10–15 min. The rates of invasion intensity were respectively 62.0 ± 4.39 and 59.0 ± 3.47 eggs/g. In the laboratory diagnosis of goose capillary disease, the most sensitive methods were Kotelnikov-Hrenov and Mallory at exposures of 15–20 min, where infestation rates reached 34.0 ± 2.22 and 33.5 ± 2.64 eggs/g, respectively. For trichostrongilosis, the Kotelnikov-Hrenov method showed the highest sensitivity at the exposure of 20 min, the intensity of the invasion was 32.5 ± 3.23 eggs/g. The Mallory method proved to be less effective – at an exposure of 15 min poultry invasiveness was 23.5 ± 1.81 eggs/g. The least sensitive of this invasion was the Fulleborn method (with NaCl), where the intensity of the invasion gradually increased with prolonged exposure and ranged from 10.5 ± 0.5 to 19.5 ± 2.45 eggs/g. Based on the data obtained, it is recommended to use the most sensitive methods and to take into account the exposure, which ensures the concentration of the largest number of nematode eggs on the surface of the flotant when conducting life-long coproovoscopic diagnostics of heterosis, capillary disease and trichostrongilosis of geese.

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First serological record of Coxiella burnetii infection in the equine population of Slovakia

Biologia ◽

10.1007/s11756-021-00898-4 ◽

2021 ◽

Author(s):

Monika Drážovská ◽

Marián Prokeš ◽

Boris Vojtek ◽

Jana Mojžišová ◽

Anna Ondrejková ◽

...

Keyword(s):

16S Rrna ◽

Coxiella Burnetii ◽

Animal Species ◽

Q Fever ◽

Statistical Significance ◽

Elisa Method ◽

Zoonotic Pathogen ◽

Specific Antibodies

AbstractCoxiella burnetii is a worldwide zoonotic pathogen causing Q fever in various animal species and humans. In Slovakia, cases of C. burnetii infection in both animals and humans are confirmed every year. The role of horses in the epidemiology of this neglected disease is still unclear. In our study, we focused on a serosurvey of C. burnetii in the equine population in Slovakia by the ELISA method. Subsequently, a nested PCR was performed to detect the 16S rRNA fragment of the genus Coxiella. Among 184 horse sera, the presence of specific antibodies to C. burnetii was detected in four samples, representing a 2.17% seropositivity. All the positive horses were mares; two originated from Central Slovakia and two from Eastern Slovakia. Although the number of positive samples was too small for a determination of statistical significance, our results provide the first confirmation of antibodies to C. burnetii in horses from Slovakia. Although no positive PCR result was obtained, these serological findings may help to clarify the circulation of the pathogen in the environment.

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Design of a flow cytometric assay for the determination of natural killer and cytotoxic T-lymphocyte activity in human and in different animal species

Cytometry ◽

10.1002/1097-0320(20001201)41:4<289::aid-cyto7>3.0.co;2-5 ◽

2000 ◽

Vol 41 (4) ◽

pp. 289-297 ◽

Cited By ~ 70

Author(s):

L. Piriou ◽

S. Chilmonczyk ◽

N. Genetet ◽

E. Albina

Keyword(s):

Natural Killer ◽

T Lymphocyte ◽

Animal Species ◽

Cytotoxic T Lymphocyte ◽

Flow Cytometric Assay ◽

Flow Cytometric ◽

Lymphocyte Activity

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Confirmatory Method for the Determination of Amphenicols in Muscle and Kidney of Several Animal Species

Food Analytical Methods ◽

10.1007/s12161-016-0623-2 ◽

2016 ◽

Vol 10 (3) ◽

pp. 610-617 ◽

Cited By ~ 1

Author(s):

Francisco Moragues ◽

Carmen Igualada ◽

Nuria León

Keyword(s):

Animal Species

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The relative resistance to escape of leaf and stem particles from the rumen of cattle and sheep

The Journal of Agricultural Science ◽

10.1017/s0021859600055623 ◽

1985 ◽

Vol 105 (1) ◽

pp. 9-14 ◽

Cited By ~ 55

Author(s):

D. P. Poppi ◽

R. E. Hendricksen ◽

D. J. Minson

Keyword(s):

Particle Size ◽

Animal Species ◽

Particle Sizes ◽

Small Particles ◽

Relative Resistance ◽

Stages Of Growth ◽

Two Stages ◽

Wet Sieving ◽

Cattle And Sheep

SUMMARYIn a study of the effect of animal species on the threshold particle size leaving the rumen, two grasses cut at two stages of growth and one mature legume were separated into leaf and stem fractions and fed to cattle and sheep. Samples of rumen digesta and faeces were used to determine the validity of using a 1·18 mm porosity screen to separate the rumen particles into large and small pools when studying escape of particles from the rumen. Samples of rumen digesta and faeces were collected for the determination of particle size by wet sieving and the calculation of resistance of particles to passage from the rumen relative to small particles retained on a 0·15 mm sieve.Particles < 1·18 mm but > 0·5 mm had a mean relative resistance to passage of 2·0 and 2·6 for cattle and sheep respectively, compared with resistance values of between 10·9 and 31·2 for particles between 1·18 and 2·36 mm. It is suggested that there is no justification for using different threshold particle sizes for sheep and cattle and that a 1·18 mm sieve may be used to divide the rumen contents of both cattle and sheep into two pools of particles with high and low relative resistance to passage from the rumen.

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Determination and comparative analysis of the catalytic subunit of adenosine 3′,5′-cyclic phosphate-dependent protein kinase by an enzyme-linked immunosorbent assay

Biochemical Journal ◽

10.1042/bj2080109 ◽

1982 ◽

Vol 208 (1) ◽

pp. 109-117 ◽

Cited By ~ 8

Author(s):

G Schwoch ◽

A Hamann

Keyword(s):

Immunosorbent Assay ◽

Animal Species ◽

Catalytic Subunit ◽

Enzyme Linked Immunosorbent Assay ◽

Dependent Protein Kinase ◽

Bovine Heart ◽

Lower Detection ◽

Dependent Protein ◽

Cyclic Phosphate

A specific antiserum against bovine heart catalytic subunit was used for the determination of the catalytic subunit in an enzyme-linked immunosorbent assay. Under the conditions elaborated the assay has a lower detection limit for catalytic subunit of 0.25 pmol/ml. In crude bovine heart extracts the concentration of catalytic subunit was determined by this method to be 0.18 +/- 0.02 mumol/kg wet wt. The immunochemical comparison of various animal species and cells, including organisms like amoebae and yeast, shows the broad applicability of the assay and provides evidence that the catalytic subunit is a highly conserved molecule.

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